Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes

Detalhes bibliográficos
Autor(a) principal: Oliveira, Lizielle Souza de
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000p0hf
Texto Completo: http://repositorio.ufsm.br/handle/1/17353
Resumo: Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia associated with the increase of free radicals and decrease in the antioxidant response. Gallic acid has many biological properties, among them we can highlight its antioxidant function that can provide a number of benefits for patients with DM. In this context, the aim of this study was to evaluate the treatment effect of gallic acid in biochemical parameters of oxidative stress and histological biomarkers in liver and kidney of diabetic rats induced with streptozotocin. In the present study, we used 40 male Wistar rats were divided into four groups: I - Control II - gallic acid (30mg / kg), III - diabetic IV - Diabetic + gallic acid (30mg / kg). Diabetes mellitus was induced in the animals by intraperitoneal injection of streptozotocin (65 mg / kg). After confirmation of hyperglycemia, began treatment with gallic acid (30mg / kg) which was administered orally for 21 days. After this period the animals were euthanized and blood, liver and kidney were collected for further analysis. The results demonstrated that treatment with gallic acid was able to prevent the formation of reactive species to reduce the levels of lipid peroxidation and increase the activity of superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of diabetic rats (P < 0.05) Further, this compound also prevented the decrease in activity of superoxide dismutase, glutathione S-transferase and Vitamin C levels in the livers of diabetic mice (P <0.05). Gallic acid treatment prevented the increase of aspartate aminotransferase activity in liver tissue and reduced the number of nuclei and increased nuclear area, and increased glomerular renal tissue area. These results indicate that the gallic acid may protect against oxidative stress-induced damage in the diabetic state. Therefore, it can be suggested that the gallic acid may be a potential candidate in adjunct therapy in diabetes, due to its antioxidant action, in combination with hypoglycemic drugs.
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spelling Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetesEffects of gallic acid on biochemical parameters and oxidative stress in rats submitted to the experimental model of diabetesAntioxidantesÁcido gálicoDelta-aminolevulinato ácido desidrataseDiabetes mellitusAntioxidantDelta-aminolevulinic acid dehydrataseGallic acidCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICADiabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia associated with the increase of free radicals and decrease in the antioxidant response. Gallic acid has many biological properties, among them we can highlight its antioxidant function that can provide a number of benefits for patients with DM. In this context, the aim of this study was to evaluate the treatment effect of gallic acid in biochemical parameters of oxidative stress and histological biomarkers in liver and kidney of diabetic rats induced with streptozotocin. In the present study, we used 40 male Wistar rats were divided into four groups: I - Control II - gallic acid (30mg / kg), III - diabetic IV - Diabetic + gallic acid (30mg / kg). Diabetes mellitus was induced in the animals by intraperitoneal injection of streptozotocin (65 mg / kg). After confirmation of hyperglycemia, began treatment with gallic acid (30mg / kg) which was administered orally for 21 days. After this period the animals were euthanized and blood, liver and kidney were collected for further analysis. The results demonstrated that treatment with gallic acid was able to prevent the formation of reactive species to reduce the levels of lipid peroxidation and increase the activity of superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of diabetic rats (P < 0.05) Further, this compound also prevented the decrease in activity of superoxide dismutase, glutathione S-transferase and Vitamin C levels in the livers of diabetic mice (P <0.05). Gallic acid treatment prevented the increase of aspartate aminotransferase activity in liver tissue and reduced the number of nuclei and increased nuclear area, and increased glomerular renal tissue area. These results indicate that the gallic acid may protect against oxidative stress-induced damage in the diabetic state. Therefore, it can be suggested that the gallic acid may be a potential candidate in adjunct therapy in diabetes, due to its antioxidant action, in combination with hypoglycemic drugs.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESO diabetes mellitus (DM) é uma doença metabólica caracterizada por hiperglicemia associada com o aumento de radicais livres e diminuição da resposta antioxidante. O ácido gálico tem inúmeras propriedades biológicas, dentre elas pode-se destacar sua função antioxidante que pode fornecer uma série de benefícios para os pacientes com DM. Neste contexto, o objetivo deste estudo foi avaliar o efeito do tratamento com ácido gálico em parâmetros bioquímicos, biomarcadores de estresse oxidativo e histológicos em fígado e rim de ratos diabéticos induzidos com estreptozotocina. No presente estudo, foram utilizados 40 ratos wistar machos que foram divididos em quatro grupos: I - controle, II - ácido gálico (30mg/kg), III - diabético, IV - diabético + ácido gálico (30mg/kg). O DM foi induzido nos animais através de injeção intraperitoneal de estreptozotocina (65 mg/kg). Após a confirmação da hiperglicemia, iniciou-se o tratamento com ácido gálico (30mg/kg) que foi administrado por via oral durante 21 dias. Após este período os animais foram submetidos à eutanásia e o sangue, fígado e rim foram coletados para as posteriores análises. Os resultados obtidos demonstraram que o tratamento com ácido gálico foi capaz de prevenir a formação de espécies reativas, reduzir os níveis de peroxidação lipídica e aumentar a atividade das enzimas superóxido dismutase e delta-aminolevulinato ácido desidratase no fígado e rim de ratos diabéticos (P<0.05) Além disso, este composto também preveniu a diminuição na atividade da superóxido dismutase, glutationa S- transferase e dos níveis de vitamina C no fígado de ratos diabéticos (P<0.05). O tratamento com ácido gálico preveniu o aumento da atividade da aspartato aminotransferase e no tecido hepático reduziu o número de núcleos e aumentou a área nuclear, e no tecido renal aumentou a área glomerular. Estes resultados indicam que o ácido gálico pode proteger contra danos induzidos pelo estresse oxidativo no estado diabético. Portanto, pode-se sugerir que o ácido gálico pode ser um candidato potencial na terapia complementar no diabetes, devido às suas ações antioxidantes, em combinação com fármacos hipoglicêmicos.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasSpanevello, Roselia Mariahttp://lattes.cnpq.br/3446031341157893Schmatz, Robertahttp://lattes.cnpq.br/5100771532109977Loro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Oliveira, Lizielle Souza de2019-07-08T20:58:08Z2019-07-08T20:58:08Z2015-09-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/17353ark:/26339/001300000p0hfporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-07-09T06:00:34Zoai:repositorio.ufsm.br:1/17353Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-07-09T06:00:34Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
Effects of gallic acid on biochemical parameters and oxidative stress in rats submitted to the experimental model of diabetes
title Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
spellingShingle Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
Oliveira, Lizielle Souza de
Antioxidantes
Ácido gálico
Delta-aminolevulinato ácido desidratase
Diabetes mellitus
Antioxidant
Delta-aminolevulinic acid dehydratase
Gallic acid
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
title_full Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
title_fullStr Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
title_full_unstemmed Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
title_sort Efeitos do ácido gálico em parâmetros bioquímicos e de estresse oxidativo em ratos submetidos a um modelo experimental de diabetes
author Oliveira, Lizielle Souza de
author_facet Oliveira, Lizielle Souza de
author_role author
dc.contributor.none.fl_str_mv Spanevello, Roselia Maria
http://lattes.cnpq.br/3446031341157893
Schmatz, Roberta
http://lattes.cnpq.br/5100771532109977
Loro, Vania Lucia
http://lattes.cnpq.br/6392817606416780
dc.contributor.author.fl_str_mv Oliveira, Lizielle Souza de
dc.subject.por.fl_str_mv Antioxidantes
Ácido gálico
Delta-aminolevulinato ácido desidratase
Diabetes mellitus
Antioxidant
Delta-aminolevulinic acid dehydratase
Gallic acid
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Antioxidantes
Ácido gálico
Delta-aminolevulinato ácido desidratase
Diabetes mellitus
Antioxidant
Delta-aminolevulinic acid dehydratase
Gallic acid
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia associated with the increase of free radicals and decrease in the antioxidant response. Gallic acid has many biological properties, among them we can highlight its antioxidant function that can provide a number of benefits for patients with DM. In this context, the aim of this study was to evaluate the treatment effect of gallic acid in biochemical parameters of oxidative stress and histological biomarkers in liver and kidney of diabetic rats induced with streptozotocin. In the present study, we used 40 male Wistar rats were divided into four groups: I - Control II - gallic acid (30mg / kg), III - diabetic IV - Diabetic + gallic acid (30mg / kg). Diabetes mellitus was induced in the animals by intraperitoneal injection of streptozotocin (65 mg / kg). After confirmation of hyperglycemia, began treatment with gallic acid (30mg / kg) which was administered orally for 21 days. After this period the animals were euthanized and blood, liver and kidney were collected for further analysis. The results demonstrated that treatment with gallic acid was able to prevent the formation of reactive species to reduce the levels of lipid peroxidation and increase the activity of superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of diabetic rats (P < 0.05) Further, this compound also prevented the decrease in activity of superoxide dismutase, glutathione S-transferase and Vitamin C levels in the livers of diabetic mice (P <0.05). Gallic acid treatment prevented the increase of aspartate aminotransferase activity in liver tissue and reduced the number of nuclei and increased nuclear area, and increased glomerular renal tissue area. These results indicate that the gallic acid may protect against oxidative stress-induced damage in the diabetic state. Therefore, it can be suggested that the gallic acid may be a potential candidate in adjunct therapy in diabetes, due to its antioxidant action, in combination with hypoglycemic drugs.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-11
2019-07-08T20:58:08Z
2019-07-08T20:58:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/17353
dc.identifier.dark.fl_str_mv ark:/26339/001300000p0hf
url http://repositorio.ufsm.br/handle/1/17353
identifier_str_mv ark:/26339/001300000p0hf
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Centro de Ciências Naturais e Exatas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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