Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5926 |
Resumo: | The aim of this work was the development of nanostructured formulations containing M. alternifolia essential oil. Polymeric nanocapsules and nanoemulsions (10 mg/mL) were prepared by the interfacial deposition of preformed polymer method and spontaneous emulsification, respectively. The formulations were evaluated in relation to their physicochemical characteristics (particle size, polydispersity index, zeta potential, pH and oil content). Formulations presented oil content of about 96%, nanometric mean size (160-220 nm), polydispersity index below 0.25 and negative zeta potential. The pH values were 6.43 ± 0.37 and 5.98 ± 0.00 for nanoemulsions and nanocapsules, respectively. The inclusion of oil in nanocapsules showed higher protection against volatilization. The analysis of mean size and polydispersity index of the formulations presented no significant alteration during 60 days of storage time (at 25 °C, protected from light). The in vitro antifungal activity of the formulations was evaluated through the study of the inhibition growth profile of Candida albicans yeast. In addition, the antifungal activity against Trichophyton rubrum was studied using two different in vitro models of dermatophyte nail infection. The effect of nanostructures on the growth curve of C. albicans showed inhibition and control of its growing. Nail infection models demonstrated the ability of the formulations in reducing the T. rubrum growth, and the inclusion of oil in nanocapsules was more efficient. The development of hydrogels containing the nanostructured systems were carried out. The semisolid formulations showed adequate properties for topical administration. The topical antiedematogenic effect was evaluated by ear thickness measurement before and 24 h after UVB radiation. Hydrogels containing the colloidal systems showed effect, unlike to hydrogel containing the free oil. The in vivo healing effect of hydrogels was evaluated by regression of the wound in the rat skin. Hydrogel containing the nanocapsules presented higher activity in comparison to the positive control (hydrogel containing allantoin). Analyses of the oxidative stress showed an increase of glutathione, vitamin C and catalase levels after the treatment with the hydrogel containing the nanocapsules prepared with melaleuca essential oil. |
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2013-05-132013-05-132011-08-15FLORES, Fernanda Cramer. NANOSTRUCTURED SYSTEMS CONTAINING Melaleuca alternifolia ESSENTIAL OIL: FORMULATIONS DEVELOPMENT AND BIOLOGICAL ACTIVITY. 2011. 114 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/5926The aim of this work was the development of nanostructured formulations containing M. alternifolia essential oil. Polymeric nanocapsules and nanoemulsions (10 mg/mL) were prepared by the interfacial deposition of preformed polymer method and spontaneous emulsification, respectively. The formulations were evaluated in relation to their physicochemical characteristics (particle size, polydispersity index, zeta potential, pH and oil content). Formulations presented oil content of about 96%, nanometric mean size (160-220 nm), polydispersity index below 0.25 and negative zeta potential. The pH values were 6.43 ± 0.37 and 5.98 ± 0.00 for nanoemulsions and nanocapsules, respectively. The inclusion of oil in nanocapsules showed higher protection against volatilization. The analysis of mean size and polydispersity index of the formulations presented no significant alteration during 60 days of storage time (at 25 °C, protected from light). The in vitro antifungal activity of the formulations was evaluated through the study of the inhibition growth profile of Candida albicans yeast. In addition, the antifungal activity against Trichophyton rubrum was studied using two different in vitro models of dermatophyte nail infection. The effect of nanostructures on the growth curve of C. albicans showed inhibition and control of its growing. Nail infection models demonstrated the ability of the formulations in reducing the T. rubrum growth, and the inclusion of oil in nanocapsules was more efficient. The development of hydrogels containing the nanostructured systems were carried out. The semisolid formulations showed adequate properties for topical administration. The topical antiedematogenic effect was evaluated by ear thickness measurement before and 24 h after UVB radiation. Hydrogels containing the colloidal systems showed effect, unlike to hydrogel containing the free oil. The in vivo healing effect of hydrogels was evaluated by regression of the wound in the rat skin. Hydrogel containing the nanocapsules presented higher activity in comparison to the positive control (hydrogel containing allantoin). Analyses of the oxidative stress showed an increase of glutathione, vitamin C and catalase levels after the treatment with the hydrogel containing the nanocapsules prepared with melaleuca essential oil.O principal objetivo deste trabalho foi o desenvolvimento de formulações nanoestruturadas contendo o óleo essencial de Melaleuca alternifolia. Suspensões de nanocápsulas poliméricas e nanoemulsões (10 mg/mL) foram preparadas pelos métodos de deposição interfacial de polímero pré-formado e emulsificação espontânea, respectivamente, e avaliadas em relação as suas características físico-químicas (tamanho de partícula, índice de polidispersão, potencial zeta, pH e determinação do conteúdo de óleo). As formulações apresentaram rendimento em óleo de, aproximadamente, 96%, diâmetro médio nanométrico (160-200 nm), índice de polidispersão inferior a 0,25 e potencial zeta negativo. Os valores de pH foram de 6,43 ± 0,37 e 5,98 ± 0,00 para nanoemulsões e suspensões de nanocápsulas, respectivamente. A nanoencapsulação do óleo apresentou uma maior proteção deste frente à volatilização. As análises de tamanho médio de partícula e índice de polidispersão não apresentaram alterações durante 60 dias de armazenamento, a uma temperatura de 25 °C, ao abrigo da luz. A avaliação da atividade antifúngica das formulações foi realizada, através do estudo do perfil de inibição do crescimento da levedura de Candida albicans. Adicionalmente, a atividade antifúngica também foi testada frente ao Trichophyton rubrum, utilizando dois diferentes modelos de infecção ungueal. O efeito das nanoestruturas na curva de crescimento da levedura demonstrou inibição e controle do crescimento. Nos modelos de infecção de unha foi possível evidenciar a habilidade das formulações em reduzir o crescimento do T. rubrum, obtendo-se resultados promissores para as nanocápsulas. O estudo de desenvolvimento de hidrogéis contendo os sistemas nanoestruturados propostos neste trabalho foi, também, realizado. As formulações semissólidas desenvolvidas apresentaram propriedades adequadas para aplicação tópica. Ainda, o efeito antiedematogênico tópico foi avaliado pela medida da espessura da orelha de camundongos antes a após 24 horas de exposição à radiação. Os hidrogéis contendo os sistemas coloidais desenvolvidos demonstraram atividade, ao passo que o gel contendo o óleo livre não apresentou efeito. Adicionalmente, o efeito cicatrizante in vivo dos hidrogéis foi avaliado através da regressão da área da ferida na pele de ratos. No modelo de cicatrização, o hidrogel contendo as nanocápsulas apresentou atividade superior ao controle positivo (hidrogel contendo alantoína). Análises de marcadores de estresse oxidativo demonstraram aumento nos níveis de glutationa, vitamina C e catalase após o tratamento com o hidrogel contendo as nanocápsulas preparadas com o óleo essencial de melaleuca.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRFarmáciaMelaleuca alternifoliaNanocápsulasNanoemulsõesHidrogéisMelaleuca alternifoliaNanocapsulesNanoemulsionsHydrogelsCNPQ::CIENCIAS DA SAUDE::FARMACIASistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológicaNanostructured systems containing Melaleuca alternifolia essential oil: formulations development and biological activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSilva, Cristiane de Bona dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791812Z5Beck, Ruy Carlos Ruverhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705330E7Adams, Andréa Inês Hornhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798946T6http://lattes.cnpq.br/1730645957936098Flores, Fernanda Cramer20100000000040030030030030077eeaef8-05f4-4cee-b949-c09342e7eb61e859f19c-cca6-446d-8c91-1d21657faf62401204e7-1b47-4c95-a7fa-9495b909f502a10c96aa-5845-4f8b-b3d9-bcbe923c48dainfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFLORES, FERNANDA CRAMER.pdfapplication/pdf5830602http://repositorio.ufsm.br/bitstream/1/5926/1/FLORES%2c%20FERNANDA%20CRAMER.pdf263baf596b1325a1d560adfdaf066f3dMD51TEXTFLORES, FERNANDA CRAMER.pdf.txtFLORES, FERNANDA CRAMER.pdf.txtExtracted texttext/plain231011http://repositorio.ufsm.br/bitstream/1/5926/2/FLORES%2c%20FERNANDA%20CRAMER.pdf.txt92093e7b51e185a0128c4de91303f6c6MD52THUMBNAILFLORES, FERNANDA CRAMER.pdf.jpgFLORES, FERNANDA CRAMER.pdf.jpgIM Thumbnailimage/jpeg4860http://repositorio.ufsm.br/bitstream/1/5926/3/FLORES%2c%20FERNANDA%20CRAMER.pdf.jpg74e72e9792a57bf39720c2cd35b4918cMD531/59262022-10-13 15:21:24.076oai:repositorio.ufsm.br:1/5926Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-13T18:21:24Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
dc.title.alternative.eng.fl_str_mv |
Nanostructured systems containing Melaleuca alternifolia essential oil: formulations development and biological activity |
title |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
spellingShingle |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica Flores, Fernanda Cramer Melaleuca alternifolia Nanocápsulas Nanoemulsões Hidrogéis Melaleuca alternifolia Nanocapsules Nanoemulsions Hydrogels CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
title_full |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
title_fullStr |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
title_full_unstemmed |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
title_sort |
Sistemas nanoestruturados contendo óleo essencial de Melaleuca alternifolia: desenvolvimento de formulações e atividade biológica |
author |
Flores, Fernanda Cramer |
author_facet |
Flores, Fernanda Cramer |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Cristiane de Bona da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791812Z5 |
dc.contributor.referee1.fl_str_mv |
Beck, Ruy Carlos Ruver |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705330E7 |
dc.contributor.referee2.fl_str_mv |
Adams, Andréa Inês Horn |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798946T6 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1730645957936098 |
dc.contributor.author.fl_str_mv |
Flores, Fernanda Cramer |
contributor_str_mv |
Silva, Cristiane de Bona da Beck, Ruy Carlos Ruver Adams, Andréa Inês Horn |
dc.subject.por.fl_str_mv |
Melaleuca alternifolia Nanocápsulas Nanoemulsões Hidrogéis |
topic |
Melaleuca alternifolia Nanocápsulas Nanoemulsões Hidrogéis Melaleuca alternifolia Nanocapsules Nanoemulsions Hydrogels CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Melaleuca alternifolia Nanocapsules Nanoemulsions Hydrogels |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
The aim of this work was the development of nanostructured formulations containing M. alternifolia essential oil. Polymeric nanocapsules and nanoemulsions (10 mg/mL) were prepared by the interfacial deposition of preformed polymer method and spontaneous emulsification, respectively. The formulations were evaluated in relation to their physicochemical characteristics (particle size, polydispersity index, zeta potential, pH and oil content). Formulations presented oil content of about 96%, nanometric mean size (160-220 nm), polydispersity index below 0.25 and negative zeta potential. The pH values were 6.43 ± 0.37 and 5.98 ± 0.00 for nanoemulsions and nanocapsules, respectively. The inclusion of oil in nanocapsules showed higher protection against volatilization. The analysis of mean size and polydispersity index of the formulations presented no significant alteration during 60 days of storage time (at 25 °C, protected from light). The in vitro antifungal activity of the formulations was evaluated through the study of the inhibition growth profile of Candida albicans yeast. In addition, the antifungal activity against Trichophyton rubrum was studied using two different in vitro models of dermatophyte nail infection. The effect of nanostructures on the growth curve of C. albicans showed inhibition and control of its growing. Nail infection models demonstrated the ability of the formulations in reducing the T. rubrum growth, and the inclusion of oil in nanocapsules was more efficient. The development of hydrogels containing the nanostructured systems were carried out. The semisolid formulations showed adequate properties for topical administration. The topical antiedematogenic effect was evaluated by ear thickness measurement before and 24 h after UVB radiation. Hydrogels containing the colloidal systems showed effect, unlike to hydrogel containing the free oil. The in vivo healing effect of hydrogels was evaluated by regression of the wound in the rat skin. Hydrogel containing the nanocapsules presented higher activity in comparison to the positive control (hydrogel containing allantoin). Analyses of the oxidative stress showed an increase of glutathione, vitamin C and catalase levels after the treatment with the hydrogel containing the nanocapsules prepared with melaleuca essential oil. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-08-15 |
dc.date.accessioned.fl_str_mv |
2013-05-13 |
dc.date.available.fl_str_mv |
2013-05-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FLORES, Fernanda Cramer. NANOSTRUCTURED SYSTEMS CONTAINING Melaleuca alternifolia ESSENTIAL OIL: FORMULATIONS DEVELOPMENT AND BIOLOGICAL ACTIVITY. 2011. 114 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5926 |
identifier_str_mv |
FLORES, Fernanda Cramer. NANOSTRUCTURED SYSTEMS CONTAINING Melaleuca alternifolia ESSENTIAL OIL: FORMULATIONS DEVELOPMENT AND BIOLOGICAL ACTIVITY. 2011. 114 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/5926 |
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por |
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201000000000 |
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400 300 300 300 300 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Ciências Farmacêuticas |
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UFSM |
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BR |
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Farmácia |
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Universidade Federal de Santa Maria |
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