Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)

Detalhes bibliográficos
Autor(a) principal: Fagundes, Joelma Dutra
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000mqzq
Texto Completo: http://repositorio.ufsm.br/handle/1/5116
Resumo: Aspilia montevidensis, considered a weed, presents an ornamental potential because of their exuberant flowers. Thus, in the future, through the characterization of the developmental and breeding programs, we may develop ornamental cultivars. The objectives of this dissertation are two-fold: determine base temperature, the plastocron, the final node number, and the duration of some phases of the developmental cycle from A. montevidensis (Chapter I) and to verify seed production and to evaluate the germination of seeds collected from different locations, to evaluate in vitro establishment from A. montevidensis nodal segments from plants of two populations, and to evaluate in vitro multiplication of the population that had the best performance in the establishment (Chapter II). Two experiments were conducted at the experimental field, in the Production Seeds Analysis Laboratory and in the Biotechnology Laboratory of the Federal University of Santa Maria, RS, Brazil. In the first experiment, five planting dates in the field (20/12/2005, 14/02/2006, 06/03/2006, 04/04/2006, 05/05/2006) and one sowing date in a greenhouse (08/06/2006) were used. The date of developmental stages transplanting (T), visible buds (VB), open capitulum (OC) and senescent capitulum (SC), and the number of visible nodes were measured on the maim stem (MS) and on first and secondary lateral branches located on two positions on the mother stem (lower node and upper node) of each plant per pot. The base temperature (Tb) for node appearance and Tb of the T-VB and VB-SC phases was estimated with three empirical methods. The daily thermal time (TTd, °C day) was calculated from transplanting and the plastochron (ºC day node-1) was estimated for each stem. In the second experiment, Capitulum diameter was measured on 45 samples from three populations and the number of viable seeds, aborted seeds and the average seed weight were determined. Germination of seeds was also tested at 20ºC and 30ºC. For in vitro establishment, a MS media and nodal segments from two populations were used, and the following parameters were evaluated: contamination (C), survival (S), establishment (E), total number of nodes (TN), leaf number (LN), presence of lateral buds (PLB), number lateral buds (NLB) and presence of roots (PR). The in vitro treatments were MS, media MS + 1mg L-1 BAP, and MS + 1mg L-1 BAP + 0,01mg L-1 ANA and the parameters evaluated were TN, LN, PLB, NLB, PR, and presence of calluses (PC).The estimated base temperature for node appearance in A. montevidensis was 0ºC and Tb of the T-VB and VB-SC phases was 13ºC e 7ºC, respectively. Plastochron and final node number (FNN) varied among stems and planting dates, with the lowest plastochron and the greatest FNN on the MS. A duration, in ºC day, of the T-VB phase varied among stems and planting dates whereas the duration of VB-OC and OC-SC, in general did not vary among stems and planting dates. Differences in the number and quality of the seeds from different sampling locations allowed to infer that the difference in environment among locations affected the seed production of A. montevidensis. The best temperature for seeds germination is 20ºC. Nodal segments from plants depressed by endogamy presented a poorer in vitro establishment. The media indicated for multiplication is the MS media plus 1mg L-1 BAP and 0,01mg L-1 ANA.
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spelling Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)Quantifying of development and pre-breeding of aspilia montevidensis (Spreng.)Aspilia montevidensisFenologiaPropagaçãoAspilia montevidensisPhenologyPropagationCNPQ::CIENCIAS AGRARIAS::AGRONOMIAAspilia montevidensis, considered a weed, presents an ornamental potential because of their exuberant flowers. Thus, in the future, through the characterization of the developmental and breeding programs, we may develop ornamental cultivars. The objectives of this dissertation are two-fold: determine base temperature, the plastocron, the final node number, and the duration of some phases of the developmental cycle from A. montevidensis (Chapter I) and to verify seed production and to evaluate the germination of seeds collected from different locations, to evaluate in vitro establishment from A. montevidensis nodal segments from plants of two populations, and to evaluate in vitro multiplication of the population that had the best performance in the establishment (Chapter II). Two experiments were conducted at the experimental field, in the Production Seeds Analysis Laboratory and in the Biotechnology Laboratory of the Federal University of Santa Maria, RS, Brazil. In the first experiment, five planting dates in the field (20/12/2005, 14/02/2006, 06/03/2006, 04/04/2006, 05/05/2006) and one sowing date in a greenhouse (08/06/2006) were used. The date of developmental stages transplanting (T), visible buds (VB), open capitulum (OC) and senescent capitulum (SC), and the number of visible nodes were measured on the maim stem (MS) and on first and secondary lateral branches located on two positions on the mother stem (lower node and upper node) of each plant per pot. The base temperature (Tb) for node appearance and Tb of the T-VB and VB-SC phases was estimated with three empirical methods. The daily thermal time (TTd, °C day) was calculated from transplanting and the plastochron (ºC day node-1) was estimated for each stem. In the second experiment, Capitulum diameter was measured on 45 samples from three populations and the number of viable seeds, aborted seeds and the average seed weight were determined. Germination of seeds was also tested at 20ºC and 30ºC. For in vitro establishment, a MS media and nodal segments from two populations were used, and the following parameters were evaluated: contamination (C), survival (S), establishment (E), total number of nodes (TN), leaf number (LN), presence of lateral buds (PLB), number lateral buds (NLB) and presence of roots (PR). The in vitro treatments were MS, media MS + 1mg L-1 BAP, and MS + 1mg L-1 BAP + 0,01mg L-1 ANA and the parameters evaluated were TN, LN, PLB, NLB, PR, and presence of calluses (PC).The estimated base temperature for node appearance in A. montevidensis was 0ºC and Tb of the T-VB and VB-SC phases was 13ºC e 7ºC, respectively. Plastochron and final node number (FNN) varied among stems and planting dates, with the lowest plastochron and the greatest FNN on the MS. A duration, in ºC day, of the T-VB phase varied among stems and planting dates whereas the duration of VB-OC and OC-SC, in general did not vary among stems and planting dates. Differences in the number and quality of the seeds from different sampling locations allowed to infer that the difference in environment among locations affected the seed production of A. montevidensis. The best temperature for seeds germination is 20ºC. Nodal segments from plants depressed by endogamy presented a poorer in vitro establishment. The media indicated for multiplication is the MS media plus 1mg L-1 BAP and 0,01mg L-1 ANA.Aspilia montevidensis, considerada uma planta daninha, devido a grande exuberância de suas flores apresenta um potencial ornamental. Assim, no futuro, através da caracterização do desenvolvimento e programas de melhoramento genético pode-se chegar a cultivares ornamentais. Os objetivos da dissertação foram: Determinar a temperatura base, o plastocrono, o número final de nós e a duração de algumas fases do ciclo de desenvolvimento de A. montevidensis (capítulo I) e verificar a produção de sementes e avaliar a germinação de sementes coletadas em diferentes populações, avaliar o estabelecimento in vitro a partir de segmentos nodais de A. montevidensis provenientes de plantas de duas populações e avaliar a multiplicação in vitro da população que obteve melhor desempenho no estabelecimento (capítulo II). Dois experimentos foram conduzidos no campo experimental, no Laboratório de Analise de Sementes de Produção (LASP) e no Laboratório de Biotecnologia e Melhoramento da Universidade Federal de Santa Maria. No primeiro experimento foram realizadas cinco épocas de cultivo a campo (20/12/2005, 14/02/2006, 06/03/2006, 04/04/2006, 05/05/2006) e uma em casa de vegetação (08/06/2006). As datas dos estágios de desenvolvimento transplante (T), botão visível (BV), capítulo aberto (CA) e capítulo senescente (CS), e o número de nós visíveis foram registradas na haste principal (HP) e em hastes laterais. A temperatura base (Tb) de emissão de nós foi determinada pelo método do menor QME e a Tb das fases T-BV e BV-CS foi estimada por três métodos. A soma térmica diária (STd, °C dia) foi calculada a partir do transplante e o plastocrono (ºC dia nó-1) foi estimado. No segundo experimento foi medido o diâmetro de 45 capítulos coletados em três populações sendo contado o número de sementes viáveis e abortadas e verificada a massa média das sementes viáveis. Foram realizados também testes de germinação a 20ºC e 30ºC. No estabelecimento in vitro foi utilizado meio MS e segmentos nodais provenientes de duas populações sendo avaliados presença de contaminação (PCT), sobrevivência (S), estabelecimento (E), número total de nós (NT), número de folhas (NF), presença de gemas laterais (PGL), número de gemas laterais (NGL) e presença de raízes (PR). Na multiplicação in vitro os tratamentos utilizados foram: meio MS, MS + 1 mg L-1 BAP e MS + 1 mg L-1 BAP + 0,01 mg L-1 ANA sendo avaliados NT, NF, PGL, NGL, PR e presença de calos (PC). A temperatura base estimada para emissão nós de A. montevidensis foi 0ºC e a Tb das fases T-BV e BV-CS foi 13ºC e 7ºC, respectivamente. O plastocrono e o número final de nós (NFN) variaram entre hastes e épocas de cultivo, sendo os menores valores de plastocrono e maiores valores de NFN observados na HP. A duração em ºC dia da fase T-BV variou entre hastes e épocas de cultivo enquanto que a duração das fases BV-CA e CA-CS, em geral não variou entre hastes e épocas. As diferenças encontradas no número e qualidade das sementes das distintas populações permitem verificar que a diferença entre os locais interfere diretamente na produção de semente de A. montevidensis. A temperatura de 20ºC é apropriada para a germinação de sementes de A. montevidensis. Segmentos nodais provenientes de plantas deprimidas pela endogamia apresentam um estabelecimento in vitro inferior. O meio indicado para multiplicação de A. montevidensis é o meio MS suplementado com 1mg L-1 de BAP associado a 0,01mg L-1 de ANA.Universidade Federal de Santa MariaBRAgronomiaUFSMPrograma de Pós-Graduação em AgronomiaStreck, Nereu Augustohttp://lattes.cnpq.br/8121082379157248Reiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391Streck, Lucianohttp://lattes.cnpq.br/2108914472894768Fagundes, Joelma Dutra2017-05-092017-05-092007-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFAGUNDES, Joelma Dutra. Quantifying of development and pre-breeding of aspilia montevidensis (Spreng.). 2007. 73 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2007.http://repositorio.ufsm.br/handle/1/5116ark:/26339/001300000mqzqporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-01-06T16:51:31Zoai:repositorio.ufsm.br:1/5116Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:45:38.577549Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
Quantifying of development and pre-breeding of aspilia montevidensis (Spreng.)
title Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
spellingShingle Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
Fagundes, Joelma Dutra
Aspilia montevidensis
Fenologia
Propagação
Aspilia montevidensis
Phenology
Propagation
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
title_short Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
title_full Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
title_fullStr Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
title_full_unstemmed Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
title_sort Quantificação do desenvolvimento e pré-melhoramento de Aspilia montevidensis (Spreng.)
author Fagundes, Joelma Dutra
author_facet Fagundes, Joelma Dutra
author_role author
dc.contributor.none.fl_str_mv Streck, Nereu Augusto
http://lattes.cnpq.br/8121082379157248
Reiniger, Lia Rejane Silveira
http://lattes.cnpq.br/5739294882585391
Streck, Luciano
http://lattes.cnpq.br/2108914472894768
dc.contributor.author.fl_str_mv Fagundes, Joelma Dutra
dc.subject.por.fl_str_mv Aspilia montevidensis
Fenologia
Propagação
Aspilia montevidensis
Phenology
Propagation
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
topic Aspilia montevidensis
Fenologia
Propagação
Aspilia montevidensis
Phenology
Propagation
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
description Aspilia montevidensis, considered a weed, presents an ornamental potential because of their exuberant flowers. Thus, in the future, through the characterization of the developmental and breeding programs, we may develop ornamental cultivars. The objectives of this dissertation are two-fold: determine base temperature, the plastocron, the final node number, and the duration of some phases of the developmental cycle from A. montevidensis (Chapter I) and to verify seed production and to evaluate the germination of seeds collected from different locations, to evaluate in vitro establishment from A. montevidensis nodal segments from plants of two populations, and to evaluate in vitro multiplication of the population that had the best performance in the establishment (Chapter II). Two experiments were conducted at the experimental field, in the Production Seeds Analysis Laboratory and in the Biotechnology Laboratory of the Federal University of Santa Maria, RS, Brazil. In the first experiment, five planting dates in the field (20/12/2005, 14/02/2006, 06/03/2006, 04/04/2006, 05/05/2006) and one sowing date in a greenhouse (08/06/2006) were used. The date of developmental stages transplanting (T), visible buds (VB), open capitulum (OC) and senescent capitulum (SC), and the number of visible nodes were measured on the maim stem (MS) and on first and secondary lateral branches located on two positions on the mother stem (lower node and upper node) of each plant per pot. The base temperature (Tb) for node appearance and Tb of the T-VB and VB-SC phases was estimated with three empirical methods. The daily thermal time (TTd, °C day) was calculated from transplanting and the plastochron (ºC day node-1) was estimated for each stem. In the second experiment, Capitulum diameter was measured on 45 samples from three populations and the number of viable seeds, aborted seeds and the average seed weight were determined. Germination of seeds was also tested at 20ºC and 30ºC. For in vitro establishment, a MS media and nodal segments from two populations were used, and the following parameters were evaluated: contamination (C), survival (S), establishment (E), total number of nodes (TN), leaf number (LN), presence of lateral buds (PLB), number lateral buds (NLB) and presence of roots (PR). The in vitro treatments were MS, media MS + 1mg L-1 BAP, and MS + 1mg L-1 BAP + 0,01mg L-1 ANA and the parameters evaluated were TN, LN, PLB, NLB, PR, and presence of calluses (PC).The estimated base temperature for node appearance in A. montevidensis was 0ºC and Tb of the T-VB and VB-SC phases was 13ºC e 7ºC, respectively. Plastochron and final node number (FNN) varied among stems and planting dates, with the lowest plastochron and the greatest FNN on the MS. A duration, in ºC day, of the T-VB phase varied among stems and planting dates whereas the duration of VB-OC and OC-SC, in general did not vary among stems and planting dates. Differences in the number and quality of the seeds from different sampling locations allowed to infer that the difference in environment among locations affected the seed production of A. montevidensis. The best temperature for seeds germination is 20ºC. Nodal segments from plants depressed by endogamy presented a poorer in vitro establishment. The media indicated for multiplication is the MS media plus 1mg L-1 BAP and 0,01mg L-1 ANA.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-27
2017-05-09
2017-05-09
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv FAGUNDES, Joelma Dutra. Quantifying of development and pre-breeding of aspilia montevidensis (Spreng.). 2007. 73 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2007.
http://repositorio.ufsm.br/handle/1/5116
dc.identifier.dark.fl_str_mv ark:/26339/001300000mqzq
identifier_str_mv FAGUNDES, Joelma Dutra. Quantifying of development and pre-breeding of aspilia montevidensis (Spreng.). 2007. 73 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2007.
ark:/26339/001300000mqzq
url http://repositorio.ufsm.br/handle/1/5116
dc.language.iso.fl_str_mv por
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dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Agronomia
UFSM
Programa de Pós-Graduação em Agronomia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Agronomia
UFSM
Programa de Pós-Graduação em Agronomia
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
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instname_str Universidade Federal de Santa Maria (UFSM)
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institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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