Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18887 |
Resumo: | In this work the ultrasound was used to increase the production of metabolites with antioxidant activity obtained from the submerged fermentation of the fungus Botryosphaeria dothidea, followed by different extraction methods: with soxhlet, extraction with supercritical fluid with CO2 solvent and compressed LPG of the biomass of the microorganism. Ultrasound treatment was applied through a Rotational Central Compound Design (DCCR) with different sonication times (1 to 15 min), at different stages of the fermentation process, under various stirring rates (0 to 150 rpm). Dependent variables to the antioxidant activity of the hydroxyl radical and DPPH (2,2-diphenyl-1-picrylhydrazyl) analyzed in the fermented broth (supernatant). For the hydroxyl radical, although the model did not have a significant adjustment to 5 %, there was an increase in antioxidant activity compared to the control, without sonication, in 70 % of the experiments and with values up to 7 % higher. Rotation was the only significant parameter at 5 % in DPPH analysis. In order to analyze the antioxidant capacity of the biomass resulting from the fermentation processes, extractions were carried out at different temperatures and pressures with the supercritical CO2 (40, 50 and 60 º C and 150, 200 and 250 bar) and compressed LPG (20, 30 and 40 ° C and 15, 25 and 35 bar). The highest yield, 1.14 %, was determined by the CO2 of the supercritical fluid using ethanol as co-solvent, followed by 0.94 % with the solvent LPG and 0.86 % with the solvent CO2. Bioactive compounds with antioxidant activity were found in the extractions with supercritical CO2 and Soxhlet solvent using ethanol and ethyl acetate as solvents, identifying 1,4-diaza-2,5-dioxobicyclo [4.3.0] nanone. |
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2019-11-11T18:45:08Z2019-11-11T18:45:08Z2017-08-17http://repositorio.ufsm.br/handle/1/18887In this work the ultrasound was used to increase the production of metabolites with antioxidant activity obtained from the submerged fermentation of the fungus Botryosphaeria dothidea, followed by different extraction methods: with soxhlet, extraction with supercritical fluid with CO2 solvent and compressed LPG of the biomass of the microorganism. Ultrasound treatment was applied through a Rotational Central Compound Design (DCCR) with different sonication times (1 to 15 min), at different stages of the fermentation process, under various stirring rates (0 to 150 rpm). Dependent variables to the antioxidant activity of the hydroxyl radical and DPPH (2,2-diphenyl-1-picrylhydrazyl) analyzed in the fermented broth (supernatant). For the hydroxyl radical, although the model did not have a significant adjustment to 5 %, there was an increase in antioxidant activity compared to the control, without sonication, in 70 % of the experiments and with values up to 7 % higher. Rotation was the only significant parameter at 5 % in DPPH analysis. In order to analyze the antioxidant capacity of the biomass resulting from the fermentation processes, extractions were carried out at different temperatures and pressures with the supercritical CO2 (40, 50 and 60 º C and 150, 200 and 250 bar) and compressed LPG (20, 30 and 40 ° C and 15, 25 and 35 bar). The highest yield, 1.14 %, was determined by the CO2 of the supercritical fluid using ethanol as co-solvent, followed by 0.94 % with the solvent LPG and 0.86 % with the solvent CO2. Bioactive compounds with antioxidant activity were found in the extractions with supercritical CO2 and Soxhlet solvent using ethanol and ethyl acetate as solvents, identifying 1,4-diaza-2,5-dioxobicyclo [4.3.0] nanone.Neste trabalho o ultrassom foi utilizado para aumentar a produção de metabólitos com atividade antioxidante obtidos a partir da fermentação submersa do fungo Botryosphaeria dothidea, seguida por diferentes métodos de extração: com soxhlet, extração com fluido supercrítico com solvente CO2 e GLP comprimido da biomassa do micro-organismo. O tratamento com o ultrassom foi aplicado através de um Delineamento Composto Central Rotacional (DCCR) com diferentes tempos de sonicação (1 a 15 min), em diferentes fases do processo fermentativo, sob várias velocidades de agitação (0 a 150 rpm), tendo como variáveis dependentes a atividade antioxidante do radical hidroxila e DPPH (2,2-difenil-1-picrilhidrazila) analisados no caldo (sobrenadante) fermentado. Para o radical hidroxila, embora o modelo não tenha tido um ajuste significativo a 5% observou-se um aumento na atividade antioxidante comparado ao controle, sem sonicação, em 70% dos experimentos e com valores até 7% superiores. A rotação foi o único parâmetro significativo a 5% na análise do DPPH. Para análise da capacidade antioxidante da biomassa resultante dos processos fermentativos, foram realizadas extrações, em diferentes temperaturas e pressões com os solventes CO2 do fluido supercrítico (40, 50 e 60 ºC e 150, 200 e 250 bar) e GLP comprimido (20, 30 e 40 ºC e 15, 25 e 35 bar). O maior rendimento, 1,14 %, foi determinado pelo CO2 do fluido supercrítico utilizando etanol como co-solvente, seguido de 0,94 % com o solvente GLP e 0,86 % com o solvente CO2. Compostos bioativos com atividade antioxidante foram encontrados nas extrações com fluido supercrítico solvente CO2 e com soxhlet utilizando etanol e acetato de etila como solventes, identificando o 1,4-diaza-2,5-dioxobicyclo[4.3.0]nanone.porUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia QuímicaUFSMBrasilEngenharia QuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBotryosphaeria dothideaUltrassomExtração supercríticaGLP comprimidoAtividade antioxidanteUltrasoundSupercritical extractionCompressed LPGAntioxidant activityCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAOtimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma PampaMetabolites production optimization with antioxidant activity from the fungus Botryosphaeria dothidea isolate of the Pampa biomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTerra, Lisiane de Marsillachttp://lattes.cnpq.br/3695292052760336Mazutti, Marcio Antoniohttp://lattes.cnpq.br/3520282081196395Mendes, André Aguiarhttp://lattes.cnpq.br/1409264122407992http://lattes.cnpq.br/8944517488740355Valente, Isabela de Lourdes300600000006600abecbeeb-4b09-4f77-94e7-2742b9914f32fee8026d-5e0c-415c-8bd5-8d5d6c22a3af67e239f9-31ad-4d6b-9c76-bea8273292992907cc6b-8007-41ef-9a89-d04255d4688breponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18887/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDIS_PPGEQ_2017_VALENTE_ISABELA.pdfDIS_PPGEQ_2017_VALENTE_ISABELA.pdfDissertação de Mestradoapplication/pdf1304913http://repositorio.ufsm.br/bitstream/1/18887/1/DIS_PPGEQ_2017_VALENTE_ISABELA.pdf62b466a529b61d0673f3672d37fe59acMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-816http://repositorio.ufsm.br/bitstream/1/18887/3/license.txtf8fcb28efb1c8cf0dc096bec902bf4c4MD53TEXTDIS_PPGEQ_2017_VALENTE_ISABELA.pdf.txtDIS_PPGEQ_2017_VALENTE_ISABELA.pdf.txtExtracted texttext/plain148854http://repositorio.ufsm.br/bitstream/1/18887/4/DIS_PPGEQ_2017_VALENTE_ISABELA.pdf.txt410239b91d8276c9d4db119974da21b1MD54THUMBNAILDIS_PPGEQ_2017_VALENTE_ISABELA.pdf.jpgDIS_PPGEQ_2017_VALENTE_ISABELA.pdf.jpgIM Thumbnailimage/jpeg4489http://repositorio.ufsm.br/bitstream/1/18887/5/DIS_PPGEQ_2017_VALENTE_ISABELA.pdf.jpg7c3409cda76f99939a80c026fdf88443MD551/188872022-09-02 10:05:42.103oai:repositorio.ufsm.br:1/18887Q3JlYXRpdmUgQ29tbW9ucw==Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-02T13:05:42Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
dc.title.alternative.eng.fl_str_mv |
Metabolites production optimization with antioxidant activity from the fungus Botryosphaeria dothidea isolate of the Pampa bioma |
title |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
spellingShingle |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa Valente, Isabela de Lourdes Botryosphaeria dothidea Ultrassom Extração supercrítica GLP comprimido Atividade antioxidante Ultrasound Supercritical extraction Compressed LPG Antioxidant activity CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
title_full |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
title_fullStr |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
title_full_unstemmed |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
title_sort |
Otimização da produção e extração de metabólitos com atividade antioxidantes a partir do fungo Botryosphaeria dothidea isolado do bioma Pampa |
author |
Valente, Isabela de Lourdes |
author_facet |
Valente, Isabela de Lourdes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Terra, Lisiane de Marsillac |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3695292052760336 |
dc.contributor.referee1.fl_str_mv |
Mazutti, Marcio Antonio |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3520282081196395 |
dc.contributor.referee2.fl_str_mv |
Mendes, André Aguiar |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1409264122407992 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8944517488740355 |
dc.contributor.author.fl_str_mv |
Valente, Isabela de Lourdes |
contributor_str_mv |
Terra, Lisiane de Marsillac Mazutti, Marcio Antonio Mendes, André Aguiar |
dc.subject.por.fl_str_mv |
Botryosphaeria dothidea Ultrassom Extração supercrítica GLP comprimido Atividade antioxidante |
topic |
Botryosphaeria dothidea Ultrassom Extração supercrítica GLP comprimido Atividade antioxidante Ultrasound Supercritical extraction Compressed LPG Antioxidant activity CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Ultrasound Supercritical extraction Compressed LPG Antioxidant activity |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
In this work the ultrasound was used to increase the production of metabolites with antioxidant activity obtained from the submerged fermentation of the fungus Botryosphaeria dothidea, followed by different extraction methods: with soxhlet, extraction with supercritical fluid with CO2 solvent and compressed LPG of the biomass of the microorganism. Ultrasound treatment was applied through a Rotational Central Compound Design (DCCR) with different sonication times (1 to 15 min), at different stages of the fermentation process, under various stirring rates (0 to 150 rpm). Dependent variables to the antioxidant activity of the hydroxyl radical and DPPH (2,2-diphenyl-1-picrylhydrazyl) analyzed in the fermented broth (supernatant). For the hydroxyl radical, although the model did not have a significant adjustment to 5 %, there was an increase in antioxidant activity compared to the control, without sonication, in 70 % of the experiments and with values up to 7 % higher. Rotation was the only significant parameter at 5 % in DPPH analysis. In order to analyze the antioxidant capacity of the biomass resulting from the fermentation processes, extractions were carried out at different temperatures and pressures with the supercritical CO2 (40, 50 and 60 º C and 150, 200 and 250 bar) and compressed LPG (20, 30 and 40 ° C and 15, 25 and 35 bar). The highest yield, 1.14 %, was determined by the CO2 of the supercritical fluid using ethanol as co-solvent, followed by 0.94 % with the solvent LPG and 0.86 % with the solvent CO2. Bioactive compounds with antioxidant activity were found in the extractions with supercritical CO2 and Soxhlet solvent using ethanol and ethyl acetate as solvents, identifying 1,4-diaza-2,5-dioxobicyclo [4.3.0] nanone. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-08-17 |
dc.date.accessioned.fl_str_mv |
2019-11-11T18:45:08Z |
dc.date.available.fl_str_mv |
2019-11-11T18:45:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18887 |
url |
http://repositorio.ufsm.br/handle/1/18887 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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300600000006 |
dc.relation.confidence.fl_str_mv |
600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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Programa de Pós-Graduação em Engenharia Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Engenharia Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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UFSM |
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