MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.

Detalhes bibliográficos
Autor(a) principal: Mantovani, Nilton César
Data de Publicação: 1999
Outros Autores: Franco, Elci Terezinha Henz, Guerra, Miguel P., Hoppe, Juarez Martins
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
DOI: 10.5902/19805098365
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/365
Resumo: This study was carried out aiming to study the in vitro regeneration of caixeta (Didymopanax morototoni (Aubl.) Dcne. et Planch.). In order to establish a basic micropropagation protocol axillary buds and nodal segments were desinfested with sodium hipochlorite 10 % and innoculated in two basal media: WPM (Lloyd & McCown, 1981) and MS (Murashige & Skoog, 1962) supplemented with BAP, TDZ, NAA, GA3, IBA and activated charcoal in defferent combinations and concentrations for each developmental step. The desinfestation method based on sodium hipochlorite during 10 minutes allowed the best results for the establishment of aseptic cultures in nodal segments. With these explants the regeneration rate and the shoot elongation were higher in the WPM medium containing 1,0 mg.l-1 BAP. However after three subcultures it was observed a reduction in the regeneration rate. The  rooting   of  regenerated  shoots  was  observed  in  a   WPM medium supplemented with 1,0 mg.l-1 IBA. The acclimatization process allowed  the plantlet survival in ex vitro conditions.
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spelling MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.Micropropagação de caixeta, Didymopanax morototoni (Aubl.) Dcne. et Planch.Didymopanax morototonimicropropagationPlant tissue culture.Didymopanax morototonimicropropagaçãocultura de tecidos vegetais.This study was carried out aiming to study the in vitro regeneration of caixeta (Didymopanax morototoni (Aubl.) Dcne. et Planch.). In order to establish a basic micropropagation protocol axillary buds and nodal segments were desinfested with sodium hipochlorite 10 % and innoculated in two basal media: WPM (Lloyd & McCown, 1981) and MS (Murashige & Skoog, 1962) supplemented with BAP, TDZ, NAA, GA3, IBA and activated charcoal in defferent combinations and concentrations for each developmental step. The desinfestation method based on sodium hipochlorite during 10 minutes allowed the best results for the establishment of aseptic cultures in nodal segments. With these explants the regeneration rate and the shoot elongation were higher in the WPM medium containing 1,0 mg.l-1 BAP. However after three subcultures it was observed a reduction in the regeneration rate. The  rooting   of  regenerated  shoots  was  observed  in  a   WPM medium supplemented with 1,0 mg.l-1 IBA. The acclimatization process allowed  the plantlet survival in ex vitro conditions. O presente trabalho objetivou estabelecer um protocolo básico para a micropropagação da caixeta Didymopanax morototoni (Aubl.) Dcne. et Planch. Segmentos nodais foram desinfestados em hipoclorito de sódio 10 % por diferentes periodos de exposição, e inoculados em dois meios básicos: WPM (Lloyd & McCown, 1981) e MS (Murashige & Skoog, 1962) suplementados com BAP, TDZ, ANA, GA3 , AIB e carvão ativado em diferentes combinações e concentrações para cada estádio de desenvolvimento. A desinfestação dos segmentos nodais com hipoclorito de sódio por 10 minutos proporcionou os melhores resultados para o estabelecimento asséptico das culturas. A maior taxa de regeneração e alongamento de brotações foi obtida com o meio WPM contendo 1,0 mg.l-1 de BAP. Contudo após três subcultivos neste mesmo meio observou-se redução na taxa de multiplicação. O enraizamento das brotações foi obtido no meio WPM suplementado com 1,0 mg.l-1 de AIB. O processo de aclimatização possibilitou a sobrevivência das plântulas micropropagadas às condições ex vitro.Universidade Federal de Santa Maria1999-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/36510.5902/19805098365Ciência Florestal; Vol. 9 No. 1 (1999); 47-61Ciência Florestal; v. 9 n. 1 (1999); 47-611980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/365/235Mantovani, Nilton CésarFranco, Elci Terezinha HenzGuerra, Miguel P.Hoppe, Juarez Martinsinfo:eu-repo/semantics/openAccess2017-06-08T14:17:28Zoai:ojs.pkp.sfu.ca:article/365Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-06-08T14:17:28Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
Micropropagação de caixeta, Didymopanax morototoni (Aubl.) Dcne. et Planch.
title MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
spellingShingle MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
Mantovani, Nilton César
Didymopanax morototoni
micropropagation
Plant tissue culture.
Didymopanax morototoni
micropropagação
cultura de tecidos vegetais.
Mantovani, Nilton César
Didymopanax morototoni
micropropagation
Plant tissue culture.
Didymopanax morototoni
micropropagação
cultura de tecidos vegetais.
title_short MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
title_full MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
title_fullStr MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
title_full_unstemmed MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
title_sort MICROPROPAGATION OF CAIXETA Didymopanax morototoni (Aubl.) Dcne. et Planch.
author Mantovani, Nilton César
author_facet Mantovani, Nilton César
Mantovani, Nilton César
Franco, Elci Terezinha Henz
Guerra, Miguel P.
Hoppe, Juarez Martins
Franco, Elci Terezinha Henz
Guerra, Miguel P.
Hoppe, Juarez Martins
author_role author
author2 Franco, Elci Terezinha Henz
Guerra, Miguel P.
Hoppe, Juarez Martins
author2_role author
author
author
dc.contributor.author.fl_str_mv Mantovani, Nilton César
Franco, Elci Terezinha Henz
Guerra, Miguel P.
Hoppe, Juarez Martins
dc.subject.por.fl_str_mv Didymopanax morototoni
micropropagation
Plant tissue culture.
Didymopanax morototoni
micropropagação
cultura de tecidos vegetais.
topic Didymopanax morototoni
micropropagation
Plant tissue culture.
Didymopanax morototoni
micropropagação
cultura de tecidos vegetais.
description This study was carried out aiming to study the in vitro regeneration of caixeta (Didymopanax morototoni (Aubl.) Dcne. et Planch.). In order to establish a basic micropropagation protocol axillary buds and nodal segments were desinfested with sodium hipochlorite 10 % and innoculated in two basal media: WPM (Lloyd & McCown, 1981) and MS (Murashige & Skoog, 1962) supplemented with BAP, TDZ, NAA, GA3, IBA and activated charcoal in defferent combinations and concentrations for each developmental step. The desinfestation method based on sodium hipochlorite during 10 minutes allowed the best results for the establishment of aseptic cultures in nodal segments. With these explants the regeneration rate and the shoot elongation were higher in the WPM medium containing 1,0 mg.l-1 BAP. However after three subcultures it was observed a reduction in the regeneration rate. The  rooting   of  regenerated  shoots  was  observed  in  a   WPM medium supplemented with 1,0 mg.l-1 IBA. The acclimatization process allowed  the plantlet survival in ex vitro conditions.
publishDate 1999
dc.date.none.fl_str_mv 1999-03-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/365
10.5902/19805098365
url https://periodicos.ufsm.br/cienciaflorestal/article/view/365
identifier_str_mv 10.5902/19805098365
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/365/235
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 9 No. 1 (1999); 47-61
Ciência Florestal; v. 9 n. 1 (1999); 47-61
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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dc.identifier.doi.none.fl_str_mv 10.5902/19805098365

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