Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4869 |
Resumo: | The knowledge of the level of association that occurs between diazotrophic bacteria and fungi arbusculares mycorrhizae (AMF) in grassland ecosystems may be important for the understanding of the changes caused by the addition of fertilizers containing phosphorus (P) and nitrogen (N), in the production and botanical composition of natural pastures. The objective of this study was to evaluate. (a) the occurrence of three genera of diazotrophic bacterial in the root system under fertilization with N and P, and determine the contribution of N via BNF, and (b) evaluate the behavior of the association between AMF and native grasses. Four most abundant grasses in natural grasslands of the Southern Campos in Rio Grande do Sul , Axonopus affinis, Paspalum notatum, Andropogon lateralis and Aristida laevis were grown in pots of 5 kg, in a greenhouse, two studies being conducted (A e B ). In A, two treatments were applied: 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP) and a control, being evaluated, the number of diazotrophic bacteria of the genera Azotobacter, Azospirillum and Herbaspirillum, and the contribution of BNF was determined by the technique of natural 15N abundance. In B, the treatments consisted of applying 50 mg kg-1 soil P (P), application of 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP), and a control, in both treatments mycorrhizal colonization was determined. For A, A. laevis demonstrate to be more dependent on biological N fixation than the other species. The grass P. notatum compared with other species demonstrated to be more efficient to absorb available soil N. The dry matter accumulation in shoots of the native species was higher with the application of NP. In B the mycorrhizal colonization was similar between the control, P and NP to the roots of A. lateralis and A. laevis, thus presenting a greater dependence on the mycorrhizal association. Differently, in A. affinis and P. notatum, the mycorrhizal colonization was lower when subjected to fertilization with P and NP, thus presenting a lower dependence. It is concluded for A that fertilization with N and P reduces diazotrophic colonization, increasing the production of dry matter and N content of the tissue. A. laevis showed the highest contribution of biological nitrogen fixation, since P. notatum showed higher N accumulation in soil. In relation to B, A. laevis and A. lateralis have a higher dependence on the mycorrhizal than A. affinis and P. notatum. |
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2014-10-232014-10-232014-02-10MARQUES, Anderson Cesar Ramos. Biological nitrogen fixation and mycorrhization in grasses of the southern fields. 2014. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/4869The knowledge of the level of association that occurs between diazotrophic bacteria and fungi arbusculares mycorrhizae (AMF) in grassland ecosystems may be important for the understanding of the changes caused by the addition of fertilizers containing phosphorus (P) and nitrogen (N), in the production and botanical composition of natural pastures. The objective of this study was to evaluate. (a) the occurrence of three genera of diazotrophic bacterial in the root system under fertilization with N and P, and determine the contribution of N via BNF, and (b) evaluate the behavior of the association between AMF and native grasses. Four most abundant grasses in natural grasslands of the Southern Campos in Rio Grande do Sul , Axonopus affinis, Paspalum notatum, Andropogon lateralis and Aristida laevis were grown in pots of 5 kg, in a greenhouse, two studies being conducted (A e B ). In A, two treatments were applied: 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP) and a control, being evaluated, the number of diazotrophic bacteria of the genera Azotobacter, Azospirillum and Herbaspirillum, and the contribution of BNF was determined by the technique of natural 15N abundance. In B, the treatments consisted of applying 50 mg kg-1 soil P (P), application of 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP), and a control, in both treatments mycorrhizal colonization was determined. For A, A. laevis demonstrate to be more dependent on biological N fixation than the other species. The grass P. notatum compared with other species demonstrated to be more efficient to absorb available soil N. The dry matter accumulation in shoots of the native species was higher with the application of NP. In B the mycorrhizal colonization was similar between the control, P and NP to the roots of A. lateralis and A. laevis, thus presenting a greater dependence on the mycorrhizal association. Differently, in A. affinis and P. notatum, the mycorrhizal colonization was lower when subjected to fertilization with P and NP, thus presenting a lower dependence. It is concluded for A that fertilization with N and P reduces diazotrophic colonization, increasing the production of dry matter and N content of the tissue. A. laevis showed the highest contribution of biological nitrogen fixation, since P. notatum showed higher N accumulation in soil. In relation to B, A. laevis and A. lateralis have a higher dependence on the mycorrhizal than A. affinis and P. notatum.O conhecimento do nível de associação que ocorre entre bactérias diazotróficas e fungos micorrízicos arbusculares (FMA) nos ecossistemas campestres, pode ser importante para a o entendimento das alterações provocadas pela adição de fertilizantes, contendo fósforo (P) e nitrogênio (N), na produção e na composição botânica das pastagens naturais. O objetivo do presente trabalho foi avaliar (a) a ocorrência de três gêneros de bactérias diazotróficas no sistema radicular sob fertilização com N e P, e determinar a contribuição de N via FBN, e (b) avaliar o comportamento da associação entre FMAs e gramíneas nativas. Foram utilizadas quatro gramíneas de maior abundância nas pastagens naturais dos Campos Sulinos no Rio Grande do Sul, Axonopus affinis, Paspalum notatum, Andropogon lateralis e Aristida laevis, cultivadas em casa de vegetação, sendo conduzidos dois estudos (A e B). Em A, foram aplicados dois tratamentos: 50 mg kg-1 de solo de P + 100 mg kg-1 de N solo (NP) e uma testemunha, sendo avaliados, o número de bactérias diazotróficas dos gêneros Azotobacter, Azospirillum e Herbaspirillum, e a contribuição da FBN através da técnica da abundância natural de 15N. Em B, os tratamentos consistiram na aplicação de 50 mg kg-1 de solo de P (P); aplicação de 50 mg kg-1 de solo de P + 100 mg kg-1 de N solo (NP), e uma testemunha, em ambos foi determinada a colonização micorrízica. A espécie A. laevis demonstrou ser mais dependente da fixação biológica de N que as demais espécies. A espécie P. notatum em comparação as demais espécies, demostrou ser mais hábil em absorver o N disponível no solo. A matéria seca acumulada da parte aérea das espécies nativas foi maior com a aplicação de NP. A colonização micorrízica foi semelhante entre a testemunha, P e NP para as raízes de A. lateralis e A. laevis, apresentando assim uma maior dependência da associação micorrízica. Diferentemente, nas espécies A. affinis e P. notatum, a colonização micorrízica foi menor quando submetidas a adubação com P e NP, apresentando assim uma menor dependência. Conclui-se assim, que a adubação com N e P reduz a colonização de bactérias diazotróficas, aumentando a produção de matéria seca e teor de N no tecido. A. laevis apresentou a maior contribuição da fixação biológica de nitrogênio, já P. notatum apresentou maior acúmulo de N do solo. As espécies A. laevis e A. lateralis apresentam maior dependência da micorrização que as espécies A. affinis e P. notatum.Empresa Brasileira de Pesquisa Agropecuáriaapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgrobiologiaUFSMBRCiências BiológicasRizosferaExsudatos radicularesBactérias associativasColonização micorrízicaDependência micorrízicaRhizosphereRoot exudatesAssociative bacteriaMycorrhizal colonizationMycorrhizal dependencyCNPQ::CIENCIAS BIOLOGICASFixação biológica de nitrogênio e micorrízação em gramíneas dos Campos SulinosBiological nitrogen fixation and mycorrhization in grasses of the southern fieldsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisQuadros, Fernando Luiz Ferreira dehttp://lattes.cnpq.br/9202266292366562Jacques, Rodrigo Josemar SeminotiVolk, Leandro Bochi da Silvahttp://lattes.cnpq.br/3828329485626314http://lattes.cnpq.br/5179294177118956Marques, Anderson Cesar Ramos20000000000640050030050030087f7a696-584d-4ee4-ad83-13fd04320556c82c097d-e90b-4eb8-9170-708b6a33502cda4dd5af-943f-4a59-a688-cbb4ef14d9b1b89028f7-9ef1-4745-a66e-3c6cef9c7f50info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALMARQUES, ANDERSON CESAR RAMOS.pdfapplication/pdf929247http://repositorio.ufsm.br/bitstream/1/4869/1/MARQUES%2c%20ANDERSON%20CESAR%20RAMOS.pdfa053e3f4f4630eb7e9568ec7cf50c19bMD51TEXTMARQUES, ANDERSON CESAR RAMOS.pdf.txtMARQUES, ANDERSON CESAR RAMOS.pdf.txtExtracted texttext/plain150729http://repositorio.ufsm.br/bitstream/1/4869/2/MARQUES%2c%20ANDERSON%20CESAR%20RAMOS.pdf.txte04fbb664769852a87b14ce591390dc3MD52THUMBNAILMARQUES, ANDERSON CESAR RAMOS.pdf.jpgMARQUES, ANDERSON CESAR RAMOS.pdf.jpgIM Thumbnailimage/jpeg4985http://repositorio.ufsm.br/bitstream/1/4869/3/MARQUES%2c%20ANDERSON%20CESAR%20RAMOS.pdf.jpg7943056c3591c5048326e2aa0cb8ad05MD531/48692023-06-06 15:55:16.774oai:repositorio.ufsm.br:1/4869Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-06-06T18:55:16Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
dc.title.alternative.eng.fl_str_mv |
Biological nitrogen fixation and mycorrhization in grasses of the southern fields |
title |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
spellingShingle |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos Marques, Anderson Cesar Ramos Rizosfera Exsudatos radiculares Bactérias associativas Colonização micorrízica Dependência micorrízica Rhizosphere Root exudates Associative bacteria Mycorrhizal colonization Mycorrhizal dependency CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
title_full |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
title_fullStr |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
title_full_unstemmed |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
title_sort |
Fixação biológica de nitrogênio e micorrízação em gramíneas dos Campos Sulinos |
author |
Marques, Anderson Cesar Ramos |
author_facet |
Marques, Anderson Cesar Ramos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Quadros, Fernando Luiz Ferreira de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9202266292366562 |
dc.contributor.referee1.fl_str_mv |
Jacques, Rodrigo Josemar Seminoti |
dc.contributor.referee2.fl_str_mv |
Volk, Leandro Bochi da Silva |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3828329485626314 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5179294177118956 |
dc.contributor.author.fl_str_mv |
Marques, Anderson Cesar Ramos |
contributor_str_mv |
Quadros, Fernando Luiz Ferreira de Jacques, Rodrigo Josemar Seminoti Volk, Leandro Bochi da Silva |
dc.subject.por.fl_str_mv |
Rizosfera Exsudatos radiculares Bactérias associativas Colonização micorrízica Dependência micorrízica |
topic |
Rizosfera Exsudatos radiculares Bactérias associativas Colonização micorrízica Dependência micorrízica Rhizosphere Root exudates Associative bacteria Mycorrhizal colonization Mycorrhizal dependency CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Rhizosphere Root exudates Associative bacteria Mycorrhizal colonization Mycorrhizal dependency |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
The knowledge of the level of association that occurs between diazotrophic bacteria and fungi arbusculares mycorrhizae (AMF) in grassland ecosystems may be important for the understanding of the changes caused by the addition of fertilizers containing phosphorus (P) and nitrogen (N), in the production and botanical composition of natural pastures. The objective of this study was to evaluate. (a) the occurrence of three genera of diazotrophic bacterial in the root system under fertilization with N and P, and determine the contribution of N via BNF, and (b) evaluate the behavior of the association between AMF and native grasses. Four most abundant grasses in natural grasslands of the Southern Campos in Rio Grande do Sul , Axonopus affinis, Paspalum notatum, Andropogon lateralis and Aristida laevis were grown in pots of 5 kg, in a greenhouse, two studies being conducted (A e B ). In A, two treatments were applied: 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP) and a control, being evaluated, the number of diazotrophic bacteria of the genera Azotobacter, Azospirillum and Herbaspirillum, and the contribution of BNF was determined by the technique of natural 15N abundance. In B, the treatments consisted of applying 50 mg kg-1 soil P (P), application of 50 mg kg-1 soil P and 100 mg kg-1 of soil N (NP), and a control, in both treatments mycorrhizal colonization was determined. For A, A. laevis demonstrate to be more dependent on biological N fixation than the other species. The grass P. notatum compared with other species demonstrated to be more efficient to absorb available soil N. The dry matter accumulation in shoots of the native species was higher with the application of NP. In B the mycorrhizal colonization was similar between the control, P and NP to the roots of A. lateralis and A. laevis, thus presenting a greater dependence on the mycorrhizal association. Differently, in A. affinis and P. notatum, the mycorrhizal colonization was lower when subjected to fertilization with P and NP, thus presenting a lower dependence. It is concluded for A that fertilization with N and P reduces diazotrophic colonization, increasing the production of dry matter and N content of the tissue. A. laevis showed the highest contribution of biological nitrogen fixation, since P. notatum showed higher N accumulation in soil. In relation to B, A. laevis and A. lateralis have a higher dependence on the mycorrhizal than A. affinis and P. notatum. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-10-23 |
dc.date.available.fl_str_mv |
2014-10-23 |
dc.date.issued.fl_str_mv |
2014-02-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARQUES, Anderson Cesar Ramos. Biological nitrogen fixation and mycorrhization in grasses of the southern fields. 2014. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4869 |
identifier_str_mv |
MARQUES, Anderson Cesar Ramos. Biological nitrogen fixation and mycorrhization in grasses of the southern fields. 2014. 73 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/4869 |
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por |
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Universidade Federal de Santa Maria |
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UFSM |
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BR |
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Ciências Biológicas |
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Universidade Federal de Santa Maria |
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