Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/00130000032nq |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10100 |
Resumo: | The success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals. |
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Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leitePerformance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infectionAnimais persistentemente infectadosBVDVDiagnósticoAnimals persistently infectedBVDVDiagnosticCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals.Conselho Nacional de Desenvolvimento Científico e TecnológicoO sucesso nas estratégias de controle e erradicação do vírus da diarréia viral bovina (BVDV), passa pela identificação e eliminação dos animais persistentemente infectados (PI). Como esses animais excretam continuamente o vírus, a prevalência e os títulos de anticorpos nos rebanhos que os possuem são geralmente altos. Por isso, amostras de tanques coletivos de leite são frequentemente utilizadas na triagem de rebanhos. No presente trabalho, amostras coletivas de leite e amostras de soro foram submetidas a duas técnicas sorológicas, a fim de estabelecer a mais adequada para esta finalidade. Para tal, 767 amostras coletivas de leite e 701 amostras de soro bovino foram testadas com um kit ELISA indireto (teste padrão) e pela técnica de vírus-neutralização (VNT). Devido aos efeitos tóxicos do leite sobre o cultivo celular, a VNT foi adaptada, o que consistiu no aumento do volume final na etapa de incubação com os cultivos celulares. No teste do leite, foram positivas 177 e 139 amostras, no ELISA e na VNT, respectivamente. Com isso, a VNT adaptada apresentou uma sensibilidade (Se) de 76,8% e uma especificidade (Sp) de 99,5%. O índice Kappa (k) foi de 0,82, demonstrando uma ótima concordância entre as duas técnicas. A análise do coeficiente de correlação entre os valores de absorbância no ELISA (OD) e os títulos de anticorpos na VNT nas amostras positivas, demonstrou uma correlação moderada positiva (r = 0,57) com p<0,05. No teste do soro, foram positivas 442 e 602 amostras no ELISA (teste padrão) e na VNT, respectivamente. Observou-se uma (Se) de 92,7% e (Sp) de 27,1%, com (k) de 0,23, demonstrando uma fraca concordância entre as duas técnicas. O coeficiente de correlação entre OD e os títulos de anticorpos demonstrou uma correlação moderada positiva (r = 0,54) com p<0,05. Observaram-se nos testes do leite e do soro, várias amostras com títulos altos na VNT apresentando ODs baixas a medianas. Por outro lado, várias amostras com títulos neutralizantes baixos apresentaram ODs altas. Baseado nos resultados, e partindo do pressuposto que a presença de animais PI reflete-se em títulos neutralizantes geralmente ≥ 80, conclui-se que a VNT é mais adequada para a realização de triagem em amostras coletivas de leite quando objetiva-se detectar rebanhos com altos títulos de anticorpos neutralizantes, possíveis portadores de animais PI.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaFlores, Eduardo Furtadohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785140A1Weiblen, Rudihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783394D5Scherer, Charles Fernando Capinoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767498P7Sturza, Diego Artemio Franco2017-06-012017-06-012011-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSTURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/10100ark:/26339/00130000032nqporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-25T15:01:41Zoai:repositorio.ufsm.br:1/10100Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T15:01:41Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection |
title |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
spellingShingle |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite Sturza, Diego Artemio Franco Animais persistentemente infectados BVDV Diagnóstico Animals persistently infected BVDV Diagnostic CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
title_full |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
title_fullStr |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
title_full_unstemmed |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
title_sort |
Testes de elisa e vírus-neutralização na detecção de anticorpos contra o vírus da diarréia viral bovina no soro e leite |
author |
Sturza, Diego Artemio Franco |
author_facet |
Sturza, Diego Artemio Franco |
author_role |
author |
dc.contributor.none.fl_str_mv |
Flores, Eduardo Furtado http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785140A1 Weiblen, Rudi http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783394D5 Scherer, Charles Fernando Capinos http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767498P7 |
dc.contributor.author.fl_str_mv |
Sturza, Diego Artemio Franco |
dc.subject.por.fl_str_mv |
Animais persistentemente infectados BVDV Diagnóstico Animals persistently infected BVDV Diagnostic CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Animais persistentemente infectados BVDV Diagnóstico Animals persistently infected BVDV Diagnostic CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The success of control/eradication programs of bovine viral diarrhea virus (BVDV) infection necessarily includes the identification and elimination of persistently infected (PI) animals. Since these animals continuously shed virus, the prevalence and titers of antibodies in herds with PI animals are often high. Hence, bulk milk and serum samples were subjected to two serological tests in order to establish the most appropriate for herd screening. For this, 767 samples of bulk milk and 701 serum samples were analyzed by a commercial ELISA kit and by virus-neutralization test (VNT). The toxic effects of milk for cell cultures on VNT was abolished by increasing the final volume of medium during the incubation. Among the tested milk samples, 177 and 139 were positive in VNT and ELISA, respectively. Thus, the VNT showed a sensitivity (Se) of 76.8% and a specificity (Sp) of 99.5%. The Kappa index (k) was 0.82, demonstrating an excellent agreement between the two techniques. The analysis of the correlation coefficient between the absorbance values in ELISA (OD) and antibody titers in positive samples in VNT showed a moderate positivity (r = 0.57) with p<0.05. In serum samples, 442 and 602 were positive in ELISA and VNT, respectively. This resulted in a VNT sensitivity (Se) of 92.7% and specificity (Sp) of 27.1%, with (k) of 0.23, reflecting a weak agreement between the two techniques. The correlation coefficient between OD and antibody titers showed a moderate positivity (r = 0.54) with p<0.05. Testing of both milk and serum revealed a considerable number of samples with high VN titers and low ODs. On the other side, many samples with low VN titers showed high ODs in the ELISA. Based on the above mentioned results, and assuming that the presence of PI animals usually results in VN titers ≥ 80, we conclude that the VNT is more appropriate to conduct herd screening in bulk milk when the objective is to detect herds with high titers of neutralizing antibodies, potentially harboring PI animals. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-02 2017-06-01 2017-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
STURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/10100 |
dc.identifier.dark.fl_str_mv |
ark:/26339/00130000032nq |
identifier_str_mv |
STURZA, Diego Artemio Franco. Performance of virus neutralizing test and elisa for detection of antibodies and identification of herds with bovine viral diarrhea virus infection. 2011. 42 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. ark:/26339/00130000032nq |
url |
http://repositorio.ufsm.br/handle/1/10100 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1815172272886906880 |