DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD

Detalhes bibliográficos
Autor(a) principal: Martins-Corder, Maisa Pimentel
Data de Publicação: 1999
Outros Autores: Borges Junior, Norton
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/380
Resumo: Seed dormancy and the presence microrganism  are important factors that can reduce the germinative vigorousness  of seeds of Acacia mearnsii. The presence of fungi and bacteria in  the seeds used in laboratory tests can infest explantes used in  micropropagation. The objetives of the present study were to determine a methodology  the   break tho dormency   of the seeds of the Acacia mearnsii, and  desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The  control was treated with hot water (800C) for three minutes with: Benlate  at  6%,  hypochloride  sodium  at  10%  and  or  alcohol   at  70%. The  assay  was conducted in an incubation room at  250C (±3) and 12 hours with fluorescent light. Autoclaving  of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed  the embryo.
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spelling DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILDDesinfestação e quebra de dormência de sementes de Acacia mearnsii De Wild.dormancy breakdesinfestionseedsAcacia.quebra de dormênciadesinfestaçãosementesAcacia..Seed dormancy and the presence microrganism  are important factors that can reduce the germinative vigorousness  of seeds of Acacia mearnsii. The presence of fungi and bacteria in  the seeds used in laboratory tests can infest explantes used in  micropropagation. The objetives of the present study were to determine a methodology  the   break tho dormency   of the seeds of the Acacia mearnsii, and  desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The  control was treated with hot water (800C) for three minutes with: Benlate  at  6%,  hypochloride  sodium  at  10%  and  or  alcohol   at  70%. The  assay  was conducted in an incubation room at  250C (±3) and 12 hours with fluorescent light. Autoclaving  of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed  the embryo. A dormência e a presença de microrganismos são importantes fatores que podem reduzir o vigor germinativo de sementes de Acacia mearnsii.  A presença de fungos e bactérias junto às sementes empregadas em testes de laboratório podem fornecer explantes contaminados quando utilizados nas culturas in vitro.  Assim, os objetivos do presente estudo foram determinar uma metodologia adequada para a quebra de dormência de sementes da acácia-negra e indicar um método eficiente para desinfestação das sementes. Sementes de acácia negra foram autoclavadas durante diferentes períodos: 5, 10, 15, 20, 25 e 30 minutos. As  testemunhas usadas sofreram quebra de dormência com água quente (800C) por três minutos, e foram tratadas com:  fungicida Benomyl,  produto comercial Hipoclorito de sodio e/ou  álcool a 70%.  O ensaio foi conduzido em sala de incubação com temperatura de 250C (± 3) e fotoperíodo de 12 horas de luz fluorescente. Os resultados mostraram que a autoclavagem das sementes durante 20 e 25 minutos foram suficientes para quebrar a dormência e simultaneamente, promover a desinfestação de sementes de acácia-negra. Períodos de autoclavagem inferiores a 20 minutos, não foram eficientes na desinfestação de sementes de acácia-negra, embora tivessem propiciado elevados percentuais de germinação. A exposição de sementes à autoclavagem, por 30 minutos, promoveu a desinfestação das sementes, porém levou o embrião à morte.Universidade Federal de Santa Maria1999-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/38010.5902/19805098380Ciência Florestal; Vol. 9 No. 2 (1999); 1-7Ciência Florestal; v. 9 n. 2 (1999); 1-71980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/380/252Martins-Corder, Maisa PimentelBorges Junior, Nortoninfo:eu-repo/semantics/openAccess2017-06-07T19:14:42Zoai:ojs.pkp.sfu.ca:article/380Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-06-07T19:14:42Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
Desinfestação e quebra de dormência de sementes de Acacia mearnsii De Wild.
title DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
spellingShingle DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
Martins-Corder, Maisa Pimentel
dormancy break
desinfestion
seeds
Acacia.
quebra de dormência
desinfestação
sementes
Acacia..
title_short DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
title_full DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
title_fullStr DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
title_full_unstemmed DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
title_sort DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
author Martins-Corder, Maisa Pimentel
author_facet Martins-Corder, Maisa Pimentel
Borges Junior, Norton
author_role author
author2 Borges Junior, Norton
author2_role author
dc.contributor.author.fl_str_mv Martins-Corder, Maisa Pimentel
Borges Junior, Norton
dc.subject.por.fl_str_mv dormancy break
desinfestion
seeds
Acacia.
quebra de dormência
desinfestação
sementes
Acacia..
topic dormancy break
desinfestion
seeds
Acacia.
quebra de dormência
desinfestação
sementes
Acacia..
description Seed dormancy and the presence microrganism  are important factors that can reduce the germinative vigorousness  of seeds of Acacia mearnsii. The presence of fungi and bacteria in  the seeds used in laboratory tests can infest explantes used in  micropropagation. The objetives of the present study were to determine a methodology  the   break tho dormency   of the seeds of the Acacia mearnsii, and  desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The  control was treated with hot water (800C) for three minutes with: Benlate  at  6%,  hypochloride  sodium  at  10%  and  or  alcohol   at  70%. The  assay  was conducted in an incubation room at  250C (±3) and 12 hours with fluorescent light. Autoclaving  of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed  the embryo.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/380
10.5902/19805098380
url https://periodicos.ufsm.br/cienciaflorestal/article/view/380
identifier_str_mv 10.5902/19805098380
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/380/252
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 9 No. 2 (1999); 1-7
Ciência Florestal; v. 9 n. 2 (1999); 1-7
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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