Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares.
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11093 |
Resumo: | The NTPDase1 (E.C. 3.6.1.5, apyrase, CD39, ecto-ATP-diphosphohydrolase) enzymatic activity, which is responsible for hydrolyzing ATP and ADP nucleotides, was verified in lymphocytes from 56 patients under treatment for acute lymphoblastic leukemia (ALL) and from 33 healthy subjects. Patients were divided into 3 groups: remission induction (RI), remission maintenance (RM) and out-oftreatment (OT). It had been verified, in each group, the influence of the recurrence risk (high or low) on the enzymatic activity. The RM group was then subdivided by the amount of time they had been in remission maintenance (0-30, 31-60 and 61-84 weeks). Results demonstrated that the ATP hydrolysis from lymphocyte NTPDase1 is altered in the groups studied (F (3,84)=100.34; p<0.01) in relation to the controls, and that it is reduced in both RI and RM groups, while enhanced in OT group. The recurrence risk did not influence ATP hydrolysis in none of the groups. Furthermore, it has been found a significant variation at the NTPDase1 activity to ATP hydrolysis during RM phase (F (2,17)=11.22; p=0.01), being higher in the first 30 weeks, and subsequently reduced. ADP hydrolysis by NTPDase1 activity was also significantly reduced in both the RI and RM groups, but similar to the controls in OT (F (3,69)=59.05; p<0.01). It has not been verified any influence of the recurrence risk on ADP hydrolysis. Furhermore, NTPDase1 using ADP as substrate has shown to be constant during RM phase (F (2,13)=2.40; p=0.130). The alteration on ATP and ADP hydrolysis on lymphocytes of ALL patients is in agreement to the characteristics found in this pathology. Thus, our results corroborate the role of adenine nucleotides, ATP in special, in the immune system, as signaling molecules, and the importance of the NTPDase1 to maintain constant the extracellular levels of these nucleotides. However, more studies are necessary to better understand the role of adenine nucleotides and NTPDase1 in the immunodeficiency and citotoxicity observed in treated ALL patients. |
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Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares.NTPDase1 activity in lymphocytes from patients under treatment for acute lymphoblastic leukemia (ALL): an alteration in the extracellular ATP and ADP hydrolysisNTPDase1LinfócitosATPADPLeucemia linfoblástica agudaTratamentoNTPDase1LymphocytesATPADPAcute lymphoblastic leukemiaTreatmentCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAThe NTPDase1 (E.C. 3.6.1.5, apyrase, CD39, ecto-ATP-diphosphohydrolase) enzymatic activity, which is responsible for hydrolyzing ATP and ADP nucleotides, was verified in lymphocytes from 56 patients under treatment for acute lymphoblastic leukemia (ALL) and from 33 healthy subjects. Patients were divided into 3 groups: remission induction (RI), remission maintenance (RM) and out-oftreatment (OT). It had been verified, in each group, the influence of the recurrence risk (high or low) on the enzymatic activity. The RM group was then subdivided by the amount of time they had been in remission maintenance (0-30, 31-60 and 61-84 weeks). Results demonstrated that the ATP hydrolysis from lymphocyte NTPDase1 is altered in the groups studied (F (3,84)=100.34; p<0.01) in relation to the controls, and that it is reduced in both RI and RM groups, while enhanced in OT group. The recurrence risk did not influence ATP hydrolysis in none of the groups. Furthermore, it has been found a significant variation at the NTPDase1 activity to ATP hydrolysis during RM phase (F (2,17)=11.22; p=0.01), being higher in the first 30 weeks, and subsequently reduced. ADP hydrolysis by NTPDase1 activity was also significantly reduced in both the RI and RM groups, but similar to the controls in OT (F (3,69)=59.05; p<0.01). It has not been verified any influence of the recurrence risk on ADP hydrolysis. Furhermore, NTPDase1 using ADP as substrate has shown to be constant during RM phase (F (2,13)=2.40; p=0.130). The alteration on ATP and ADP hydrolysis on lymphocytes of ALL patients is in agreement to the characteristics found in this pathology. Thus, our results corroborate the role of adenine nucleotides, ATP in special, in the immune system, as signaling molecules, and the importance of the NTPDase1 to maintain constant the extracellular levels of these nucleotides. However, more studies are necessary to better understand the role of adenine nucleotides and NTPDase1 in the immunodeficiency and citotoxicity observed in treated ALL patients.A atividade da enzima NTPDase1 (E.C. 3.6.1.5, CD39, apirase, ecto-ATPdifosfoidrolase), a qual hidrolisa os nucleotídeos ATP e ADP, foi verificada em linfócitos de 56 pacientes em tratamento para leucemia linfocítica aguda (LLA) e em 33 indivíduos controle. Os pacientes foram divididos em 3 grupos de acordo com a fase do tratamento: indução da remissão (IR), manutenção da remissão (MR) e fora-de-tratamento (FT), sendo que para cada grupo foi também verificada a influência do risco de recaída da doença (alto ou baixo) na atividade da enzima. O grupo MR foi ainda subdividido em três, de acordo com o período nesta fase: 0-30, 31-60 e 61-84 semanas em tratamento. Os resultados demonstraram que a hidrólise do ATP pela NTPDase1 está alterada nos 3 grupos estudados (F (3, 84)=100.34; p<0.01) em relação ao grupo controle, sendo que houve uma redução nas fases IR e MR, enquanto houve um aumento da hidrólise na fase FT. Não houve influência do prognóstico (grau de risco) na alteração da atividade da enzima em nenhum dos grupos estudados. Encontrou-se ainda uma variação na hidrólise do ATP pela enzima NTPDase1 durante a fase MR (F (2, 17)=11.22; p=001), sendo maior durante o período de 0-30 semanas de tratamento, e reduzida após. A atividade da NTPDase1 na hidrólise do ADP também se encontrou significativamente reduzida nas fases IR e MR, porém semelhante aos controles na fase FT (F (3, 69)=59.05; p<0.01). Também não foi verificada correlação entre a atividade da enzima, tendo ADP como substrato, e o prognóstico da LLA. Além disso, a atividade da NTPDase1 para o ADP se manteve constante durante a fase MR (F (2, 13)=2.40; p=0.130). A alteração na hidrólise do ATP e ADP em linfócitos de pacientes com LLA, como nossos resultados indicam, está de acordo com algumas das características encontradas durante o tratamento dessa patologia. Dessa forma, nossos resultados corroboram o papel dos nucleotídeos da adenina, em especial o ATP, no sistema imune como moléculas sinalizadoras, bem como a importância da enzima NTPDase1 para a manutenção dos níveis extracelulares desses nucleotídeos. Entretanto, mais estudos são necessários para melhor compreender o papel dos nucleotídeos da adenina e da NTPDase1 na imunodeficiência e citotoxicidade induzidos pelo tratamento da LLA.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaSchetinger, Maria Rosa ChitolinaBonan, Carla Denisehttp://lattes.cnpq.br/8058052532279136Graça, Dominguita Luhershttp://lattes.cnpq.br/7761878749916936Morsch, André Luis Bittencourt2006-11-102006-11-102006-08-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfMORSCH, André Luis Bittencourt. NTPDase1 activity in lymphocytes from patients under treatment for acute lymphoblastic leukemia (ALL): an alteration in the extracellular ATP and ADP hydrolysis. 2006. 85 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/11093porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-01-23T19:25:12Zoai:repositorio.ufsm.br:1/11093Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-01-23T19:25:12Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. NTPDase1 activity in lymphocytes from patients under treatment for acute lymphoblastic leukemia (ALL): an alteration in the extracellular ATP and ADP hydrolysis |
title |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
spellingShingle |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. Morsch, André Luis Bittencourt NTPDase1 Linfócitos ATP ADP Leucemia linfoblástica aguda Tratamento NTPDase1 Lymphocytes ATP ADP Acute lymphoblastic leukemia Treatment CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
title_full |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
title_fullStr |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
title_full_unstemmed |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
title_sort |
Atividade da enzima NTPDase1 em linfócitos de pacientes em tratamento para leucemia linfoblástica aguda (LLA): alteração na hidrólise do ATP e ADP extracelulares. |
author |
Morsch, André Luis Bittencourt |
author_facet |
Morsch, André Luis Bittencourt |
author_role |
author |
dc.contributor.none.fl_str_mv |
Schetinger, Maria Rosa Chitolina Bonan, Carla Denise http://lattes.cnpq.br/8058052532279136 Graça, Dominguita Luhers http://lattes.cnpq.br/7761878749916936 |
dc.contributor.author.fl_str_mv |
Morsch, André Luis Bittencourt |
dc.subject.por.fl_str_mv |
NTPDase1 Linfócitos ATP ADP Leucemia linfoblástica aguda Tratamento NTPDase1 Lymphocytes ATP ADP Acute lymphoblastic leukemia Treatment CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
NTPDase1 Linfócitos ATP ADP Leucemia linfoblástica aguda Tratamento NTPDase1 Lymphocytes ATP ADP Acute lymphoblastic leukemia Treatment CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The NTPDase1 (E.C. 3.6.1.5, apyrase, CD39, ecto-ATP-diphosphohydrolase) enzymatic activity, which is responsible for hydrolyzing ATP and ADP nucleotides, was verified in lymphocytes from 56 patients under treatment for acute lymphoblastic leukemia (ALL) and from 33 healthy subjects. Patients were divided into 3 groups: remission induction (RI), remission maintenance (RM) and out-oftreatment (OT). It had been verified, in each group, the influence of the recurrence risk (high or low) on the enzymatic activity. The RM group was then subdivided by the amount of time they had been in remission maintenance (0-30, 31-60 and 61-84 weeks). Results demonstrated that the ATP hydrolysis from lymphocyte NTPDase1 is altered in the groups studied (F (3,84)=100.34; p<0.01) in relation to the controls, and that it is reduced in both RI and RM groups, while enhanced in OT group. The recurrence risk did not influence ATP hydrolysis in none of the groups. Furthermore, it has been found a significant variation at the NTPDase1 activity to ATP hydrolysis during RM phase (F (2,17)=11.22; p=0.01), being higher in the first 30 weeks, and subsequently reduced. ADP hydrolysis by NTPDase1 activity was also significantly reduced in both the RI and RM groups, but similar to the controls in OT (F (3,69)=59.05; p<0.01). It has not been verified any influence of the recurrence risk on ADP hydrolysis. Furhermore, NTPDase1 using ADP as substrate has shown to be constant during RM phase (F (2,13)=2.40; p=0.130). The alteration on ATP and ADP hydrolysis on lymphocytes of ALL patients is in agreement to the characteristics found in this pathology. Thus, our results corroborate the role of adenine nucleotides, ATP in special, in the immune system, as signaling molecules, and the importance of the NTPDase1 to maintain constant the extracellular levels of these nucleotides. However, more studies are necessary to better understand the role of adenine nucleotides and NTPDase1 in the immunodeficiency and citotoxicity observed in treated ALL patients. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-11-10 2006-11-10 2006-08-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MORSCH, André Luis Bittencourt. NTPDase1 activity in lymphocytes from patients under treatment for acute lymphoblastic leukemia (ALL): an alteration in the extracellular ATP and ADP hydrolysis. 2006. 85 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006. http://repositorio.ufsm.br/handle/1/11093 |
identifier_str_mv |
MORSCH, André Luis Bittencourt. NTPDase1 activity in lymphocytes from patients under treatment for acute lymphoblastic leukemia (ALL): an alteration in the extracellular ATP and ADP hydrolysis. 2006. 85 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2006. |
url |
http://repositorio.ufsm.br/handle/1/11093 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922149464014848 |