DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/380 |
Resumo: | Seed dormancy and the presence microrganism are important factors that can reduce the germinative vigorousness of seeds of Acacia mearnsii. The presence of fungi and bacteria in the seeds used in laboratory tests can infest explantes used in micropropagation. The objetives of the present study were to determine a methodology the break tho dormency of the seeds of the Acacia mearnsii, and desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The control was treated with hot water (800C) for three minutes with: Benlate at 6%, hypochloride sodium at 10% and or alcohol at 70%. The assay was conducted in an incubation room at 250C (±3) and 12 hours with fluorescent light. Autoclaving of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed the embryo. |
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Ciência Florestal (Online) |
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DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILDDesinfestação e quebra de dormência de sementes de Acacia mearnsii De Wild.dormancy breakdesinfestionseedsAcacia.quebra de dormênciadesinfestaçãosementesAcacia..Seed dormancy and the presence microrganism are important factors that can reduce the germinative vigorousness of seeds of Acacia mearnsii. The presence of fungi and bacteria in the seeds used in laboratory tests can infest explantes used in micropropagation. The objetives of the present study were to determine a methodology the break tho dormency of the seeds of the Acacia mearnsii, and desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The control was treated with hot water (800C) for three minutes with: Benlate at 6%, hypochloride sodium at 10% and or alcohol at 70%. The assay was conducted in an incubation room at 250C (±3) and 12 hours with fluorescent light. Autoclaving of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed the embryo. A dormência e a presença de microrganismos são importantes fatores que podem reduzir o vigor germinativo de sementes de Acacia mearnsii. A presença de fungos e bactérias junto às sementes empregadas em testes de laboratório podem fornecer explantes contaminados quando utilizados nas culturas in vitro. Assim, os objetivos do presente estudo foram determinar uma metodologia adequada para a quebra de dormência de sementes da acácia-negra e indicar um método eficiente para desinfestação das sementes. Sementes de acácia negra foram autoclavadas durante diferentes períodos: 5, 10, 15, 20, 25 e 30 minutos. As testemunhas usadas sofreram quebra de dormência com água quente (800C) por três minutos, e foram tratadas com: fungicida Benomyl, produto comercial Hipoclorito de sodio e/ou álcool a 70%. O ensaio foi conduzido em sala de incubação com temperatura de 250C (± 3) e fotoperíodo de 12 horas de luz fluorescente. Os resultados mostraram que a autoclavagem das sementes durante 20 e 25 minutos foram suficientes para quebrar a dormência e simultaneamente, promover a desinfestação de sementes de acácia-negra. Períodos de autoclavagem inferiores a 20 minutos, não foram eficientes na desinfestação de sementes de acácia-negra, embora tivessem propiciado elevados percentuais de germinação. A exposição de sementes à autoclavagem, por 30 minutos, promoveu a desinfestação das sementes, porém levou o embrião à morte.Universidade Federal de Santa Maria1999-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/38010.5902/19805098380Ciência Florestal; Vol. 9 No. 2 (1999); 1-7Ciência Florestal; v. 9 n. 2 (1999); 1-71980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/380/252Martins-Corder, Maisa PimentelBorges Junior, Nortoninfo:eu-repo/semantics/openAccess2017-06-07T19:14:42Zoai:ojs.pkp.sfu.ca:article/380Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-06-07T19:14:42Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD Desinfestação e quebra de dormência de sementes de Acacia mearnsii De Wild. |
title |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
spellingShingle |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD Martins-Corder, Maisa Pimentel dormancy break desinfestion seeds Acacia. quebra de dormência desinfestação sementes Acacia.. |
title_short |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
title_full |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
title_fullStr |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
title_full_unstemmed |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
title_sort |
DESINFESTATION AND BREAK OF DORMANCY OF SEEDS OF Acacia mearnsii DE WILD |
author |
Martins-Corder, Maisa Pimentel |
author_facet |
Martins-Corder, Maisa Pimentel Borges Junior, Norton |
author_role |
author |
author2 |
Borges Junior, Norton |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Martins-Corder, Maisa Pimentel Borges Junior, Norton |
dc.subject.por.fl_str_mv |
dormancy break desinfestion seeds Acacia. quebra de dormência desinfestação sementes Acacia.. |
topic |
dormancy break desinfestion seeds Acacia. quebra de dormência desinfestação sementes Acacia.. |
description |
Seed dormancy and the presence microrganism are important factors that can reduce the germinative vigorousness of seeds of Acacia mearnsii. The presence of fungi and bacteria in the seeds used in laboratory tests can infest explantes used in micropropagation. The objetives of the present study were to determine a methodology the break tho dormency of the seeds of the Acacia mearnsii, and desinfest the seeds. Seeds of Acacia mearnsii were autoclaved for: 5, 10, 15, 20, 25 or 30 minutes. The control was treated with hot water (800C) for three minutes with: Benlate at 6%, hypochloride sodium at 10% and or alcohol at 70%. The assay was conducted in an incubation room at 250C (±3) and 12 hours with fluorescent light. Autoclaving of the seeds for 20 and 25 minutes were enough to break the seeds dormancy and simultaneously, to promote the black-wattle desinfestation. Periods inferior to 20 minutes were not efficient in the desinfestation of the seeds, although they have promoted high rote of seeds germination. The seeds exposition to the autoclaving for 30 minutes has promoted the seeds desinfestation, but it also killed the embryo. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/380 10.5902/19805098380 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/380 |
identifier_str_mv |
10.5902/19805098380 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/380/252 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 9 No. 2 (1999); 1-7 Ciência Florestal; v. 9 n. 2 (1999); 1-7 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944124892184576 |