Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
Resumo: | ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0 and 729.5x103 cells mL-1, respectively at M1, M2 and M3. Furthermore, considering the drop in sensitivity throughout the experimental time points, the use of serial bacterial cultures are recommended, particularly in herds with a high prevalence of S. aureus. |
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Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureusbacterial cultureintramammary infectionsmall ruminantsomatic cell countABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0 and 729.5x103 cells mL-1, respectively at M1, M2 and M3. Furthermore, considering the drop in sensitivity throughout the experimental time points, the use of serial bacterial cultures are recommended, particularly in herds with a high prevalence of S. aureus.Universidade Federal de Santa Maria2016-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217Ciência Rural v.46 n.7 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150507info:eu-repo/semantics/openAccessPeixoto,Rodolfo de MoraesAraújo,Renata de Moraes PeixotoPeixoto,Luciana Jatobá e SilvaReges,Ariel MarquesAlves,Ana Paula PereiraPinheiro Júnior,José WiltonMota,Rinaldo AparecidoAzevedo,Sérgio SantosCosta,Mateus Matiuzzi daeng2016-05-03T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
title |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
spellingShingle |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus Peixoto,Rodolfo de Moraes bacterial culture intramammary infection small ruminant somatic cell count |
title_short |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
title_full |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
title_fullStr |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
title_full_unstemmed |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
title_sort |
Indirect diagnostic tests for the detection of subclinical mastitis in dairy goats experimentally infected with Staphylococcus aureus |
author |
Peixoto,Rodolfo de Moraes |
author_facet |
Peixoto,Rodolfo de Moraes Araújo,Renata de Moraes Peixoto Peixoto,Luciana Jatobá e Silva Reges,Ariel Marques Alves,Ana Paula Pereira Pinheiro Júnior,José Wilton Mota,Rinaldo Aparecido Azevedo,Sérgio Santos Costa,Mateus Matiuzzi da |
author_role |
author |
author2 |
Araújo,Renata de Moraes Peixoto Peixoto,Luciana Jatobá e Silva Reges,Ariel Marques Alves,Ana Paula Pereira Pinheiro Júnior,José Wilton Mota,Rinaldo Aparecido Azevedo,Sérgio Santos Costa,Mateus Matiuzzi da |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Peixoto,Rodolfo de Moraes Araújo,Renata de Moraes Peixoto Peixoto,Luciana Jatobá e Silva Reges,Ariel Marques Alves,Ana Paula Pereira Pinheiro Júnior,José Wilton Mota,Rinaldo Aparecido Azevedo,Sérgio Santos Costa,Mateus Matiuzzi da |
dc.subject.por.fl_str_mv |
bacterial culture intramammary infection small ruminant somatic cell count |
topic |
bacterial culture intramammary infection small ruminant somatic cell count |
description |
ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0 and 729.5x103 cells mL-1, respectively at M1, M2 and M3. Furthermore, considering the drop in sensitivity throughout the experimental time points, the use of serial bacterial cultures are recommended, particularly in herds with a high prevalence of S. aureus. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20150507 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.7 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140549394235392 |