Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6147 |
Resumo: | The persistence of microorganisms in the root canal system after chemical-mechanical preparation plays an important role in the success rate of endodontic treatment. Photodynamic Therapy in endodontics appeared to be a supplement to the disinfection of root canals aiming resolve this persistence. When questioning what the actual antibacterial effect of this treatment in clinical endodontics, we face numerous formulations and concentrations of the photosensitizer used, as well as numerous methodologies to test its antimicrobial activity, offering confusing and inconclusive results. The aim of this study was to evaluate the in vitro antibacterial activity against two strains of Enterococcus faecalis ATCCs 29212 and 51299, of the methylene blue photosensitizer (PS) in different formulations and concentrations cited in the literature. The method used to evaluate the antibacterial activity of the photosensitizer was the microdilution to determine the minimum inhibitory concentration. The 18 test substances, in the literature, were left in contact for five minutes in each well and then followed by the application of the laser (660 nm, Kondortech®, São Carlos, SP, Brazil) for three minutes. The tests were performed according to the Clinical and Laboratory Standards Institute (CLSI 2013). After the incubation period at 35 ± 2 ° C 18-24hs, read visually observing the bacterial growth and is considered where the inhibitory substances were wells without turbidity and / or deposition of sediments at the bottom was carried out. Dissolved in a mixture of 2% chlorhexidine and H₂O₂ (substance 15), and a mixture of glycerol, ethanol, water, hydrogen peroxide and Triton X100 detergent (substance 10) PS inhibited the growth of Enterococcus faecalis resistant microorganism 29212. For the addition of these the mixture PS glycerol, ethanol, and water (substance 13) also showed an inhibitory power. No concentration of methylene blue dissolved in water (300 to 7.5 μmol / L) had able to inhibit the growth of the strains analyzed. Given the literature reviewed and the results of the study to predict the efficacy of PDT against microorganism problem in endodontics, a standardization of the optimal formulation of PS, its contact time and also of important laser parameters is still necessary, as full power , creep and irradiation time. |
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Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontiaEvaluation of antibacterial activity of methylene blue in different formulations used in photodynamictherapyTerapia fotodinâmica antimicrobianaFotossensibilizadorEndodontiaEnterococcus faecalisLaserAntimicrobial photodynamic therapyEndodonticsEnterococcus faecalisLaserMethylene bluePhotosensitizingCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAThe persistence of microorganisms in the root canal system after chemical-mechanical preparation plays an important role in the success rate of endodontic treatment. Photodynamic Therapy in endodontics appeared to be a supplement to the disinfection of root canals aiming resolve this persistence. When questioning what the actual antibacterial effect of this treatment in clinical endodontics, we face numerous formulations and concentrations of the photosensitizer used, as well as numerous methodologies to test its antimicrobial activity, offering confusing and inconclusive results. The aim of this study was to evaluate the in vitro antibacterial activity against two strains of Enterococcus faecalis ATCCs 29212 and 51299, of the methylene blue photosensitizer (PS) in different formulations and concentrations cited in the literature. The method used to evaluate the antibacterial activity of the photosensitizer was the microdilution to determine the minimum inhibitory concentration. The 18 test substances, in the literature, were left in contact for five minutes in each well and then followed by the application of the laser (660 nm, Kondortech®, São Carlos, SP, Brazil) for three minutes. The tests were performed according to the Clinical and Laboratory Standards Institute (CLSI 2013). After the incubation period at 35 ± 2 ° C 18-24hs, read visually observing the bacterial growth and is considered where the inhibitory substances were wells without turbidity and / or deposition of sediments at the bottom was carried out. Dissolved in a mixture of 2% chlorhexidine and H₂O₂ (substance 15), and a mixture of glycerol, ethanol, water, hydrogen peroxide and Triton X100 detergent (substance 10) PS inhibited the growth of Enterococcus faecalis resistant microorganism 29212. For the addition of these the mixture PS glycerol, ethanol, and water (substance 13) also showed an inhibitory power. No concentration of methylene blue dissolved in water (300 to 7.5 μmol / L) had able to inhibit the growth of the strains analyzed. Given the literature reviewed and the results of the study to predict the efficacy of PDT against microorganism problem in endodontics, a standardization of the optimal formulation of PS, its contact time and also of important laser parameters is still necessary, as full power , creep and irradiation time.A permanência dos microrganismos no sistema de canais radiculares após o preparo químico-mecânico desempenha um importante papel na taxa de sucesso do tratamento endodôntico. A Terapia Fotodinâmica surgiu na endodontia para ser um suplemento à desinfecção dos canais radiculares visando dirimir essa persistência. Ao questionarmos qual o real efeito antibacteriano desse tratamento na clínica de endodontia, nos deparamos com inúmeras formulações e concentrações usadas do fotossensibilizador, bem como inúmeras metodologias para testar sua atividade antimicrobiana, oferecendo resultados confusos e não conclusivos. O objetivo deste estudo foi avaliar in vitro a atividade antibacteriana, frente a duas cepas de Enterococcus faecalis ATCCs 29212 e 51299, do agente fotossensibilizador (FS) azul de metileno em diferentes concentrações e formulações citadas na literatura. O método empregado para avaliar a atividade antibacteriana do fotossensibilizador foi a microdiluição em caldo com a determinação da concentração inibitória mínima. As 18 substâncias-teste, citadas na literatura, foram deixadas em contato por cinco minutos em cada poço e então, seguiu-se a aplicação do laser (660nm, Kondortech®, São Carlos, SP, Brazil) por três minutos. Os testes foram realizados de acordo com o Clinical and Laboratory Standards Institute (CLSI 2013). Após o período de incubação de 18-24hs a 35±2ºC, foi realizada a leitura visual observando o crescimento bacteriano, sendo consideradas inibitórias as substâncias onde os poços estavam sem turbidez e/ou depósito de sedimentos no fundo. O FS dissolvido numa mistura de H₂O₂ e clorexidina 2% (substância 15) e numa mistura de glicerol, etanol, água, peróxido de hidrogênio e detergente Triton X100 (substância 10) inibiram o crescimento do Enterococcus faecalis 29212. Para o microrganismo resistente além destas, a mistura do FS em glicerol, etanol e água (substância 13) também apresentou poder inibitório. Nenhuma concentração de azul de metileno dissolvido em água (300 a 7,5 μmol/L) apresentou poder de inibir o crescimento das cepas estudadas. Diante da literatura pesquisada e dos resultados do estudo, para predizer a eficácia da TFD frente ao microrganismo problema na endodontia, ainda é necessário uma padronização da formulação ideal do FS, seu tempo de contato e ainda, de parâmetros importantes do laser, como potência total, fluência e tempo de irradiação.Universidade Federal de Santa MariaBROdontologiaUFSMPrograma de Pós-Graduação em Ciências OdontológicasSchmitz, Marcia da Silvahttp://lattes.cnpq.br/4239733753424639Horner, Rosmarihttp://lattes.cnpq.br/5907084134183708Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770Londero, Aline de Moraes2015-03-242015-03-242014-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfLONDERO, Aline de Moraes. EVALUATION OF ANTIBACTERIAL ACTIVITY OF METHYLENE BLUE IN DIFFERENT FORMULATIONS USED IN PHOTODYNAMICTHERAPY. 2014. 50 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/6147porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-31T13:04:25Zoai:repositorio.ufsm.br:1/6147Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-31T13:04:25Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia Evaluation of antibacterial activity of methylene blue in different formulations used in photodynamictherapy |
title |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
spellingShingle |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia Londero, Aline de Moraes Terapia fotodinâmica antimicrobiana Fotossensibilizador Endodontia Enterococcus faecalis Laser Antimicrobial photodynamic therapy Endodontics Enterococcus faecalis Laser Methylene blue Photosensitizing CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
title_full |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
title_fullStr |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
title_full_unstemmed |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
title_sort |
Avaliação in vitro da atividade antibacteriana do azul de metileno em diferentes formulações utilizados na terapia fotodinâmica na endodontia |
author |
Londero, Aline de Moraes |
author_facet |
Londero, Aline de Moraes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Schmitz, Marcia da Silva http://lattes.cnpq.br/4239733753424639 Horner, Rosmari http://lattes.cnpq.br/5907084134183708 Montagner, Francisco http://lattes.cnpq.br/2937031469516770 |
dc.contributor.author.fl_str_mv |
Londero, Aline de Moraes |
dc.subject.por.fl_str_mv |
Terapia fotodinâmica antimicrobiana Fotossensibilizador Endodontia Enterococcus faecalis Laser Antimicrobial photodynamic therapy Endodontics Enterococcus faecalis Laser Methylene blue Photosensitizing CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
Terapia fotodinâmica antimicrobiana Fotossensibilizador Endodontia Enterococcus faecalis Laser Antimicrobial photodynamic therapy Endodontics Enterococcus faecalis Laser Methylene blue Photosensitizing CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The persistence of microorganisms in the root canal system after chemical-mechanical preparation plays an important role in the success rate of endodontic treatment. Photodynamic Therapy in endodontics appeared to be a supplement to the disinfection of root canals aiming resolve this persistence. When questioning what the actual antibacterial effect of this treatment in clinical endodontics, we face numerous formulations and concentrations of the photosensitizer used, as well as numerous methodologies to test its antimicrobial activity, offering confusing and inconclusive results. The aim of this study was to evaluate the in vitro antibacterial activity against two strains of Enterococcus faecalis ATCCs 29212 and 51299, of the methylene blue photosensitizer (PS) in different formulations and concentrations cited in the literature. The method used to evaluate the antibacterial activity of the photosensitizer was the microdilution to determine the minimum inhibitory concentration. The 18 test substances, in the literature, were left in contact for five minutes in each well and then followed by the application of the laser (660 nm, Kondortech®, São Carlos, SP, Brazil) for three minutes. The tests were performed according to the Clinical and Laboratory Standards Institute (CLSI 2013). After the incubation period at 35 ± 2 ° C 18-24hs, read visually observing the bacterial growth and is considered where the inhibitory substances were wells without turbidity and / or deposition of sediments at the bottom was carried out. Dissolved in a mixture of 2% chlorhexidine and H₂O₂ (substance 15), and a mixture of glycerol, ethanol, water, hydrogen peroxide and Triton X100 detergent (substance 10) PS inhibited the growth of Enterococcus faecalis resistant microorganism 29212. For the addition of these the mixture PS glycerol, ethanol, and water (substance 13) also showed an inhibitory power. No concentration of methylene blue dissolved in water (300 to 7.5 μmol / L) had able to inhibit the growth of the strains analyzed. Given the literature reviewed and the results of the study to predict the efficacy of PDT against microorganism problem in endodontics, a standardization of the optimal formulation of PS, its contact time and also of important laser parameters is still necessary, as full power , creep and irradiation time. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-20 2015-03-24 2015-03-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LONDERO, Aline de Moraes. EVALUATION OF ANTIBACTERIAL ACTIVITY OF METHYLENE BLUE IN DIFFERENT FORMULATIONS USED IN PHOTODYNAMICTHERAPY. 2014. 50 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/6147 |
identifier_str_mv |
LONDERO, Aline de Moraes. EVALUATION OF ANTIBACTERIAL ACTIVITY OF METHYLENE BLUE IN DIFFERENT FORMULATIONS USED IN PHOTODYNAMICTHERAPY. 2014. 50 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/6147 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Odontologia UFSM Programa de Pós-Graduação em Ciências Odontológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Odontologia UFSM Programa de Pós-Graduação em Ciências Odontológicas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922104386781184 |