Atividade cicatrizante do cubiu (Solanum sessiliflorum) e do campo magnético

• Autor(a) principal: Dalenogare, Jéssica Franco
• Data de Publicação: 2022
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• Texto Completo: http://repositorio.ufsm.br/handle/1/25795
• topic: Maná-cubiu; Cocona; Cicatrização de pele; Reparo tecidual; Skin wound healing; Tissue repair; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA

Skin healing is a complex and distinct process, in which treatments aimed at favoring and accelerating it, such as the therapeutic use of magnetic fields, are beneficial. However, the role of magnetic fields in the oxidative balance and in the systemic inflammatory profile is still controversial in the literature. Given this, it is suggested to use them together with antioxidants. Among these, natural antioxidants from plants, such as the herbaceous nightshade Solanum sessiliflorum (cubiu), stand out. Cubiu is widely used in traditional medicine not only as an antioxidant, but also with claims of hypoglycemic and hypolipidemic properties, in the fight against diabetes and for skin healing. However, despite its popular use, there are few studies investigating the safety of its use in terms of toxicity, elucidating its real action in the oxidative profile, as well as, there are still no data to confirm its anti-inflammatory and healing effect. Thus, the present study aims to investigate the pharmacological activity of cubiu (toxicity, antimicrobial, antioxidant, anti-inflammatory activity) and its healing action against skin wounds and in association with the magnetic field. For this, a first in vitro study was conducted investigating the antimicrobial activity of cubiu extract against the strains of Aeromonas caviae, Pseudomonas aeruginosa (PA01) and Sphingomonas paucimobilis, as well as the inhibition and destruction of the PA01 biofilm. Furthermore, this study evaluated the healing activity of cubiu extract, anti-inflammatory activity and safety profile, using human fibroblast cells HFF1 and human peripheral blood mononuclear cells. In this study, it was found that cubiu has healing activity, with the modulation of the inflammatory response as a mechanism of action, acting as an anti-inflammatory, in addition to demonstrating that it is antimicrobial against the strains tested, inhibiting and destroying the PA01 biofilm and demonstrating safety for the use. From this, the next study will continue the investigation of the healing activity of the cubiu in the face of a skin healing process and in association with the magnetic field, using Wistar rats. First, a protocol is conducted that evaluates the toxicity of a dose-response curve of cubiu in vivo, thus determining the ideal dose. As a sequence, the study of skin healing is presented, where the antioxidant and anti-inflammatory activities of the two treatments alone and in association (cubiu and magnetic field) are evaluated. In this study, it was possible to show that cubiu did not present toxicity at any of the concentrations tested, and that both treatments act as antioxidants and anti-inflammatory agents against the skin healing process, with an increased response when used in association. In conclusion, from the pharmacological screening carried out, this is the first study to prove that cubiu extract is an important compound against skin diseases, promoting skin wound healing, antimicrobial and anti-inflammatory activity.