Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)

Detalhes bibliográficos
Autor(a) principal: Faria, Sophia Iwersen
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/00130000015tg
Texto Completo: http://repositorio.ufsm.br/handle/1/30422
Resumo: Photodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.
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spelling Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)Characterization of genotoxic potential of free base porphyrins and Zn (II) metalloporphyrinsTerapia fotodinâmicaPorfirinas catiônicasPorfirinas aniônicasZn(II)Dano ao DNAMelanomaPhotodynamic therapyCationic porphyrinsAnionic porphyrinsDNA damageCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAPhotodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA terapia fotodinâmica (TDF) é um tratamento com diversos benefícios, como poucos efeitos colaterais, facilidade de aplicação e possível combinação com outros tratamentos. Baseia-se na produção de espécies reativas de oxigênio (ERO) por um fotossensibilizador (FS) após ser irradiado. Devido às suas muitas características desejáveis, as porfirinas são frequentemente usadas como FS em TFD. Alguns de seus atributos podem variar, influenciando sua interação com biomoléculas de interesse. Neste trabalho, quatro compostos foram estudados: TMePyP+, TPPS-, ZnTMe+ e ZnTTP-. Suas interações com o DNA foram avaliadas através de genotoxicidade, produção de EROs e citotoxicidade. Os resultados mostraram que as porfirinas catiônicas foram muito mais eficazes na indução de danos ao DNA, geração de EROs e redução da viabilidade celular do que as aniônicas. A adição de zinco teve pouco efeito sobre esses aspectos, mas fez com que a porfirina se tornasse mais seletiva para células tumorais. Assim, este estudo fornece informações sobre o papel que a carga e a adição de metal desempenham na interação com o DNA, e também demonstra que TMePyP+ e ZnTMe+ são candidatos promissores para uso como PS na TFD.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasSchuch, André Passagliahttp://lattes.cnpq.br/4932611269622766Segatto, Ana Lúcia AnversaVizzoto, Bruno StefanelloFaria, Sophia Iwersen2023-11-01T12:59:17Z2023-11-01T12:59:17Z2023-09-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/30422ark:/26339/00130000015tgporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-11-01T12:59:17Zoai:repositorio.ufsm.br:1/30422Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-11-01T12:59:17Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
Characterization of genotoxic potential of free base porphyrins and Zn (II) metalloporphyrins
title Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
spellingShingle Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
Faria, Sophia Iwersen
Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_full Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_fullStr Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_full_unstemmed Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
title_sort Caracterização do potencial genotóxico de porfirinas de base livre e metaloporfirinas de Zn (II)
author Faria, Sophia Iwersen
author_facet Faria, Sophia Iwersen
author_role author
dc.contributor.none.fl_str_mv Schuch, André Passaglia
http://lattes.cnpq.br/4932611269622766
Segatto, Ana Lúcia Anversa
Vizzoto, Bruno Stefanello
dc.contributor.author.fl_str_mv Faria, Sophia Iwersen
dc.subject.por.fl_str_mv Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Terapia fotodinâmica
Porfirinas catiônicas
Porfirinas aniônicas
Zn(II)
Dano ao DNA
Melanoma
Photodynamic therapy
Cationic porphyrins
Anionic porphyrins
DNA damage
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Photodynamic therapy (PDT) is a treatment with several benefits, such as few side effects, ease of application and possible combination with other treatments. It is based on the production of reactive oxygen species (ROS) by a Photosensitizer (PS) upon irradiation. Due to their many desirable characteristics, porphyrins are often used as PS in PDT. Some of their attributes can vary, influencing its interaction with biomolecules of interest. In this work, four compounds were studied: TMePyP+, TPPS-, ZnTMe+ and ZnTTP-. Their interactions with DNA were evaluated through genotoxicity, ROS production and cytotoxicity. Results showed that cationic porphyrins were way more effective in inducing DNA damage, generating ROS and reducing cell viability than anionic ones. The zinc addition had little effect on those aspects, but caused the porphyrin to be selective towards tumours cells. Thus, this study provides insight on the role that charge and metal addition play in the interaction with DNA, and also that TMePyP+ and ZnTMe+ are promising candidates for use as PS in PDT.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-01T12:59:17Z
2023-11-01T12:59:17Z
2023-09-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/30422
dc.identifier.dark.fl_str_mv ark:/26339/00130000015tg
url http://repositorio.ufsm.br/handle/1/30422
identifier_str_mv ark:/26339/00130000015tg
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Centro de Ciências Naturais e Exatas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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