Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Gianine Lima
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/00130000079zm
Texto Completo: http://repositorio.ufsm.br/handle/1/8977
Resumo: Diabetes mellitus (DM) is a chronic disease characterized by hyperglycemia, which is related to oxidative stress and plays an important role in the development of other diseases and tissue damage, such as liver and kidney damage. Thus, it is important for studies with potential antioxidant that may reduce the deleterious effects of oxidative stress due to diabetes. In this sense, N-acetylcysteine (NAC) is used as a hepatoprotective drug in the treatment of acute poisoning by paracetamol to reduce oxidative damage. Along these lines, the aims of this study were to evaluate biomarkers of oxidative stress such as reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) and delta aminolevulinate acid dehydratase (ALA-D) in liver and kidneys of controls and animals with diabetes induced after treatment with NAC. Treatments consisted of intraperitoneal administration of 25 mg / kg and 75 mg / kg N-acetylcysteine. In the liver, MDA levels were significantly increased in the diabetic group compared to controls, treatment with 75 mg / kg reduced the levels of MDA, being similar to the control group. GSH levels of the enzyme was found to be highest in kidney and liver of diabetic animals than controls, and treatment with NAC led to these reduced levels in the liver of diabetic animals, but in the kidney, no changes. The levels of SOD and GPx decreased in the liver of diabetic animals compared to control, and administration of NAC did not alter these indices. Diabetes also reduced the activity of ALA-D in the liver, and treatment with 25 mg/ kg NAC did with this activity increased significantly. In the kidney, both doses of NAC increased the levels of ALA-D in diabetic animals. The results suggest that NAC may be more effective in the liver, the organ that suffers most oxidative changes, and especially in groups of diabetic animals.
id UFSM_313f1b49358bb40e76dda3e5beab711e
oai_identifier_str oai:repositorio.ufsm.br:1/8977
network_acronym_str UFSM
network_name_str Manancial - Repositório Digital da UFSM
repository_id_str
spelling Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticosTissue oxidative stress of diabetic rats treated with N-acetylcysteineDiabetes mellitusBiomarcadores enzimáticosBiomarcadores não enzimáticosEstresse oxidativoN-acetilcisteínaDiabetes mellitusEnzymatic biomarkersNon enzymatic biomarkersOxidative stressN-acetylcysteineCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIADiabetes mellitus (DM) is a chronic disease characterized by hyperglycemia, which is related to oxidative stress and plays an important role in the development of other diseases and tissue damage, such as liver and kidney damage. Thus, it is important for studies with potential antioxidant that may reduce the deleterious effects of oxidative stress due to diabetes. In this sense, N-acetylcysteine (NAC) is used as a hepatoprotective drug in the treatment of acute poisoning by paracetamol to reduce oxidative damage. Along these lines, the aims of this study were to evaluate biomarkers of oxidative stress such as reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) and delta aminolevulinate acid dehydratase (ALA-D) in liver and kidneys of controls and animals with diabetes induced after treatment with NAC. Treatments consisted of intraperitoneal administration of 25 mg / kg and 75 mg / kg N-acetylcysteine. In the liver, MDA levels were significantly increased in the diabetic group compared to controls, treatment with 75 mg / kg reduced the levels of MDA, being similar to the control group. GSH levels of the enzyme was found to be highest in kidney and liver of diabetic animals than controls, and treatment with NAC led to these reduced levels in the liver of diabetic animals, but in the kidney, no changes. The levels of SOD and GPx decreased in the liver of diabetic animals compared to control, and administration of NAC did not alter these indices. Diabetes also reduced the activity of ALA-D in the liver, and treatment with 25 mg/ kg NAC did with this activity increased significantly. In the kidney, both doses of NAC increased the levels of ALA-D in diabetic animals. The results suggest that NAC may be more effective in the liver, the organ that suffers most oxidative changes, and especially in groups of diabetic animals.O diabetes mellitus (DM) é uma doença crônica caracterizada pela hiperglicemia, que está relacionada Com o estresse oxidativo, o qual possui papel importante no desenvolvimento de outras patologias e danos teciduais, tais como dano hepático e renal. Dessa forma, faz-se importante à realização de estudos com possíveis antioxidantes, que possam diminuir os efeitos deletérios do estresse oxidativo decorrentes do diabetes. Neste sentido, a N-acetilcisteína (NAC) é um medicamento utilizado como hepatoprotetor por estimular a síntese de Glutationa Reduzida, diminuindo o dano oxidativo. Nesta linha, o objetivo deste trabalho foi avaliar o efeito antioxidante da NAC nos tecidos renal e hepático de ratos diabéticos através dos biomarcadores do estresse oxidativo como: glutationa reduzida (GSH), glutationa peroxidase (GPx), superóxido dismutase (SOD), malondialdeído (MDA) e ácido delta aminolevulinato desidratase (ALA-D) no fígado e rins de animais controles e com diabetes induzida, tratados e não tratados com NAC. Os tratamentos consistiram em administrações intraperitoneais de 25 mg/Kg e 75 mg/Kg de N-acetilcisteína. No fígado, os níveis de MDA foram significativamente aumentados no grupo diabético comparados ao grupo controle. O tratamento com 75 mg/Kg foi capaz de reduzir os níveis de MDA, ficando semelhantes ao grupo controle. Os níveis da GSH mostrou-se mais elevada no rim e no fígado dos animais diabéticos do que dos controles, e o tratamento com a NAC fez com que esses níveis fossem reduzidos no fígado dos animais diabéticos, entretanto no rim, não houve alterações. Os níveis de SOD e GPx diminuíram no fígado dos animais diabéticos quando comparados ao controle, e a administração de NAC não alterou esses índices. O diabetes também diminuiu a atividade da ALA-D no fígado, e o tratamento com a 25 mg/Kg NAC fez com essa atividade aumentasse significativamente. No tecido renal, ambas as doses de NAC elevaram os níveis de ALA-D nos animais diabéticos. Diante dos resultados encontrados, comparando-se os tecidos renal e hepático dos ratos controles com os diabéticos tratados com NAC, sugere-se que a NAC demonstrou diminuir o dano oxidativo mais no fígado do que no rim.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaGarcia, Solange Cristinahttp://lattes.cnpq.br/6687355709603379Beck, Ruy Carlos Ruverhttp://lattes.cnpq.br/5548361916821654Moretto, Maria Beatrizhttp://lattes.cnpq.br/7317262818918502Ribeiro, Gianine Lima2013-05-202013-05-202010-10-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfRIBEIRO, Gianine Lima. Tissue oxidative stress of diabetic rats treated with N-acetylcysteine. 2010. 67 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/8977ark:/26339/00130000079zmporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-13T11:19:20Zoai:repositorio.ufsm.br:1/8977Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:27:47.669420Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
Tissue oxidative stress of diabetic rats treated with N-acetylcysteine
title Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
spellingShingle Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
Ribeiro, Gianine Lima
Diabetes mellitus
Biomarcadores enzimáticos
Biomarcadores não enzimáticos
Estresse oxidativo
N-acetilcisteína
Diabetes mellitus
Enzymatic biomarkers
Non enzymatic biomarkers
Oxidative stress
N-acetylcysteine
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
title_full Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
title_fullStr Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
title_full_unstemmed Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
title_sort Efeitos da N-acetilcisteína sobre o dano oxidativo renal e hepático de ratos diabéticos
author Ribeiro, Gianine Lima
author_facet Ribeiro, Gianine Lima
author_role author
dc.contributor.none.fl_str_mv Garcia, Solange Cristina
http://lattes.cnpq.br/6687355709603379
Beck, Ruy Carlos Ruver
http://lattes.cnpq.br/5548361916821654
Moretto, Maria Beatriz
http://lattes.cnpq.br/7317262818918502
dc.contributor.author.fl_str_mv Ribeiro, Gianine Lima
dc.subject.por.fl_str_mv Diabetes mellitus
Biomarcadores enzimáticos
Biomarcadores não enzimáticos
Estresse oxidativo
N-acetilcisteína
Diabetes mellitus
Enzymatic biomarkers
Non enzymatic biomarkers
Oxidative stress
N-acetylcysteine
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
topic Diabetes mellitus
Biomarcadores enzimáticos
Biomarcadores não enzimáticos
Estresse oxidativo
N-acetilcisteína
Diabetes mellitus
Enzymatic biomarkers
Non enzymatic biomarkers
Oxidative stress
N-acetylcysteine
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description Diabetes mellitus (DM) is a chronic disease characterized by hyperglycemia, which is related to oxidative stress and plays an important role in the development of other diseases and tissue damage, such as liver and kidney damage. Thus, it is important for studies with potential antioxidant that may reduce the deleterious effects of oxidative stress due to diabetes. In this sense, N-acetylcysteine (NAC) is used as a hepatoprotective drug in the treatment of acute poisoning by paracetamol to reduce oxidative damage. Along these lines, the aims of this study were to evaluate biomarkers of oxidative stress such as reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) and delta aminolevulinate acid dehydratase (ALA-D) in liver and kidneys of controls and animals with diabetes induced after treatment with NAC. Treatments consisted of intraperitoneal administration of 25 mg / kg and 75 mg / kg N-acetylcysteine. In the liver, MDA levels were significantly increased in the diabetic group compared to controls, treatment with 75 mg / kg reduced the levels of MDA, being similar to the control group. GSH levels of the enzyme was found to be highest in kidney and liver of diabetic animals than controls, and treatment with NAC led to these reduced levels in the liver of diabetic animals, but in the kidney, no changes. The levels of SOD and GPx decreased in the liver of diabetic animals compared to control, and administration of NAC did not alter these indices. Diabetes also reduced the activity of ALA-D in the liver, and treatment with 25 mg/ kg NAC did with this activity increased significantly. In the kidney, both doses of NAC increased the levels of ALA-D in diabetic animals. The results suggest that NAC may be more effective in the liver, the organ that suffers most oxidative changes, and especially in groups of diabetic animals.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-22
2013-05-20
2013-05-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, Gianine Lima. Tissue oxidative stress of diabetic rats treated with N-acetylcysteine. 2010. 67 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
http://repositorio.ufsm.br/handle/1/8977
dc.identifier.dark.fl_str_mv ark:/26339/00130000079zm
identifier_str_mv RIBEIRO, Gianine Lima. Tissue oxidative stress of diabetic rats treated with N-acetylcysteine. 2010. 67 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
ark:/26339/00130000079zm
url http://repositorio.ufsm.br/handle/1/8977
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
_version_ 1814439745755807744