Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8701 |
Resumo: | Pinus elliottii is an important forestal culture, whose seeds, in terms of health quality, display high vulnerability to fungal pathogenic agents, especially to the association of especially by association of Fusarium spp. which is a responsible agent for seedlings losses in the nursery. This study aims to determine the appropriate method to assess the pathogenicity of Fusarium sp. associated with seeds of P. elliottii, and characterize the fungus, morphological- and molecularly, on its species level, as well as to verify the efficiency of its in vitro and in vivo biological control. Pathogenicity to different treatments were applied: T1 - Contact + Fusarium sp.; T2 - Contact + film coating + Fusarium sp.; T3 - film coating + Fusarium sp.; T4 film coating; T5 - Contact PDA and T6 - No treatment. Morphological characterization was performed using a specific identification key for the genus Fusarium, and the molecular identification, we sequenced three genomic regions of isolate, which are: ITS, elongation factor 1-α and beta-tubulin. In tests of biological control used is Trichoderma spp. and Bacillus subtilis in the commercial forms Agrotrich Plus® and Rizolyptus®, respectively. The control tests were performed in vitro by the method of paired comparison of cultures (pathogen x antagonist) and in vivo tests were carried out in nursery conditions. Fusarium s species that was identified on the seeds was characterized as Fusarium sambucinum, which is pathogenic to P. elliottii, when the seeds are inoculated via contact to the fungal culture during 48 hours. Microbilization of seeds with Bacillus subtilis and Trichoderma spp. provided improvement on the germination potential of them, and, in the case of Agrotrich Plus®, reduced the incidence of rot agent fungi, such as Penicillium sp. and Aspergillus sp.. The antagonistic agents were efficient on the in vitro control of F. sambucinum. Rizolyptus® stood out against the pathogen on the in vivo biocontrol test; it reduced seedlings losses and increased values of their length, as well as, of their green and dry masses. |
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2017-02-142017-02-142012-07-16MACIEL, Caciara Gonzatto. Fusarium sambucinum associated to seeds of Pinus elliottii: pathogenicity, morphology, molecular phylogeny and control. 2012. 94 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/8701Pinus elliottii is an important forestal culture, whose seeds, in terms of health quality, display high vulnerability to fungal pathogenic agents, especially to the association of especially by association of Fusarium spp. which is a responsible agent for seedlings losses in the nursery. This study aims to determine the appropriate method to assess the pathogenicity of Fusarium sp. associated with seeds of P. elliottii, and characterize the fungus, morphological- and molecularly, on its species level, as well as to verify the efficiency of its in vitro and in vivo biological control. Pathogenicity to different treatments were applied: T1 - Contact + Fusarium sp.; T2 - Contact + film coating + Fusarium sp.; T3 - film coating + Fusarium sp.; T4 film coating; T5 - Contact PDA and T6 - No treatment. Morphological characterization was performed using a specific identification key for the genus Fusarium, and the molecular identification, we sequenced three genomic regions of isolate, which are: ITS, elongation factor 1-α and beta-tubulin. In tests of biological control used is Trichoderma spp. and Bacillus subtilis in the commercial forms Agrotrich Plus® and Rizolyptus®, respectively. The control tests were performed in vitro by the method of paired comparison of cultures (pathogen x antagonist) and in vivo tests were carried out in nursery conditions. Fusarium s species that was identified on the seeds was characterized as Fusarium sambucinum, which is pathogenic to P. elliottii, when the seeds are inoculated via contact to the fungal culture during 48 hours. Microbilization of seeds with Bacillus subtilis and Trichoderma spp. provided improvement on the germination potential of them, and, in the case of Agrotrich Plus®, reduced the incidence of rot agent fungi, such as Penicillium sp. and Aspergillus sp.. The antagonistic agents were efficient on the in vitro control of F. sambucinum. Rizolyptus® stood out against the pathogen on the in vivo biocontrol test; it reduced seedlings losses and increased values of their length, as well as, of their green and dry masses.Pinus elliottii é uma espécie de importância no setor florestal e que apresenta vulnerabilidade na qualidade sanitária de suas sementes, especialmente pela associação de Fusarium spp., responsável por perdas de plântulas no viveiro. O presente trabalho tem como objetivo determinar o método adequado para avaliar a patogenicidade de Fusarium spp. associado a sementes de P. elliottii e caracterizar morfologica e molecularmente o patógeno em nível de espécie, bem como verificar a eficiência do controle biológico in vitro e in vivo do fungo. Para patogenicidade foram aplicados diferentes tratamentos: T1- Contato + Fusarium sp.; T2 - Peliculização + Contato + Fusarium sp.; T3 - Peliculização + Fusarium sp.; T4 Peliculização; T5 - Contato com meio BDA e T6 - Sem tratamento. A caracterização morfológica foi realizada, utilizando uma chave de identificação específica para o gênero Fusarium, e para a identificação molecular, foram sequenciadas três regiões genômicas do isolado, sendo elas: ITS, fator de elongação 1- α e beta-tubulina. Nos testes de controle biológico utilizou-se Trichoderma spp. e Bacillus subtilis, nas formas comerciais Agrotrich Plus® e Rizolyptus®, respectivamente. Os testes de controle in vitro foram realizados pelo método de confronto pareado de culturas (antagonista x patógeno) e os testes in vivo, foram desenvolvidos em condições de viveiro. A espécie de Fusarium identificada nas sementes foi caracterizada como Fusarium sambucinum, patogênico a P. elliottii, quando inoculado via contato com a cultura fúngica por 48 horas. A microbiolização das sementes com Rizolyptus® e Agrotrich Plus® promoveu incremento no potencial germinativo e, no caso do Agrotrich Plus®, reduziu a incidência de fungos apodrecedores como Penicillium sp. e Aspergillus sp. Os antagonistas foram eficientes no controle in vitro de F. sambucinum e, no teste de biocontrole in vivo, o produto Rizolyptus® destacou-se, reduzindo as perdas de plântulas causadas pelo patógeno, assim como, potencializando as variáveis de comprimento de plântula, massa verde e massa seca.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Engenharia FlorestalUFSMBRRecursos Florestais e Engenharia FlorestalMicrobiolizaçãoBacillus subtilisTrichoderma sppMicrobiolizationCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALFusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controleFusarium sambucinum associated to seeds of Pinus elliottii: pathogenicity, morphology, molecular phylogeny and controlinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMuniz, Marlove Fatima Briãohttp://lattes.cnpq.br/3148312031889388Oliveira, Luciana Magda dehttp://lattes.cnpq.br/6241707717454276Poletto, Igorhttp://lattes.cnpq.br/5851156257793439http://lattes.cnpq.br/1178735744894291Maciel, Caciara Gonzatto500200000003400500500300500e8de5014-a63f-4739-8254-6b23c08bcd595cc137e4-e44d-45c1-b16d-105e9a1cdf22be4adee8-71fa-4328-ab5c-5fa0d1cf04bc5dfc8864-aaf7-44f5-b487-910c74a8a17ainfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALMACIEL, CACIARA GONZATTO.pdfapplication/pdf3652516http://repositorio.ufsm.br/bitstream/1/8701/1/MACIEL%2c%20CACIARA%20GONZATTO.pdf402614f910af5be4db2d06f9e2782f0bMD51TEXTMACIEL, CACIARA GONZATTO.pdf.txtMACIEL, CACIARA GONZATTO.pdf.txtExtracted texttext/plain178514http://repositorio.ufsm.br/bitstream/1/8701/2/MACIEL%2c%20CACIARA%20GONZATTO.pdf.txtee955720b93972c0203cecffaeee8800MD52THUMBNAILMACIEL, CACIARA GONZATTO.pdf.jpgMACIEL, CACIARA GONZATTO.pdf.jpgIM Thumbnailimage/jpeg5009http://repositorio.ufsm.br/bitstream/1/8701/3/MACIEL%2c%20CACIARA%20GONZATTO.pdf.jpg3e4cd3f78c9cb6d856a978e8dc6a686dMD531/87012022-07-12 10:46:50.905oai:repositorio.ufsm.br:1/8701Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-07-12T13:46:50Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
dc.title.alternative.eng.fl_str_mv |
Fusarium sambucinum associated to seeds of Pinus elliottii: pathogenicity, morphology, molecular phylogeny and control |
title |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
spellingShingle |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle Maciel, Caciara Gonzatto Microbiolização Bacillus subtilis Trichoderma spp Microbiolization CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
title_full |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
title_fullStr |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
title_full_unstemmed |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
title_sort |
Fusarium sambucinum associado a sementes de Pinus elliottii: patogenicidade, morfologia, filogenia molecular e controle |
author |
Maciel, Caciara Gonzatto |
author_facet |
Maciel, Caciara Gonzatto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Muniz, Marlove Fatima Brião |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3148312031889388 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Luciana Magda de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6241707717454276 |
dc.contributor.referee2.fl_str_mv |
Poletto, Igor |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5851156257793439 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1178735744894291 |
dc.contributor.author.fl_str_mv |
Maciel, Caciara Gonzatto |
contributor_str_mv |
Muniz, Marlove Fatima Brião Oliveira, Luciana Magda de Poletto, Igor |
dc.subject.por.fl_str_mv |
Microbiolização Bacillus subtilis Trichoderma spp |
topic |
Microbiolização Bacillus subtilis Trichoderma spp Microbiolization CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
dc.subject.eng.fl_str_mv |
Microbiolization |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
Pinus elliottii is an important forestal culture, whose seeds, in terms of health quality, display high vulnerability to fungal pathogenic agents, especially to the association of especially by association of Fusarium spp. which is a responsible agent for seedlings losses in the nursery. This study aims to determine the appropriate method to assess the pathogenicity of Fusarium sp. associated with seeds of P. elliottii, and characterize the fungus, morphological- and molecularly, on its species level, as well as to verify the efficiency of its in vitro and in vivo biological control. Pathogenicity to different treatments were applied: T1 - Contact + Fusarium sp.; T2 - Contact + film coating + Fusarium sp.; T3 - film coating + Fusarium sp.; T4 film coating; T5 - Contact PDA and T6 - No treatment. Morphological characterization was performed using a specific identification key for the genus Fusarium, and the molecular identification, we sequenced three genomic regions of isolate, which are: ITS, elongation factor 1-α and beta-tubulin. In tests of biological control used is Trichoderma spp. and Bacillus subtilis in the commercial forms Agrotrich Plus® and Rizolyptus®, respectively. The control tests were performed in vitro by the method of paired comparison of cultures (pathogen x antagonist) and in vivo tests were carried out in nursery conditions. Fusarium s species that was identified on the seeds was characterized as Fusarium sambucinum, which is pathogenic to P. elliottii, when the seeds are inoculated via contact to the fungal culture during 48 hours. Microbilization of seeds with Bacillus subtilis and Trichoderma spp. provided improvement on the germination potential of them, and, in the case of Agrotrich Plus®, reduced the incidence of rot agent fungi, such as Penicillium sp. and Aspergillus sp.. The antagonistic agents were efficient on the in vitro control of F. sambucinum. Rizolyptus® stood out against the pathogen on the in vivo biocontrol test; it reduced seedlings losses and increased values of their length, as well as, of their green and dry masses. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-16 |
dc.date.accessioned.fl_str_mv |
2017-02-14 |
dc.date.available.fl_str_mv |
2017-02-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACIEL, Caciara Gonzatto. Fusarium sambucinum associated to seeds of Pinus elliottii: pathogenicity, morphology, molecular phylogeny and control. 2012. 94 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/8701 |
identifier_str_mv |
MACIEL, Caciara Gonzatto. Fusarium sambucinum associated to seeds of Pinus elliottii: pathogenicity, morphology, molecular phylogeny and control. 2012. 94 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
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http://repositorio.ufsm.br/handle/1/8701 |
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por |
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UFSM |
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BR |
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Recursos Florestais e Engenharia Florestal |
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Universidade Federal de Santa Maria |
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