IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/1966 |
Resumo: | The objectives of this work were to develop an in vitro establishment protocol and to quantify growth of louro-pardo (Cordia trichotoma (Vell.) Arráb. Ex Steud.) seedlings to produce asseptic explants. Seed imbibition and disinfection were studied for in vitro seedling establishment. Seeds were imbibed in distilled and autoclaved water for 0, 24, 48, 72, 96 or 120 h. Seeds without tegument were submerged in a 70% alcohol solution for 30 s and in a hypochloride solution of 2% or 5% for 0, 5, 10, 15 or 20 min. Percentages of disinfection and germination and mean germination time were evaluated. Seedling growth of louro-pardo was quantified in 1/2MS and WPM culture mediums. Number of emerged leaves and roots and length of shoot and primary root were evaluated at 7, 14, 21 and 28 days after inoculation. Imbibition of louro-pardo seeds until 24 hours made easy tegument excision without affecting disinfection and germination. Seed-tegument excision and immersion in a 70% alcohol solution for 30 s were enough for an acceptable production of in vitro asseptic seedlings. Louro-pardo seedlings grow satisfactorily in a WPM culture medium without growth regulators. |
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IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGSEstabelecimento e crescimento in vitro de plântulas de louro-pardo.Cordia trichotomatissue culturemicropropagationCordia trichotomacultura de tecidosmicropropagaçãoThe objectives of this work were to develop an in vitro establishment protocol and to quantify growth of louro-pardo (Cordia trichotoma (Vell.) Arráb. Ex Steud.) seedlings to produce asseptic explants. Seed imbibition and disinfection were studied for in vitro seedling establishment. Seeds were imbibed in distilled and autoclaved water for 0, 24, 48, 72, 96 or 120 h. Seeds without tegument were submerged in a 70% alcohol solution for 30 s and in a hypochloride solution of 2% or 5% for 0, 5, 10, 15 or 20 min. Percentages of disinfection and germination and mean germination time were evaluated. Seedling growth of louro-pardo was quantified in 1/2MS and WPM culture mediums. Number of emerged leaves and roots and length of shoot and primary root were evaluated at 7, 14, 21 and 28 days after inoculation. Imbibition of louro-pardo seeds until 24 hours made easy tegument excision without affecting disinfection and germination. Seed-tegument excision and immersion in a 70% alcohol solution for 30 s were enough for an acceptable production of in vitro asseptic seedlings. Louro-pardo seedlings grow satisfactorily in a WPM culture medium without growth regulators.Os objetivos deste trabalho foram desenvolver um protocolo de estabelecimento e quantificar o crescimento in vitro de plântulas de louro-pardo (Cordia trichotoma (Vell.) Arráb. Ex Steud.) oriundas de sementes, para a produção de explantes assépticos. Para o estabelecimento in vitro, estudou-se o efeito do tempo de embebição e de diferentes protocolos de desinfestação das sementes. A embebiçao fez-se em água destilada e autoclavada por 0, 24, 48, 72, 96 ou 120 horas. Para a desinfestação de sementes sem tegumento, testou-se a imersão em álcool etílico 70% por 30 segundos, seguida da imersão em solução de hipoclorito de sódio a 2% ou 5% por 0, 5, 10, 15 ou 20 minutos. Foram avaliadas as percentagens de desinfestação e germinação e o tempo médio de germinação. O crescimento de explantes de louro-pardo foi quantificado nos meios de cultura 1/2MS e WPM, através do número de folhas e raízes emitidas e do comprimento da parte aérea e da raiz primária aos 7, 14, 21 e 28 dias após a inoculação. A embebição das sementes de louro-pardo por até 24 horas favoreceu a retirada do tegumento sem afetar a desinfestação e germinação. A retirada do tegumento e a imersão das sementes em solução de álcool etílico 70% por 30 segundos foram suficientes para a produção in vitro de plântulas assépticas. Plântulas de louro-pardo apresentaram melhor crescimento em meio de cultura WPM, sem a adição de reguladores de crescimento.Universidade Federal de Santa Maria2007-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/196610.5902/198050981966Ciência Florestal; Vol. 17 No. 4 (2007); 343-349Ciência Florestal; v. 17 n. 4 (2007); 343-3491980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/1966/1220Fick, Tiago AntonioBisognin, Dilson AntônioQuadros, Kenia Michele deHorbach, MicheliReiniger, Lia Rejane S.info:eu-repo/semantics/openAccess2017-05-11T19:04:57Zoai:ojs.pkp.sfu.ca:article/1966Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-05-11T19:04:57Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS Estabelecimento e crescimento in vitro de plântulas de louro-pardo. |
title |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
spellingShingle |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS Fick, Tiago Antonio Cordia trichotoma tissue culture micropropagation Cordia trichotoma cultura de tecidos micropropagação |
title_short |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
title_full |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
title_fullStr |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
title_full_unstemmed |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
title_sort |
IN VITRO ESTABLISHMENT AND GROWTH OF LOURO-PARDO (Cordia trichotoma) SEEDLINGS |
author |
Fick, Tiago Antonio |
author_facet |
Fick, Tiago Antonio Bisognin, Dilson Antônio Quadros, Kenia Michele de Horbach, Micheli Reiniger, Lia Rejane S. |
author_role |
author |
author2 |
Bisognin, Dilson Antônio Quadros, Kenia Michele de Horbach, Micheli Reiniger, Lia Rejane S. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fick, Tiago Antonio Bisognin, Dilson Antônio Quadros, Kenia Michele de Horbach, Micheli Reiniger, Lia Rejane S. |
dc.subject.por.fl_str_mv |
Cordia trichotoma tissue culture micropropagation Cordia trichotoma cultura de tecidos micropropagação |
topic |
Cordia trichotoma tissue culture micropropagation Cordia trichotoma cultura de tecidos micropropagação |
description |
The objectives of this work were to develop an in vitro establishment protocol and to quantify growth of louro-pardo (Cordia trichotoma (Vell.) Arráb. Ex Steud.) seedlings to produce asseptic explants. Seed imbibition and disinfection were studied for in vitro seedling establishment. Seeds were imbibed in distilled and autoclaved water for 0, 24, 48, 72, 96 or 120 h. Seeds without tegument were submerged in a 70% alcohol solution for 30 s and in a hypochloride solution of 2% or 5% for 0, 5, 10, 15 or 20 min. Percentages of disinfection and germination and mean germination time were evaluated. Seedling growth of louro-pardo was quantified in 1/2MS and WPM culture mediums. Number of emerged leaves and roots and length of shoot and primary root were evaluated at 7, 14, 21 and 28 days after inoculation. Imbibition of louro-pardo seeds until 24 hours made easy tegument excision without affecting disinfection and germination. Seed-tegument excision and immersion in a 70% alcohol solution for 30 s were enough for an acceptable production of in vitro asseptic seedlings. Louro-pardo seedlings grow satisfactorily in a WPM culture medium without growth regulators. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/1966 10.5902/198050981966 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/1966 |
identifier_str_mv |
10.5902/198050981966 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/1966/1220 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 17 No. 4 (2007); 343-349 Ciência Florestal; v. 17 n. 4 (2007); 343-349 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944127149768704 |