Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/69093 |
Resumo: | Drimys brasiliensis Miers, popularly known as cataia, is a tree species native to the Atlantic Forest that has phytochemical, phytotherapeutic, aromatic and economic importance, with wide use in folk medicine. It is considered a species of difficult multiplication via seeds, presenting dormancy due to embryonic immaturity. Thus, the objective of this study was to analyze the vegetative rescue, in vitro establishment and propagation by way of cuttings of Drimys brasiliensis. For the rescue, the girdling techniques were applied at 30 and 90 cm from the ground, semi-girdling at 30 cm from the ground and pruned branches conditioned vertically and horizontally, being evaluated the shoot emission capacity. With the shoots obtained by vegetative rescue, the explants used for in vitro establishment were made, evaluating different immersion times in sodium hypochlorite 1.0 % (v/v) (0, 10, 15 and 20 minutes). The percentages of total, bacterial, fungal contamination and phenolic oxidation were evaluated. On the cuttings, shoots from vegetative rescue, from the aerial part of an adult individual and from detached branches were tested and placed in a mini-tunnel. The percentage of cuttings survival, percentage of cuttings with callus and percentage of rooting were evaluated. Regarding the vegetative rescue, the technique of pruned branches resulted in the induction of shoots, highlighting the branches conditioned vertically (100%). In the in vitro establishment, the immersion in sodium hypochlorite (1%) for 20 minutes proved to be efficient in the control of fungal contamination, however, no significant differences were observed in relation to the control of bacterial contamination. In cuttings, the use of cuttings from pruned branches or girdling/semi-girdling showed potential for these techniques in the cloning of the species, obtaining results close to 10% rooting. In general, the species has the potential for vegetative propagation, however, adjustments are necessary in the in vitro establishment and cutting protocols. |
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Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis MiersResgate vegetativo, estabelecimento in vitro e estaquia de Drimys brasiliensis MiersPropagação vegetativaRejuvenescimentoCultivo in vitroEspécie nativaVegetative propagationRejuvenationIn vitro cultivationNative speciesDrimys brasiliensis Miers, popularly known as cataia, is a tree species native to the Atlantic Forest that has phytochemical, phytotherapeutic, aromatic and economic importance, with wide use in folk medicine. It is considered a species of difficult multiplication via seeds, presenting dormancy due to embryonic immaturity. Thus, the objective of this study was to analyze the vegetative rescue, in vitro establishment and propagation by way of cuttings of Drimys brasiliensis. For the rescue, the girdling techniques were applied at 30 and 90 cm from the ground, semi-girdling at 30 cm from the ground and pruned branches conditioned vertically and horizontally, being evaluated the shoot emission capacity. With the shoots obtained by vegetative rescue, the explants used for in vitro establishment were made, evaluating different immersion times in sodium hypochlorite 1.0 % (v/v) (0, 10, 15 and 20 minutes). The percentages of total, bacterial, fungal contamination and phenolic oxidation were evaluated. On the cuttings, shoots from vegetative rescue, from the aerial part of an adult individual and from detached branches were tested and placed in a mini-tunnel. The percentage of cuttings survival, percentage of cuttings with callus and percentage of rooting were evaluated. Regarding the vegetative rescue, the technique of pruned branches resulted in the induction of shoots, highlighting the branches conditioned vertically (100%). In the in vitro establishment, the immersion in sodium hypochlorite (1%) for 20 minutes proved to be efficient in the control of fungal contamination, however, no significant differences were observed in relation to the control of bacterial contamination. In cuttings, the use of cuttings from pruned branches or girdling/semi-girdling showed potential for these techniques in the cloning of the species, obtaining results close to 10% rooting. In general, the species has the potential for vegetative propagation, however, adjustments are necessary in the in vitro establishment and cutting protocols.Drimys brasiliensis Miers, conhecida popularmente por cataia, é uma espécie arbórea nativa da Mata Atlântica que apresenta importância fitoquímica, fitoterapêutica, aromática e econômica, com amplo uso na medicina popular. É considerada uma espécie de difícil multiplicação via sementes, apresentando dormência por imaturidade embrionária. Assim, objetivou-se com este estudo analisar o resgate vegetativo, estabelecimento in vitro e a propagação via estaquia de Drimys brasiliensis. Para o resgate, realizou-se a aplicação das técnicas de anelamento a 30 e 90 cm do solo, semianelamento a 30 cm do solo e galhos podados acondicionados vertical e horizontalmente, sendo avaliada a capacidade de emissão de brotações. Com as brotações obtidas pelo resgate vegetativo, foram confeccionados os explantes utilizados para o estabelecimento in vitro, avaliando diferentes tempos de imersão em hipoclorito de sódio 1,0 % (v/v) (0, 10, 15 e 20 minutos). Foram avaliadas as percentagens de contaminação total, bacteriana, fúngica e oxidação fenólica. Na estaquia foram testadas brotações oriundas do resgate vegetativo, da parte aérea de indivíduo adulto e de galhos destacados e acondicionados em mini-túnel. Avaliaram-se a porcentagem de sobrevivência das estacas, porcentagem de estacas com calos e porcentagem de enraizamento. Em relação ao resgate vegetativo, a técnica de galhos podados resultou na indução de brotações, destacando os galhos acondicionados verticalmente (100%). No estabelecimento in vitro, a imersão em hipoclorito de sódio (1%) por 20 minutos se mostrou eficiente no controle da contaminação por fungos, entretanto, não foram observadas diferenças significativas em relação ao controle de contaminação bacteriana. Na estaquia, o uso de estacas provenientes de galhos podados ou anelamento/semianelamento mostrou potencial para estas técnicas na clonagem da espécie, obtendo resultados próximos a 10% de enraizamento. No geral, a espécie apresenta potencial de propagação vegetativa, contudo, são necessários ajustes nos protocolos de estabelecimento in vitro e estaquia. Universidade Federal de Santa Maria2023-04-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/6909310.5902/1980509869093Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e69093Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e690931980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/69093/52353Copyright (c) 2022 Ciência Florestalhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSilva, Jaiton Jaime das NevesNavroski, Marcio CarlosAquino, Marina Gabriela Cardoso deDenega, LucasFonseca, Pedro Henrique Tavares daOliveira, Luciana Magda dePereira, Mariane de Oliveira 2023-06-07T17:40:39Zoai:ojs.pkp.sfu.ca:article/69093Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2023-06-07T17:40:39Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers Resgate vegetativo, estabelecimento in vitro e estaquia de Drimys brasiliensis Miers |
title |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
spellingShingle |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers Silva, Jaiton Jaime das Neves Propagação vegetativa Rejuvenescimento Cultivo in vitro Espécie nativa Vegetative propagation Rejuvenation In vitro cultivation Native species |
title_short |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
title_full |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
title_fullStr |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
title_full_unstemmed |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
title_sort |
Vegetative rescue, in vitro establishment and cutting technique of Drimys brasiliensis Miers |
author |
Silva, Jaiton Jaime das Neves |
author_facet |
Silva, Jaiton Jaime das Neves Navroski, Marcio Carlos Aquino, Marina Gabriela Cardoso de Denega, Lucas Fonseca, Pedro Henrique Tavares da Oliveira, Luciana Magda de Pereira, Mariane de Oliveira |
author_role |
author |
author2 |
Navroski, Marcio Carlos Aquino, Marina Gabriela Cardoso de Denega, Lucas Fonseca, Pedro Henrique Tavares da Oliveira, Luciana Magda de Pereira, Mariane de Oliveira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Jaiton Jaime das Neves Navroski, Marcio Carlos Aquino, Marina Gabriela Cardoso de Denega, Lucas Fonseca, Pedro Henrique Tavares da Oliveira, Luciana Magda de Pereira, Mariane de Oliveira |
dc.subject.por.fl_str_mv |
Propagação vegetativa Rejuvenescimento Cultivo in vitro Espécie nativa Vegetative propagation Rejuvenation In vitro cultivation Native species |
topic |
Propagação vegetativa Rejuvenescimento Cultivo in vitro Espécie nativa Vegetative propagation Rejuvenation In vitro cultivation Native species |
description |
Drimys brasiliensis Miers, popularly known as cataia, is a tree species native to the Atlantic Forest that has phytochemical, phytotherapeutic, aromatic and economic importance, with wide use in folk medicine. It is considered a species of difficult multiplication via seeds, presenting dormancy due to embryonic immaturity. Thus, the objective of this study was to analyze the vegetative rescue, in vitro establishment and propagation by way of cuttings of Drimys brasiliensis. For the rescue, the girdling techniques were applied at 30 and 90 cm from the ground, semi-girdling at 30 cm from the ground and pruned branches conditioned vertically and horizontally, being evaluated the shoot emission capacity. With the shoots obtained by vegetative rescue, the explants used for in vitro establishment were made, evaluating different immersion times in sodium hypochlorite 1.0 % (v/v) (0, 10, 15 and 20 minutes). The percentages of total, bacterial, fungal contamination and phenolic oxidation were evaluated. On the cuttings, shoots from vegetative rescue, from the aerial part of an adult individual and from detached branches were tested and placed in a mini-tunnel. The percentage of cuttings survival, percentage of cuttings with callus and percentage of rooting were evaluated. Regarding the vegetative rescue, the technique of pruned branches resulted in the induction of shoots, highlighting the branches conditioned vertically (100%). In the in vitro establishment, the immersion in sodium hypochlorite (1%) for 20 minutes proved to be efficient in the control of fungal contamination, however, no significant differences were observed in relation to the control of bacterial contamination. In cuttings, the use of cuttings from pruned branches or girdling/semi-girdling showed potential for these techniques in the cloning of the species, obtaining results close to 10% rooting. In general, the species has the potential for vegetative propagation, however, adjustments are necessary in the in vitro establishment and cutting protocols. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/69093 10.5902/1980509869093 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/69093 |
identifier_str_mv |
10.5902/1980509869093 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/69093/52353 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e69093 Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e69093 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944136223096832 |