Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/14609 |
Resumo: | In the last decade, among the medium-sized herds (poultry, swine, sheep), the one with the highest growth rate was sheep. For the sheep farming, gastrointestinal helminths can be considered one of the main barriers for breeding today. Among these, we highlight the haemoncosis, caused by the Haemonchus contortus species, a cosmopolitan parasite, highly pathogenic and prevalent. This disease, in addition to causing the death and delayed development of animals, reduces production, resulting in increased breeding costs. Due to the importance of the control of this parasitosis, the massive and indiscriminate use of antihelmintics promoted the selection of multiresistant nematodes and, consequently, the deficient action of the products commercially available. Thus, to research this topic is essential for the prevention and control of infections caused by H. contortus. In this context, this thesis was elaborated with the aim of evaluate different experimental protocols for the establishment of H. contortus infection of Meriones unguiculatus with and without immunosuppression (manuscript 1); to produce and characterize anti-H. contortus immunoglobulins Y (IgY) from third instar larvae (L3) (manuscript 2) and adult stages of this helminth (manuscript 3), and to evaluate the IgY immunotherapeutic activity anti-H. contortus in Meriones unguiculatus experimentally infected. We observed that is possible to establish gerbils’ infection by H. contortus, after immunosuppression with methylprednisolone compared to dexamethasone immunosuppression and immunocompetent animals. Chickens immunized with third-stage larvae and adults of H. contortus produced immunoglobulins anti-H. contortus displaying specificity and high avidity. Lastly, we verified that immunotherapy interfered in the establishment of H. contortus in experimentally infected gerbils. |
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2018-10-19T17:41:07Z2018-10-19T17:41:07Z2018-02-23http://repositorio.ufsm.br/handle/1/14609In the last decade, among the medium-sized herds (poultry, swine, sheep), the one with the highest growth rate was sheep. For the sheep farming, gastrointestinal helminths can be considered one of the main barriers for breeding today. Among these, we highlight the haemoncosis, caused by the Haemonchus contortus species, a cosmopolitan parasite, highly pathogenic and prevalent. This disease, in addition to causing the death and delayed development of animals, reduces production, resulting in increased breeding costs. Due to the importance of the control of this parasitosis, the massive and indiscriminate use of antihelmintics promoted the selection of multiresistant nematodes and, consequently, the deficient action of the products commercially available. Thus, to research this topic is essential for the prevention and control of infections caused by H. contortus. In this context, this thesis was elaborated with the aim of evaluate different experimental protocols for the establishment of H. contortus infection of Meriones unguiculatus with and without immunosuppression (manuscript 1); to produce and characterize anti-H. contortus immunoglobulins Y (IgY) from third instar larvae (L3) (manuscript 2) and adult stages of this helminth (manuscript 3), and to evaluate the IgY immunotherapeutic activity anti-H. contortus in Meriones unguiculatus experimentally infected. We observed that is possible to establish gerbils’ infection by H. contortus, after immunosuppression with methylprednisolone compared to dexamethasone immunosuppression and immunocompetent animals. Chickens immunized with third-stage larvae and adults of H. contortus produced immunoglobulins anti-H. contortus displaying specificity and high avidity. Lastly, we verified that immunotherapy interfered in the establishment of H. contortus in experimentally infected gerbils.Na última década, dentre os rebanhos de médio porte (aves, suínos, ovinos), o que apresentou o maior índice de crescimento foi o efetivo ovino. Para a ovinocultura, as helmintoses gastrintestinais podem ser consideradas um dos principais entraves para criação atualmente. Dentre essas, destacamos a haemoncose, causada pela espécie Haemonchus contortus, parasito cosmopolita, altamente patogênico e prevalente. Esta enfermidade, além de causar a morte de animais e retardo no desenvolvimento, reduz a produção, gerando maiores custos e maior tempo para o retorno do capital empregado. Devido à importância do controle desta parasitose, o uso massivo e indiscriminado de anti-helmínticos culminou com seleção de nematódeos multirresistentes e por consequência, a deficiente ação dos produtos disponíveis no mercado. Assim, pesquisas por métodos de controle não químicos tornam-se indispensáveis para continuidade deste seguimento. Neste contexto, esta tese foi elaborada com os objetivos de avaliar diferentes protocolos experimentais para estabelecimento da infecção de Meriones unguiculatus por H. contortus com e sem imunossupressão (manuscrito 1); de produzir e caracterizar imunoglobulinas Y (IgY) anti H. contortus a partir de larvas de terceiro estágio (L3) (manuscrito 2) e estágios adultos desse helminto (manuscrito 3) e de avaliar a atividade imunoterapêutica da IgY anti-H. contortus em Meriones unguiculatus infectados experimentalmente. Concluímos assim, que é possível estabelecer a infecção de gerbils por H. contortus, sendo a imunossupressão com metilprednisolona a que obteve os melhores resultados comparado a dexametasona e com animais imunocompetentes. Galinhas imunizadas com larvas de terceiro estágio e adultos de H. contortus produziram imunoglobulinas específicas anti-H. contortus e com alta avidez. A imunoterapia interferiu no estabelecimento de H. contortus em gerbils infectados experimentalmente.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessHaemonchus contortusIgYImunoterapiaMeriones unguiculatusHaemonchus contortusIgYImmunotherapyMeriones unguiculatusCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAProdução de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatusProduction of immunoglobulin Y (IgY) anti-Haemonchus contortus e immunotherapy application in Meriones unguiculatusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMonteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Soares, João Fabiohttp://lattes.cnpq.br/0730921999502884Ourique, Aline FerreiraSangioni, Luís AntonioLeal, Marta Lizandra do Regohttp://lattes.cnpq.br/2436787442877449Gressler, Lucas Trevisan500500000007600500500f658c142-7522-4cd5-a133-f2479652bf31cdd9d078-5d60-45d3-ae18-a4e37e28e630f716ade7-351a-43be-bf56-7771ea71560e0a663d9a-ea5e-4bbc-bc43-805607d10dac6bd6f048-e91a-455c-a216-bc6cc0ceb1dae4bbaeee-3ded-43e8-a9d1-c28e6cbf73cdreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGMV_2018_GRESSLER_LUCAS.pdfTES_PPGMV_2018_GRESSLER_LUCAS.pdfTese de Doutoradoapplication/pdf776678http://repositorio.ufsm.br/bitstream/1/14609/1/TES_PPGMV_2018_GRESSLER_LUCAS.pdf47eede93cfea25190acab7334a5f8bd5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
dc.title.alternative.eng.fl_str_mv |
Production of immunoglobulin Y (IgY) anti-Haemonchus contortus e immunotherapy application in Meriones unguiculatus |
title |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
spellingShingle |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus Gressler, Lucas Trevisan Haemonchus contortus IgY Imunoterapia Meriones unguiculatus Haemonchus contortus IgY Immunotherapy Meriones unguiculatus CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
title_full |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
title_fullStr |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
title_full_unstemmed |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
title_sort |
Produção de imunoglobulina Y (IgY) anti-Haemonchus contortus e aplicação imunoterápica em Meriones unguiculatus |
author |
Gressler, Lucas Trevisan |
author_facet |
Gressler, Lucas Trevisan |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Monteiro, Silvia Gonzalez |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3762606653182779 |
dc.contributor.referee1.fl_str_mv |
Soares, João Fabio |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0730921999502884 |
dc.contributor.referee2.fl_str_mv |
Ourique, Aline Ferreira |
dc.contributor.referee3.fl_str_mv |
Sangioni, Luís Antonio |
dc.contributor.referee4.fl_str_mv |
Leal, Marta Lizandra do Rego |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2436787442877449 |
dc.contributor.author.fl_str_mv |
Gressler, Lucas Trevisan |
contributor_str_mv |
Monteiro, Silvia Gonzalez Soares, João Fabio Ourique, Aline Ferreira Sangioni, Luís Antonio Leal, Marta Lizandra do Rego |
dc.subject.por.fl_str_mv |
Haemonchus contortus IgY Imunoterapia Meriones unguiculatus |
topic |
Haemonchus contortus IgY Imunoterapia Meriones unguiculatus Haemonchus contortus IgY Immunotherapy Meriones unguiculatus CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Haemonchus contortus IgY Immunotherapy Meriones unguiculatus |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
In the last decade, among the medium-sized herds (poultry, swine, sheep), the one with the highest growth rate was sheep. For the sheep farming, gastrointestinal helminths can be considered one of the main barriers for breeding today. Among these, we highlight the haemoncosis, caused by the Haemonchus contortus species, a cosmopolitan parasite, highly pathogenic and prevalent. This disease, in addition to causing the death and delayed development of animals, reduces production, resulting in increased breeding costs. Due to the importance of the control of this parasitosis, the massive and indiscriminate use of antihelmintics promoted the selection of multiresistant nematodes and, consequently, the deficient action of the products commercially available. Thus, to research this topic is essential for the prevention and control of infections caused by H. contortus. In this context, this thesis was elaborated with the aim of evaluate different experimental protocols for the establishment of H. contortus infection of Meriones unguiculatus with and without immunosuppression (manuscript 1); to produce and characterize anti-H. contortus immunoglobulins Y (IgY) from third instar larvae (L3) (manuscript 2) and adult stages of this helminth (manuscript 3), and to evaluate the IgY immunotherapeutic activity anti-H. contortus in Meriones unguiculatus experimentally infected. We observed that is possible to establish gerbils’ infection by H. contortus, after immunosuppression with methylprednisolone compared to dexamethasone immunosuppression and immunocompetent animals. Chickens immunized with third-stage larvae and adults of H. contortus produced immunoglobulins anti-H. contortus displaying specificity and high avidity. Lastly, we verified that immunotherapy interfered in the establishment of H. contortus in experimentally infected gerbils. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-10-19T17:41:07Z |
dc.date.available.fl_str_mv |
2018-10-19T17:41:07Z |
dc.date.issued.fl_str_mv |
2018-02-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/14609 |
url |
http://repositorio.ufsm.br/handle/1/14609 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
600 500 500 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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