In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/41400 |
Resumo: | Dipteryx alata Vogel is a native species of the Cerrado domain with economic potential for use as a timber and food source. The present study aimed to establish an in vitro germination and shoot proliferation protocol for this species for future rooting and acclimatization investigations. Fruits were collected in October 2013. For asepsis and germination, the seeds were divided into two groups: in the first the seed coat was removed and in the second, maintained. Both groups were submitted to asepsis with detergent and 70% alcohol, followed by different concentrations of sodium hypochlorite (NaClO) (2.5% active chlorine) (v/v): 00%; 10%; 50%; and 100%. Two experiments were conducted for in vitro shoot proliferation: conventional lids or natural ventilation. Nodal segments from plants germinated in vitro (two months old) were inoculated with different concentrations of 6-Benzylaminopurine (BAP) (0.00 µM; 4.44 µM; and 11.10 µM) combined with naphthalene acetic acid (NAA) (0.00 µM; 2.69 µM; and 5.37 µM). The seeds were successfully decontaminated, with those exhibiting an intact seed coat and 50% NaClO found to be superior. High shoot proliferation was observed under natural ventilation, with 4.44 µM BAP (average of 4.03 shoots), a 101.50% increase in relation to controls and 43.93% when compared to the best conventional system treatment. On the other hand, this last system produced taller shoots under 2.69 and 5.37 µM of NAA (average of 19.42 and 18.18 mm, respectively), and 2.69 µM of NAA combined with 4.44 µM of BAP (average of 18.46 mm). By contrast, natural ventilation resulted in taller shoots for 5.37 µM of NAA combined with 11.10 µM of BAP (average of 12.81 mm). |
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In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilationGerminação in vitro de Dipteryx alata Vogel (Fabaceae) e proliferação de brotos sob sistema de ventilação convencional e naturalBaruCerradoPlant growth regulatorsIn vitro cultureBaruCerradoReguladores de crescimentoCultivo in vitroDipteryx alata Vogel is a native species of the Cerrado domain with economic potential for use as a timber and food source. The present study aimed to establish an in vitro germination and shoot proliferation protocol for this species for future rooting and acclimatization investigations. Fruits were collected in October 2013. For asepsis and germination, the seeds were divided into two groups: in the first the seed coat was removed and in the second, maintained. Both groups were submitted to asepsis with detergent and 70% alcohol, followed by different concentrations of sodium hypochlorite (NaClO) (2.5% active chlorine) (v/v): 00%; 10%; 50%; and 100%. Two experiments were conducted for in vitro shoot proliferation: conventional lids or natural ventilation. Nodal segments from plants germinated in vitro (two months old) were inoculated with different concentrations of 6-Benzylaminopurine (BAP) (0.00 µM; 4.44 µM; and 11.10 µM) combined with naphthalene acetic acid (NAA) (0.00 µM; 2.69 µM; and 5.37 µM). The seeds were successfully decontaminated, with those exhibiting an intact seed coat and 50% NaClO found to be superior. High shoot proliferation was observed under natural ventilation, with 4.44 µM BAP (average of 4.03 shoots), a 101.50% increase in relation to controls and 43.93% when compared to the best conventional system treatment. On the other hand, this last system produced taller shoots under 2.69 and 5.37 µM of NAA (average of 19.42 and 18.18 mm, respectively), and 2.69 µM of NAA combined with 4.44 µM of BAP (average of 18.46 mm). By contrast, natural ventilation resulted in taller shoots for 5.37 µM of NAA combined with 11.10 µM of BAP (average of 12.81 mm).Dipteryx alata Vogel é uma espécie nativa do domínio Cerrado, a qual possui potencial econômico de uso madeireiro e alimentício. Objetivou-se estabelecer um protocolo de germinação e proliferação de brotos in vitro desta espécie, visando futuros estudos de enraizamento e aclimatização. Frutos foram coletados em outubro de 2013. Para assepsia e germinação, as sementes foram divididas em dois grupos: no primeiro, o tegumento foi retirado, e no segundo este foi mantido. Os dois grupos foram submetidos à assepsia com detergente e álcool 70%, e em seguida a distintas concentrações de hipoclorito de sódio (NaClO) (2,5% de cloro ativo) (v/v): 00%; 10%; 50%; e 100%. Para proliferação de brotos in vitro, dois experimentos foram conduzidos: tampas convencionais ou ventilação natural. Segmentos nodais oriundos de plantas germinadas in vitro (dois meses de idade) foram inoculados em diferentes concentrações de 6-Benzilaminopurina (BAP) (0,00 µM; 4,44 µM; e 11,10 µM) combinadas a ácido naftaleno-acético (ANA) (0,00 µM; 2,69 µM; e 5,37 µM). A descontaminação de sementes foi realizada com sucesso, observando-se superioridade de sementes com presença de tegumento e 50% de NaClO. Alta proliferação de brotos foi observada em ventilação natural, com 4,44 µM de BAP (média de 4,03 brotos), um aumento de 101,50% em relação ao controle e 43,93% em relação ao melhor tratamento do sistema convencional. Por outro lado, este último sistema propiciou brotos com maiores alturas em 2,69 e 5,37 µM de ANA (média de 19,42 e 18,18 mm respectivamente), assim como em 2,69 µM de ANA combinado a 4,44 µM de BAP (média de 18,46 mm). Por outro lado, o sistema de ventilação natural mostrou brotos mais altos em 5,37 µM de ANA combinado a 11,10 µM de BAP (média de 12,81 mm).Universidade Federal de Santa Maria2022-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmltext/xmlhttps://periodicos.ufsm.br/cienciaflorestal/article/view/4140010.5902/1980509841400Ciência Florestal; Vol. 32 No. 2 (2022); 996-1010Ciência Florestal; v. 32 n. 2 (2022); 996-10101980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMenghttps://periodicos.ufsm.br/cienciaflorestal/article/view/41400/47554https://periodicos.ufsm.br/cienciaflorestal/article/view/41400/48540https://periodicos.ufsm.br/cienciaflorestal/article/view/41400/50786Copyright (c) 2022 Ciência Florestalhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilveira, Andreia Alves da CostaSilva, Lívia Cristina daSibov, Sérgio Tadeu2022-12-16T18:29:07Zoai:ojs.pkp.sfu.ca:article/41400Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2022-12-16T18:29:07Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation Germinação in vitro de Dipteryx alata Vogel (Fabaceae) e proliferação de brotos sob sistema de ventilação convencional e natural |
title |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
spellingShingle |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation Silveira, Andreia Alves da Costa Baru Cerrado Plant growth regulators In vitro culture Baru Cerrado Reguladores de crescimento Cultivo in vitro |
title_short |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
title_full |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
title_fullStr |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
title_full_unstemmed |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
title_sort |
In vitro germination and shoot proliferation of Dipteryx alata Vogel (Fabaceae) under conventional and natural ventilation |
author |
Silveira, Andreia Alves da Costa |
author_facet |
Silveira, Andreia Alves da Costa Silva, Lívia Cristina da Sibov, Sérgio Tadeu |
author_role |
author |
author2 |
Silva, Lívia Cristina da Sibov, Sérgio Tadeu |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silveira, Andreia Alves da Costa Silva, Lívia Cristina da Sibov, Sérgio Tadeu |
dc.subject.por.fl_str_mv |
Baru Cerrado Plant growth regulators In vitro culture Baru Cerrado Reguladores de crescimento Cultivo in vitro |
topic |
Baru Cerrado Plant growth regulators In vitro culture Baru Cerrado Reguladores de crescimento Cultivo in vitro |
description |
Dipteryx alata Vogel is a native species of the Cerrado domain with economic potential for use as a timber and food source. The present study aimed to establish an in vitro germination and shoot proliferation protocol for this species for future rooting and acclimatization investigations. Fruits were collected in October 2013. For asepsis and germination, the seeds were divided into two groups: in the first the seed coat was removed and in the second, maintained. Both groups were submitted to asepsis with detergent and 70% alcohol, followed by different concentrations of sodium hypochlorite (NaClO) (2.5% active chlorine) (v/v): 00%; 10%; 50%; and 100%. Two experiments were conducted for in vitro shoot proliferation: conventional lids or natural ventilation. Nodal segments from plants germinated in vitro (two months old) were inoculated with different concentrations of 6-Benzylaminopurine (BAP) (0.00 µM; 4.44 µM; and 11.10 µM) combined with naphthalene acetic acid (NAA) (0.00 µM; 2.69 µM; and 5.37 µM). The seeds were successfully decontaminated, with those exhibiting an intact seed coat and 50% NaClO found to be superior. High shoot proliferation was observed under natural ventilation, with 4.44 µM BAP (average of 4.03 shoots), a 101.50% increase in relation to controls and 43.93% when compared to the best conventional system treatment. On the other hand, this last system produced taller shoots under 2.69 and 5.37 µM of NAA (average of 19.42 and 18.18 mm, respectively), and 2.69 µM of NAA combined with 4.44 µM of BAP (average of 18.46 mm). By contrast, natural ventilation resulted in taller shoots for 5.37 µM of NAA combined with 11.10 µM of BAP (average of 12.81 mm). |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/41400 10.5902/1980509841400 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/41400 |
identifier_str_mv |
10.5902/1980509841400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/41400/47554 https://periodicos.ufsm.br/cienciaflorestal/article/view/41400/48540 https://periodicos.ufsm.br/cienciaflorestal/article/view/41400/50786 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html text/xml |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 32 No. 2 (2022); 996-1010 Ciência Florestal; v. 32 n. 2 (2022); 996-1010 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944135232192512 |