Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/14184 |
Resumo: | Recently, it was reported that the Lippia alba essential oil (EO) linalol chemotype causes anesthesia in fish of Rhamdia quelen species. However, it is known that a given vegetal species can present differences in its chemical composition, which varies according to the chemotype, local of sampling, time of year and type of soil. In this way, it is necessary a cautious investigation to determine whether anesthetic and sedative effects of L. alba EO are maintained at different chemotypes, as well as to analyze their effects on fish physiology before recommending it as a safe anesthetic. The present study evaluated the blood, plasmatic and biochemical effects caused from anesthesia with two different chemotypes of L. alba EO: linalol (EO-L) and citral (EO-C) in R. quelen. In the first experiment the concentrations of 100 and 300 μL/L were used to anesthetize the fish. Blood was collected through anesthesia and recovery in order to investigate the ionic, blood and biochemical parameters. The plasma Na+, K+ and Ca2+ levels did not show alterations during anesthesia induction and recovery with both treatments. However, blood glucose increased during anesthesia with both anesthetics. Plasmatic creatinine levels increased at the end of recovery in fish anesthetized with EO-C. In this sense, a second experiment was carried out in order to verify whether the alterations observed in the first experiment could be indicative of tissue damage. Thus, the aim of the second experiment was to investigate the effects of anesthesia on biochemical, antioxidant and tissue damage parameters, replicating the same experimental model used previously. As result, it was observed that although anesthesia with EO-C and EO-l avoided the increase on plasma cortisol levels, EO-C increased thiobarbituric reactive acid substances, as well as protein carbonylation in liver and kidney of fish. On the other hand, EO-L did not cause any type of tissue damage. Thus, this study proves that different chemotypes of a given vegetal species can provoke different physiological effects. In summary, we recommended the use of EO-L as an effective and safe anesthetic to R. quelen. |
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Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelenPhysiological implications of the use of the essential oil of Lippia alba, chemotypes linalool and citral, in anesthesia of Rhamdia quelenCortisolEstresse oxidativoGases sanguíneosÍonsPeixesBlood gasesPlasmaIonCortisolFishCNPQ::CIENCIAS BIOLOGICASRecently, it was reported that the Lippia alba essential oil (EO) linalol chemotype causes anesthesia in fish of Rhamdia quelen species. However, it is known that a given vegetal species can present differences in its chemical composition, which varies according to the chemotype, local of sampling, time of year and type of soil. In this way, it is necessary a cautious investigation to determine whether anesthetic and sedative effects of L. alba EO are maintained at different chemotypes, as well as to analyze their effects on fish physiology before recommending it as a safe anesthetic. The present study evaluated the blood, plasmatic and biochemical effects caused from anesthesia with two different chemotypes of L. alba EO: linalol (EO-L) and citral (EO-C) in R. quelen. In the first experiment the concentrations of 100 and 300 μL/L were used to anesthetize the fish. Blood was collected through anesthesia and recovery in order to investigate the ionic, blood and biochemical parameters. The plasma Na+, K+ and Ca2+ levels did not show alterations during anesthesia induction and recovery with both treatments. However, blood glucose increased during anesthesia with both anesthetics. Plasmatic creatinine levels increased at the end of recovery in fish anesthetized with EO-C. In this sense, a second experiment was carried out in order to verify whether the alterations observed in the first experiment could be indicative of tissue damage. Thus, the aim of the second experiment was to investigate the effects of anesthesia on biochemical, antioxidant and tissue damage parameters, replicating the same experimental model used previously. As result, it was observed that although anesthesia with EO-C and EO-l avoided the increase on plasma cortisol levels, EO-C increased thiobarbituric reactive acid substances, as well as protein carbonylation in liver and kidney of fish. On the other hand, EO-L did not cause any type of tissue damage. Thus, this study proves that different chemotypes of a given vegetal species can provoke different physiological effects. In summary, we recommended the use of EO-L as an effective and safe anesthetic to R. quelen.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESRecentemente, foi reportado que o óleo essencial (OE) de Lippia alba, quimiotipo linalol, causa anestesia em peixes da espécie Rhamdia quelen. No entanto, sabe-se que uma mesma espécie vegetal pode apresentar diferenças em sua composição química, que varia conforme o quimiotipo, local de coleta, época do ano e tipo de solo. Deste modo, faz-se necessário a investigação cautelosa, a fim de averiguar se os efeitos anestésicos e sedativos do OE de L. alba são mantidos frente a diferentes quimiotipos, bem como analisar seus efeitos na fisiologia dos peixes, antes de recomendá-lo como anestésico seguro. O presente estudo avaliou os efeitos sanguíneos, plasmáticos e bioquímicos decorrentes da anestesia com OE de L. alba de dois diferentes quimiotipos: linalol (OE-L) e citral (OE-C) em R. quelen. No primeiro experimento foram utilizadas as concentrações de 100 e 300 μL/L para anestesiar os peixes. Durante a anestesia e recuperação, foi coletado sangue dos animais para averiguar parâmetros iônicos, sanguíneos e bioquímicos. Os níveis plasmáticos de Na+, K+ e Ca2+ não apresentaram nenhuma alteração durante a indução e recuperação anestésica em todos os tratamentos. No entanto, a glicemia aumentou durante a anestesia com ambos anestésicos. Os níveis de creatinina apresentaram um aumento ao final da recuperação anestésica em peixes anestesiados com OE-C. Deste modo, um segundo estudo foi realizado a fim de verificar se as alterações obtidas no primeiro experimento poderiam ser indicativas de algum tipo de dano tecidual. Dessa forma, num segundo experimento, objetivamos averiguar os efeitos desta anestesia, sob parâmetros bioquímicos, antioxidantes e de dano tecidual, repetindo o mesmo desenho experimental anteriormente utilizado. Como resultado, foi observado que embora a anestesia com OE-C e OE-L evite aumento dos níveis de cortisol plasmático, OE-C aumentou os níveis de substâncias reativas ao ácido tiobarbitúrico, bem como a carbonilação das proteínas no fígado e no rim dos peixes. Por outro lado, OE-L não causa nenhum tipo de dano tecidual. Em suma, este estudo comprova que diferentes quimiotipos de uma mesma espécie vegetal podem causar diferentes efeitos fisiológicos. Por fim, recomendamos o uso do OE-L como anestésico eficaz e seguro para R. quelen.Universidade Federal de Santa MariaBrasilCiências BiológicasUFSMPrograma de Pós-Graduação em Biodiversidade AnimalCentro de Ciências Naturais e ExatasBaldisserotto, Bernardohttp://lattes.cnpq.br/1036046601275319Zeppenfeld, Carla Cristinahttp://lattes.cnpq.br/3703608484418673Copatti, Carlos Eduardohttp://lattes.cnpq.br/2254441871778798Cunha, Mauro Alves dahttp://lattes.cnpq.br/9745413680917763Rodrigues, Ricardo Vieirahttp://lattes.cnpq.br/8292167402408126Souza, Carine de Freitas2018-09-04T17:42:18Z2018-09-04T17:42:18Z2018-01-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/14184porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-06-01T15:23:18Zoai:repositorio.ufsm.br:1/14184Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-01T15:23:18Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen Physiological implications of the use of the essential oil of Lippia alba, chemotypes linalool and citral, in anesthesia of Rhamdia quelen |
title |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
spellingShingle |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen Souza, Carine de Freitas Cortisol Estresse oxidativo Gases sanguíneos Íons Peixes Blood gases Plasma Ion Cortisol Fish CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
title_full |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
title_fullStr |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
title_full_unstemmed |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
title_sort |
Implicações fisiológicas do uso do óleo essencial de Lippia alba, quimiotipos linalol e citral, na anestesia de Rhamdia quelen |
author |
Souza, Carine de Freitas |
author_facet |
Souza, Carine de Freitas |
author_role |
author |
dc.contributor.none.fl_str_mv |
Baldisserotto, Bernardo http://lattes.cnpq.br/1036046601275319 Zeppenfeld, Carla Cristina http://lattes.cnpq.br/3703608484418673 Copatti, Carlos Eduardo http://lattes.cnpq.br/2254441871778798 Cunha, Mauro Alves da http://lattes.cnpq.br/9745413680917763 Rodrigues, Ricardo Vieira http://lattes.cnpq.br/8292167402408126 |
dc.contributor.author.fl_str_mv |
Souza, Carine de Freitas |
dc.subject.por.fl_str_mv |
Cortisol Estresse oxidativo Gases sanguíneos Íons Peixes Blood gases Plasma Ion Cortisol Fish CNPQ::CIENCIAS BIOLOGICAS |
topic |
Cortisol Estresse oxidativo Gases sanguíneos Íons Peixes Blood gases Plasma Ion Cortisol Fish CNPQ::CIENCIAS BIOLOGICAS |
description |
Recently, it was reported that the Lippia alba essential oil (EO) linalol chemotype causes anesthesia in fish of Rhamdia quelen species. However, it is known that a given vegetal species can present differences in its chemical composition, which varies according to the chemotype, local of sampling, time of year and type of soil. In this way, it is necessary a cautious investigation to determine whether anesthetic and sedative effects of L. alba EO are maintained at different chemotypes, as well as to analyze their effects on fish physiology before recommending it as a safe anesthetic. The present study evaluated the blood, plasmatic and biochemical effects caused from anesthesia with two different chemotypes of L. alba EO: linalol (EO-L) and citral (EO-C) in R. quelen. In the first experiment the concentrations of 100 and 300 μL/L were used to anesthetize the fish. Blood was collected through anesthesia and recovery in order to investigate the ionic, blood and biochemical parameters. The plasma Na+, K+ and Ca2+ levels did not show alterations during anesthesia induction and recovery with both treatments. However, blood glucose increased during anesthesia with both anesthetics. Plasmatic creatinine levels increased at the end of recovery in fish anesthetized with EO-C. In this sense, a second experiment was carried out in order to verify whether the alterations observed in the first experiment could be indicative of tissue damage. Thus, the aim of the second experiment was to investigate the effects of anesthesia on biochemical, antioxidant and tissue damage parameters, replicating the same experimental model used previously. As result, it was observed that although anesthesia with EO-C and EO-l avoided the increase on plasma cortisol levels, EO-C increased thiobarbituric reactive acid substances, as well as protein carbonylation in liver and kidney of fish. On the other hand, EO-L did not cause any type of tissue damage. Thus, this study proves that different chemotypes of a given vegetal species can provoke different physiological effects. In summary, we recommended the use of EO-L as an effective and safe anesthetic to R. quelen. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-04T17:42:18Z 2018-09-04T17:42:18Z 2018-01-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/14184 |
url |
http://repositorio.ufsm.br/handle/1/14184 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Ciências Biológicas UFSM Programa de Pós-Graduação em Biodiversidade Animal Centro de Ciências Naturais e Exatas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Ciências Biológicas UFSM Programa de Pós-Graduação em Biodiversidade Animal Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922153238888448 |