Inoculation methods and agressiveness of Macrophomina phaseolina isolates in cowpea

• Autor(a) principal: Souza,Erik Micael da Silva
• Data de Publicação: 2022
• Outros Autores: Silva,Leonardo Aparecido Brandão da, Pinto,Francisco Álef Carlos, Borel,Jerônimo Constantino, Capucho,Alexandre Sandri, Ishikawa,Francine Hiromi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000600402
• topic: charcoal rot; toothpick method; phenotyping; Vigna unguiculata (L) Walp; semiarid

ABSTRACT: The fungi Macrophomina phaseolina is the charcoal rot causal agent, one of the most important cowpea crop disease in semiarid regions can causes 100% yield losses. The search for resistant genotypes requires efficient phenotyping. In addition, there is the problem of great variation in aggressiveness between isolates. This study aimed to 1) test three methods of inoculation in semiarid conditions, and 2) to evaluate the aggressiveness of isolates of M. phaseolina. In the first experiment carried out in greenhouse, the inoculations methods were evaluated, using two cowpea lines, three inoculation methods and three pathogen isolates. On the second experiment, fifteen M. phaseolina isolates were inoculated in one cultivar to evaluate their aggressiveness. By assessing the length of the lesions and the severity of the disease using an index, we identified the toothpick inoculation method as the most efficient. Toothpick method allowed to discriminate the genotypes and the aggressiveness of the pathogen.