Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite

Detalhes bibliográficos
Autor(a) principal: Sturza, Diego Artemio Franco
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/00130000032nq
Texto Completo: http://repositorio.ufsm.br/handle/1/10100
Resumo: The success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals.
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spelling Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leitePerformance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infectionAnimais persistentemente infectadosBVDVDiagnósticoAnimals persistently infectedBVDVDiagnosticCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals.Conselho Nacional de Desenvolvimento Científico e TecnológicoO sucesso nas estratégias de controle e erradicação do vírus da diarréia viral bovina (BVDV), passa pela identificação e eliminação dos animais persistentemente infectados (PI). Como esses animais excretam continuamente o vírus, a prevalência e os títulos de anticorpos nos rebanhos que os possuem são geralmente altos. Por isso, amostras de tanques coletivos de leite são frequentemente utilizadas na triagem de rebanhos. No presente trabalho, amostras coletivas de leite e amostras de soro foram submetidas a duas técnicas sorológicas, a fim de estabelecer a mais adequada para esta finalidade. Para tal, 767 amostras coletivas de leite e 701 amostras de soro bovino foram testadas com um kit ELISA indireto (teste padrão) e pela técnica de vírus-neutralização (VNT). Devido aos efeitos tóxicos do leite sobre o cultivo celular, a VNT foi adaptada, o que consistiu no aumento do volume final na etapa de incubação com os cultivos celulares. No teste do leite, foram positivas 177 e 139 amostras, no ELISA e na VNT, respectivamente. Com isso, a VNT adaptada apresentou uma sensibilidade (Se) de 76,8% e uma especificidade (Sp) de 99,5%. O índice Kappa (k) foi de 0,82, demonstrando uma ótima concordância entre as duas técnicas. A análise do coeficiente de correlação entre os valores de absorbância no ELISA (OD) e os títulos de anticorpos na VNT nas amostras positivas, demonstrou uma correlação moderada positiva (r = 0,57) com p<0,05. No teste do soro, foram positivas 442 e 602 amostras no ELISA (teste padrão) e na VNT, respectivamente. Observou-se uma (Se) de 92,7% e (Sp) de 27,1%, com (k) de 0,23, demonstrando uma fraca concordância entre as duas técnicas. O coeficiente de correlação entre OD e os títulos de anticorpos demonstrou uma correlação moderada positiva (r = 0,54) com p<0,05. Observaram-se nos testes do leite e do soro, várias amostras com títulos altos na VNT apresentando ODs baixas a medianas. Por outro lado, várias amostras com títulos neutralizantes baixos apresentaram ODs altas. Baseado nos resultados, e partindo do pressuposto que a presença de animais PI reflete-se em títulos neutralizantes geralmente ≥ 80, conclui-se que a VNT é mais adequada para a realização de triagem em amostras coletivas de leite quando objetiva-se detectar rebanhos com altos títulos de anticorpos neutralizantes, possíveis portadores de animais PI.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaFlores, Eduardo Furtadohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785140A1Weiblen, Rudihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783394D5Scherer, Charles Fernando Capinoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767498P7Sturza, Diego Artemio Franco2017-06-012017-06-012011-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSTURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/10100ark:/26339/00130000032nqporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-25T15:01:41Zoai:repositorio.ufsm.br:1/10100Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T15:01:41Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection
title Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
spellingShingle Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
Sturza, Diego Artemio Franco
Animais persistentemente infectados
BVDV
Diagnóstico
Animals persistently infected
BVDV
Diagnostic
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
title_full Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
title_fullStr Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
title_full_unstemmed Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
title_sort Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
author Sturza, Diego Artemio Franco
author_facet Sturza, Diego Artemio Franco
author_role author
dc.contributor.none.fl_str_mv Flores, Eduardo Furtado
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785140A1
Weiblen, Rudi
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783394D5
Scherer, Charles Fernando Capinos
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767498P7
dc.contributor.author.fl_str_mv Sturza, Diego Artemio Franco
dc.subject.por.fl_str_mv Animais persistentemente infectados
BVDV
Diagnóstico
Animals persistently infected
BVDV
Diagnostic
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Animais persistentemente infectados
BVDV
Diagnóstico
Animals persistently infected
BVDV
Diagnostic
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description The success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-02
2017-06-01
2017-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv STURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.
http://repositorio.ufsm.br/handle/1/10100
dc.identifier.dark.fl_str_mv ark:/26339/00130000032nq
identifier_str_mv STURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.
ark:/26339/00130000032nq
url http://repositorio.ufsm.br/handle/1/10100
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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