Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS

Detalhes bibliográficos
Autor(a) principal: Uczay, Fernanda
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/00130000074sk
Texto Completo: http://repositorio.ufsm.br/handle/1/17156
Resumo: Soybeans and beans are considered oleaginous plants of great economic importance in Brasil. Maize is a cereal that also generates profitability for the economy, but its use, to a large extent, is as animal feed. In the last years, this crops suffered with infestations of the caterpillar Helicoperva Armigera, being the main problem the resistance of this pest to most pesticides. The indiscriminate use of pesticides and the lack of knowledge and use of good agricultural practices are the main causes of this, as well as undermining food security. Proof of this is the increased presence of pesticides in soil and agricultural crops. The aim of this work was to develop and validate a method based on QuEChERS extraction for the determination of residues of the avermectins: abamectin, emamectin benzoate, eprinomectin, ivermectin and doramectin in soybean, bean and maize samples by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Avemerctins were selected for the study because of the recent release of emamectin as a pesticide in the three matrices under study. Thus, a method for soybean, bean and maize was developed, where 5 g of sample and 10 mL of ultrapure water were used, as well as a 10 mL mixture of acetonitrile:isopropanol solvents in a ratio of 9:1 (v/v), with subsequent addition of partition salts. In the cleaning step five different sorbents were evaluated: C18 + PSA, Florisil®, silica, Supel™ QuE Z-Sep+ and EMR-Lipid, in their activated and non-activated form. With the clean-up step using EMR-Lipid, limits of detection of 1.2 μg kg-1 for abamectin, doramectin, emamectin benzoate and ivermectin, and of 2.4 μg kg-1 for eprinomectin were achieved. Accuracy and precision evaluated at the levels 4, 8, 20, 40 and 80 μg kg-1 presented satisfactory results. The method was successfully applied in real samples and is a good option for routine analysis
id UFSM_5337663331a8d5934df4f74928bdf3ce
oai_identifier_str oai:repositorio.ufsm.br:1/17156
network_acronym_str UFSM
network_name_str Manancial - Repositório Digital da UFSM
repository_id_str
spelling Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MSDetermination of avermectins residues in soybean, bean and maize using modified QuEChERS method and UHPLC-MS/MSLactonas macrocíclicasAvermectinasAlimentosPreparo de amostraCromatografia líquidaMacrocyclic lactonesAvermectinsFoodSample preparationLiquid chromatographyCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICASoybeans and beans are considered oleaginous plants of great economic importance in Brasil. Maize is a cereal that also generates profitability for the economy, but its use, to a large extent, is as animal feed. In the last years, this crops suffered with infestations of the caterpillar Helicoperva Armigera, being the main problem the resistance of this pest to most pesticides. The indiscriminate use of pesticides and the lack of knowledge and use of good agricultural practices are the main causes of this, as well as undermining food security. Proof of this is the increased presence of pesticides in soil and agricultural crops. The aim of this work was to develop and validate a method based on QuEChERS extraction for the determination of residues of the avermectins: abamectin, emamectin benzoate, eprinomectin, ivermectin and doramectin in soybean, bean and maize samples by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Avemerctins were selected for the study because of the recent release of emamectin as a pesticide in the three matrices under study. Thus, a method for soybean, bean and maize was developed, where 5 g of sample and 10 mL of ultrapure water were used, as well as a 10 mL mixture of acetonitrile:isopropanol solvents in a ratio of 9:1 (v/v), with subsequent addition of partition salts. In the cleaning step five different sorbents were evaluated: C18 + PSA, Florisil®, silica, Supel™ QuE Z-Sep+ and EMR-Lipid, in their activated and non-activated form. With the clean-up step using EMR-Lipid, limits of detection of 1.2 μg kg-1 for abamectin, doramectin, emamectin benzoate and ivermectin, and of 2.4 μg kg-1 for eprinomectin were achieved. Accuracy and precision evaluated at the levels 4, 8, 20, 40 and 80 μg kg-1 presented satisfactory results. The method was successfully applied in real samples and is a good option for routine analysisCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSoja e feijão são consideradas oleaginosas de grande importância econômica no Brasil. O milho é um cereal que também gera lucratividade para a economia, porém seu uso, em grande parte, é para alimentação animal. Nos últimos anos, essas culturas sofreram com infestações da largarta Helicoperva Armigera, sendo o principal problema associado a essa praga, a resistência a maioria dos agrotóxicos. O uso de forma indiscriminada de agrotóxicos e a falta de conhecimento e do emprego das boas práticas agrícolas são as principais causas desse fato, além de prejudicar a segurança alimentar. Prova disso, é o aumento da presença de agrotóxicos em solos e culturas agrícolas. Assim, o presente trabalho teve por objetivo desenvolver e validar um método baseado na extração pelo método QuEChERS para a determinação de resíduos de avermectinas: abamectina, benzoato de emamectina, eprinomectina, ivermectina e doramectina em amostras de soja, feijão e milho empregando a cromatografia líquida da ultra-alta eficiência acoplada à espectrometria de massas em série (UHPLC-MS/MS). As avemerctinas foram selecionadas para o estudo devido a recente liberação do emprego da emamectina como agrotóxico nas três matrizes em estudo. Com isso, desenvolveu-se um método para soja, feijão e milho, onde foi empregado 5 g de amostra e 10 mL de água, além de uma mistura de 10 mL dos solventes acetonitrila:isopropanol na proporção 9:1 (v/v), com posterior adição de sais de partição. Na etapa de limpeza avaliou-se cinco diferentes sorventes, sendo eles: C18+PSA, Florisil®, sílica, Supel™ QuE Z-Sep+ e EMR-Lipid, em sua forma ativada e não ativada. Com a etapa de limpeza usando EMR-Lipid, foram obtidos os limites de detecção de 1,2 μg kg-1 para abamectina, doramectina, benzoato de emamectina e ivermectina, e de 2,4 μg kg-1 para eprinomectina. Precisão e exatidão avaliadas nos níveis 4, 8, 20, 40 e 80 μg kg-1 apresentaram resultados satisfatórios. O método foi aplicado com sucesso em amostras reais e é uma boa opção para análises de rotina.Universidade Federal de Santa MariaBrasilQuímicaUFSMPrograma de Pós-Graduação em QuímicaCentro de Ciências Naturais e ExatasZanella, Renatohttp://lattes.cnpq.br/2541865299438479Prestes, Osmar Damianhttp://lattes.cnpq.br/9632234955509028Gonçalves, Fabio Ferreirahttp://lattes.cnpq.br/1090108357021843Adaime, Martha Bohrerhttp://lattes.cnpq.br/4385786922516848Uczay, Fernanda2019-06-26T15:42:08Z2019-06-26T15:42:08Z2019-02-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/17156ark:/26339/00130000074skporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-05-13T18:57:34Zoai:repositorio.ufsm.br:1/17156Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-05-13T18:57:34Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
Determination of avermectins residues in soybean, bean and maize using modified QuEChERS method and UHPLC-MS/MS
title Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
spellingShingle Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
Uczay, Fernanda
Lactonas macrocíclicas
Avermectinas
Alimentos
Preparo de amostra
Cromatografia líquida
Macrocyclic lactones
Avermectins
Food
Sample preparation
Liquid chromatography
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
title_full Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
title_fullStr Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
title_full_unstemmed Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
title_sort Determinação de resíduos de avermectinas em soja, feijão e milho empregando método QuEChERS modificado e UHPLC-MS/MS
author Uczay, Fernanda
author_facet Uczay, Fernanda
author_role author
dc.contributor.none.fl_str_mv Zanella, Renato
http://lattes.cnpq.br/2541865299438479
Prestes, Osmar Damian
http://lattes.cnpq.br/9632234955509028
Gonçalves, Fabio Ferreira
http://lattes.cnpq.br/1090108357021843
Adaime, Martha Bohrer
http://lattes.cnpq.br/4385786922516848
dc.contributor.author.fl_str_mv Uczay, Fernanda
dc.subject.por.fl_str_mv Lactonas macrocíclicas
Avermectinas
Alimentos
Preparo de amostra
Cromatografia líquida
Macrocyclic lactones
Avermectins
Food
Sample preparation
Liquid chromatography
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
topic Lactonas macrocíclicas
Avermectinas
Alimentos
Preparo de amostra
Cromatografia líquida
Macrocyclic lactones
Avermectins
Food
Sample preparation
Liquid chromatography
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description Soybeans and beans are considered oleaginous plants of great economic importance in Brasil. Maize is a cereal that also generates profitability for the economy, but its use, to a large extent, is as animal feed. In the last years, this crops suffered with infestations of the caterpillar Helicoperva Armigera, being the main problem the resistance of this pest to most pesticides. The indiscriminate use of pesticides and the lack of knowledge and use of good agricultural practices are the main causes of this, as well as undermining food security. Proof of this is the increased presence of pesticides in soil and agricultural crops. The aim of this work was to develop and validate a method based on QuEChERS extraction for the determination of residues of the avermectins: abamectin, emamectin benzoate, eprinomectin, ivermectin and doramectin in soybean, bean and maize samples by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Avemerctins were selected for the study because of the recent release of emamectin as a pesticide in the three matrices under study. Thus, a method for soybean, bean and maize was developed, where 5 g of sample and 10 mL of ultrapure water were used, as well as a 10 mL mixture of acetonitrile:isopropanol solvents in a ratio of 9:1 (v/v), with subsequent addition of partition salts. In the cleaning step five different sorbents were evaluated: C18 + PSA, Florisil®, silica, Supel™ QuE Z-Sep+ and EMR-Lipid, in their activated and non-activated form. With the clean-up step using EMR-Lipid, limits of detection of 1.2 μg kg-1 for abamectin, doramectin, emamectin benzoate and ivermectin, and of 2.4 μg kg-1 for eprinomectin were achieved. Accuracy and precision evaluated at the levels 4, 8, 20, 40 and 80 μg kg-1 presented satisfactory results. The method was successfully applied in real samples and is a good option for routine analysis
publishDate 2019
dc.date.none.fl_str_mv 2019-06-26T15:42:08Z
2019-06-26T15:42:08Z
2019-02-26
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/17156
dc.identifier.dark.fl_str_mv ark:/26339/00130000074sk
url http://repositorio.ufsm.br/handle/1/17156
identifier_str_mv ark:/26339/00130000074sk
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Química
UFSM
Programa de Pós-Graduação em Química
Centro de Ciências Naturais e Exatas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Química
UFSM
Programa de Pós-Graduação em Química
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
_version_ 1821325807741566976