Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos

Detalhes bibliográficos
Autor(a) principal: Anwar, Javed
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000k08f
Texto Completo: http://repositorio.ufsm.br/handle/1/4459
Resumo: Phenolic compouds and their derivatives constitute a leading family of natural compounds. Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) are the important members of phenolic compound, sharing several biological applications; antioxidant, neuroprotective, anti-inflammatory, antiproliferative, antibacterial, antiviral, antiatherosclerotic and anticancer properties. In spite of these, literature reportes some of its pro-oxidants activity depending on cellular environment. These pathophysiolocal properties increased the interest to evaluate the effect of CA and CAPE on the enzyme evolved in the purines salvage and the acetylcholine hydrolyzing enzyme the acetylcholineterase (AChE); in both PNS and CNS, since the essential constituent of our dietary items. Previously, our research group has reported that phenolic compound altered the activities of these enzymes. The AChE rapidly hydrolyzes the acetylcholine in neuronal and non neuronal tissues, mediating several neurodegenerative diseases. Beside the ACh, ATP (as co-neurotramittors) and adenosine are important signaling molecules, communicating cells in both PNS and CNS. In the extracellular signalling pathways; the adenine nucleotides, their derivative and the coupling of these molecules with specific receptor have a crucial role in nervous, vascular and immune systems. Once released, these molecules are hydrolyzed by a cascade of enzymes including ectonucleoside triphosphate diphosphohydrolase (NTPDase; E.C. 3.6.1.5, CD39), 5 -nucleotidase (E.C 3.1.3.5, CD73), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), modulating crucially the signaling pathways in the normal functioning of nervous, vascular and immune systems. Next, adenosine deaminase (ADA) and xanthin oxidase (XO) degrade the adenosine and xanthine respectively which further control the functioning mechanisms in cellular events. Found in the neuronal and non neuronal (in both PNS and CNS) the AChE, NTPDase, 5'-nucleotidase, E-NPP, and ADA regulate several events including neurotransmission, inflammation, and thrombogenic process. We hypothesized to evaluate first the in vitro effects of CA on AChE activity in peripheral and central cholinergic system of rats. The results showed that CA significantly modulated the cholinergic system in vitro. By modulating the cholinergic system in vitro, apparently CA (e.g. phenolic structure) has proper role in neurotransmission. Therefore we hypothesizes to evaluate the in vivo effects of CA on AChE, NTPDase, E-NPP, 5'-nucleotidase, platelets aggregation and (ADA) in different tissues/cell from rats. The animals were treated during 30 days and killed after behavioral test. The results showed that caffeic acid increased significantly the AChE activity in hippocampus, hyphothalmus, pon and lymphocytes while that in cortex, cerebellum and striatum the AChE was inhibited. CA improves step-down latencies in the inhibitory avoidance. Investigating the in vivo effects of CA in purinergic system, caffecid acid increased the ATP and AMP hydrolysis in synaptosomes. However, in the synaptosomes no alterations were observed in the ADA activity in the groups evaluated in this study. CA increased the ATP and AMP hydrolysis, while the ADP hydrolysis was decreased in platelets. In the present findings caffeic acid decreased the platelets aggregation induced by ADP agonist. Treatment with CA also increased the NTPDase and ADA activities in lymphocytes of rats. Considering the dual function of caffeic acid in vitro and in vivo, the present study was extended to CAPE followed by acute treatment model (ip) in order to elucidate the effect of another phenolic structure on the same parameters. In this line the animals were treated (ip) with CAPE and killed after 40 minutes. In platelets, the results showed that the effect of CAPE increased the NTPDase, E-NPP, 5 -nucleotidase activities, while ADA activities did not change significantly. In synaptosomes CAPE significantly inhibited the NTPDase, and 5 -nucleotidase activity. CAPE induced no significant changes in ADA in synaptosomes but reduced XO in whole brain. Finaly we investigated the activity of AChE in cortex, cerebellum, striatum, hippocampus, hyphotalamus, pon, lymphocytes and muscules of rats treated with CAPE. The results showed that CAPE significantly decreased the AChE activity in cortex cerebellum and striatum. CAPE significantly increased the AChE activity in hippocampus hypothalamus, pons, muscle and lymphocytes. In cholinergic system our results clearly demonstrating that both compound with dual functions.These findings demonstrated that the AChE activities and the cascade of ecto-enzymes was alter in different tissues after treatment with CA and CAPE in rats, suggesting that these compound should be considered a potentially therapeutic agent in immune, vascular and neurological disorders related with the cholinergic purinergic system.
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spelling Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratosTHE EFFECTS OF CAFFEIC ACID AND CAFFEIC ACID PHENETHYL ESTER ON THE ACTIVITIES OF ACETYLCHOLINESTERASE AND ECTO-NUCLEOTIDASES IN RATSAcetilcolinesteraseAcetilcolinaNucleotídeo de adeninaEctonucleotidasesPurinoreceptoresÁcido cafeicoÉster fenetil do ácido cafeicoActylcholinesteraseActylcholineAdenin nucleotidesEcto-nucleotidasesPurinoreceptorsCaffeic acidCaffeic acid phenethyl esterCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAPhenolic compouds and their derivatives constitute a leading family of natural compounds. Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) are the important members of phenolic compound, sharing several biological applications; antioxidant, neuroprotective, anti-inflammatory, antiproliferative, antibacterial, antiviral, antiatherosclerotic and anticancer properties. In spite of these, literature reportes some of its pro-oxidants activity depending on cellular environment. These pathophysiolocal properties increased the interest to evaluate the effect of CA and CAPE on the enzyme evolved in the purines salvage and the acetylcholine hydrolyzing enzyme the acetylcholineterase (AChE); in both PNS and CNS, since the essential constituent of our dietary items. Previously, our research group has reported that phenolic compound altered the activities of these enzymes. The AChE rapidly hydrolyzes the acetylcholine in neuronal and non neuronal tissues, mediating several neurodegenerative diseases. Beside the ACh, ATP (as co-neurotramittors) and adenosine are important signaling molecules, communicating cells in both PNS and CNS. In the extracellular signalling pathways; the adenine nucleotides, their derivative and the coupling of these molecules with specific receptor have a crucial role in nervous, vascular and immune systems. Once released, these molecules are hydrolyzed by a cascade of enzymes including ectonucleoside triphosphate diphosphohydrolase (NTPDase; E.C. 3.6.1.5, CD39), 5 -nucleotidase (E.C 3.1.3.5, CD73), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), modulating crucially the signaling pathways in the normal functioning of nervous, vascular and immune systems. Next, adenosine deaminase (ADA) and xanthin oxidase (XO) degrade the adenosine and xanthine respectively which further control the functioning mechanisms in cellular events. Found in the neuronal and non neuronal (in both PNS and CNS) the AChE, NTPDase, 5'-nucleotidase, E-NPP, and ADA regulate several events including neurotransmission, inflammation, and thrombogenic process. We hypothesized to evaluate first the in vitro effects of CA on AChE activity in peripheral and central cholinergic system of rats. The results showed that CA significantly modulated the cholinergic system in vitro. By modulating the cholinergic system in vitro, apparently CA (e.g. phenolic structure) has proper role in neurotransmission. Therefore we hypothesizes to evaluate the in vivo effects of CA on AChE, NTPDase, E-NPP, 5'-nucleotidase, platelets aggregation and (ADA) in different tissues/cell from rats. The animals were treated during 30 days and killed after behavioral test. The results showed that caffeic acid increased significantly the AChE activity in hippocampus, hyphothalmus, pon and lymphocytes while that in cortex, cerebellum and striatum the AChE was inhibited. CA improves step-down latencies in the inhibitory avoidance. Investigating the in vivo effects of CA in purinergic system, caffecid acid increased the ATP and AMP hydrolysis in synaptosomes. However, in the synaptosomes no alterations were observed in the ADA activity in the groups evaluated in this study. CA increased the ATP and AMP hydrolysis, while the ADP hydrolysis was decreased in platelets. In the present findings caffeic acid decreased the platelets aggregation induced by ADP agonist. Treatment with CA also increased the NTPDase and ADA activities in lymphocytes of rats. Considering the dual function of caffeic acid in vitro and in vivo, the present study was extended to CAPE followed by acute treatment model (ip) in order to elucidate the effect of another phenolic structure on the same parameters. In this line the animals were treated (ip) with CAPE and killed after 40 minutes. In platelets, the results showed that the effect of CAPE increased the NTPDase, E-NPP, 5 -nucleotidase activities, while ADA activities did not change significantly. In synaptosomes CAPE significantly inhibited the NTPDase, and 5 -nucleotidase activity. CAPE induced no significant changes in ADA in synaptosomes but reduced XO in whole brain. Finaly we investigated the activity of AChE in cortex, cerebellum, striatum, hippocampus, hyphotalamus, pon, lymphocytes and muscules of rats treated with CAPE. The results showed that CAPE significantly decreased the AChE activity in cortex cerebellum and striatum. CAPE significantly increased the AChE activity in hippocampus hypothalamus, pons, muscle and lymphocytes. In cholinergic system our results clearly demonstrating that both compound with dual functions.These findings demonstrated that the AChE activities and the cascade of ecto-enzymes was alter in different tissues after treatment with CA and CAPE in rats, suggesting that these compound should be considered a potentially therapeutic agent in immune, vascular and neurological disorders related with the cholinergic purinergic system.Conselho Nacional de Desenvolvimento Científico e TecnológicoOs compostos fenólicos e seus derivados constituem uma importante família de compostos naturais. O ácido cafeico (AC) e o éster fenetil do ácido cafeico (CAPE) são membros importantes dessa família e compartilham algumas aplicações biológicas, tais como: antioxidante, neuroprotetor, antiinflamatório, antiproliferativo, antibacteriano, antiviral, antiaterosclerótico e anticancerígeno. Entretanto, a literatura relata algumas atividades pró-oxidantes, dependendo do ambiente celular. Devido a estas propriedades patofisiológicas, aumentou o interesse com o objetivo de avaliar o efeito de CA e CAPE sob as atividades das enzimas purinérgicas e da acetilcolinesterase (AChE), tanto no Sistema Nervoso Periférico (SNP) como no Sistema Nervoso Central (SNC). Previamente, nosso grupo de pesquisa relatou que o composto fenólico tem a capacidade de alterar as atividades dessas enzimas. A AChE rapidamente hidrolisa a acetilcolina (ACh) em tecidos neuronais e não neuronais, mediando algumas doenças neurodegenerativas. Ao lado da ACh, o ATP (como coneurotransmissor) e adenosina são importantes moléculas sinalizadoras, comunicando as células em ambos os SNP e do SNC. Nas vias de sinalização extracelulares, os nucleotídeos de adenina e seus derivados podem ser acoplados a receptores específicos e desse modo ter um papel crucial no sistema nervoso, sistema vascular e imune. Uma vez liberadas, estas moléculas são hidrolisadas por uma cascata de enzimas incluindo a ectonucleosídeo trifosfato difosfoidrolase (NTPDase; EC 3.6.1.5, CD39), 5'- nucleotidase (EC 3.1.3.5, CD73), ectonucleotideo pirofosfatase/fosfodiesterase (E-NPP), modulando definitivamente as vias de sinalização do funcionamento normal do sistema nervoso, sistema vascular e imune. Além disso, a adenosina deaminase (ADA) e a xantina oxidase (XO) degradam a adenosina e a xantina, respectivamente, as quais controlam o funcionamento de mecanismos em eventos celulares. As enzimas encontradas em tecidos neuronais e não neuronais como a AChE, a NTPDase, a 5'-nucleotidase, a E-NPP e a ADA regulam eventos celulares incluindo a neurotransmissão, inflamação e processos trombogênicos. Com essas informações, nós introduzimos a hipótese de avaliar primeiramente os efeitos in vitro de CA na atividade da AChE periférica e no sistema central colinérgico de ratos. Os resultados demonstraram que o CA modula significativamente o sistema colinérgico no estudo in vitro. Essa modulação demonstra aparentemente que o CA (estrutura fenólica) possui propriedades de ação que altera a neurotransmissão. Portanto, a hipótese de se avaliar os efeitos in vivo de CA na atividade da AChE, NTPDase, E-NPP, 5'-nucleotidase, ADA e da agregação de plaquetas em diferentes tecidos de ratos tornou-se evidente. Para esse estudo, os animais foram tratados durante 30 dias e sacrificados após o teste comportamental. Os resultados do experimento demonstraram que o CA aumentou significativamente a atividade da AChE em hipocampo, hipotálamo, ponte e nos linfócitos, enquanto que no córtex cerebral, cerebelo e estriado a AChE foi inibida. No teste comportamental o CA teve evolução de melhora na latência de passos da esquiva inibitória. A investigação dos efeitos in vivo do CA no sistema purinérgico demonstrou aumento na hidrólise de ATP e AMP em sinaptossomas. Entretanto, não foram observadas alterações significativas na atividade da ADA em sinaptossomas dos grupos avaliados neste estudo. Em plaquetas, o CA aumentou significativamente a hidrólise de ATP e AMP, enquanto que a hidrólise de ADP foi diminuída nesse tecido. No presente estudo o CA reduziu significativamente a agregação de plaquetas induzida pelo agonista ADP. Além disso, o tratamento com CA aumentou significativamente as atividades da NTPDase e da ADA em linfócitos de ratos. Considerando a dupla função de CA, in vitro e in vivo, o presente estudo foi estendido para CAPE seguindo o modelo de tratamento agudo pela via intraperitoneal (ip) com o objetivo de elucidar o efeito de uma segunda estrutura fenólica sobre os mesmos parâmetros. Nesta linha de pesquisa, os animais foram tratados ip com CAPE e eutanasiados após 40 minutos. Em plaquetas, os resultados demonstraram que o CAPE aumentou significativamente a atividade da NTPDase, E-NPP e 5'-nucleotidase, enquanto que a atividade da ADA não foi alterada significativamente. Em sinaptossomas, o CAPE inibiu significativamente a atividade da NTPDase e da 5'-nucleotidase. O CAPE não induziu alterações significativas na atividade da ADA em sinaptossomas, mas reduziu significativamente a atividade da XO em todo o cérebro. Finalmente, nós investigamos a atividade da AChE no córtex cerebral, cerebelo, estriado, hipocampo, hipotálamo, ponte, linfócitos e músculos de ratos tratados com CAPE. Os resultados demonstraram que CAPE diminuiu significativamente a atividade da AChE em córtex cerebral, cerebelo e estriado. O CAPE aumentou significativamente a atividade da AChE em hipotálamo, hipocampo, ponte, músculo e linfócitos. No sistema colinérgico, nossos resultados demonstram claramente que ambos os compostos possuem dupla função. Estes resultados demonstram que as atividades da AChE e da cascata das ecto-enzimas foram alteradas em diferentes tecidos após o tratamento com CA ou CAPE em ratos, sugerindo que estes compostos devem ser considerados agentes com potencial terapêutico em doenças imunes, vasculares e neurológicas relacionadas com o sistema colinérgico e purinérgico.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaSchetinger, Maria Rosa ChitolinaBraganhol, Elizandrahttp://lattes.cnpq.br/3081112950297594Athayde, Margareth Lindehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707843T7Leal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Soares, Félix Alexandre Antuneshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769181A8Anwar, Javed2013-05-142013-05-142013-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfANWAR, Javed. THE EFFECTS OF CAFFEIC ACID AND CAFFEIC ACID PHENETHYL ESTER ON THE ACTIVITIES OF ACETYLCHOLINESTERASE AND ECTO-NUCLEOTIDASES IN RATS. 2013. 162 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/4459ark:/26339/001300000k08fporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-25T14:07:20Zoai:repositorio.ufsm.br:1/4459Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T14:07:20Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
THE EFFECTS OF CAFFEIC ACID AND CAFFEIC ACID PHENETHYL ESTER ON THE ACTIVITIES OF ACETYLCHOLINESTERASE AND ECTO-NUCLEOTIDASES IN RATS
title Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
spellingShingle Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
Anwar, Javed
Acetilcolinesterase
Acetilcolina
Nucleotídeo de adenina
Ectonucleotidases
Purinoreceptores
Ácido cafeico
Éster fenetil do ácido cafeico
Actylcholinesterase
Actylcholine
Adenin nucleotides
Ecto-nucleotidases
Purinoreceptors
Caffeic acid
Caffeic acid phenethyl ester
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
title_full Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
title_fullStr Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
title_full_unstemmed Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
title_sort Os efeitos do ácido cafeico e do éster fenetil do ácido cafeico sobre as atividades da acetilcolinesterase e das ecto-nucleotidases em ratos
author Anwar, Javed
author_facet Anwar, Javed
author_role author
dc.contributor.none.fl_str_mv Schetinger, Maria Rosa Chitolina
Braganhol, Elizandra
http://lattes.cnpq.br/3081112950297594
Athayde, Margareth Linde
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707843T7
Leal, Daniela Bitencourt Rosa
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7
Soares, Félix Alexandre Antunes
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769181A8
dc.contributor.author.fl_str_mv Anwar, Javed
dc.subject.por.fl_str_mv Acetilcolinesterase
Acetilcolina
Nucleotídeo de adenina
Ectonucleotidases
Purinoreceptores
Ácido cafeico
Éster fenetil do ácido cafeico
Actylcholinesterase
Actylcholine
Adenin nucleotides
Ecto-nucleotidases
Purinoreceptors
Caffeic acid
Caffeic acid phenethyl ester
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Acetilcolinesterase
Acetilcolina
Nucleotídeo de adenina
Ectonucleotidases
Purinoreceptores
Ácido cafeico
Éster fenetil do ácido cafeico
Actylcholinesterase
Actylcholine
Adenin nucleotides
Ecto-nucleotidases
Purinoreceptors
Caffeic acid
Caffeic acid phenethyl ester
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Phenolic compouds and their derivatives constitute a leading family of natural compounds. Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) are the important members of phenolic compound, sharing several biological applications; antioxidant, neuroprotective, anti-inflammatory, antiproliferative, antibacterial, antiviral, antiatherosclerotic and anticancer properties. In spite of these, literature reportes some of its pro-oxidants activity depending on cellular environment. These pathophysiolocal properties increased the interest to evaluate the effect of CA and CAPE on the enzyme evolved in the purines salvage and the acetylcholine hydrolyzing enzyme the acetylcholineterase (AChE); in both PNS and CNS, since the essential constituent of our dietary items. Previously, our research group has reported that phenolic compound altered the activities of these enzymes. The AChE rapidly hydrolyzes the acetylcholine in neuronal and non neuronal tissues, mediating several neurodegenerative diseases. Beside the ACh, ATP (as co-neurotramittors) and adenosine are important signaling molecules, communicating cells in both PNS and CNS. In the extracellular signalling pathways; the adenine nucleotides, their derivative and the coupling of these molecules with specific receptor have a crucial role in nervous, vascular and immune systems. Once released, these molecules are hydrolyzed by a cascade of enzymes including ectonucleoside triphosphate diphosphohydrolase (NTPDase; E.C. 3.6.1.5, CD39), 5 -nucleotidase (E.C 3.1.3.5, CD73), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), modulating crucially the signaling pathways in the normal functioning of nervous, vascular and immune systems. Next, adenosine deaminase (ADA) and xanthin oxidase (XO) degrade the adenosine and xanthine respectively which further control the functioning mechanisms in cellular events. Found in the neuronal and non neuronal (in both PNS and CNS) the AChE, NTPDase, 5'-nucleotidase, E-NPP, and ADA regulate several events including neurotransmission, inflammation, and thrombogenic process. We hypothesized to evaluate first the in vitro effects of CA on AChE activity in peripheral and central cholinergic system of rats. The results showed that CA significantly modulated the cholinergic system in vitro. By modulating the cholinergic system in vitro, apparently CA (e.g. phenolic structure) has proper role in neurotransmission. Therefore we hypothesizes to evaluate the in vivo effects of CA on AChE, NTPDase, E-NPP, 5'-nucleotidase, platelets aggregation and (ADA) in different tissues/cell from rats. The animals were treated during 30 days and killed after behavioral test. The results showed that caffeic acid increased significantly the AChE activity in hippocampus, hyphothalmus, pon and lymphocytes while that in cortex, cerebellum and striatum the AChE was inhibited. CA improves step-down latencies in the inhibitory avoidance. Investigating the in vivo effects of CA in purinergic system, caffecid acid increased the ATP and AMP hydrolysis in synaptosomes. However, in the synaptosomes no alterations were observed in the ADA activity in the groups evaluated in this study. CA increased the ATP and AMP hydrolysis, while the ADP hydrolysis was decreased in platelets. In the present findings caffeic acid decreased the platelets aggregation induced by ADP agonist. Treatment with CA also increased the NTPDase and ADA activities in lymphocytes of rats. Considering the dual function of caffeic acid in vitro and in vivo, the present study was extended to CAPE followed by acute treatment model (ip) in order to elucidate the effect of another phenolic structure on the same parameters. In this line the animals were treated (ip) with CAPE and killed after 40 minutes. In platelets, the results showed that the effect of CAPE increased the NTPDase, E-NPP, 5 -nucleotidase activities, while ADA activities did not change significantly. In synaptosomes CAPE significantly inhibited the NTPDase, and 5 -nucleotidase activity. CAPE induced no significant changes in ADA in synaptosomes but reduced XO in whole brain. Finaly we investigated the activity of AChE in cortex, cerebellum, striatum, hippocampus, hyphotalamus, pon, lymphocytes and muscules of rats treated with CAPE. The results showed that CAPE significantly decreased the AChE activity in cortex cerebellum and striatum. CAPE significantly increased the AChE activity in hippocampus hypothalamus, pons, muscle and lymphocytes. In cholinergic system our results clearly demonstrating that both compound with dual functions.These findings demonstrated that the AChE activities and the cascade of ecto-enzymes was alter in different tissues after treatment with CA and CAPE in rats, suggesting that these compound should be considered a potentially therapeutic agent in immune, vascular and neurological disorders related with the cholinergic purinergic system.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-14
2013-05-14
2013-01-21
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv ANWAR, Javed. THE EFFECTS OF CAFFEIC ACID AND CAFFEIC ACID PHENETHYL ESTER ON THE ACTIVITIES OF ACETYLCHOLINESTERASE AND ECTO-NUCLEOTIDASES IN RATS. 2013. 162 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013.
http://repositorio.ufsm.br/handle/1/4459
dc.identifier.dark.fl_str_mv ark:/26339/001300000k08f
identifier_str_mv ANWAR, Javed. THE EFFECTS OF CAFFEIC ACID AND CAFFEIC ACID PHENETHYL ESTER ON THE ACTIVITIES OF ACETYLCHOLINESTERASE AND ECTO-NUCLEOTIDASES IN RATS. 2013. 162 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013.
ark:/26339/001300000k08f
url http://repositorio.ufsm.br/handle/1/4459
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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