Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/3189 |
Resumo: | Trichoderma spp. is at the present time, one of the fungi most studied as biocontrol agent, being antagonistic to several pathogens in different crops, besides promoting vegetable growth. The pathogen Fusarium oxysporum causes the fusarial wilt and is difficult to control mainly because of its capacity to remain in the soil for long periods, even without the presence of the host. Studies of the population dynamics of Trichoderma spp., Fusarium spp. and native microorganisms are necessary, mainly to observe the impact of the biocontrol agent addition to the soil. Vegetables as cucumber and tomato are commonly cultivated in South Brazil and are sensitive to a variety of diseases, as the fusarial wilt which is one of, their main diseases, specially in protected environment. For the biological control of the fusarial wilt in the cultivation of tomato and cucumber by Trichoderma and to study the population dynamics of the biocontrol agent when added to the soil, the following objectives were proposed: to study the space distribution of both Trichoderma and Fusarium in rhizosphere and bulk soil, in the cultivation of cucumber and tomato, in vegetable garden and greenhouse; to find a method for the mass selection of fungi isolates antagonistic to Fusarium solani and Fusarium oxysporum in microenvironment, and to identify the more effective ones; to verify the action of Trichoderma harzianum isolates in the development and protection of seedlings against the fusarial wilt of tomato and cucumer, identifying the best form of the biocontrol agent application in the control of the fusarial wilt; to analyze the population dynamics of Trichoderma and Fusarium, and of part of the microorganisms of the soil, with the addition of the biocontrol agents, in the cultivation of the tomato and cucumber, in greenhouse and vegetable garden. Both Trichoderma and Fusarium were found in the rhizosphere and bulk soil and the rhizosphere soil had the greater frequence of Trichoderma specially in greenhouse. The method of mass selection, in substrat, of fungi isolates antagonistic to the pathogens F. solani and F. oxysporum was efficient, since the three isolates of Trichoderma harzianum selected, besides reducing the population of the pathogens in substrate were also effective against the fusarial wilt of tomato and cucumber when applied as seed or substrate treatment. A population flutuation of Trichoderma spp. ocurred when isolates of T. harzianum were added to the soil in the cultivation of tomato and cucumber, in vegetable garden and greenhouse. There was a tendency of the population of Trichoderma spp. to return to the initial parameters, i.e. before the biocontrole agent was added to the soil. The population of bacteria, Fusarium spp. and general fungi did not show great alterations with the presence of the isolates of the biocontrol agent. |
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2017-05-112017-05-112006-01-13ETHUR, Luciana Zago. Population dynamics and action of trichoderma in the control of fusarial wilt of tomato and cucumber seedlings.. 2006. 155 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/3189Trichoderma spp. is at the present time, one of the fungi most studied as biocontrol agent, being antagonistic to several pathogens in different crops, besides promoting vegetable growth. The pathogen Fusarium oxysporum causes the fusarial wilt and is difficult to control mainly because of its capacity to remain in the soil for long periods, even without the presence of the host. Studies of the population dynamics of Trichoderma spp., Fusarium spp. and native microorganisms are necessary, mainly to observe the impact of the biocontrol agent addition to the soil. Vegetables as cucumber and tomato are commonly cultivated in South Brazil and are sensitive to a variety of diseases, as the fusarial wilt which is one of, their main diseases, specially in protected environment. For the biological control of the fusarial wilt in the cultivation of tomato and cucumber by Trichoderma and to study the population dynamics of the biocontrol agent when added to the soil, the following objectives were proposed: to study the space distribution of both Trichoderma and Fusarium in rhizosphere and bulk soil, in the cultivation of cucumber and tomato, in vegetable garden and greenhouse; to find a method for the mass selection of fungi isolates antagonistic to Fusarium solani and Fusarium oxysporum in microenvironment, and to identify the more effective ones; to verify the action of Trichoderma harzianum isolates in the development and protection of seedlings against the fusarial wilt of tomato and cucumer, identifying the best form of the biocontrol agent application in the control of the fusarial wilt; to analyze the population dynamics of Trichoderma and Fusarium, and of part of the microorganisms of the soil, with the addition of the biocontrol agents, in the cultivation of the tomato and cucumber, in greenhouse and vegetable garden. Both Trichoderma and Fusarium were found in the rhizosphere and bulk soil and the rhizosphere soil had the greater frequence of Trichoderma specially in greenhouse. The method of mass selection, in substrat, of fungi isolates antagonistic to the pathogens F. solani and F. oxysporum was efficient, since the three isolates of Trichoderma harzianum selected, besides reducing the population of the pathogens in substrate were also effective against the fusarial wilt of tomato and cucumber when applied as seed or substrate treatment. A population flutuation of Trichoderma spp. ocurred when isolates of T. harzianum were added to the soil in the cultivation of tomato and cucumber, in vegetable garden and greenhouse. There was a tendency of the population of Trichoderma spp. to return to the initial parameters, i.e. before the biocontrole agent was added to the soil. The population of bacteria, Fusarium spp. and general fungi did not show great alterations with the presence of the isolates of the biocontrol agent.Trichoderma spp. é um dos fungos mais pesquisados na atualidade como agente de biocontrole, sendo antagonista a vários fitopatógenos em diferentes culturas, além de auxiliar no desenvolvimento vegetal. O patógeno Fusarium oxysporum causador da murcha de fusário ou fusariose é difícil de ser controlado, principalmente pela sua capacidade em manter-se no solo por longos períodos, mesmo sem a presença do hospedeiro. Estudos da dinâmica populacional de Trichoderma spp., Fusarium spp. e microbiota nativa são necessários, principalmente para observar-se o impacto da adição do agente de biocontrole no ambiente solo. Olerícolas, como pepineiro e tomateiro, são comumente cultivadas na Região Sul, porém são sensíveis a uma gama de doenças, dentre as quais, a fusariose, que figura como principal doença, principalmente em ambiente protegido. Para buscar o controle biológico da fusariose no cultivo do tomateiro e pepineiro por Trichoderma e estudar a dinâmica populacional desse agente de biocontrole quando adicionado ao solo, foram propostos os seguintes objetivos: estudar a distribuição espacial dos gêneros Trichoderma e Fusarium em solo rizosférico e não rizosférico, no cultivo do pepineiro e tomateiro, em horta e estufa; utilizar micro-ambiente para realizar a seleção massal de isolados fúngicos antagônicos a Fusarium solani e Fusarium oxysporum e identificar, em nível de espécie, os isolados fúngicos mais efetivos; verificar a ação de isolados de Trichoderma harzianum no desenvolvimento e na proteção de mudas contra a murcha de fusário do tomateiro e do pepineiro, identificando a melhor forma de aplicação do agente de biocontrole no controle da fusariose; analisar a dinâmica populacional dos gêneros Trichoderma e Fusarium e de parte da microbiota do solo, com a adição de agente de biocontrole, no cultivo do tomateiro e pepineiro, em estufa e horta. Tanto Trichoderma quanto Fusarium foram encontrados em solo rizosférico e não rizosférico de horta e estufa, sendo que Trichoderma foi encontrado em maior número de coletas em solo rizosférico de estufa. O método de seleção massal, em substrato, de isolados fúngicos antagônicos aos patógenos F. solani e F. oxysporum foi eficiente, pois os três isolados de Trichoderma harzianum selecionados, além de reduzirem a população dos patógenos em substrato, foram efetivos contra a fusariose do tomateiro e pepineiro, em tratamento de semente ou de substrato. Ocorreu flutuação populacional de Trichoderma spp. quando isolados de T. harzianum foram adicionados ao solo no cultivo do tomateiro e pepineiro, em horta e estufa. Observou-se uma tendência da população de Trichoderma spp. voltar aos parâmetros iniciais, ou seja, antes do agente de biocontrole ser adicionado ao ambiente solo. A população de bactérias, Fusarium spp. e fungos gerais não apresentou grandes alterações pela presença dos isolados do agente de biocontrole.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgronomiaUFSMBRAgronomiaFusarium oxysporumEstufaRizosferaControle biológicoFusarium oxysporumGreenhouseRhizosphereBiological controlCNPQ::CIENCIAS AGRARIAS::AGRONOMIADinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiroPopulation dynamics and action of trichoderma in the control of fusarial wilt of tomato and cucumber seedlings.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisBlume, Elenahttp://lattes.cnpq.br/3299996746493028Antoniolli, Zaida Inêshttp://lattes.cnpq.br/4692942549618168Silva, Antonio Carlos Ferreira dahttp://lattes.cnpq.br/9520096764579199Muniz, Marlove Fátima Briãohttp://lattes.cnpq.br/3148312031889388http://lattes.cnpq.br/3040963753794741Ethur, Luciana Zago500100000009400300300300300300dd9a87c1-c361-458f-b4bd-b78407907de8fbd34cd5-f75a-48a4-8582-f6e64c16da2e9b2ce951-cf6a-485d-8f03-636e839d89d111e6952b-8c0f-44ca-a865-e773e1e6b164c81de519-87d6-41ed-9a7a-9525797bc8adinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTese - Luciana Zago Ethur.pdfapplication/pdf1181466http://repositorio.ufsm.br/bitstream/1/3189/1/Tese%20-%20Luciana%20Zago%20Ethur.pdfa6d58c296e6c2b40a3b78324546c7f60MD51TEXTTese - Luciana Zago Ethur.pdf.txtTese - Luciana Zago Ethur.pdf.txtExtracted texttext/plain308206http://repositorio.ufsm.br/bitstream/1/3189/2/Tese%20-%20Luciana%20Zago%20Ethur.pdf.txt106122ae70f908187e3df701e4b97184MD52THUMBNAILTese - Luciana Zago Ethur.pdf.jpgTese - Luciana Zago Ethur.pdf.jpgIM Thumbnailimage/jpeg4775http://repositorio.ufsm.br/bitstream/1/3189/3/Tese%20-%20Luciana%20Zago%20Ethur.pdf.jpge52adad74bd2d126d2174f377dee2837MD531/31892023-01-05 13:52:24.325oai:repositorio.ufsm.br:1/3189Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-01-05T16:52:24Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
dc.title.alternative.eng.fl_str_mv |
Population dynamics and action of trichoderma in the control of fusarial wilt of tomato and cucumber seedlings. |
title |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
spellingShingle |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro Ethur, Luciana Zago Fusarium oxysporum Estufa Rizosfera Controle biológico Fusarium oxysporum Greenhouse Rhizosphere Biological control CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
title_full |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
title_fullStr |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
title_full_unstemmed |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
title_sort |
Dinâmica populacional e ação de trichoderma no controle de fusariose em mudas de tomateiro e pepineiro |
author |
Ethur, Luciana Zago |
author_facet |
Ethur, Luciana Zago |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Blume, Elena |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3299996746493028 |
dc.contributor.referee1.fl_str_mv |
Antoniolli, Zaida Inês |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4692942549618168 |
dc.contributor.referee2.fl_str_mv |
Silva, Antonio Carlos Ferreira da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9520096764579199 |
dc.contributor.referee3.fl_str_mv |
Muniz, Marlove Fátima Brião |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/3148312031889388 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3040963753794741 |
dc.contributor.author.fl_str_mv |
Ethur, Luciana Zago |
contributor_str_mv |
Blume, Elena Antoniolli, Zaida Inês Silva, Antonio Carlos Ferreira da Muniz, Marlove Fátima Brião |
dc.subject.por.fl_str_mv |
Fusarium oxysporum Estufa Rizosfera Controle biológico |
topic |
Fusarium oxysporum Estufa Rizosfera Controle biológico Fusarium oxysporum Greenhouse Rhizosphere Biological control CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Fusarium oxysporum Greenhouse Rhizosphere Biological control |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Trichoderma spp. is at the present time, one of the fungi most studied as biocontrol agent, being antagonistic to several pathogens in different crops, besides promoting vegetable growth. The pathogen Fusarium oxysporum causes the fusarial wilt and is difficult to control mainly because of its capacity to remain in the soil for long periods, even without the presence of the host. Studies of the population dynamics of Trichoderma spp., Fusarium spp. and native microorganisms are necessary, mainly to observe the impact of the biocontrol agent addition to the soil. Vegetables as cucumber and tomato are commonly cultivated in South Brazil and are sensitive to a variety of diseases, as the fusarial wilt which is one of, their main diseases, specially in protected environment. For the biological control of the fusarial wilt in the cultivation of tomato and cucumber by Trichoderma and to study the population dynamics of the biocontrol agent when added to the soil, the following objectives were proposed: to study the space distribution of both Trichoderma and Fusarium in rhizosphere and bulk soil, in the cultivation of cucumber and tomato, in vegetable garden and greenhouse; to find a method for the mass selection of fungi isolates antagonistic to Fusarium solani and Fusarium oxysporum in microenvironment, and to identify the more effective ones; to verify the action of Trichoderma harzianum isolates in the development and protection of seedlings against the fusarial wilt of tomato and cucumer, identifying the best form of the biocontrol agent application in the control of the fusarial wilt; to analyze the population dynamics of Trichoderma and Fusarium, and of part of the microorganisms of the soil, with the addition of the biocontrol agents, in the cultivation of the tomato and cucumber, in greenhouse and vegetable garden. Both Trichoderma and Fusarium were found in the rhizosphere and bulk soil and the rhizosphere soil had the greater frequence of Trichoderma specially in greenhouse. The method of mass selection, in substrat, of fungi isolates antagonistic to the pathogens F. solani and F. oxysporum was efficient, since the three isolates of Trichoderma harzianum selected, besides reducing the population of the pathogens in substrate were also effective against the fusarial wilt of tomato and cucumber when applied as seed or substrate treatment. A population flutuation of Trichoderma spp. ocurred when isolates of T. harzianum were added to the soil in the cultivation of tomato and cucumber, in vegetable garden and greenhouse. There was a tendency of the population of Trichoderma spp. to return to the initial parameters, i.e. before the biocontrole agent was added to the soil. The population of bacteria, Fusarium spp. and general fungi did not show great alterations with the presence of the isolates of the biocontrol agent. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-01-13 |
dc.date.accessioned.fl_str_mv |
2017-05-11 |
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2017-05-11 |
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info:eu-repo/semantics/doctoralThesis |
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ETHUR, Luciana Zago. Population dynamics and action of trichoderma in the control of fusarial wilt of tomato and cucumber seedlings.. 2006. 155 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2006. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/3189 |
identifier_str_mv |
ETHUR, Luciana Zago. Population dynamics and action of trichoderma in the control of fusarial wilt of tomato and cucumber seedlings.. 2006. 155 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2006. |
url |
http://repositorio.ufsm.br/handle/1/3189 |
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Universidade Federal de Santa Maria |
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UFSM |
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Universidade Federal de Santa Maria |
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