Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos

Detalhes bibliográficos
Autor(a) principal: Quadros, Vanessa Andreatta de
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/11247
Resumo: The zebrafish (Danio rerio) is a small teleost widely used in several scientific researches. Among the characteristics that make zebrafish an attractive model organism when compared to rodents, we highlight the easy maintenance, manipulation, and the feasibility for the evaluation of neurochemical aspects that influence animal behavior. The zebrafish genes have been already sequenced and present a high degree of homology in relation to their human counterparts, which allows the use of this species in translational studies of stress and defensive behavior. In fish, exposure to alarm substance from conspecifics is an experimental model that induces fear, naturally mimicking the chemical communication that occur during the attack of predators. In the literature, the behavioral analysis of two zebrafish strains (wild type WT and leopard leo ) showed that leo presents an exacerbation of anxiety-like behaviors. This aspect could be an important characteristic for assessing the defensive behaviors triggered by alarm substance in distinct experimental approaches. Thus, the goal of this work was to evaluate the behavioral profile of WT and leo acutely exposed to alarm substance from conspecifics (5 min) at three contexts: during the alarm substance exposure period (Experiment 1); after exposure, in habituation to novelty (Experiment 2); or after exposure, in the light-dark test (Experiment 3). Furthermore, we verified the influence of alarm substance on pigment response. During the alarm substance exposure (Experiment 1), leo increased the vertical drifts, as well as the number and duration of erratic movements, while WT increased the number of erratic movements and the latency to enter the upper area of the apparatus. Both strains did not present significant changes on pigment response. In the novel tank test (Experiment 2), WT presented increased fear responses. On the other hand, leo increased the vertical exploration, indicating differences on habituation to novelty. In the light-dark test (Experiment 3), the alarm substance promoted a significant increase in the number of erratic movements, but elicited different responses in scototaxis, in the latency to enter the dark compartment, and in the number of risk assessment episodes. Principal component analyses suggested that burst swimming, anxiety-like behaviors, and locomotion/exploration were the main factors that contributed to the results obtained in the Experiments 1, 2, and 3, respectively. Altogether, the behavioral responses of alarm substance from conspecifics in zebrafish are strain- and context-dependent.
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spelling Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextosEvaluation of the behavioral profile of two zebrafish strains exposed to alarm substance at different contextsMedoAnsiedadeComportamentos defensivosPeixe zebraFearAnxietyDefensive behaviorsZebrafishCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAThe zebrafish (Danio rerio) is a small teleost widely used in several scientific researches. Among the characteristics that make zebrafish an attractive model organism when compared to rodents, we highlight the easy maintenance, manipulation, and the feasibility for the evaluation of neurochemical aspects that influence animal behavior. The zebrafish genes have been already sequenced and present a high degree of homology in relation to their human counterparts, which allows the use of this species in translational studies of stress and defensive behavior. In fish, exposure to alarm substance from conspecifics is an experimental model that induces fear, naturally mimicking the chemical communication that occur during the attack of predators. In the literature, the behavioral analysis of two zebrafish strains (wild type WT and leopard leo ) showed that leo presents an exacerbation of anxiety-like behaviors. This aspect could be an important characteristic for assessing the defensive behaviors triggered by alarm substance in distinct experimental approaches. Thus, the goal of this work was to evaluate the behavioral profile of WT and leo acutely exposed to alarm substance from conspecifics (5 min) at three contexts: during the alarm substance exposure period (Experiment 1); after exposure, in habituation to novelty (Experiment 2); or after exposure, in the light-dark test (Experiment 3). Furthermore, we verified the influence of alarm substance on pigment response. During the alarm substance exposure (Experiment 1), leo increased the vertical drifts, as well as the number and duration of erratic movements, while WT increased the number of erratic movements and the latency to enter the upper area of the apparatus. Both strains did not present significant changes on pigment response. In the novel tank test (Experiment 2), WT presented increased fear responses. On the other hand, leo increased the vertical exploration, indicating differences on habituation to novelty. In the light-dark test (Experiment 3), the alarm substance promoted a significant increase in the number of erratic movements, but elicited different responses in scototaxis, in the latency to enter the dark compartment, and in the number of risk assessment episodes. Principal component analyses suggested that burst swimming, anxiety-like behaviors, and locomotion/exploration were the main factors that contributed to the results obtained in the Experiments 1, 2, and 3, respectively. Altogether, the behavioral responses of alarm substance from conspecifics in zebrafish are strain- and context-dependent.Conselho Nacional de Desenvolvimento Científico e TecnológicoO peixe zebra (Danio rerio) é um pequeno teleósteo amplamente utilizado em pesquisas científicas nas diferentes áreas do conhecimento. Dentre as características que tornam o peixe zebra um atrativo organismo modelo quando comparado com roedores podemos citar a facilidade de manutenção, a manipulação e a viabilidade para a avaliação de aspectos neuroquímicos que influenciam o comportamento animal. O genoma do peixe zebra já foi totalmente sequenciado e sua alta homologia com genes humanos possibilita a utilização da espécie em estudos translacionais de estresse e comportamento defensivo. Em peixes, a exposição à substância de alarme de co-específicos é um modelo experimental capaz de induzir medo, mimetizando de modo natural a comunicação química que ocorre em situações de ataque de predadores. Na literatura, a análise comportamental de duas linhagens de peixe zebra (selvagem WT e leopardo leo ) demonstrou que leo apresenta uma exacerbação do comportamento tipo ansiedade, o que poderia ser um importante aspecto para a investigação dos comportamentos defensivos desencadeados pela substância de alarme em diferentes situações experimentais. Assim, o objetivo deste trabalho foi avaliar o padrão comportamental das linhagens de peixe zebra WT e leo expostas agudamente à substância de alarme (5 min) em três contextos: durante o período de exposição (Experimento 1); após a exposição, em respostas de habituação à novidade (Experimento 2); ou após a exposição, na preferência claro-escuro (Experimento 3). Além disso, verificamos a influência da substância de alarme sobre a resposta pigmentar de ambas as linhagens. Durante a exposição à substância de alarme (Experimento 1), a linhagem leo aumentou o nado vertical, bem como o número e a duração de movimentos erráticos, enquanto que WT aumentou o número de movimentos erráticos e a latência para entrada na área superior do aparato. Ambas as linhagens não apresentaram variação no padrão de pigmentação. No teste do tanque novo (Experimento 2), a linhagem WT apresentou mais respostas relacionadas ao medo. Por outro lado, leo apresentou um aumento das transições e do tempo gasto no topo, indicando diferenças na habituação. A substância de alarme aumentou o número de movimentos erráticos no teste claro-escuro (Experimento 3), mas desencadeou diferentes respostas na escototaxia, na latência para entrada no compartimento escuro e nos episódios de avaliação de risco. A análise principal de componentes sugeriu que o nado acelerado, comportamentos tipo ansiedade e locomoção/exploração foram os fatores que mais contribuíram para os resultados obtidos nos Experimentos 1, 2 e 3, respectivamente. Em suma, a substância de alarme apresenta uma resposta dependente de linhagem e contexto em peixe zebra.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaRosemberg, Denis Broockhttp://lattes.cnpq.br/7713953979203056Oliveira, Sara Marchesan dehttp://lattes.cnpq.br/6574555059806902Piato, Angelo Luis Stapassolihttp://lattes.cnpq.br/0837379287129794Quadros, Vanessa Andreatta de2016-09-212016-09-212016-07-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfQUADROS, Vanessa Andreatta de. Evaluation of the behavioral profile of two zebrafish strains exposed to alarm substance at different contexts. 2016. 50 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/11247porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-06T18:24:38Zoai:repositorio.ufsm.br:1/11247Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-06T18:24:38Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
Evaluation of the behavioral profile of two zebrafish strains exposed to alarm substance at different contexts
title Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
spellingShingle Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
Quadros, Vanessa Andreatta de
Medo
Ansiedade
Comportamentos defensivos
Peixe zebra
Fear
Anxiety
Defensive behaviors
Zebrafish
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
title_full Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
title_fullStr Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
title_full_unstemmed Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
title_sort Avaliação do padrão comportamental de duas linhagens de peixe zebra expostas à substância de alarme em diferentes contextos
author Quadros, Vanessa Andreatta de
author_facet Quadros, Vanessa Andreatta de
author_role author
dc.contributor.none.fl_str_mv Rosemberg, Denis Broock
http://lattes.cnpq.br/7713953979203056
Oliveira, Sara Marchesan de
http://lattes.cnpq.br/6574555059806902
Piato, Angelo Luis Stapassoli
http://lattes.cnpq.br/0837379287129794
dc.contributor.author.fl_str_mv Quadros, Vanessa Andreatta de
dc.subject.por.fl_str_mv Medo
Ansiedade
Comportamentos defensivos
Peixe zebra
Fear
Anxiety
Defensive behaviors
Zebrafish
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Medo
Ansiedade
Comportamentos defensivos
Peixe zebra
Fear
Anxiety
Defensive behaviors
Zebrafish
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description The zebrafish (Danio rerio) is a small teleost widely used in several scientific researches. Among the characteristics that make zebrafish an attractive model organism when compared to rodents, we highlight the easy maintenance, manipulation, and the feasibility for the evaluation of neurochemical aspects that influence animal behavior. The zebrafish genes have been already sequenced and present a high degree of homology in relation to their human counterparts, which allows the use of this species in translational studies of stress and defensive behavior. In fish, exposure to alarm substance from conspecifics is an experimental model that induces fear, naturally mimicking the chemical communication that occur during the attack of predators. In the literature, the behavioral analysis of two zebrafish strains (wild type WT and leopard leo ) showed that leo presents an exacerbation of anxiety-like behaviors. This aspect could be an important characteristic for assessing the defensive behaviors triggered by alarm substance in distinct experimental approaches. Thus, the goal of this work was to evaluate the behavioral profile of WT and leo acutely exposed to alarm substance from conspecifics (5 min) at three contexts: during the alarm substance exposure period (Experiment 1); after exposure, in habituation to novelty (Experiment 2); or after exposure, in the light-dark test (Experiment 3). Furthermore, we verified the influence of alarm substance on pigment response. During the alarm substance exposure (Experiment 1), leo increased the vertical drifts, as well as the number and duration of erratic movements, while WT increased the number of erratic movements and the latency to enter the upper area of the apparatus. Both strains did not present significant changes on pigment response. In the novel tank test (Experiment 2), WT presented increased fear responses. On the other hand, leo increased the vertical exploration, indicating differences on habituation to novelty. In the light-dark test (Experiment 3), the alarm substance promoted a significant increase in the number of erratic movements, but elicited different responses in scototaxis, in the latency to enter the dark compartment, and in the number of risk assessment episodes. Principal component analyses suggested that burst swimming, anxiety-like behaviors, and locomotion/exploration were the main factors that contributed to the results obtained in the Experiments 1, 2, and 3, respectively. Altogether, the behavioral responses of alarm substance from conspecifics in zebrafish are strain- and context-dependent.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-21
2016-09-21
2016-07-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv QUADROS, Vanessa Andreatta de. Evaluation of the behavioral profile of two zebrafish strains exposed to alarm substance at different contexts. 2016. 50 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2016.
http://repositorio.ufsm.br/handle/1/11247
identifier_str_mv QUADROS, Vanessa Andreatta de. Evaluation of the behavioral profile of two zebrafish strains exposed to alarm substance at different contexts. 2016. 50 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2016.
url http://repositorio.ufsm.br/handle/1/11247
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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