Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax

Detalhes bibliográficos
Autor(a) principal: Kuhn, Paulo Roberto
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/4944
Resumo: Had it aimed in this study to survey the plant-parasitic nematodes in the Serra Gaúcha vineyards with symptoms of decline; evaluate the pathogenicity and aggressiveness Mesocriconema xenoplax in vine and peach; and test the susceptibility of weed and forage the two population M. xenoplax.First we evaluated the diversity of genres and populcional density of plant-parasitic nematode genera associated to nine vineyards of the Serra Gaúcha.In the survey the Mesocriconema genera were identified; Ogma; Meloidogyne; Paratrichodorus; Helicotylenchus; Xiphinema; Pratylenchus; Hemicicliophora and free-living nematodes, and the free living nematodes and Mesocrinema apresentram in more densely populated than others.The following was characterized biochemically by electrophoresis populations and morphological and morphometric Meloidogine three populations of ringed nematode Mesocriconema genre with the highest population levels, eight esterásticas bands were identified and four phenotypes of esterase (Est) which corresponded to the species M. javanica Est J3, M. incognita Est I2, M. arenaria and M. hapla A2 Est Est H1. the only species identified the Mesocriconema gender was Mesocriconema xenoplax.Subsequently, three cultivars room (Concord, Niagara pink, Claret) and a rootstock (Paulsen) vine and the cultivar Peach Capdbosq were inoculated or not with 2000 M. xenoplax specimens from four different populations, coming from three vineyards Serra Gaúcha and a coming of peach orchard in the city of Pelotas, Brazil, kept in the collection by Embrapa Clima Temperado.Every 30 days was determined chlorophyll content of plants. After 180 inoculation, the plants of each treatment were measured morphologically and analyzed for length of the main branch, fresh weight of aerial part, stem diameter, main root length, fresh weight of root and leaf area. Also enzymatic activity was determined peroxidase branches of each plant.Then the soil in each sample was processed for determination of the final population and calculating the reproduction factor of each population of M. xenoplax each cultivar to evaluate the pathogenicity of the nematode and aggressiveness.Among the populations of M. xenoplax tested, it was observed that there is a difference as to the aggressiveness characterizing the population 2 of from vine to be the most aggressive, which is pathogenic to all genotypes of grapevine, the board was cultivating the most susceptible.In the evaluation of forage species to populations d M. xenoplax tested, vetch, sunn hemp, bermudagrass and grass evergreen, provided higher FR M. xenoplax, being considered as susceptible host plants and vine groves and peach with a history of presence of M. xenoplax must be eliminated. Unlike grasses oat; oats; ryegrass; millet and sorghum can be used as cover crops in orchards and vine peach contaminated with Mesocriconema xenoplax due to its low reproducibility in these plants.
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spelling 2015-11-092015-11-092015-03-07KUHN, Paulo Roberto. Nematofauna diversity in vine groves with symptoms of decline and aggression of Mesocriconema xenoplax. 2015. 103 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/4944Had it aimed in this study to survey the plant-parasitic nematodes in the Serra Gaúcha vineyards with symptoms of decline; evaluate the pathogenicity and aggressiveness Mesocriconema xenoplax in vine and peach; and test the susceptibility of weed and forage the two population M. xenoplax.First we evaluated the diversity of genres and populcional density of plant-parasitic nematode genera associated to nine vineyards of the Serra Gaúcha.In the survey the Mesocriconema genera were identified; Ogma; Meloidogyne; Paratrichodorus; Helicotylenchus; Xiphinema; Pratylenchus; Hemicicliophora and free-living nematodes, and the free living nematodes and Mesocrinema apresentram in more densely populated than others.The following was characterized biochemically by electrophoresis populations and morphological and morphometric Meloidogine three populations of ringed nematode Mesocriconema genre with the highest population levels, eight esterásticas bands were identified and four phenotypes of esterase (Est) which corresponded to the species M. javanica Est J3, M. incognita Est I2, M. arenaria and M. hapla A2 Est Est H1. the only species identified the Mesocriconema gender was Mesocriconema xenoplax.Subsequently, three cultivars room (Concord, Niagara pink, Claret) and a rootstock (Paulsen) vine and the cultivar Peach Capdbosq were inoculated or not with 2000 M. xenoplax specimens from four different populations, coming from three vineyards Serra Gaúcha and a coming of peach orchard in the city of Pelotas, Brazil, kept in the collection by Embrapa Clima Temperado.Every 30 days was determined chlorophyll content of plants. After 180 inoculation, the plants of each treatment were measured morphologically and analyzed for length of the main branch, fresh weight of aerial part, stem diameter, main root length, fresh weight of root and leaf area. Also enzymatic activity was determined peroxidase branches of each plant.Then the soil in each sample was processed for determination of the final population and calculating the reproduction factor of each population of M. xenoplax each cultivar to evaluate the pathogenicity of the nematode and aggressiveness.Among the populations of M. xenoplax tested, it was observed that there is a difference as to the aggressiveness characterizing the population 2 of from vine to be the most aggressive, which is pathogenic to all genotypes of grapevine, the board was cultivating the most susceptible.In the evaluation of forage species to populations d M. xenoplax tested, vetch, sunn hemp, bermudagrass and grass evergreen, provided higher FR M. xenoplax, being considered as susceptible host plants and vine groves and peach with a history of presence of M. xenoplax must be eliminated. Unlike grasses oat; oats; ryegrass; millet and sorghum can be used as cover crops in orchards and vine peach contaminated with Mesocriconema xenoplax due to its low reproducibility in these plants.Teve-se por objetivo neste estudo realizar um levantamento da nematofauna em pomares de videira da Serra Gaúcha que apresentavam sintomas de declínio; avaliar a patogenicidade e agressividade do nematoide anelado Mesocriconema xenoplax em videira e pessegueiro; e testar a suscetibilidade de plantas daninhas e forrageiras a duas populações de M. xenoplax. O estudo da diversidade e densidade populacional de nematoides foi realizado nove pomares de videira da Serra Gaúcha, onde foram identificados os gêneros Mesocriconema; Ogma; Meloidogyne; Paratrichodorus; Helicotylenchus; Xiphinema; Pratylenchus; Hemicicliophora e nematoides de vida-livre, sendo que os nematoides de vida livre e Mesocriconema apresentaram-se em maior densidade populacional que os demais. Caracterizou-se bioquimicamente pela técnica de eletroforese as populações de Meloidogyne e morfometricamente três populações do gênero Mesocriconema. Para Meloidogyne foram identificados oito bandas esterásticas e quatro fenótipos de esterase (Est) os quais corresponderam às espécies Meloidogyne javanica Est J3, M. incognita Est I2, M. arenaria Est A2 e M. hapla Est H1 e identificada apenas uma espécie, M. xenoplax. Posteriormente, três cultivares copa (Concord, Niagara rosada, Bordô) e um porta-enxerto (Paulsen) de videira e a cultivar de pessegueiro Capdbosq foram inoculadascom 2000 espécimes de M. xenoplax de quatro populações distintas, três oriundas de pomares de videira da Serra Gaúcha e uma oriunda de pomar de pessegueiro do município de Pelotas, RS. Após a instação do insaio, a cada 30 dias foi determinado o teor de clorofila das plantas, e aos 180 dias as plantas foram analisadas quanto ao comprimento do ramo principal, massa fresca da parte aérea, diâmetro do colo, comprimento da raiz principal, massa fresca do sistema radicular e área foliar. Também foi determinada a atividade enzimática de peroxidase dos ramos de cada planta. A seguir, foram processados os solos e determinado o fator de reprodução de cada população de M. xenoplax para avaliação da patogenicidade e agressividade do nematoide. Entre as populações de M. xenoplax testadas, observou-se que existe diferença quanto à agressividade, caracterizando-se a população 2 proveniente de videira como a mais agressiva, sendo esta patogênica a todos genótipos testados, cuja cultivar Bordô foi a mais suscetível. Na avaliação das espécies forrageiras as populações de M. xenoplax testadas, ervilhaca, crotalária, grama-seda e grama sempre-verde, proporcionaram maior FR, sendo consideradas como plantas hospedeiras suscetíveis em pomares de videira e pessegueiro com histórico da presença de M. xenoplax. As gramíneas aveia branca; aveia preta; azevém; milheto e sorgo, foram consideradoas como más hospedeiras a Mesocriconema xenoplax.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Agronomia - Agricultura e AmbienteUFSMBRAgronomiaNematoide aneladoCultivaresSuscetibilidadeRing nematodeCultivarsSusceptibilityCNPQ::CIENCIAS AGRARIAS::AGRONOMIADiversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplaxNematofauna diversity in vine groves with symptoms of decline and aggression of Mesocriconema xenoplaxinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisKulczynski, Stela Marishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782809H1Gomes, Cesar Bauerhttp://lattes.cnpq.br/3411662870994341Trevisan, Renatohttp://lattes.cnpq.br/4149730739593516Muniz, Marlove Fátima Briãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785175A9http://lattes.cnpq.br/1312094898634538Kuhn, Paulo Roberto5001000000094003003003003005004ab3724d-5b5c-46a3-a79a-dace0b8313a448781b33-b3ef-47c1-a3ca-a61fd75a565ca0e52409-8590-49ff-bcb7-b7c82a003b9303ebcf10-7c11-478b-a6d7-e7d506c5491efac7d9a2-a25b-42d4-98a7-0bb1c27df1efinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALKUHN, PAULO ROBERTO.pdfapplication/pdf1552419http://repositorio.ufsm.br/bitstream/1/4944/1/KUHN%2c%20PAULO%20ROBERTO.pdfb0b9b0802b0c64643cfbb8376f004990MD51TEXTKUHN, PAULO ROBERTO.pdf.txtKUHN, PAULO ROBERTO.pdf.txtExtracted texttext/plain196923http://repositorio.ufsm.br/bitstream/1/4944/2/KUHN%2c%20PAULO%20ROBERTO.pdf.txtdfb6439ee79dbddc4210f76fb01efde0MD52THUMBNAILKUHN, PAULO ROBERTO.pdf.jpgKUHN, PAULO ROBERTO.pdf.jpgIM Thumbnailimage/jpeg5112http://repositorio.ufsm.br/bitstream/1/4944/3/KUHN%2c%20PAULO%20ROBERTO.pdf.jpg02014ef09249dee3de13fc3951f51c6cMD531/49442022-03-09 16:25:58.91oai:repositorio.ufsm.br:1/4944Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-03-09T19:25:58Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
dc.title.alternative.eng.fl_str_mv Nematofauna diversity in vine groves with symptoms of decline and aggression of Mesocriconema xenoplax
title Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
spellingShingle Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
Kuhn, Paulo Roberto
Nematoide anelado
Cultivares
Suscetibilidade
Ring nematode
Cultivars
Susceptibility
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
title_short Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
title_full Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
title_fullStr Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
title_full_unstemmed Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
title_sort Diversidade da nematofauna em pomares de videira com sintomas de declínio e agressividade de Mesocriconema xenoplax
author Kuhn, Paulo Roberto
author_facet Kuhn, Paulo Roberto
author_role author
dc.contributor.advisor1.fl_str_mv Kulczynski, Stela Maris
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782809H1
dc.contributor.advisor-co1.fl_str_mv Gomes, Cesar Bauer
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/3411662870994341
dc.contributor.referee1.fl_str_mv Trevisan, Renato
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4149730739593516
dc.contributor.referee2.fl_str_mv Muniz, Marlove Fátima Brião
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785175A9
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1312094898634538
dc.contributor.author.fl_str_mv Kuhn, Paulo Roberto
contributor_str_mv Kulczynski, Stela Maris
Gomes, Cesar Bauer
Trevisan, Renato
Muniz, Marlove Fátima Brião
dc.subject.por.fl_str_mv Nematoide anelado
Cultivares
Suscetibilidade
topic Nematoide anelado
Cultivares
Suscetibilidade
Ring nematode
Cultivars
Susceptibility
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
dc.subject.eng.fl_str_mv Ring nematode
Cultivars
Susceptibility
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
description Had it aimed in this study to survey the plant-parasitic nematodes in the Serra Gaúcha vineyards with symptoms of decline; evaluate the pathogenicity and aggressiveness Mesocriconema xenoplax in vine and peach; and test the susceptibility of weed and forage the two population M. xenoplax.First we evaluated the diversity of genres and populcional density of plant-parasitic nematode genera associated to nine vineyards of the Serra Gaúcha.In the survey the Mesocriconema genera were identified; Ogma; Meloidogyne; Paratrichodorus; Helicotylenchus; Xiphinema; Pratylenchus; Hemicicliophora and free-living nematodes, and the free living nematodes and Mesocrinema apresentram in more densely populated than others.The following was characterized biochemically by electrophoresis populations and morphological and morphometric Meloidogine three populations of ringed nematode Mesocriconema genre with the highest population levels, eight esterásticas bands were identified and four phenotypes of esterase (Est) which corresponded to the species M. javanica Est J3, M. incognita Est I2, M. arenaria and M. hapla A2 Est Est H1. the only species identified the Mesocriconema gender was Mesocriconema xenoplax.Subsequently, three cultivars room (Concord, Niagara pink, Claret) and a rootstock (Paulsen) vine and the cultivar Peach Capdbosq were inoculated or not with 2000 M. xenoplax specimens from four different populations, coming from three vineyards Serra Gaúcha and a coming of peach orchard in the city of Pelotas, Brazil, kept in the collection by Embrapa Clima Temperado.Every 30 days was determined chlorophyll content of plants. After 180 inoculation, the plants of each treatment were measured morphologically and analyzed for length of the main branch, fresh weight of aerial part, stem diameter, main root length, fresh weight of root and leaf area. Also enzymatic activity was determined peroxidase branches of each plant.Then the soil in each sample was processed for determination of the final population and calculating the reproduction factor of each population of M. xenoplax each cultivar to evaluate the pathogenicity of the nematode and aggressiveness.Among the populations of M. xenoplax tested, it was observed that there is a difference as to the aggressiveness characterizing the population 2 of from vine to be the most aggressive, which is pathogenic to all genotypes of grapevine, the board was cultivating the most susceptible.In the evaluation of forage species to populations d M. xenoplax tested, vetch, sunn hemp, bermudagrass and grass evergreen, provided higher FR M. xenoplax, being considered as susceptible host plants and vine groves and peach with a history of presence of M. xenoplax must be eliminated. Unlike grasses oat; oats; ryegrass; millet and sorghum can be used as cover crops in orchards and vine peach contaminated with Mesocriconema xenoplax due to its low reproducibility in these plants.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-11-09
dc.date.available.fl_str_mv 2015-11-09
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dc.identifier.citation.fl_str_mv KUHN, Paulo Roberto. Nematofauna diversity in vine groves with symptoms of decline and aggression of Mesocriconema xenoplax. 2015. 103 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/4944
identifier_str_mv KUHN, Paulo Roberto. Nematofauna diversity in vine groves with symptoms of decline and aggression of Mesocriconema xenoplax. 2015. 103 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.
url http://repositorio.ufsm.br/handle/1/4944
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