Different leaf extracts from Brunfelsia uniflora in mice

• Autor(a) principal: Carvalho,Ciro José Sousa de
• Data de Publicação: 2018
• Outros Autores: Melo,Marília Martins, Michell,Ana Flávia Ribeiro Machado, Torres,Bruno Benetti Junta, Riet-Correa,Franklin, Guedes,Roberto Maurício Carvalho, Ferraz,Vany Perpetua, Soto-Blanco,Benito, Silva,Silvana Maria Medeiros de Sousa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000100552
• topic: toxicity; alkaloids; saponins; flavonoids; Brunfelsia; Solanaceae

ABSTRACT: In Brazil, at least 16 poisonous plant species can affect the central nervous system of livestock. Recently in the state of Piauí, Northeastern Brazil, Brunfelsia uniflora was reported as a cause of nervous signs in donkeys, cattle, and small ruminants. In order to assess the toxicity of B. uniflora extracts, 20 Swiss mice were distributed into four groups (n=5) receiving by gavage alkaloids, flavonoids, saponins, or saline (control group). After administration of the extracts in a single dose (5g kg-1), all mice were observed daily for clinical signs. Mice that received the extracts showed moderate to severe clinical signs, including piloerection, vocalization, and seizures. All mice dosed with saponins died between 10 and 20min after administration. Serum biochemical evaluation of animals that received saponins revealed slight increases in total protein levels and decreased magnesium and chlorite levels. In conclusion, saponins of B. uniflora leaves induced acute toxic neurological effects and death in mice.