Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.

Detalhes bibliográficos
Autor(a) principal: Golle, Diego Pascoal
Data de Publicação: 2020
Outros Autores: Reiniger, Lia Rejane Silveira, Stefanel, Charlene Moro, Serrote, Caetano Miguel Lemos, Rabaiolli, Silvia Machado dos Santos, Silva, Karol Buuron da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/26987
Resumo: Eugenia involucrata is a native forest species from Brazil with great potential for timber, fruit and medical purpouses. This study aimed to evaluate the influence of growth regulators and the effect of light on calli induction from leaves disks of Eugenia involucrata. A bifactorial experiment (2x5) was conducted, in which the treatments consisted in the combinations of absence or presence of light during cultivation and five different uses of phytoregulators: NAA, 2,4-D, BAP and combinations of NAA and BAP, 2,4-D and BAP, all at 5 μM, in the nutritious medium MS. After 45 days of in vitro cultivation, it was observed that the absence of light during the cultivation period reduced phenolic oxidation, thus stimulated calogenesis, mainly in the presence of ANA and the combinations NAA + BAP and 2,4-D + BAP. The use of 2,4-D and BAP, on isolated way, did not favor calli formation in leaves disks of Eugenia involucrata.
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spelling Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.Fitorreguladores e luminosidade na indução à calogênese em explantes foliares de Eugenia involucrata DC.Calli cultureTissue cultureMyrtaceaeCultivo de calosCultura de tecidosMyrtaceaeEugenia involucrata is a native forest species from Brazil with great potential for timber, fruit and medical purpouses. This study aimed to evaluate the influence of growth regulators and the effect of light on calli induction from leaves disks of Eugenia involucrata. A bifactorial experiment (2x5) was conducted, in which the treatments consisted in the combinations of absence or presence of light during cultivation and five different uses of phytoregulators: NAA, 2,4-D, BAP and combinations of NAA and BAP, 2,4-D and BAP, all at 5 μM, in the nutritious medium MS. After 45 days of in vitro cultivation, it was observed that the absence of light during the cultivation period reduced phenolic oxidation, thus stimulated calogenesis, mainly in the presence of ANA and the combinations NAA + BAP and 2,4-D + BAP. The use of 2,4-D and BAP, on isolated way, did not favor calli formation in leaves disks of Eugenia involucrata.Eugenia involucrata é uma espécie florestal nativa do Brasil com grande potencial para uso madeireiro, frutícola e medicinal. O presente estudo objetivou avaliar a influência de fitorreguladores e da luminosidade sobre a calogênese em discos foliares de Eugenia involucrata. Foi conduzido um experimento bifatorial (2x5), cujos tratamentos consistiram na combinação da ausência ou presença de luz durante o cultivo e de cinco diferentes empregos de fitorreguladores: ANA, 2,4-D, BAP e as combinações ANA + BAP e 2,4-D + BAP, todos a 5 μM, em meio nutritivo MS. Após 45 dias de cultivo in vitro foi observado que a ausência de luz durante o período de cultivo reduz a oxidação fenólica favorecendo, assim, a calogênese , especialmente na presença de ANA e das combinações ANA + BAP e 2,4-D + BAP. O uso de 2,4-D e de BAP, isoladamente, não é favorável à calogênese em discos foliares de Eugenia involucrata.Universidade Federal de Santa Maria2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/2698710.5902/1980509826987Ciência Florestal; Vol. 30 No. 3 (2020); 898-906Ciência Florestal; v. 30 n. 3 (2020); 898-9061980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/26987/33592Copyright (c) 2020 Ciência Florestalinfo:eu-repo/semantics/openAccessGolle, Diego PascoalReiniger, Lia Rejane SilveiraStefanel, Charlene MoroSerrote, Caetano Miguel LemosRabaiolli, Silvia Machado dos SantosSilva, Karol Buuron da2020-09-02T20:52:06Zoai:ojs.pkp.sfu.ca:article/26987Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2020-09-02T20:52:06Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
Fitorreguladores e luminosidade na indução à calogênese em explantes foliares de Eugenia involucrata DC.
title Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
spellingShingle Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
Golle, Diego Pascoal
Calli culture
Tissue culture
Myrtaceae
Cultivo de calos
Cultura de tecidos
Myrtaceae
title_short Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
title_full Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
title_fullStr Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
title_full_unstemmed Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
title_sort Phytoregulators and lightness in the induction of calli in leaves explants of Eugenia involucrata DC.
author Golle, Diego Pascoal
author_facet Golle, Diego Pascoal
Reiniger, Lia Rejane Silveira
Stefanel, Charlene Moro
Serrote, Caetano Miguel Lemos
Rabaiolli, Silvia Machado dos Santos
Silva, Karol Buuron da
author_role author
author2 Reiniger, Lia Rejane Silveira
Stefanel, Charlene Moro
Serrote, Caetano Miguel Lemos
Rabaiolli, Silvia Machado dos Santos
Silva, Karol Buuron da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Golle, Diego Pascoal
Reiniger, Lia Rejane Silveira
Stefanel, Charlene Moro
Serrote, Caetano Miguel Lemos
Rabaiolli, Silvia Machado dos Santos
Silva, Karol Buuron da
dc.subject.por.fl_str_mv Calli culture
Tissue culture
Myrtaceae
Cultivo de calos
Cultura de tecidos
Myrtaceae
topic Calli culture
Tissue culture
Myrtaceae
Cultivo de calos
Cultura de tecidos
Myrtaceae
description Eugenia involucrata is a native forest species from Brazil with great potential for timber, fruit and medical purpouses. This study aimed to evaluate the influence of growth regulators and the effect of light on calli induction from leaves disks of Eugenia involucrata. A bifactorial experiment (2x5) was conducted, in which the treatments consisted in the combinations of absence or presence of light during cultivation and five different uses of phytoregulators: NAA, 2,4-D, BAP and combinations of NAA and BAP, 2,4-D and BAP, all at 5 μM, in the nutritious medium MS. After 45 days of in vitro cultivation, it was observed that the absence of light during the cultivation period reduced phenolic oxidation, thus stimulated calogenesis, mainly in the presence of ANA and the combinations NAA + BAP and 2,4-D + BAP. The use of 2,4-D and BAP, on isolated way, did not favor calli formation in leaves disks of Eugenia involucrata.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/26987
10.5902/1980509826987
url https://periodicos.ufsm.br/cienciaflorestal/article/view/26987
identifier_str_mv 10.5902/1980509826987
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/26987/33592
dc.rights.driver.fl_str_mv Copyright (c) 2020 Ciência Florestal
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Ciência Florestal
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 30 No. 3 (2020); 898-906
Ciência Florestal; v. 30 n. 3 (2020); 898-906
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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