Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11084 |
Resumo: | Acute Myocardial Infarction (AMI) is one of the major public health problems in the world. Coronary occlusion, which occurs when an atherosclerotic plaque breaks, is the main mechanism that leads to AMI. With the objective of tissue reparation, there is an increase in platelet aggregation and thrombus formation. When ischemia/reperfusion occurs in AMI, Reactive Oxygen Species (ROS) are produced. The imbalance between ROS production and degradation may lead to an increase in oxidative stress. This study aimed to determine the activity of enzymes involved in thromboregulation, such as NTPDase and 5 -nucleotidase, as well as oxidative stress parameters. Evaluation of the oxidant system was carried out by lipid peroxidation and carbonyl protein determination, and the enzymatic and nonenzymatic antioxidant defense measurements were performed in platelets, total blood, plasma, and serum of AMI patients. The results demonstrated that an increase in the activity of NTPDase by ATP (54%) and ADP (45%) hydrolysis occurred in AMI patients when compared to the control group. The same occurred with 5 -nucleotidase activity. The hydrolysis of AMP increased 46% in AMI patients compared to the control group. The increase in ectonucleotidase activities could be related to a compensatory organic response to the pathologic state formed. Regarding oxidant levels, an increase in TBARS and carbonyl protein levels was observed in AMI patients when compared to the control group. The same occurred for the activities of the enzymatic antioxidants, superoxide dismutase (SOD), and catalase (CAT). However, a decrease in nonenzymatic antioxidants, such as vitamin C and vitamin E, was observed in AMI patients when compared to control. These results suggest an increase in oxidative stress in AMI, which was probably a result of the ischemic/reperfusion moment, as well as a decrease of antioxidant defenses. Furthermore, the increased antioxidant defense may act as a compensatory mechanism in consequence of the overproduction of ROS after AMI. No differences in the parameters tested were observed with the drugs utilized in AMI treatment in vitro. In conclusion, AMI results in oxidative damage as well as an increase in the organism s defenses as a compensatory response. |
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Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdioMeasurement of ectonucleotidase activity and oxidative stress indicators in acute myocardial infarction patientsInfarto agudo do miocárdioNTPDase5'-nucleotidasePlaquetasEstresse oxidativoAntioxidantes enzimáticosAntioxidantes não enzimáticosAcute myocardial infarctionPlateletsOxidative stressEnzymatic antioxidantsNonenzymatic antioxidantsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAcute Myocardial Infarction (AMI) is one of the major public health problems in the world. Coronary occlusion, which occurs when an atherosclerotic plaque breaks, is the main mechanism that leads to AMI. With the objective of tissue reparation, there is an increase in platelet aggregation and thrombus formation. When ischemia/reperfusion occurs in AMI, Reactive Oxygen Species (ROS) are produced. The imbalance between ROS production and degradation may lead to an increase in oxidative stress. This study aimed to determine the activity of enzymes involved in thromboregulation, such as NTPDase and 5 -nucleotidase, as well as oxidative stress parameters. Evaluation of the oxidant system was carried out by lipid peroxidation and carbonyl protein determination, and the enzymatic and nonenzymatic antioxidant defense measurements were performed in platelets, total blood, plasma, and serum of AMI patients. The results demonstrated that an increase in the activity of NTPDase by ATP (54%) and ADP (45%) hydrolysis occurred in AMI patients when compared to the control group. The same occurred with 5 -nucleotidase activity. The hydrolysis of AMP increased 46% in AMI patients compared to the control group. The increase in ectonucleotidase activities could be related to a compensatory organic response to the pathologic state formed. Regarding oxidant levels, an increase in TBARS and carbonyl protein levels was observed in AMI patients when compared to the control group. The same occurred for the activities of the enzymatic antioxidants, superoxide dismutase (SOD), and catalase (CAT). However, a decrease in nonenzymatic antioxidants, such as vitamin C and vitamin E, was observed in AMI patients when compared to control. These results suggest an increase in oxidative stress in AMI, which was probably a result of the ischemic/reperfusion moment, as well as a decrease of antioxidant defenses. Furthermore, the increased antioxidant defense may act as a compensatory mechanism in consequence of the overproduction of ROS after AMI. No differences in the parameters tested were observed with the drugs utilized in AMI treatment in vitro. In conclusion, AMI results in oxidative damage as well as an increase in the organism s defenses as a compensatory response.Conselho Nacional de Desenvolvimento Científico e TecnológicoO Infarto Agudo do Miocárdio (IAM) é um dos maiores problemas de saúde pública no mundo. O principal mecanismo que leva ao IAM é a oclusão coronariana que ocorre quando uma placa aterosclerótica sofre fissura. Com o objetivo de reparação tecidual temos um aumento na agregação plaquetária e formação do trombo. Acompanhando o momento de isquemia e reperfusão que ocorre no IAM temos uma produção elevada de Espécies Reativas de Oxigênio (EROs). Um desequilíbrio entre a produção e a degradação de EROs pode levar a geração de estresse oxidativo. Neste trabalho determinaram-se a atividade das enzimas envolvidas na tromboregulação: NTPDase e 5 -nucleotidase, e parâmetros de estresse oxidativo em pacientes que sofreram IAM e em pacientes controles. Foi realizada a avaliação do sistema oxidante através da determinação da peroxidação lipídica e da carbonilação protéica e a medida das defesas antioxidantes enzimáticas e não enzimáticas do organismo, em plaquetas, sangue total, plasma e soro destes pacientes. Os resultados demonstraram um aumento na atividade da NTPDase, revelada através da hidrólise dos nucleotídeos ATP (54%) e ADP (45%), em pacientes com IAM quando comparados com o grupo controle. O mesmo ocorreu com a atividade da enzima 5 -nucleotidase. A hidrólise do AMP aumentou 46% nos pacientes com IAM em relação ao grupo controle. O aumento na atividade das ectonucleotidases pode estar relacionado a uma resposta orgânica compensatória do organismo frente ao estado patológico formado. Em relação aos níveis de oxidantes determinados, observou-se um aumento nos níveis de TBARS e proteína carbonil em soro de pacientes com IAM quando comparados com o grupo controle. Esse aumento também foi observado para as defesas antioxidantes enzimáticas superóxido dismutase (SOD) e catalase (CAT). Entretanto, observou-se um decréscimo das defesas antioxidantes não enzimáticas como a vitamina C e a vitamina E no soro de pacientes com IAM. Estes dados sugerem um aumento do estresse oxidativo como resultado do momento de isquemia/reperfusão e da diminuição das defesas antioxidantes não enzimáticas. Além disso, o aumento das defesas antioxidantes enzimáticas poderiam agir como um mecanismo compensatório como consequência da superprodução de EROS após o IAM. Nenhuma interferência dos medicamentos utilizados no tratamento do IAM foram observadas sobre os parâmetros testados in vitro. Concluí-se então, que o IAM resulta tanto em danos oxidativos como mobilização das defesas do organismo para uma resposta compensatória.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaMorsch, Vera Mariahttp://lattes.cnpq.br/1519648219507868Schetinger, Maria Rosa ChitolinaBurger, Marilise Escobarhttp://lattes.cnpq.br/9128090974948413Rubin, Maribel Antonellohttp://lattes.cnpq.br/7237734243628134Bagatini, Margarete Dulce2017-04-242017-04-242008-03-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBAGATINI, Margarete Dulce. Measurement of ectonucleotidase activity and oxidative stress indicators in acute myocardial infarction patients. 2008. 131 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/11084porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-04-17T13:17:03Zoai:repositorio.ufsm.br:1/11084Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-04-17T13:17:03Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio Measurement of ectonucleotidase activity and oxidative stress indicators in acute myocardial infarction patients |
title |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
spellingShingle |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio Bagatini, Margarete Dulce Infarto agudo do miocárdio NTPDase 5'-nucleotidase Plaquetas Estresse oxidativo Antioxidantes enzimáticos Antioxidantes não enzimáticos Acute myocardial infarction Platelets Oxidative stress Enzymatic antioxidants Nonenzymatic antioxidants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
title_full |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
title_fullStr |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
title_full_unstemmed |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
title_sort |
Medida da atividade de ectonucleotidases e indicadores do estresse oxidativo em pacientes com infarto agudo do miocárdio |
author |
Bagatini, Margarete Dulce |
author_facet |
Bagatini, Margarete Dulce |
author_role |
author |
dc.contributor.none.fl_str_mv |
Morsch, Vera Maria http://lattes.cnpq.br/1519648219507868 Schetinger, Maria Rosa Chitolina Burger, Marilise Escobar http://lattes.cnpq.br/9128090974948413 Rubin, Maribel Antonello http://lattes.cnpq.br/7237734243628134 |
dc.contributor.author.fl_str_mv |
Bagatini, Margarete Dulce |
dc.subject.por.fl_str_mv |
Infarto agudo do miocárdio NTPDase 5'-nucleotidase Plaquetas Estresse oxidativo Antioxidantes enzimáticos Antioxidantes não enzimáticos Acute myocardial infarction Platelets Oxidative stress Enzymatic antioxidants Nonenzymatic antioxidants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Infarto agudo do miocárdio NTPDase 5'-nucleotidase Plaquetas Estresse oxidativo Antioxidantes enzimáticos Antioxidantes não enzimáticos Acute myocardial infarction Platelets Oxidative stress Enzymatic antioxidants Nonenzymatic antioxidants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Acute Myocardial Infarction (AMI) is one of the major public health problems in the world. Coronary occlusion, which occurs when an atherosclerotic plaque breaks, is the main mechanism that leads to AMI. With the objective of tissue reparation, there is an increase in platelet aggregation and thrombus formation. When ischemia/reperfusion occurs in AMI, Reactive Oxygen Species (ROS) are produced. The imbalance between ROS production and degradation may lead to an increase in oxidative stress. This study aimed to determine the activity of enzymes involved in thromboregulation, such as NTPDase and 5 -nucleotidase, as well as oxidative stress parameters. Evaluation of the oxidant system was carried out by lipid peroxidation and carbonyl protein determination, and the enzymatic and nonenzymatic antioxidant defense measurements were performed in platelets, total blood, plasma, and serum of AMI patients. The results demonstrated that an increase in the activity of NTPDase by ATP (54%) and ADP (45%) hydrolysis occurred in AMI patients when compared to the control group. The same occurred with 5 -nucleotidase activity. The hydrolysis of AMP increased 46% in AMI patients compared to the control group. The increase in ectonucleotidase activities could be related to a compensatory organic response to the pathologic state formed. Regarding oxidant levels, an increase in TBARS and carbonyl protein levels was observed in AMI patients when compared to the control group. The same occurred for the activities of the enzymatic antioxidants, superoxide dismutase (SOD), and catalase (CAT). However, a decrease in nonenzymatic antioxidants, such as vitamin C and vitamin E, was observed in AMI patients when compared to control. These results suggest an increase in oxidative stress in AMI, which was probably a result of the ischemic/reperfusion moment, as well as a decrease of antioxidant defenses. Furthermore, the increased antioxidant defense may act as a compensatory mechanism in consequence of the overproduction of ROS after AMI. No differences in the parameters tested were observed with the drugs utilized in AMI treatment in vitro. In conclusion, AMI results in oxidative damage as well as an increase in the organism s defenses as a compensatory response. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-07 2017-04-24 2017-04-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BAGATINI, Margarete Dulce. Measurement of ectonucleotidase activity and oxidative stress indicators in acute myocardial infarction patients. 2008. 131 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/11084 |
identifier_str_mv |
BAGATINI, Margarete Dulce. Measurement of ectonucleotidase activity and oxidative stress indicators in acute myocardial infarction patients. 2008. 131 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
url |
http://repositorio.ufsm.br/handle/1/11084 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922150668828672 |