2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403 |
Resumo: | ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. |
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Ciência rural (Online) |
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2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.Cultivar Mundo Novo2-iPsomatic embryosembryogenic structuresABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.Universidade Federal de Santa Maria2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403Ciência Rural v.48 n.11 2018reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20180001info:eu-repo/semantics/openAccessAlves,Ivanilda dos SantosCarmazini,Valéria Cristina BarbosaSantos,Cosme Damião dosAlmeida,Julieta Andrea Silva deeng2018-11-12T00:00:00ZRevista |
dc.title.none.fl_str_mv |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
spellingShingle |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. Alves,Ivanilda dos Santos Cultivar Mundo Novo 2-iP somatic embryos embryogenic structures |
title_short |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_full |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_fullStr |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_full_unstemmed |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_sort |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
author |
Alves,Ivanilda dos Santos |
author_facet |
Alves,Ivanilda dos Santos Carmazini,Valéria Cristina Barbosa Santos,Cosme Damião dos Almeida,Julieta Andrea Silva de |
author_role |
author |
author2 |
Carmazini,Valéria Cristina Barbosa Santos,Cosme Damião dos Almeida,Julieta Andrea Silva de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Alves,Ivanilda dos Santos Carmazini,Valéria Cristina Barbosa Santos,Cosme Damião dos Almeida,Julieta Andrea Silva de |
dc.subject.por.fl_str_mv |
Cultivar Mundo Novo 2-iP somatic embryos embryogenic structures |
topic |
Cultivar Mundo Novo 2-iP somatic embryos embryogenic structures |
description |
ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20180001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.48 n.11 2018 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140553022308352 |