Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/9026 |
Resumo: | This study aimed the evaluation of the trypanocidal activity of terpinen-4-ol, γ-terpinene and α-terpinene against Trypanosoma evansi in vivo and in vitro. The in vitro assay was performed in culture medium of T. evansi, using three different concentrations of each compound (0.5, 1.0 and 2.0%), individually and in synergism. Motile (live) trypomastigotes were counted using a Neubauer chamber after 1, 3, 6 and 9 hours of incubation. Death of parasites occurred in a concentration-dependent manner, except for α-terpinene that was able to eliminate all parasites after 1 hour of incubation at all concentrations tested. Seventy mice were equally assigned into 10 groups (A to J) for the in vivo tests. Group A served as a negative control (healthy animals); groups B - J were infected with 0.1 mL of blood containing 105 T. evansi trypomastigotes. Group B served as a positive control; groups C, D and E received oral doses of 1.0 mL kg-1 of terpinen-4-ol, γ-terpinene and α-terpinene, respectively. Associations of the three compounds were orally administered to groups F I: terpinen-4-ol + γ-terpinene to group F, terpinen-4-ol + α-terpinene to group G, α-terpinene + γ-terpinene at 1.0 mL kg-1 (0.5 mL kg-1 for each compound) to group H, terpinen-4-ol + γ-terpinene + α-terpinene at 1.0 mL kg-1 (0.335 mL kg-1 of each compound) to group I. A therapeutic dose of diminazene aceturate (single dose of 3.5 mg kg-1, intraperitoneally) was administered to Group J aiming test validation. Animals in group E (treated with α-terpinene) showed an increase of longevity when compared to animals in group B. However, no curative effectiveness was observed, since all animals in this group died during the observation period. In order to evaluate the association between α-terpinene and diminazene aceturate, twenty-eight mice were assigned into 4 groups (A, B, C and D, n = 7). Group A included animals uninfected (negative control); group B was composed by infected animals (positive control); group C consisted of infected animals treated with diminazene aceturate (single dose of 3.5 mg kg-1, im); and group D consisted of infected animals treated with an association of diminazene aceturate (single dose of 3.5 mg kg-1, im) and α-terpinene (1.0 ml kg-1, orally administered for 10 days). It was observed, as a result of this experiment, an increase in the curative effectiveness (57.14%) in group D when compared to group C, which showed only 14.28% of curative effectiveness. Based on these results, α-terpinene was the compound present in M. alternifolia essential oil that showed trypanocidal activity. |
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2016-05-022016-05-022016-02-15BALDISSERA, Matheus Dellaméa. EVALUATION OF THE ACTIVITY OF THREE COMPOUNDS OF MELALEUCA OIL AGAINST Trypanosoma evansi. 2016. 64 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/9026This study aimed the evaluation of the trypanocidal activity of terpinen-4-ol, γ-terpinene and α-terpinene against Trypanosoma evansi in vivo and in vitro. The in vitro assay was performed in culture medium of T. evansi, using three different concentrations of each compound (0.5, 1.0 and 2.0%), individually and in synergism. Motile (live) trypomastigotes were counted using a Neubauer chamber after 1, 3, 6 and 9 hours of incubation. Death of parasites occurred in a concentration-dependent manner, except for α-terpinene that was able to eliminate all parasites after 1 hour of incubation at all concentrations tested. Seventy mice were equally assigned into 10 groups (A to J) for the in vivo tests. Group A served as a negative control (healthy animals); groups B - J were infected with 0.1 mL of blood containing 105 T. evansi trypomastigotes. Group B served as a positive control; groups C, D and E received oral doses of 1.0 mL kg-1 of terpinen-4-ol, γ-terpinene and α-terpinene, respectively. Associations of the three compounds were orally administered to groups F I: terpinen-4-ol + γ-terpinene to group F, terpinen-4-ol + α-terpinene to group G, α-terpinene + γ-terpinene at 1.0 mL kg-1 (0.5 mL kg-1 for each compound) to group H, terpinen-4-ol + γ-terpinene + α-terpinene at 1.0 mL kg-1 (0.335 mL kg-1 of each compound) to group I. A therapeutic dose of diminazene aceturate (single dose of 3.5 mg kg-1, intraperitoneally) was administered to Group J aiming test validation. Animals in group E (treated with α-terpinene) showed an increase of longevity when compared to animals in group B. However, no curative effectiveness was observed, since all animals in this group died during the observation period. In order to evaluate the association between α-terpinene and diminazene aceturate, twenty-eight mice were assigned into 4 groups (A, B, C and D, n = 7). Group A included animals uninfected (negative control); group B was composed by infected animals (positive control); group C consisted of infected animals treated with diminazene aceturate (single dose of 3.5 mg kg-1, im); and group D consisted of infected animals treated with an association of diminazene aceturate (single dose of 3.5 mg kg-1, im) and α-terpinene (1.0 ml kg-1, orally administered for 10 days). It was observed, as a result of this experiment, an increase in the curative effectiveness (57.14%) in group D when compared to group C, which showed only 14.28% of curative effectiveness. Based on these results, α-terpinene was the compound present in M. alternifolia essential oil that showed trypanocidal activity.O objetivo deste estudo foi avaliar a atividade tripanocida do terpinen-4-ol, gama-(γ)-terpinen e alfa-(α)-terpinen contra o Trypanosoma evansi in vitro e in vivo. Os testes in vitro foram realizados em meio de cultura contendo T. evansi, utilizando-se três concentrações de cada composto (0.5, 1.0 e 2.0%) de forma individual e em associação. A contagem de tripomastigotas vivas foi realizada em câmara de Neubauer após 1, 3, 6 e 9 horas pós-incubação. A partir dos testes in vitro, foi possível observar a morte dos parasitos de forma concentração-dependente, exceto o composto α-terpinen que foi capaz de eliminar todos os parasitos 1 h pós-incubação em todas as concentrações testadas. Para realização dos ensaios in vivo, primeiramente foram utilizados 70 camundongos divididos em 10 grupos (A ao J), com sete animais cada grupo. O grupo A serviu como controle negativo (animais sadios); grupos de B a J foram infectados com 0,1 mL de sangue contendo 105 tripomastigotas com T. evansi. O grupo B serviu como controle positivo; grupos C, D e E receberam terpinen-4-ol, γ-terpinen e α-terpinen via oral na dose de 1.0 mL kg-1, respectivamente. O grupo F recebeu a associação dos compostos terpinen-4-ol + γ-terpinen, grupo G recebeu a associação dos compostos terpinen-4-ol + α-terpinen; grupo H recebeu a associação do γ-terpinen + α-terpinen via oral na dose de 1.0 mL kg-1 (0.5 mL kg-1 de cada composto). O grupo I recebeu a associação dos compostos terpinen-4-ol + γ-terpinen + α-terpinen via oral na dose de 1.0 mL kg-1 (0.335 mL kg-1 de cada composto). O grupo J foi tratado com aceturato de diminazeno na dose terapêutica (dose única de 3.5 mg kg-1, via intramuscular) para validação do teste. Foi possível verificar que os animais do grupo E (tratados com α-terpinen) apresentaram um aumento da longevidade quando comparados ao grupo B, porém sem eficácia curativa, pois todos os animais deste grupo morreram durante o período de observação da parasitemia. A partir disto, um segundo experimento foi realizado testando a associação entre o α-terpinen e o aceturato de diminazeno. Foram utilizados 28 camundongos divididos em 4 grupos (A, B, C e D; n=7). O grupo A foi formado por animais não infectados (controle negativo), o grupo B foi formado por animais infectados (controle positivo), o grupo C foi formado por animais infectados e tratados com aceturato de diminazeno (dose única de 3.5 mg kg-1, via intramuscular), e o grupo D foi formado por animais infectados e tratados com associação de aceturato de diminazeno (dose única de 3.5 mg kg-1, via intramuscular) mais α-terpinen (1.0 mL kg-1 via oral durante 10 dias). A partir deste resultado, foi possível observar um aumento da eficácia curativa (57.14 %) no grupo D quando comparado ao grupo C, que obteve apenas 14.28 % de eficácia curativa. Baseado nestes resultados, o α-terpinen foi o composto presente no óleo essencial de M. alternifolia que apresentou atividade tripanocida.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em FarmacologiaUFSMBRFarmacologiaMelaleuca alternifoliaSurraPlantas medicinaisTripanossomoseMelaleuca alternifoliaSurraMedicinal plantsTripanosomiasisCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAAvaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansiEvaluation of the activity of three compounds of melaleuca oil against Trypanosoma evansiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMonteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Sangioni, Luis Antoniohttp://lattes.cnpq.br/8056805667740451Parodi, Thaylise Veyhttp://lattes.cnpq.br/4183167775476307http://lattes.cnpq.br/5978984877357023Baldissera, Matheus Dellaméa201000000000400500300500300f658c142-7522-4cd5-a133-f2479652bf31ce163bcb-3890-4862-8211-907911b3a0a8a84ddba0-2c33-4160-a207-1e022964720df6788387-c192-4e3e-95ad-45969bfc398einfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBALDISSERA, MATHEUS DELLAMEA.pdfapplication/pdf7162139http://repositorio.ufsm.br/bitstream/1/9026/1/BALDISSERA%2c%20MATHEUS%20DELLAMEA.pdf4f32d77f8b7039411e6d34cabc0cda37MD51TEXTBALDISSERA, MATHEUS DELLAMEA.pdf.txtBALDISSERA, MATHEUS DELLAMEA.pdf.txtExtracted texttext/plain86954http://repositorio.ufsm.br/bitstream/1/9026/2/BALDISSERA%2c%20MATHEUS%20DELLAMEA.pdf.txt1bc69ab53ca5d0f097b148266da7cf0fMD52THUMBNAILBALDISSERA, MATHEUS DELLAMEA.pdf.jpgBALDISSERA, MATHEUS DELLAMEA.pdf.jpgIM Thumbnailimage/jpeg4359http://repositorio.ufsm.br/bitstream/1/9026/3/BALDISSERA%2c%20MATHEUS%20DELLAMEA.pdf.jpgf12b3abd4f89074352bd79103565115fMD531/90262022-03-25 11:46:04.801oai:repositorio.ufsm.br:1/9026Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-03-25T14:46:04Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the activity of three compounds of melaleuca oil against Trypanosoma evansi |
title |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
spellingShingle |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi Baldissera, Matheus Dellaméa Melaleuca alternifolia Surra Plantas medicinais Tripanossomose Melaleuca alternifolia Surra Medicinal plants Tripanosomiasis CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
title_full |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
title_fullStr |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
title_full_unstemmed |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
title_sort |
Avaliação da atividade de três compostos do óleo de melaleuca sobre o Trypanosoma evansi |
author |
Baldissera, Matheus Dellaméa |
author_facet |
Baldissera, Matheus Dellaméa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Monteiro, Silvia Gonzalez |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3762606653182779 |
dc.contributor.referee1.fl_str_mv |
Sangioni, Luis Antonio |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8056805667740451 |
dc.contributor.referee2.fl_str_mv |
Parodi, Thaylise Vey |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4183167775476307 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5978984877357023 |
dc.contributor.author.fl_str_mv |
Baldissera, Matheus Dellaméa |
contributor_str_mv |
Monteiro, Silvia Gonzalez Sangioni, Luis Antonio Parodi, Thaylise Vey |
dc.subject.por.fl_str_mv |
Melaleuca alternifolia Surra Plantas medicinais Tripanossomose |
topic |
Melaleuca alternifolia Surra Plantas medicinais Tripanossomose Melaleuca alternifolia Surra Medicinal plants Tripanosomiasis CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Melaleuca alternifolia Surra Medicinal plants Tripanosomiasis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
This study aimed the evaluation of the trypanocidal activity of terpinen-4-ol, γ-terpinene and α-terpinene against Trypanosoma evansi in vivo and in vitro. The in vitro assay was performed in culture medium of T. evansi, using three different concentrations of each compound (0.5, 1.0 and 2.0%), individually and in synergism. Motile (live) trypomastigotes were counted using a Neubauer chamber after 1, 3, 6 and 9 hours of incubation. Death of parasites occurred in a concentration-dependent manner, except for α-terpinene that was able to eliminate all parasites after 1 hour of incubation at all concentrations tested. Seventy mice were equally assigned into 10 groups (A to J) for the in vivo tests. Group A served as a negative control (healthy animals); groups B - J were infected with 0.1 mL of blood containing 105 T. evansi trypomastigotes. Group B served as a positive control; groups C, D and E received oral doses of 1.0 mL kg-1 of terpinen-4-ol, γ-terpinene and α-terpinene, respectively. Associations of the three compounds were orally administered to groups F I: terpinen-4-ol + γ-terpinene to group F, terpinen-4-ol + α-terpinene to group G, α-terpinene + γ-terpinene at 1.0 mL kg-1 (0.5 mL kg-1 for each compound) to group H, terpinen-4-ol + γ-terpinene + α-terpinene at 1.0 mL kg-1 (0.335 mL kg-1 of each compound) to group I. A therapeutic dose of diminazene aceturate (single dose of 3.5 mg kg-1, intraperitoneally) was administered to Group J aiming test validation. Animals in group E (treated with α-terpinene) showed an increase of longevity when compared to animals in group B. However, no curative effectiveness was observed, since all animals in this group died during the observation period. In order to evaluate the association between α-terpinene and diminazene aceturate, twenty-eight mice were assigned into 4 groups (A, B, C and D, n = 7). Group A included animals uninfected (negative control); group B was composed by infected animals (positive control); group C consisted of infected animals treated with diminazene aceturate (single dose of 3.5 mg kg-1, im); and group D consisted of infected animals treated with an association of diminazene aceturate (single dose of 3.5 mg kg-1, im) and α-terpinene (1.0 ml kg-1, orally administered for 10 days). It was observed, as a result of this experiment, an increase in the curative effectiveness (57.14%) in group D when compared to group C, which showed only 14.28% of curative effectiveness. Based on these results, α-terpinene was the compound present in M. alternifolia essential oil that showed trypanocidal activity. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-05-02 |
dc.date.available.fl_str_mv |
2016-05-02 |
dc.date.issued.fl_str_mv |
2016-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BALDISSERA, Matheus Dellaméa. EVALUATION OF THE ACTIVITY OF THREE COMPOUNDS OF MELALEUCA OIL AGAINST Trypanosoma evansi. 2016. 64 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/9026 |
identifier_str_mv |
BALDISSERA, Matheus Dellaméa. EVALUATION OF THE ACTIVITY OF THREE COMPOUNDS OF MELALEUCA OIL AGAINST Trypanosoma evansi. 2016. 64 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
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http://repositorio.ufsm.br/handle/1/9026 |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Farmacologia |
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UFSM |
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BR |
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Farmacologia |
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Universidade Federal de Santa Maria |
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