Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000qk5r |
Texto Completo: | http://repositorio.ufsm.br/handle/1/20615 |
Resumo: | Gyrodactylosis and ichthyophthiriasis resulted in significant economic losses in fish farming. Several products have been used in the treatment of these diseases and few showed satisfactory antiparasitic efficacy. Thus, this work aimed to obtainew antiparasitic drugs from essential oils (EOs) of native medicinal plants: Aloysina gratissima (AEO), Nectandra megapotamica (MEO) e Nectandra grandiflora (GEO). Tchhreo meastsoegnratipahl y ocilosu pwleedr et o ombatasisn esdp ecbtyro mheydtrryo dainsdti lltaot ioflna maen ido niaznaatiolynz edde tebcyt org. aIns vitro assays against Gyrodactylus lilianae were conducted and the concentrations tested were 9 to 90 mg L-1 (AEO) 50 to 500 mg L-1 (MEO) and 10 to 100 mg L-1 (FGroEmO )t,h aei minin vgi ttroo dreetseurlmtsi,n ein thveiv oe ffterecatitvme ecnotn pcreonttorcaotiolsn f(oEr Cg)y orovdear ctthyelo esxisp ohsauvree btimeeen. proposed for each EO, using immersion baths. Juvenile silver catfish (R. quelen) were subjected to two baths with a 24 hour interval between them, at concentrations oafn t9ip0a mrags iLti-c1 faocrt i2v ihty (AagEaOin),s t2 0th0e m togm Lo-1n (tMs EoOf I)c ahnthdy o5p0h mthgir iLu-s1 (mGuElOtif)il,i ibs owtha sfo er v1a hlu. aTtheed in vitro for AEO (9 to 750 mg L -1), while for MEO and GEO the concentrations tested were equivalent to those used against G. lilianae. The treatment protocol against is ichthyophthiriasis consisted of bath lasting five days at a constant concentration of AEO (9 and 15 mg L-1), while for GEO the concentration tested was 15 mg L-1. The main constituents of the AEO were: germacrene D (19.98%), β-caryophyllene (12.97%) and p-cymene (11.88%). However, for MEO α-pinene (25.5%), β-pinene (15.4%) and globulol (10.5%) were the major components, while GEO presented dtheeh ymdaroinf uckoinmopnoen e(1n7ts.0. 8A%ll )t,e sbtiecdyc EloOgse rsmhaocwreedn ea n(1ti1p.a4r7a s%itic) aanctdiv iαty-p aingeanines t( 4G.9. 8li%lia)n aaes and I. multifiliis in in vitro assays. The protocols applied to gyrodactylosis showed efficacy of 98.4% (AEO), 90.7% (MEO) and 95.6% (GEO) after the first bath, while tthhere ea nEthOeslm teinsttiecd e ifnfe vcitvivoe naegsasin swta Gs . 1l0ili0a%na efo, ro anllly EGOEsO a fctaeur stehde mseocrotanldity b oaft hju. vOefn itlhees (8.33% after the second bath). AEO was not effective for treating ichthyophthiriasis, however GEO was able to increase the survival of treated fish. AEO and MEO have potential for development of new commercial antiparasitic drugs for the treatment of gyrodactylosis, while GEO have potential use for the control of ichthyophthiriasis. |
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Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliisEssential oils as antiparasitics against Gyrodactylus lilianae and Ichthyophthirius multifiliisAloysia gratissimaNectandra megapotamicaNectandra grandifloraGyrodactylus lilianaeIchthyophthirius multifiliisRhamdia quelenCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAGyrodactylosis and ichthyophthiriasis resulted in significant economic losses in fish farming. Several products have been used in the treatment of these diseases and few showed satisfactory antiparasitic efficacy. Thus, this work aimed to obtainew antiparasitic drugs from essential oils (EOs) of native medicinal plants: Aloysina gratissima (AEO), Nectandra megapotamica (MEO) e Nectandra grandiflora (GEO). Tchhreo meastsoegnratipahl y ocilosu pwleedr et o ombatasisn esdp ecbtyro mheydtrryo dainsdti lltaot ioflna maen ido niaznaatiolynz edde tebcyt org. aIns vitro assays against Gyrodactylus lilianae were conducted and the concentrations tested were 9 to 90 mg L-1 (AEO) 50 to 500 mg L-1 (MEO) and 10 to 100 mg L-1 (FGroEmO )t,h aei minin vgi ttroo dreetseurlmtsi,n ein thveiv oe ffterecatitvme ecnotn pcreonttorcaotiolsn f(oEr Cg)y orovdear ctthyelo esxisp ohsauvree btimeeen. proposed for each EO, using immersion baths. Juvenile silver catfish (R. quelen) were subjected to two baths with a 24 hour interval between them, at concentrations oafn t9ip0a mrags iLti-c1 faocrt i2v ihty (AagEaOin),s t2 0th0e m togm Lo-1n (tMs EoOf I)c ahnthdy o5p0h mthgir iLu-s1 (mGuElOtif)il,i ibs owtha sfo er v1a hlu. aTtheed in vitro for AEO (9 to 750 mg L -1), while for MEO and GEO the concentrations tested were equivalent to those used against G. lilianae. The treatment protocol against is ichthyophthiriasis consisted of bath lasting five days at a constant concentration of AEO (9 and 15 mg L-1), while for GEO the concentration tested was 15 mg L-1. The main constituents of the AEO were: germacrene D (19.98%), β-caryophyllene (12.97%) and p-cymene (11.88%). However, for MEO α-pinene (25.5%), β-pinene (15.4%) and globulol (10.5%) were the major components, while GEO presented dtheeh ymdaroinf uckoinmopnoen e(1n7ts.0. 8A%ll )t,e sbtiecdyc EloOgse rsmhaocwreedn ea n(1ti1p.a4r7a s%itic) aanctdiv iαty-p aingeanines t( 4G.9. 8li%lia)n aaes and I. multifiliis in in vitro assays. The protocols applied to gyrodactylosis showed efficacy of 98.4% (AEO), 90.7% (MEO) and 95.6% (GEO) after the first bath, while tthhere ea nEthOeslm teinsttiecd e ifnfe vcitvivoe naegsasin swta Gs . 1l0ili0a%na efo, ro anllly EGOEsO a fctaeur stehde mseocrotanldity b oaft hju. vOefn itlhees (8.33% after the second bath). AEO was not effective for treating ichthyophthiriasis, however GEO was able to increase the survival of treated fish. AEO and MEO have potential for development of new commercial antiparasitic drugs for the treatment of gyrodactylosis, while GEO have potential use for the control of ichthyophthiriasis.piscicultura. Vários produtos já foram usados no tratamento destas doenças e poucos apresentaram eficácia antiparasitária satisfatória. Assim, este trabalho visou obter novos produtos antiparasitários a partir de óleos essenciais (OEs) das plantamedicinais nativas: Aloysia gratissima (AEO), Nectandra megapotamica (MEO) es Nectandra grandiflora (GEO). Os OEs foram obtidos por hidrodestilação, analisados pioonri zcarçoãmoa etomg rcahfiaam gaa.s Foosraa mac ocopnladduaz iàd oess tpeesctetrso mine vtritiaro dfere nmtea sàs aGsy reo daaoc tdyelutesc ltioliar npaoer e as concentrações testadas foram de 9 a 90 mg L-1 (AEO), de 50 a 500 mg L-1 (loMnEgOo ) deo 1t0e ma p1o0 0d me ge Lx-p1o (sGiçEãOo). , Ase npdaort idr edteorsm rineasdualta ad ocso nicne nvtirtraoç,ã ofo erafemti vpar o(CpEos) taoos protocolos de tratamentos in vivo para cada OE por banhos de imersão para girodactilose. Os juvenis de jundiá (Rhamdia quelen) foram submetidos a dois banhos de 90 mg por 2 h (AEO) e 1 h com 200 mg L-1 (MEO) e 50 mg L-1 (GEO) sdeon pdaor aos itinat eIcrhvathloy odpeh t2h4ir ihu se nmtruelt iefilleiiss . foAi aatvivaildiaaddae iann vtiiptraor apsairtáar iAaE fOre n(9te a a o7s5 0to mmgo nLt-e1)s, eànquqeualanst ou sqaudea,s pcaornat raM GEO. l ileia nGaEeO. O, apsro ctoocnocleon dtrea çtrõaetasm teensttaod daas icfotiroafmtir íaesqeu icvoanlesnistteius em banhos com duração de cinco dias a uma concentração constante de AEO (9 e 1co5n mstgit uLin-1t)e, se ndqou aAnEtoO qfuoera pma:r ag eGrmEOac are cnoon cDe n(1tr9a,ç9ã8o% f)o, i βd-ec a1r5io mfilegn Lo- 1.( 1O2s,9 p7r%in)c iep apiscimeno (11,88%). Já para MEO os componentes majoritários foram α-pineno (25,5%), β-pineno (15,4%) e globulol (10,5%), enquanto que GEO apresentou dehidrofuquinona (17.08%), biciclogermacreno (11.47%) e α-pineno (4.98%) como parnintipciapraaissi tárciao ncsotintutrian teGs.. liliTaondaoes e Io. sm uOltiEfilsii s nteosst aednossa ioas pirne sveitnrtoa.r aOms praotitvoicdoaldoes aplicados para girodactilose apresentaram eficácia de 98,4% (AEO), 90,7% (MEO) e 9ef5ic,6á%ci a (GanEtOi-h) elampíónsti coa fporii mdeei r1o0 0b%an hpoa,r ae ntoqduoasn toos qOuEes .a pDóoss otr êsse OguEnsd ote sbtaadnohso ina vivo frente a G. lilianae, apenas GEO provocou mortalidade dos juvenis (8,33% após o segundo banho). O AEO não demonstrou ser eficaz para o tratamento da ictiofitíriase, já GEO foi capaz de aumentar a sobrevivência dos peixes tratados. Os AEO e MEO apresentam potencial para o desenvolvimento de novos antiparasitários comerciais para o tratamento da girodactilose, enquanto GEO tem potencial utilização para o controle da ictioftiríase.Universidade Federal de Santa MariaBrasilFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaCentro de Ciências da SaúdeHeinzmann, Berta Mariahttp://lattes.cnpq.br/0786124562427815Baldisserotto, BernardoPereira Junior, JoaberSilva, Lenise de LimaGarcia, Luciano de OliveiraCunha, Mauro Alves daMurari, Anelise Levay2021-04-16T22:33:02Z2021-04-16T22:33:02Z2016-03-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/20615ark:/26339/001300000qk5rporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-04-17T06:03:22Zoai:repositorio.ufsm.br:1/20615Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-04-17T06:03:22Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis Essential oils as antiparasitics against Gyrodactylus lilianae and Ichthyophthirius multifiliis |
title |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
spellingShingle |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis Murari, Anelise Levay Aloysia gratissima Nectandra megapotamica Nectandra grandiflora Gyrodactylus lilianae Ichthyophthirius multifiliis Rhamdia quelen CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
title_full |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
title_fullStr |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
title_full_unstemmed |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
title_sort |
Óleos essenciais como antiparasitários contra Gyrodactylus lilianae e Ichthyophthirius multifiliis |
author |
Murari, Anelise Levay |
author_facet |
Murari, Anelise Levay |
author_role |
author |
dc.contributor.none.fl_str_mv |
Heinzmann, Berta Maria http://lattes.cnpq.br/0786124562427815 Baldisserotto, Bernardo Pereira Junior, Joaber Silva, Lenise de Lima Garcia, Luciano de Oliveira Cunha, Mauro Alves da |
dc.contributor.author.fl_str_mv |
Murari, Anelise Levay |
dc.subject.por.fl_str_mv |
Aloysia gratissima Nectandra megapotamica Nectandra grandiflora Gyrodactylus lilianae Ichthyophthirius multifiliis Rhamdia quelen CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Aloysia gratissima Nectandra megapotamica Nectandra grandiflora Gyrodactylus lilianae Ichthyophthirius multifiliis Rhamdia quelen CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Gyrodactylosis and ichthyophthiriasis resulted in significant economic losses in fish farming. Several products have been used in the treatment of these diseases and few showed satisfactory antiparasitic efficacy. Thus, this work aimed to obtainew antiparasitic drugs from essential oils (EOs) of native medicinal plants: Aloysina gratissima (AEO), Nectandra megapotamica (MEO) e Nectandra grandiflora (GEO). Tchhreo meastsoegnratipahl y ocilosu pwleedr et o ombatasisn esdp ecbtyro mheydtrryo dainsdti lltaot ioflna maen ido niaznaatiolynz edde tebcyt org. aIns vitro assays against Gyrodactylus lilianae were conducted and the concentrations tested were 9 to 90 mg L-1 (AEO) 50 to 500 mg L-1 (MEO) and 10 to 100 mg L-1 (FGroEmO )t,h aei minin vgi ttroo dreetseurlmtsi,n ein thveiv oe ffterecatitvme ecnotn pcreonttorcaotiolsn f(oEr Cg)y orovdear ctthyelo esxisp ohsauvree btimeeen. proposed for each EO, using immersion baths. Juvenile silver catfish (R. quelen) were subjected to two baths with a 24 hour interval between them, at concentrations oafn t9ip0a mrags iLti-c1 faocrt i2v ihty (AagEaOin),s t2 0th0e m togm Lo-1n (tMs EoOf I)c ahnthdy o5p0h mthgir iLu-s1 (mGuElOtif)il,i ibs owtha sfo er v1a hlu. aTtheed in vitro for AEO (9 to 750 mg L -1), while for MEO and GEO the concentrations tested were equivalent to those used against G. lilianae. The treatment protocol against is ichthyophthiriasis consisted of bath lasting five days at a constant concentration of AEO (9 and 15 mg L-1), while for GEO the concentration tested was 15 mg L-1. The main constituents of the AEO were: germacrene D (19.98%), β-caryophyllene (12.97%) and p-cymene (11.88%). However, for MEO α-pinene (25.5%), β-pinene (15.4%) and globulol (10.5%) were the major components, while GEO presented dtheeh ymdaroinf uckoinmopnoen e(1n7ts.0. 8A%ll )t,e sbtiecdyc EloOgse rsmhaocwreedn ea n(1ti1p.a4r7a s%itic) aanctdiv iαty-p aingeanines t( 4G.9. 8li%lia)n aaes and I. multifiliis in in vitro assays. The protocols applied to gyrodactylosis showed efficacy of 98.4% (AEO), 90.7% (MEO) and 95.6% (GEO) after the first bath, while tthhere ea nEthOeslm teinsttiecd e ifnfe vcitvivoe naegsasin swta Gs . 1l0ili0a%na efo, ro anllly EGOEsO a fctaeur stehde mseocrotanldity b oaft hju. vOefn itlhees (8.33% after the second bath). AEO was not effective for treating ichthyophthiriasis, however GEO was able to increase the survival of treated fish. AEO and MEO have potential for development of new commercial antiparasitic drugs for the treatment of gyrodactylosis, while GEO have potential use for the control of ichthyophthiriasis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-18 2021-04-16T22:33:02Z 2021-04-16T22:33:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/20615 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000qk5r |
url |
http://repositorio.ufsm.br/handle/1/20615 |
identifier_str_mv |
ark:/26339/001300000qk5r |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Farmacologia Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Farmacologia Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1821326021293506560 |