Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos

Detalhes bibliográficos
Autor(a) principal: Fonseca, Erika Toledo da
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000pcmr
Texto Completo: http://repositorio.ufsm.br/handle/1/10122
Resumo: Rabbits have been used as animal models to study the pathogenesis of neurological infection by bovine herpesvirus type 5 (BHV-5), an important agent of meningoencephalitis in cattle. The olfactory system has been implicated as the main pathway for the virus to reach the brain after replication in the nasal mucosa. To investigate the role of the olfactory route in the pathogenesis of neurological infection, a technique of transfrontal craniotomy for ablation of the main olfactory bulbs (MOBs), using the eyes as the anatomic reference was developed and evaluated. Using this technique, twenty three 30 days-old rabbits were submitted to surgical ablation of the MOBs and subsequently inoculated with BHV-5. After skin, subcutaneous tissue and periosteum incisions, the craniotomy was performed in a point equidistant to the medial corner of the eyes, with a 3 mm drill coupled to a low intensity elyptic drilling machine. The removal of the MOB s tissue was performed by using a number 6 uretral probe coupled to a surgical vaccum pump. The anatomic references used were appropriate in allowing an adequate and easy access to the MOBs. Necropsy performed in three animals after the surgery demonstrated that the surgical procedure was efficient in completely removing the MOB tissue. This was also demonstrated by the interruption of the access of the virus to the brain after intranasal inoculation: only one animal (1/11) in the bulbectomized group developed neurological infection and disease, against 100% (10/10) of the control group. The transfrontal craniotomy using the eyes as the anatomical reference allowed for an adequate access for localization and removal of the MOBs in rabbits. This techique may be used in studies of viral pathogenesis requiring the complete interruption of the olfactory connection to the brain.
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spelling Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicosSurgical ablation of the olfactory bulb in rabbits: a model to study the pathogenisis of neurotropic virus infectionsHerpesvírus bovino tipo 5BHV-5Bulbo olfatórioCirurgiaModelo experimentalCoelhosVaccinesBovine herpesvirus type 1BoHV-1Bovine viral diarrhea virus (BVDV)Experimental modelGuinea pigsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIARabbits have been used as animal models to study the pathogenesis of neurological infection by bovine herpesvirus type 5 (BHV-5), an important agent of meningoencephalitis in cattle. The olfactory system has been implicated as the main pathway for the virus to reach the brain after replication in the nasal mucosa. To investigate the role of the olfactory route in the pathogenesis of neurological infection, a technique of transfrontal craniotomy for ablation of the main olfactory bulbs (MOBs), using the eyes as the anatomic reference was developed and evaluated. Using this technique, twenty three 30 days-old rabbits were submitted to surgical ablation of the MOBs and subsequently inoculated with BHV-5. After skin, subcutaneous tissue and periosteum incisions, the craniotomy was performed in a point equidistant to the medial corner of the eyes, with a 3 mm drill coupled to a low intensity elyptic drilling machine. The removal of the MOB s tissue was performed by using a number 6 uretral probe coupled to a surgical vaccum pump. The anatomic references used were appropriate in allowing an adequate and easy access to the MOBs. Necropsy performed in three animals after the surgery demonstrated that the surgical procedure was efficient in completely removing the MOB tissue. This was also demonstrated by the interruption of the access of the virus to the brain after intranasal inoculation: only one animal (1/11) in the bulbectomized group developed neurological infection and disease, against 100% (10/10) of the control group. The transfrontal craniotomy using the eyes as the anatomical reference allowed for an adequate access for localization and removal of the MOBs in rabbits. This techique may be used in studies of viral pathogenesis requiring the complete interruption of the olfactory connection to the brain.Coelhos têm sido utilizados como modelo para o estudo da neuropatogenia da infecção pelo herpesvírus bovino tipo 5 (BHV-5), um importante agente de doença neurológica em bovinos. O sistema olfatório tem sido apontado como a principal via de acesso do vírus ao cérebro após replicação na cavidade nasal. Para investigar a importância da via olfatória na patogenia da infecção neurológica pelo BHV-5, foi elaborada e avaliada técnica operatória de craniotomia transfrontal para remoção dos bulbos olfatórios (BOs), definindo-se as órbitas como referência anatômica. Foram utilizados 45 coelhos com 30 a 40 dias de idade, sendo 23 submetidos à ablação cirúrgica dos BOs e posteriormente inoculados pela via intranasal (IN) ou no saco conjuntival (IC) com o BHV-5. Após incisões de pele, tecido subcutâneo e periósteo, a craniotomia foi realizada em um ponto eqüidistante entre os cantos mediais dos olhos, com uma broca sulcada de 3mm acoplada a uma perfuratriz elétrica de baixa rotação. A remoção dos BOs foi realizada com uma sonda uretral nº6 acoplada a um aspirador. O estudo macroscópico de três animais após a cirurgia comprovou que o procedimento foi eficiente na remoção total dos BOs. Isso também foi comprovado pela interrupção do acesso do vírus ao córtex cerebral: apenas um animal (1/11 ou 9,1%) no grupo submetido à ablação dos BOs com inoculação IN desenvolveu enfermidade neurológica, contra 100% (10/10) dos coelhos controle. Conclui-se que a técnica de craniotomia transfrontal utilizando a órbita como referência anatômica permite o acesso adequado para localização e remoção dos BOs e pode ser utilizada em estudos de patogenia de infecções por vírus neurotrópicos que exijam a interrupção completa da via olfatória.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaMazzanti, Alexandrehttp://lattes.cnpq.br/3504517995843014Raiser, Alceu Gasparhttp://lattes.cnpq.br/5375404372156000Contesini, Emerson Antoniohttp://lattes.cnpq.br/7232739159397872Fonseca, Erika Toledo da2017-06-022017-06-022006-12-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFONSECA, Erika Toledo da. Surgical ablation of the olfactory bulb in rabbits: a model to study the pathogenisis of neurotropic virus infections. 2006. 31 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/10122ark:/26339/001300000pcmrporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-07-21T18:48:42Zoai:repositorio.ufsm.br:1/10122Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-07-21T18:48:42Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
Surgical ablation of the olfactory bulb in rabbits: a model to study the pathogenisis of neurotropic virus infections
title Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
spellingShingle Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
Fonseca, Erika Toledo da
Herpesvírus bovino tipo 5
BHV-5
Bulbo olfatório
Cirurgia
Modelo experimental
Coelhos
Vaccines
Bovine herpesvirus type 1
BoHV-1
Bovine viral diarrhea virus (BVDV)
Experimental model
Guinea pigs
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
title_full Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
title_fullStr Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
title_full_unstemmed Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
title_sort Ablação cirúrgica dos bulbos olfatórios em coelhos: modelo para estudos de patogenia de infecções por vírus neurotrópicos
author Fonseca, Erika Toledo da
author_facet Fonseca, Erika Toledo da
author_role author
dc.contributor.none.fl_str_mv Mazzanti, Alexandre
http://lattes.cnpq.br/3504517995843014
Raiser, Alceu Gaspar
http://lattes.cnpq.br/5375404372156000
Contesini, Emerson Antonio
http://lattes.cnpq.br/7232739159397872
dc.contributor.author.fl_str_mv Fonseca, Erika Toledo da
dc.subject.por.fl_str_mv Herpesvírus bovino tipo 5
BHV-5
Bulbo olfatório
Cirurgia
Modelo experimental
Coelhos
Vaccines
Bovine herpesvirus type 1
BoHV-1
Bovine viral diarrhea virus (BVDV)
Experimental model
Guinea pigs
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Herpesvírus bovino tipo 5
BHV-5
Bulbo olfatório
Cirurgia
Modelo experimental
Coelhos
Vaccines
Bovine herpesvirus type 1
BoHV-1
Bovine viral diarrhea virus (BVDV)
Experimental model
Guinea pigs
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Rabbits have been used as animal models to study the pathogenesis of neurological infection by bovine herpesvirus type 5 (BHV-5), an important agent of meningoencephalitis in cattle. The olfactory system has been implicated as the main pathway for the virus to reach the brain after replication in the nasal mucosa. To investigate the role of the olfactory route in the pathogenesis of neurological infection, a technique of transfrontal craniotomy for ablation of the main olfactory bulbs (MOBs), using the eyes as the anatomic reference was developed and evaluated. Using this technique, twenty three 30 days-old rabbits were submitted to surgical ablation of the MOBs and subsequently inoculated with BHV-5. After skin, subcutaneous tissue and periosteum incisions, the craniotomy was performed in a point equidistant to the medial corner of the eyes, with a 3 mm drill coupled to a low intensity elyptic drilling machine. The removal of the MOB s tissue was performed by using a number 6 uretral probe coupled to a surgical vaccum pump. The anatomic references used were appropriate in allowing an adequate and easy access to the MOBs. Necropsy performed in three animals after the surgery demonstrated that the surgical procedure was efficient in completely removing the MOB tissue. This was also demonstrated by the interruption of the access of the virus to the brain after intranasal inoculation: only one animal (1/11) in the bulbectomized group developed neurological infection and disease, against 100% (10/10) of the control group. The transfrontal craniotomy using the eyes as the anatomical reference allowed for an adequate access for localization and removal of the MOBs in rabbits. This techique may be used in studies of viral pathogenesis requiring the complete interruption of the olfactory connection to the brain.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-21
2017-06-02
2017-06-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv FONSECA, Erika Toledo da. Surgical ablation of the olfactory bulb in rabbits: a model to study the pathogenisis of neurotropic virus infections. 2006. 31 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2006.
http://repositorio.ufsm.br/handle/1/10122
dc.identifier.dark.fl_str_mv ark:/26339/001300000pcmr
identifier_str_mv FONSECA, Erika Toledo da. Surgical ablation of the olfactory bulb in rabbits: a model to study the pathogenisis of neurotropic virus infections. 2006. 31 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2006.
ark:/26339/001300000pcmr
url http://repositorio.ufsm.br/handle/1/10122
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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