Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/30991 |
Resumo: | Soy cultivated in lowland areas is constantly expanding, being rotated in the rice soils of Rio Grande do Sul, mainly due to the economic appreciation and benefits generated by rice. However, in lowlands soybeans can be affected by various stresses, so it is essential to adopt integrated physical, chemical and biological tools to solve agricultural problems. The use of bioinputs can help with biological nitrogen fixation, promoting plant metabolism, reducing incidences of diseases, among others. However, the way in which it will be made available may influence its efficiency. With this, the objective was to evaluate the effects of inoculation and coinoculation of Bradyrhizobium japonicum with multifunctional microorganisms in soybean crops, and the best way to apply them via seed treatment and sowing furrow in lowland environments. In the first chapter, an experiment was carried out in a greenhouse, in the Várzea experimental teaching area at UFSM, using a completely randomized experimental design, with four replications and ten treatments: 1) Control; 2) Bradyrhizobium japonicum (Bj); 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. pumilus; 8) Bj + B. amyloliquefaciens; 9) Bj + B. megaterium; 10) Bj + Bio bokashi. At 50 days after installation, biometric, physiological and chemical evaluations were carried out on the plants. The results identified that the inoculation and co-inoculation of microorganisms does not affect the aerial part and the NPK levels of the plant leaf tissue, but positively influences the root system. Inoculation of B. japonicum presents an average increase of 60.8% in root dry mass (MSR), 60.7% in the number of nodules (NN) and 66.6% in root volume (VR), also presenting greater net carbon assimilation rate and water use efficiency. Coinoculation with other bacteria stimulates a greater supply of dry root mass in plants, with emphasis on A. brasilense, which increased MSR, NN and VR when compared to simple inoculation, making it a good choice for coinoculation. In the second chapter, two field experiments were carried out, one in the municipality of Santa Maria-RS and the other in Candelária-RS, using a randomized block experimental design in a split plot, bifactorial 2 x 8, with four replications. Two forms of application were distributed in the main plots: (seed and sowing furrow) and in the subplots eight associations between microorganisms: 2) Bj; 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. amyloliquefaciens; 8) Bj + B. megaterium. Measurements of biometric, physiological and chemical variables in plants and grain productivity were carried out. With the main results obtained, it is observed that inoculation with B. japonicum is efficient and co-inoculation with bacteria, especially A. brasilense, has positive effects on the root system of plants, on the coverage rate of the vegetative canopy and on productivity. of soybeans, especially when applied in the sowing furrow. |
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Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixasinoculation and coinoculation of microorganisms via seed and sowing furrow in soybean in lowlandsBactérias multifuncionaisBioinsumosFixação biológica de nitrogênioFormas de aplicaçãoGlycine max L.Multifunctional bacteriaBioinputsBiological nitrogen fixationForms of applicationCNPQ::CIENCIAS AGRARIAS::AGRONOMIASoy cultivated in lowland areas is constantly expanding, being rotated in the rice soils of Rio Grande do Sul, mainly due to the economic appreciation and benefits generated by rice. However, in lowlands soybeans can be affected by various stresses, so it is essential to adopt integrated physical, chemical and biological tools to solve agricultural problems. The use of bioinputs can help with biological nitrogen fixation, promoting plant metabolism, reducing incidences of diseases, among others. However, the way in which it will be made available may influence its efficiency. With this, the objective was to evaluate the effects of inoculation and coinoculation of Bradyrhizobium japonicum with multifunctional microorganisms in soybean crops, and the best way to apply them via seed treatment and sowing furrow in lowland environments. In the first chapter, an experiment was carried out in a greenhouse, in the Várzea experimental teaching area at UFSM, using a completely randomized experimental design, with four replications and ten treatments: 1) Control; 2) Bradyrhizobium japonicum (Bj); 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. pumilus; 8) Bj + B. amyloliquefaciens; 9) Bj + B. megaterium; 10) Bj + Bio bokashi. At 50 days after installation, biometric, physiological and chemical evaluations were carried out on the plants. The results identified that the inoculation and co-inoculation of microorganisms does not affect the aerial part and the NPK levels of the plant leaf tissue, but positively influences the root system. Inoculation of B. japonicum presents an average increase of 60.8% in root dry mass (MSR), 60.7% in the number of nodules (NN) and 66.6% in root volume (VR), also presenting greater net carbon assimilation rate and water use efficiency. Coinoculation with other bacteria stimulates a greater supply of dry root mass in plants, with emphasis on A. brasilense, which increased MSR, NN and VR when compared to simple inoculation, making it a good choice for coinoculation. In the second chapter, two field experiments were carried out, one in the municipality of Santa Maria-RS and the other in Candelária-RS, using a randomized block experimental design in a split plot, bifactorial 2 x 8, with four replications. Two forms of application were distributed in the main plots: (seed and sowing furrow) and in the subplots eight associations between microorganisms: 2) Bj; 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. amyloliquefaciens; 8) Bj + B. megaterium. Measurements of biometric, physiological and chemical variables in plants and grain productivity were carried out. With the main results obtained, it is observed that inoculation with B. japonicum is efficient and co-inoculation with bacteria, especially A. brasilense, has positive effects on the root system of plants, on the coverage rate of the vegetative canopy and on productivity. of soybeans, especially when applied in the sowing furrow.A soja cultivada em áreas de terras baixas está em constante expansão, sendo rotacionada nos solos arrozeiros do Rio Grande do Sul, principalmente devido à valorização econômica e os benefícios gerados ao arroz. No entanto, em terras baixas a soja pode ser afetada por diversos estresses, logo é imprescindível a adoção de ferramentas integradas de ordem física, química e biológica para a solução de problemas agrícolas. A utilização de bioinsumos pode ajudar na fixação biológica de nitrogênio, promoção do metabolismo da planta, diminuir incidências de doenças, dentre outras. Porém, a forma como irá ser disponibilizado poderá influenciar na eficiência do mesmo. Com isso, objetivou-se avaliar os efeitos da inoculação e coinoculação de Bradyrhizobium japonicum com microrganismos multifuncionais na cultura da soja, e a melhor forma de aplicação destes via tratamento de sementes e sulco de semeadura em ambiente de terras baixas. No primeiro capítulo, foi realizado um experimento em casa de vegetação, na área didático experimental de Várzea da UFSM, utilizando o delineamento experimental inteiramente casualizado, com quatro repetições e dez tratamentos: 1) Testemunha (sem inoculação); 2) Bradyrhizobium japonicum (Bj); 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. pumilus; 8) Bj + B. amyloliquefaciens; 9) Bj + B. megaterium; 10) Bj + Bio bokashi. Aos 50 dias após a instalação, foram realizadas avaliações biométricas, fisiológicas e químicas nas plantas. Os resultados identificaram que a inoculação e coinoculação de microrganismos não interfere na parte aérea e nos teores de NPK do tecido foliar das plantas, mas, influência positivamente o sistema radicular. A inoculação de B. japonicum apresenta incrementos médio de 60,8% na massa seca das raízes (MSR), 60,7% no número de nódulos (NN) e 66,6% no volume radicular (VR), apresentando também, maior taxa líquida de assimilação de carbono e eficiência do uso da água. As coinoculação com as demais bactérias estimula um maior aporte de massa seca de raiz nas plantas, com destaque para o A. brasilense a qual incrementou MSR, NN e VR quando comparada a inoculação simples, sendo está uma boa escolha para coinoculação. No segundo capítulo, foram realizados dois experimentos a campo, um no município de Santa Maria-RS e outro em Candelária-RS, utilizando o delineamento experimental de blocos ao acaso em parcela subdividida, bifatorial 2 x 8, com quatro repetições. Nas parcelas principais foram distribuídas duas formas de aplicação: (semente e sulco de semeadura) e nas subparcelas oito associações entre microrganismos: 1) Testemunha; 2) Bj; 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. amyloliquefaciens; 8) Bj + B. megaterium. Realizou-se mensurações de variáveis biométricas, fisiológicas, químicas nas plantas e de produtividade de grãos. Com os principais resultados obtidos, observa-se que a inoculação com B. japonicum é eficiente e a coinoculação com bactérias em especial com o A. brasilense, apresenta efeitos positivos no sistema radicular das plantas, na taxa de cobertura do dossel vegetativo e na produtividade de grãos de soja, principalmente quando aplicada no sulco de semeadura.Universidade Federal de Santa MariaBrasilAgronomiaUFSMPrograma de Pós-Graduação em AgronomiaCentro de Ciências RuraisMarchesan, Eniohttp://lattes.cnpq.br/2740617028203037Martin, Thomas NewtonOsorio Filho, Benjamin DiasFipke , Glauber MonçonNunes, Rafaela Leopoldina Silva2023-12-28T14:37:34Z2023-12-28T14:37:34Z2023-08-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/30991porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-12-28T14:37:34Zoai:repositorio.ufsm.br:1/30991Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-12-28T14:37:34Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas inoculation and coinoculation of microorganisms via seed and sowing furrow in soybean in lowlands |
title |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
spellingShingle |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas Nunes, Rafaela Leopoldina Silva Bactérias multifuncionais Bioinsumos Fixação biológica de nitrogênio Formas de aplicação Glycine max L. Multifunctional bacteria Bioinputs Biological nitrogen fixation Forms of application CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
title_full |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
title_fullStr |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
title_full_unstemmed |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
title_sort |
Inoculação e coinoculação de microrganismos via semente e sulco de semeadura na soja em terras baixas |
author |
Nunes, Rafaela Leopoldina Silva |
author_facet |
Nunes, Rafaela Leopoldina Silva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Marchesan, Enio http://lattes.cnpq.br/2740617028203037 Martin, Thomas Newton Osorio Filho, Benjamin Dias Fipke , Glauber Monçon |
dc.contributor.author.fl_str_mv |
Nunes, Rafaela Leopoldina Silva |
dc.subject.por.fl_str_mv |
Bactérias multifuncionais Bioinsumos Fixação biológica de nitrogênio Formas de aplicação Glycine max L. Multifunctional bacteria Bioinputs Biological nitrogen fixation Forms of application CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
topic |
Bactérias multifuncionais Bioinsumos Fixação biológica de nitrogênio Formas de aplicação Glycine max L. Multifunctional bacteria Bioinputs Biological nitrogen fixation Forms of application CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Soy cultivated in lowland areas is constantly expanding, being rotated in the rice soils of Rio Grande do Sul, mainly due to the economic appreciation and benefits generated by rice. However, in lowlands soybeans can be affected by various stresses, so it is essential to adopt integrated physical, chemical and biological tools to solve agricultural problems. The use of bioinputs can help with biological nitrogen fixation, promoting plant metabolism, reducing incidences of diseases, among others. However, the way in which it will be made available may influence its efficiency. With this, the objective was to evaluate the effects of inoculation and coinoculation of Bradyrhizobium japonicum with multifunctional microorganisms in soybean crops, and the best way to apply them via seed treatment and sowing furrow in lowland environments. In the first chapter, an experiment was carried out in a greenhouse, in the Várzea experimental teaching area at UFSM, using a completely randomized experimental design, with four replications and ten treatments: 1) Control; 2) Bradyrhizobium japonicum (Bj); 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. pumilus; 8) Bj + B. amyloliquefaciens; 9) Bj + B. megaterium; 10) Bj + Bio bokashi. At 50 days after installation, biometric, physiological and chemical evaluations were carried out on the plants. The results identified that the inoculation and co-inoculation of microorganisms does not affect the aerial part and the NPK levels of the plant leaf tissue, but positively influences the root system. Inoculation of B. japonicum presents an average increase of 60.8% in root dry mass (MSR), 60.7% in the number of nodules (NN) and 66.6% in root volume (VR), also presenting greater net carbon assimilation rate and water use efficiency. Coinoculation with other bacteria stimulates a greater supply of dry root mass in plants, with emphasis on A. brasilense, which increased MSR, NN and VR when compared to simple inoculation, making it a good choice for coinoculation. In the second chapter, two field experiments were carried out, one in the municipality of Santa Maria-RS and the other in Candelária-RS, using a randomized block experimental design in a split plot, bifactorial 2 x 8, with four replications. Two forms of application were distributed in the main plots: (seed and sowing furrow) and in the subplots eight associations between microorganisms: 2) Bj; 3) Bj + A. brasilense; 4) Bj + T. asperelloides; 5) Bj + P. fluorescens; 6) Bj + B. subtilis; 7) Bj + B. amyloliquefaciens; 8) Bj + B. megaterium. Measurements of biometric, physiological and chemical variables in plants and grain productivity were carried out. With the main results obtained, it is observed that inoculation with B. japonicum is efficient and co-inoculation with bacteria, especially A. brasilense, has positive effects on the root system of plants, on the coverage rate of the vegetative canopy and on productivity. of soybeans, especially when applied in the sowing furrow. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-28T14:37:34Z 2023-12-28T14:37:34Z 2023-08-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/30991 |
url |
http://repositorio.ufsm.br/handle/1/30991 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Agronomia UFSM Programa de Pós-Graduação em Agronomia Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Agronomia UFSM Programa de Pós-Graduação em Agronomia Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922110364712960 |