Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10058 |
Resumo: | Preimplantation genetic diagnosis (PGD) is becoming a current issue in animal reproduction biotechnology due to economical reasons. Predetermining the sex of offspring is one example of PGD. This study aimed to determine the percentage of male and female bovine embryos in vitro produced after oocyte fertilization with Percoll density gradient centrifugation or with self-migration (swim-up) selected semen. In experiment 1, sperm selection was performed by 90%-45% discontinuous Percoll density gradient centrifugation (T1) and swim-up (T2). In experiment 2, along side the discontinuous gradient, a 67.5% continuous density gradient, and centrifugation time of 5 and 10 minutes were used. A total of 4 treatment groups was defined (TI = continuous, 5 minutes, TII = discontinuous, 5 minutes, TIII = continuous, 10 minutes and TIV = discontinuous, 10 minutes). Polymerase chain reaction (PCR) was used to determine the sex of the embryos. T1 (n=185) resulted in 48.65% (n=90) male embryos and 51.35% (n=95) female embryos and T2 (n=142) in 58.45% (n=83) male and 41.55% (n=59) female embryos. In experiment 2, the percentages of male and female embryos obtained in TI (n=93), TII (n=70), TIII (n=82) and TIV (n=82) were 49.46% (n=46) and 50.54% (n=47), 57.14% (n=40) and 42.86% (n=30), 36.59% (n=30) and 63.41% (n=52) and 48.78% (n=40) and 51.22% (n=42), respectively. There was no difference on the percentage of males and females in all treatment groups from experiments 1 and 2 when these were individually compared to the expected percentage of 50% of each sex. There was also no difference in male and female embryo percentage between treatment groups in experiments 1 and 2. |
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2017-06-012017-06-012007-02-27WOLF, Caroline Antoniazzi. Sexing in vitro produced bovine embryos with semen selected by PERCOLL or SWIM-UP. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007.http://repositorio.ufsm.br/handle/1/10058Preimplantation genetic diagnosis (PGD) is becoming a current issue in animal reproduction biotechnology due to economical reasons. Predetermining the sex of offspring is one example of PGD. This study aimed to determine the percentage of male and female bovine embryos in vitro produced after oocyte fertilization with Percoll density gradient centrifugation or with self-migration (swim-up) selected semen. In experiment 1, sperm selection was performed by 90%-45% discontinuous Percoll density gradient centrifugation (T1) and swim-up (T2). In experiment 2, along side the discontinuous gradient, a 67.5% continuous density gradient, and centrifugation time of 5 and 10 minutes were used. A total of 4 treatment groups was defined (TI = continuous, 5 minutes, TII = discontinuous, 5 minutes, TIII = continuous, 10 minutes and TIV = discontinuous, 10 minutes). Polymerase chain reaction (PCR) was used to determine the sex of the embryos. T1 (n=185) resulted in 48.65% (n=90) male embryos and 51.35% (n=95) female embryos and T2 (n=142) in 58.45% (n=83) male and 41.55% (n=59) female embryos. In experiment 2, the percentages of male and female embryos obtained in TI (n=93), TII (n=70), TIII (n=82) and TIV (n=82) were 49.46% (n=46) and 50.54% (n=47), 57.14% (n=40) and 42.86% (n=30), 36.59% (n=30) and 63.41% (n=52) and 48.78% (n=40) and 51.22% (n=42), respectively. There was no difference on the percentage of males and females in all treatment groups from experiments 1 and 2 when these were individually compared to the expected percentage of 50% of each sex. There was also no difference in male and female embryo percentage between treatment groups in experiments 1 and 2.O diagnóstico genético pré-implantação (DGP) vem se destacando na área da biotecnologia da reprodução animal por motivos econômicos. Um exemplo de DGP é a predeterminação do sexo da prole. Neste estudo foi verificada a percentagem de embriões bovinos machos e fêmeas produzidos in vitro após a fertilização de oócitos com sêmen selecionado por centrifugação em gradiente de densidade de Percoll ou por migração ascendente (swim-up). No experimento 1 a seleção espermática foi realizada usando o gradiente descontínuo de Percoll de 90% e 45% (T1) e o swimup (T2). No experimento 2 foi utilizado, além do gradiente descontínuo, um gradiente contínuo de densidade de Percoll de 67,5%, e tempos de centrifugação de 5 e 10 minutos, totalizando 4 tratamentos (TI = contínuo 5 minutos, TII = descontínuo 5 minutos, TIII = contínuo 10 minutos e TIV = descontínuo 10 minutos). A sexagem dos embriões foi realizada através da técnica da reação em cadeia da polimerase (PCR). No T1 (n=185) foram obtidos 48,65% (n=90) de embriões masculinos e 51,35% (n=95) de femininos e no T2 (n=142) 58,45% (n=83) foram machos e 41,55% (n=59) fêmeas. No experimento 2, a percentagem de embriões masculinos e femininos no TI (n=93), TII (n=70), TIII (n=82) e TIV (n=82) foi de 49,46% (n=46) e 50,54% (n=47), 57,14% (n=40) e 42,86% (n=30), 36,59% (n=30) e 63,41% (n=52), e 48,78% (n=40) e 51,22% (n=42), respectivamente. Não houve alteração na percentagem de machos e fêmeas nos tratamentos dos experimentos 1 e 2 quando estes tratamentos foram comparados individualmente com a percentagem teoricamente esperada de 50% de cada sexo. Também não houve alteração na percentagem de machos e fêmeas na comparação entre os dois tratamentos do experimento 1 e entre os quatro tratamentos do experimento 2.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaSeleção espermáticaGradiente de densidadeMigração ascendenteFIVCromossomos X e YDNASperm selectionDensity gradientSelf-migrationIVFX and Y chromosomesDNACNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIASexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-upSexing in vitro produced bovine embryos with semen selected by Percoll or Swim-upinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBrass, Karin Ericahttp://lattes.cnpq.br/7502192989660220Neves, Adriana Pireshttp://lattes.cnpq.br/9029302789722486Schossler, Deila Rosely Carneirohttp://lattes.cnpq.br/2268564401345390http://lattes.cnpq.br/8699189483153862Wolf, Caroline Antoniazzi500500000007400300300500300ca3e85b8-83e6-4d25-afb2-2e71a36cebce32d0c561-267e-4aa8-9c81-76ace9d477c62e70ebbc-39cd-46fc-a723-6b858c3b06dd4c6f071e-c078-4a7f-8549-c33ba67bd0cdinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALWOLF, CAROLINE ANTONIAZZI.pdfapplication/pdf185843http://repositorio.ufsm.br/bitstream/1/10058/1/WOLF%2c%20CAROLINE%20ANTONIAZZI.pdf252c140fc72b9f235717493d0efdbc7aMD51TEXTWOLF, CAROLINE ANTONIAZZI.pdf.txtWOLF, CAROLINE ANTONIAZZI.pdf.txtExtracted texttext/plain67261http://repositorio.ufsm.br/bitstream/1/10058/2/WOLF%2c%20CAROLINE%20ANTONIAZZI.pdf.txtdc50f7106d8523a570675877d570e748MD52THUMBNAILWOLF, CAROLINE ANTONIAZZI.pdf.jpgWOLF, CAROLINE ANTONIAZZI.pdf.jpgIM Thumbnailimage/jpeg4784http://repositorio.ufsm.br/bitstream/1/10058/3/WOLF%2c%20CAROLINE%20ANTONIAZZI.pdf.jpg507f9241c6c2d06587a8248b6af3f4cdMD531/100582023-03-03 10:47:50.149oai:repositorio.ufsm.br:1/10058Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-03-03T13:47:50Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
dc.title.alternative.eng.fl_str_mv |
Sexing in vitro produced bovine embryos with semen selected by Percoll or Swim-up |
title |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
spellingShingle |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up Wolf, Caroline Antoniazzi Seleção espermática Gradiente de densidade Migração ascendente FIV Cromossomos X e Y DNA Sperm selection Density gradient Self-migration IVF X and Y chromosomes DNA CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
title_full |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
title_fullStr |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
title_full_unstemmed |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
title_sort |
Sexagem de embriões bovinos produzidos in vitro com sêmen selecionado por Percoll ou Swim-up |
author |
Wolf, Caroline Antoniazzi |
author_facet |
Wolf, Caroline Antoniazzi |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Brass, Karin Erica |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7502192989660220 |
dc.contributor.referee1.fl_str_mv |
Neves, Adriana Pires |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9029302789722486 |
dc.contributor.referee2.fl_str_mv |
Schossler, Deila Rosely Carneiro |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2268564401345390 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8699189483153862 |
dc.contributor.author.fl_str_mv |
Wolf, Caroline Antoniazzi |
contributor_str_mv |
Brass, Karin Erica Neves, Adriana Pires Schossler, Deila Rosely Carneiro |
dc.subject.por.fl_str_mv |
Seleção espermática Gradiente de densidade Migração ascendente FIV Cromossomos X e Y DNA |
topic |
Seleção espermática Gradiente de densidade Migração ascendente FIV Cromossomos X e Y DNA Sperm selection Density gradient Self-migration IVF X and Y chromosomes DNA CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Sperm selection Density gradient Self-migration IVF X and Y chromosomes DNA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Preimplantation genetic diagnosis (PGD) is becoming a current issue in animal reproduction biotechnology due to economical reasons. Predetermining the sex of offspring is one example of PGD. This study aimed to determine the percentage of male and female bovine embryos in vitro produced after oocyte fertilization with Percoll density gradient centrifugation or with self-migration (swim-up) selected semen. In experiment 1, sperm selection was performed by 90%-45% discontinuous Percoll density gradient centrifugation (T1) and swim-up (T2). In experiment 2, along side the discontinuous gradient, a 67.5% continuous density gradient, and centrifugation time of 5 and 10 minutes were used. A total of 4 treatment groups was defined (TI = continuous, 5 minutes, TII = discontinuous, 5 minutes, TIII = continuous, 10 minutes and TIV = discontinuous, 10 minutes). Polymerase chain reaction (PCR) was used to determine the sex of the embryos. T1 (n=185) resulted in 48.65% (n=90) male embryos and 51.35% (n=95) female embryos and T2 (n=142) in 58.45% (n=83) male and 41.55% (n=59) female embryos. In experiment 2, the percentages of male and female embryos obtained in TI (n=93), TII (n=70), TIII (n=82) and TIV (n=82) were 49.46% (n=46) and 50.54% (n=47), 57.14% (n=40) and 42.86% (n=30), 36.59% (n=30) and 63.41% (n=52) and 48.78% (n=40) and 51.22% (n=42), respectively. There was no difference on the percentage of males and females in all treatment groups from experiments 1 and 2 when these were individually compared to the expected percentage of 50% of each sex. There was also no difference in male and female embryo percentage between treatment groups in experiments 1 and 2. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-02-27 |
dc.date.accessioned.fl_str_mv |
2017-06-01 |
dc.date.available.fl_str_mv |
2017-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
WOLF, Caroline Antoniazzi. Sexing in vitro produced bovine embryos with semen selected by PERCOLL or SWIM-UP. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10058 |
identifier_str_mv |
WOLF, Caroline Antoniazzi. Sexing in vitro produced bovine embryos with semen selected by PERCOLL or SWIM-UP. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007. |
url |
http://repositorio.ufsm.br/handle/1/10058 |
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UFSM |
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BR |
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Medicina Veterinária |
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Universidade Federal de Santa Maria |
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