Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi

Detalhes bibliográficos
Autor(a) principal: Tonin, Alexandre Alberto
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/0013000014fk0
Texto Completo: http://repositorio.ufsm.br/handle/1/8954
Resumo: The aim of this study was to evaluate the utilization of a standard treatment with diminazene aceturate against the infection caused by T. evansi, associated to sodium selenite and vitamin E. In vitro tests showed trypanocidal effect related to the treatment with diminazene aceturate and sodium selenite, but vitamin E had no harmful effect on the trypanosomes. In vivo experiments utilized a total of 72 adult outbreed females rats, separated into 9 groups (A, B, C, D, E, F, G, H and I), 8 animals each. Group A was the uninfected group; groups B to I were infected with 0.2 mL of blood containing 106 trypanosomes. Parasitemia was estimated daily by microscopic examination of blood smears. Group B served as positive control; group C was treated with diminazene aceturate; group D with sodium selenite; group E with vitamin E; group F received an association of diminazene aceturate and sodium selenite; group G received an association of diminazene aceturate and vitamin E; group H received an association of diminazene aceturate, sodium selenite and vitamin E, and group I received an association of sodium selenite and vitamin E. Diminazene aceturate was administrated in a single dose on the 3rd day post infection (PI). Sodium selenite and vitamin E were administered at the 3rd and 23rd day PI. In vivo tests showed increase of longevity in groups treated with diminazene aceturate associated with sodium selenite (groups F and H). No difference was found between groups C and E, thus the vitamin E did not increase the efficacy of treatment against T. evansi when associated to diminazene aceturate. The curative efficacy of treatments was 37.5, 87.7, 37.7 and 75% to the groups C, F, G and H, respectively. Other treatments showed no efficacy. The sodium selenite when combined with chemotherapy may represent an alternative in the treatment of trypanosomosis.
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spelling Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansiDiminazene aceturate associated to sodium selenite and vitamin E: tests in vitro and in rats experimentally infected with Trypanosoma evansiTrypanosoma evansiAceturato de diminazenoSelenito de sódioVitamina ERoedoresTrypanosoma evansiDiminazene aceturateSodium seleniteVitamin ERodentsCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAThe aim of this study was to evaluate the utilization of a standard treatment with diminazene aceturate against the infection caused by T. evansi, associated to sodium selenite and vitamin E. In vitro tests showed trypanocidal effect related to the treatment with diminazene aceturate and sodium selenite, but vitamin E had no harmful effect on the trypanosomes. In vivo experiments utilized a total of 72 adult outbreed females rats, separated into 9 groups (A, B, C, D, E, F, G, H and I), 8 animals each. Group A was the uninfected group; groups B to I were infected with 0.2 mL of blood containing 106 trypanosomes. Parasitemia was estimated daily by microscopic examination of blood smears. Group B served as positive control; group C was treated with diminazene aceturate; group D with sodium selenite; group E with vitamin E; group F received an association of diminazene aceturate and sodium selenite; group G received an association of diminazene aceturate and vitamin E; group H received an association of diminazene aceturate, sodium selenite and vitamin E, and group I received an association of sodium selenite and vitamin E. Diminazene aceturate was administrated in a single dose on the 3rd day post infection (PI). Sodium selenite and vitamin E were administered at the 3rd and 23rd day PI. In vivo tests showed increase of longevity in groups treated with diminazene aceturate associated with sodium selenite (groups F and H). No difference was found between groups C and E, thus the vitamin E did not increase the efficacy of treatment against T. evansi when associated to diminazene aceturate. The curative efficacy of treatments was 37.5, 87.7, 37.7 and 75% to the groups C, F, G and H, respectively. Other treatments showed no efficacy. The sodium selenite when combined with chemotherapy may represent an alternative in the treatment of trypanosomosis.Conselho Nacional de Desenvolvimento Científico e TecnológicoO objetivo deste estudo foi avaliar a utilização de um tratamento padrão contra a infecção causada pelo T. evansi, baseado na utilização do aceturato de diminazeno associado ao selenito de sódio e a vitamina E. Os testes in vitro mostraram um efeito tripanocida relacionados ao tratamento com aceturato de diminazeno e selenito de sódio; contudo a vitamina E não gerou nenhum efeito nocivo sobre o tripanossomas. Experimentos in vivo utilizaram um total de 72 fêmeas adultas de ratos, separados em 9 grupos (A, B, C, D, E, F, G, H e I), com 8 animais cada grupo. O grupo A serviu como grupo não infectado; grupos de B a I foram infectados com 0,2 mL de sangue contendo 106 tripanossomas. A parasitemia foi estimada diariamente por exame microscópico de esfregaços sanguíneo. O grupo B serviu como controle positivo; grupo C, tratado com aceturato de diminazeno; grupo D, com selenito de sódio; grupo E, com vitamina E; grupo F, recebeu uma associação de aceturato de diminazeno e selenito de sódio; grupo G, associação de aceturato de diminazeno e vitamina E; grupo H, associação de aceturato de diminazeno, selenito de sódio e vitamina E; e por fim o grupo I o qual recebeu uma associação de selenito de sódio e vitamina E. O aceturato de diminazeno foi administrado em dose única no 3º dia pós-infecção (PI). Selenito de sódio e vitamina E foram administradas no 3º e 23º dias PI. Os testes in vivo mostraram aumento da longevidade nos grupos tratados com aceturato de diminazeno associado ao selenito de sódio (grupos F e H). Não foi encontrada diferença entre os grupos C e E, portanto, a vitamina E não aumentou a eficácia do tratamento contra T. evansi quando associado ao aceturato de diminazeno. A eficácia curativa dos tratamentos foi de 37.5, 87.7, 37.7 e 75% para os grupos C, F, G e H, respectivamente. Os demais tratamentos não mostraram eficácia. Assim, podemos sugerir que o selenito de sódio, quando combinado com a quimioterapia pode representar uma alternativa no tratamento da tripanossomose.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaRue, Mario Luiz de Lahttp://lattes.cnpq.br/5733736449142334Lopes, Sonia Teresinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Leal, Marta Lizandra do Rêgohttp://lattes.cnpq.br/6859797230596402Tonin, Alexandre Alberto2012-10-092012-10-092012-01-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfTONIN, Alexandre Alberto. Diminazene aceturate associated to sodium selenite and vitamin E: tests in vitro and in rats experimentally infected with Trypanosoma evansi. 2012. 79 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/8954ark:/26339/0013000014fk0porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-07-22T12:28:15Zoai:repositorio.ufsm.br:1/8954Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-07-22T12:28:15Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
Diminazene aceturate associated to sodium selenite and vitamin E: tests in vitro and in rats experimentally infected with Trypanosoma evansi
title Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
spellingShingle Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
Tonin, Alexandre Alberto
Trypanosoma evansi
Aceturato de diminazeno
Selenito de sódio
Vitamina E
Roedores
Trypanosoma evansi
Diminazene aceturate
Sodium selenite
Vitamin E
Rodents
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
title_full Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
title_fullStr Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
title_full_unstemmed Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
title_sort Aceturato de diminazeno associado ao selenito de sódio e a vitamina E: testes in vitro e em ratos experimentalmente infectados com Trypanosoma evansi
author Tonin, Alexandre Alberto
author_facet Tonin, Alexandre Alberto
author_role author
dc.contributor.none.fl_str_mv Rue, Mario Luiz de La
http://lattes.cnpq.br/5733736449142334
Lopes, Sonia Teresinha dos Anjos
http://lattes.cnpq.br/8059723754130756
Leal, Marta Lizandra do Rêgo
http://lattes.cnpq.br/6859797230596402
dc.contributor.author.fl_str_mv Tonin, Alexandre Alberto
dc.subject.por.fl_str_mv Trypanosoma evansi
Aceturato de diminazeno
Selenito de sódio
Vitamina E
Roedores
Trypanosoma evansi
Diminazene aceturate
Sodium selenite
Vitamin E
Rodents
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
topic Trypanosoma evansi
Aceturato de diminazeno
Selenito de sódio
Vitamina E
Roedores
Trypanosoma evansi
Diminazene aceturate
Sodium selenite
Vitamin E
Rodents
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description The aim of this study was to evaluate the utilization of a standard treatment with diminazene aceturate against the infection caused by T. evansi, associated to sodium selenite and vitamin E. In vitro tests showed trypanocidal effect related to the treatment with diminazene aceturate and sodium selenite, but vitamin E had no harmful effect on the trypanosomes. In vivo experiments utilized a total of 72 adult outbreed females rats, separated into 9 groups (A, B, C, D, E, F, G, H and I), 8 animals each. Group A was the uninfected group; groups B to I were infected with 0.2 mL of blood containing 106 trypanosomes. Parasitemia was estimated daily by microscopic examination of blood smears. Group B served as positive control; group C was treated with diminazene aceturate; group D with sodium selenite; group E with vitamin E; group F received an association of diminazene aceturate and sodium selenite; group G received an association of diminazene aceturate and vitamin E; group H received an association of diminazene aceturate, sodium selenite and vitamin E, and group I received an association of sodium selenite and vitamin E. Diminazene aceturate was administrated in a single dose on the 3rd day post infection (PI). Sodium selenite and vitamin E were administered at the 3rd and 23rd day PI. In vivo tests showed increase of longevity in groups treated with diminazene aceturate associated with sodium selenite (groups F and H). No difference was found between groups C and E, thus the vitamin E did not increase the efficacy of treatment against T. evansi when associated to diminazene aceturate. The curative efficacy of treatments was 37.5, 87.7, 37.7 and 75% to the groups C, F, G and H, respectively. Other treatments showed no efficacy. The sodium selenite when combined with chemotherapy may represent an alternative in the treatment of trypanosomosis.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-09
2012-10-09
2012-01-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv TONIN, Alexandre Alberto. Diminazene aceturate associated to sodium selenite and vitamin E: tests in vitro and in rats experimentally infected with Trypanosoma evansi. 2012. 79 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
http://repositorio.ufsm.br/handle/1/8954
dc.identifier.dark.fl_str_mv ark:/26339/0013000014fk0
identifier_str_mv TONIN, Alexandre Alberto. Diminazene aceturate associated to sodium selenite and vitamin E: tests in vitro and in rats experimentally infected with Trypanosoma evansi. 2012. 79 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
ark:/26339/0013000014fk0
url http://repositorio.ufsm.br/handle/1/8954
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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