Patologia dos neoplamas malignos orais em cães
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/26756 |
Resumo: | This study aimed to characterize the cases of malignant neoplasms of the oral cavity of dogs from approximately 23 years of routine diagnosis at the Laboratory of Veterinary Pathology (LPV) of the Federal University of Santa Maria (UFSM). Through these data, two scientific articles were prepared that make up this dissertation. The first article is an overview of malignant neoplasms of the oral cavity, collecting data such as sex, race, age, clinical signs, neoplasm location, macroscopy and histology. During the study period, 205 dogs were affected by oral neoplasms, of which 101 were malignant. Of these, 47 were melanomas, 19 fibrosarcomas, 17 squamous cell carcinomas. Other less prevalent malignant neoplasms included undifferentiated sarcoma, mast cell tumor, hemangiosarcoma, osteosarcoma, transmissible venereal tumor, chondrosarcoma, and acinar cell carcinoma of the salivary gland. 51 cases were observed in females and 45 cases in males. Mixed-breed dogs totaled 33 cases and 64 dogs were defined-breed. The age range was from 1 to 19 years. The main clinical signs were swelling in the oral region, dysphagia, oral bleeding and drooling. The most affected sites were the gingiva, oral mucosa, mandible and mucocutaneous junction. Thus, through the two articles of this dissertation, it is possible to establish the frequency of these neoplasms in the studied period, as well as their clinical, macroscopic and histological, histochemical and immunohistochemical characteristics. The second article addresses a total of 47 melanomas in the oral cavity of dogs, in which clinical signs and anatomopathological aspects were characterized and the use of histochemical Toluidine Blue (TA) staining for the evidencing of melanin granules in comparison with the Fontana Masson (FM). In addition, the use of AT as a counterstain for the immunohistochemistry technique was evaluated. The clinical sign most frequently observed in dogs with oral melanoma was swelling in the oral region. The main site of involvement was gingiva (27.65%), followed by oral mucosa (21.27%) and mucocutaneous junction (12.76%), among others. Tumors were described as ulcerated nodules or masses, mostly between 0.5 and 2.0 cm in diameter. Histologically, the subtypes observed were epithelioid (44.6%), fusiform (27.6%), mixed epithelioid and fusiform (14.8%), ballooning (4.2%), small cells (4.25%), myxoid (2.12%) and round cells (2.12%). The histochemical techniques for quantification of melanin granules did not show differences between FM and AT. TA showed melanin granules as green to dark green dots or aggregates. Sections counterstained with AT and Harris' hematoxylin allowed a clear distinction between melanin (stained green) and immunostaining for Melan-A (stained brown). When the cells were MelanA-positive and had melanin in the cytoplasm, a third color pattern was shown (a mixture of yellow and green). |
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Patologia dos neoplamas malignos orais em cãesPathology of malignant oral neoplasm in dogsAzul de toluidinaCavidade oralMelan-ANeoplasmas oraisOral cavityOral neoplasmsToluidine blueCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThis study aimed to characterize the cases of malignant neoplasms of the oral cavity of dogs from approximately 23 years of routine diagnosis at the Laboratory of Veterinary Pathology (LPV) of the Federal University of Santa Maria (UFSM). Through these data, two scientific articles were prepared that make up this dissertation. The first article is an overview of malignant neoplasms of the oral cavity, collecting data such as sex, race, age, clinical signs, neoplasm location, macroscopy and histology. During the study period, 205 dogs were affected by oral neoplasms, of which 101 were malignant. Of these, 47 were melanomas, 19 fibrosarcomas, 17 squamous cell carcinomas. Other less prevalent malignant neoplasms included undifferentiated sarcoma, mast cell tumor, hemangiosarcoma, osteosarcoma, transmissible venereal tumor, chondrosarcoma, and acinar cell carcinoma of the salivary gland. 51 cases were observed in females and 45 cases in males. Mixed-breed dogs totaled 33 cases and 64 dogs were defined-breed. The age range was from 1 to 19 years. The main clinical signs were swelling in the oral region, dysphagia, oral bleeding and drooling. The most affected sites were the gingiva, oral mucosa, mandible and mucocutaneous junction. Thus, through the two articles of this dissertation, it is possible to establish the frequency of these neoplasms in the studied period, as well as their clinical, macroscopic and histological, histochemical and immunohistochemical characteristics. The second article addresses a total of 47 melanomas in the oral cavity of dogs, in which clinical signs and anatomopathological aspects were characterized and the use of histochemical Toluidine Blue (TA) staining for the evidencing of melanin granules in comparison with the Fontana Masson (FM). In addition, the use of AT as a counterstain for the immunohistochemistry technique was evaluated. The clinical sign most frequently observed in dogs with oral melanoma was swelling in the oral region. The main site of involvement was gingiva (27.65%), followed by oral mucosa (21.27%) and mucocutaneous junction (12.76%), among others. Tumors were described as ulcerated nodules or masses, mostly between 0.5 and 2.0 cm in diameter. Histologically, the subtypes observed were epithelioid (44.6%), fusiform (27.6%), mixed epithelioid and fusiform (14.8%), ballooning (4.2%), small cells (4.25%), myxoid (2.12%) and round cells (2.12%). The histochemical techniques for quantification of melanin granules did not show differences between FM and AT. TA showed melanin granules as green to dark green dots or aggregates. Sections counterstained with AT and Harris' hematoxylin allowed a clear distinction between melanin (stained green) and immunostaining for Melan-A (stained brown). When the cells were MelanA-positive and had melanin in the cytoplasm, a third color pattern was shown (a mixture of yellow and green).Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESEste estudo teve como objetivo caracterizar os casos de neoplasmas malignos da cavidade oral de cães provenientes de aproximadamente 23 anos da rotina diagnóstica do Laboratório de Patologia Veterinária (LPV) da Universidade Federal de Santa Maria (UFSM). Por meio desses dados, elaborou-se dois artigos científicos que compõem esta dissertação. O primeiro artigo, trata-se de um apanhado geral dos neoplasmas malignos de cavidade oral, coletando dados como sexo, raça, idade, sinais clínicos, localização do neoplasma, macroscopia e histologia. No período estudado, 205 cães foram acometidos por neoplasmas orais, dos quais 101 eram malignos. Destes, 47 eram melanomas, 19 fibrossarcomas, 17 carcinomas de células escamosas. Outros neoplasmas malignas menos prevalentes incluíram sarcoma indiferenciado, mastocitoma, hemangiossarcoma, osteossarcoma, tumor venéreo transmissível, condrossarcoma e carcinoma de células acinares da glândula salivar. Foram observados 51 casos em fêmeas e 45 casos em machos. Os cães sem raça definida totalizaram 33 casos e 64 cães tinham raça definida. A variação de idade foi de 1 a 19 anos. Os principais sinais clínicos foram aumento de volume na região oral, disfagia, sangramento oral e sialorreia. Os locais de maior acometimento foram a gengiva, mucosa oral, mandíbula e junção mucocutânea. Com isso, por meio dos dois artigos desta dissertação pode-se estabelecer a frequência dessas neoplasmas no período estudado, bem como suas características clínicas, macroscópicas e histológicas, histoquímicas e imuno-histoquímicas. O segundo artigo, aborda um total de 47 melanomas na cavidade oral de cães, no qual foram caracterizados os sinais clínicos e aspectos anatomopatológicos e foi avaliado o uso da coloração Azul de Toluidina (AT) histoquímica para a evidenciação de grânulos de melanina em comparação com o Fontana Masson (FM). Além disso, foi avaliado o uso de AT como contracoloração da técnica de imuno-histoquímica. O sinal clínico mais frequentemente observado em cães com melanoma oral foi o aumento de volume na região oral. O principal local de acometimento foi gengiva (27,65%), seguido de mucosa oral (21,27%) e junção mucocutânea (12,76%), entre outros. Os tumores foram descritos como nódulos ou massas, ulcerados, em sua maioria entre 0,5 e 2,0 cm de diâmetro. Histologicamente, os subtipos observados foram epitelioide (44,6%), fusiforme (27,6%), misto epitelioide e fusiforme (14,8%), balonoso (4,2%), células pequenas (4,25%), mixoide (2,12%) e células redondas (2,12%). As técnicas histoquímicas para quantificação dos grânulos de melanina não apresentaram diferenças entre FM e AT. O AT mostrou os grânulos de melanina como pontos ou agregados verdes a verde-escuros. Os cortes contracorados com AT e Hematoxilina de Harris permitiram uma distinção clara entre a melanina (corada em verde) e a imunomarcação para Melan-A (corada em marrom). Quando as células eram Melan-Apositivas e tinham melanina no citoplasma, um terceiro padrão de cor era mostrado (uma mistura de amarelo e verde).Universidade Federal de Santa MariaBrasilMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaCentro de Ciências RuraisKommers, Glaucia Denisehttp://lattes.cnpq.br/5818649889964582Fernandes, Cristina GevehrFlores, Mariana MartinsAguiar, Ariel de2022-11-04T16:47:42Z2022-11-04T16:47:42Z2022-10-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/26756porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-11-04T16:47:42Zoai:repositorio.ufsm.br:1/26756Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-11-04T16:47:42Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Patologia dos neoplamas malignos orais em cães Pathology of malignant oral neoplasm in dogs |
title |
Patologia dos neoplamas malignos orais em cães |
spellingShingle |
Patologia dos neoplamas malignos orais em cães Aguiar, Ariel de Azul de toluidina Cavidade oral Melan-A Neoplasmas orais Oral cavity Oral neoplasms Toluidine blue CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Patologia dos neoplamas malignos orais em cães |
title_full |
Patologia dos neoplamas malignos orais em cães |
title_fullStr |
Patologia dos neoplamas malignos orais em cães |
title_full_unstemmed |
Patologia dos neoplamas malignos orais em cães |
title_sort |
Patologia dos neoplamas malignos orais em cães |
author |
Aguiar, Ariel de |
author_facet |
Aguiar, Ariel de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Kommers, Glaucia Denise http://lattes.cnpq.br/5818649889964582 Fernandes, Cristina Gevehr Flores, Mariana Martins |
dc.contributor.author.fl_str_mv |
Aguiar, Ariel de |
dc.subject.por.fl_str_mv |
Azul de toluidina Cavidade oral Melan-A Neoplasmas orais Oral cavity Oral neoplasms Toluidine blue CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Azul de toluidina Cavidade oral Melan-A Neoplasmas orais Oral cavity Oral neoplasms Toluidine blue CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study aimed to characterize the cases of malignant neoplasms of the oral cavity of dogs from approximately 23 years of routine diagnosis at the Laboratory of Veterinary Pathology (LPV) of the Federal University of Santa Maria (UFSM). Through these data, two scientific articles were prepared that make up this dissertation. The first article is an overview of malignant neoplasms of the oral cavity, collecting data such as sex, race, age, clinical signs, neoplasm location, macroscopy and histology. During the study period, 205 dogs were affected by oral neoplasms, of which 101 were malignant. Of these, 47 were melanomas, 19 fibrosarcomas, 17 squamous cell carcinomas. Other less prevalent malignant neoplasms included undifferentiated sarcoma, mast cell tumor, hemangiosarcoma, osteosarcoma, transmissible venereal tumor, chondrosarcoma, and acinar cell carcinoma of the salivary gland. 51 cases were observed in females and 45 cases in males. Mixed-breed dogs totaled 33 cases and 64 dogs were defined-breed. The age range was from 1 to 19 years. The main clinical signs were swelling in the oral region, dysphagia, oral bleeding and drooling. The most affected sites were the gingiva, oral mucosa, mandible and mucocutaneous junction. Thus, through the two articles of this dissertation, it is possible to establish the frequency of these neoplasms in the studied period, as well as their clinical, macroscopic and histological, histochemical and immunohistochemical characteristics. The second article addresses a total of 47 melanomas in the oral cavity of dogs, in which clinical signs and anatomopathological aspects were characterized and the use of histochemical Toluidine Blue (TA) staining for the evidencing of melanin granules in comparison with the Fontana Masson (FM). In addition, the use of AT as a counterstain for the immunohistochemistry technique was evaluated. The clinical sign most frequently observed in dogs with oral melanoma was swelling in the oral region. The main site of involvement was gingiva (27.65%), followed by oral mucosa (21.27%) and mucocutaneous junction (12.76%), among others. Tumors were described as ulcerated nodules or masses, mostly between 0.5 and 2.0 cm in diameter. Histologically, the subtypes observed were epithelioid (44.6%), fusiform (27.6%), mixed epithelioid and fusiform (14.8%), ballooning (4.2%), small cells (4.25%), myxoid (2.12%) and round cells (2.12%). The histochemical techniques for quantification of melanin granules did not show differences between FM and AT. TA showed melanin granules as green to dark green dots or aggregates. Sections counterstained with AT and Harris' hematoxylin allowed a clear distinction between melanin (stained green) and immunostaining for Melan-A (stained brown). When the cells were MelanA-positive and had melanin in the cytoplasm, a third color pattern was shown (a mixture of yellow and green). |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-04T16:47:42Z 2022-11-04T16:47:42Z 2022-10-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26756 |
url |
http://repositorio.ufsm.br/handle/1/26756 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922058831396864 |