Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/26837 |
Resumo: | Two experiments were designed to evaluate the effect of a simulated transport of oocytes to be used in bovine embryo production. Cumulus-oocytes complex -COCs - (n=1586) obtained from slaughterhouse ovaries were submitted to IVM in medium TCM+HEPES for differents periods. In experiment I (7 repetitions) IVM of oocytes in TCM-199 performed in incubator with 5% CO2 in air during 24h (Control Group, n=208) was compared with IVM in TCM+HEPES in a 39oC water bath during 6h followed by complementar IVM for 18h under the same conditions of Control Group. In experiment II (11 repetitions), oocytes were randomly distributed in four treatments. In the Control Group, oocytes (n=296) were matured for 24h in modified TCM-199 plus Bicarbonate, HEPES, rFSH-h, Estrus Cow Serum (ECS), and Piruvate at 39oC in incubator with 5% CO2 and saturated humidity. Oocytes exposed to a simulated transport remained for 6 (T6, n=286), 12 (T12, n=294) and 18h (T18, n=301) in maturation medium containing TCM + HEPES, in a 39oC water bath, with the same components used in the Control Group, except for bicarbonate. After completion of each transport period, oocytes were transferred to plates with maturation medium to reach 24h in incubator. In Experiments I and II, after maturation, oocytes were fertilized in FERT-TALP with heparin using spermatozoa (1 x 106/mL) sorted by Swim-Up. Thereafter, possible zygotes were cultured in SOF for 8 days. In Experiment I, cleavage and blastocyst rates in D7 and D9 were similar. In experiment II, embryonic development rates at D7 were similar for Control, T6 and T12 groups, with a reduction (P<0,05) observed for group T18 when compared with Control and T12 groups. At D9, there was a lower production of expanded plus hatched blastocysts in T18 (P<0,05), without difference in hatched rate. The average number of cells from hatched blastocysts was similar in Control (136), T6 (125,5) and T12 (126,8) groups. These results indicate the possibility of transporting bovine oocytes in maturation medium containing TCM + HEPES, with no controlled gaseous atmosphere environment, at 39oC, for up to 12h. This technique offers a practical and efficient alternative for the transport of bovine oocytes for IVP. |
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Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosaTransport of bovine oocytes in maturation medium without a controlled gaseous atmosphereBovinosOócitoPIVOPUTransporteBovineOocytesIVPTransportCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIATwo experiments were designed to evaluate the effect of a simulated transport of oocytes to be used in bovine embryo production. Cumulus-oocytes complex -COCs - (n=1586) obtained from slaughterhouse ovaries were submitted to IVM in medium TCM+HEPES for differents periods. In experiment I (7 repetitions) IVM of oocytes in TCM-199 performed in incubator with 5% CO2 in air during 24h (Control Group, n=208) was compared with IVM in TCM+HEPES in a 39oC water bath during 6h followed by complementar IVM for 18h under the same conditions of Control Group. In experiment II (11 repetitions), oocytes were randomly distributed in four treatments. In the Control Group, oocytes (n=296) were matured for 24h in modified TCM-199 plus Bicarbonate, HEPES, rFSH-h, Estrus Cow Serum (ECS), and Piruvate at 39oC in incubator with 5% CO2 and saturated humidity. Oocytes exposed to a simulated transport remained for 6 (T6, n=286), 12 (T12, n=294) and 18h (T18, n=301) in maturation medium containing TCM + HEPES, in a 39oC water bath, with the same components used in the Control Group, except for bicarbonate. After completion of each transport period, oocytes were transferred to plates with maturation medium to reach 24h in incubator. In Experiments I and II, after maturation, oocytes were fertilized in FERT-TALP with heparin using spermatozoa (1 x 106/mL) sorted by Swim-Up. Thereafter, possible zygotes were cultured in SOF for 8 days. In Experiment I, cleavage and blastocyst rates in D7 and D9 were similar. In experiment II, embryonic development rates at D7 were similar for Control, T6 and T12 groups, with a reduction (P<0,05) observed for group T18 when compared with Control and T12 groups. At D9, there was a lower production of expanded plus hatched blastocysts in T18 (P<0,05), without difference in hatched rate. The average number of cells from hatched blastocysts was similar in Control (136), T6 (125,5) and T12 (126,8) groups. These results indicate the possibility of transporting bovine oocytes in maturation medium containing TCM + HEPES, with no controlled gaseous atmosphere environment, at 39oC, for up to 12h. This technique offers a practical and efficient alternative for the transport of bovine oocytes for IVP.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESDois experimentos foram delineados para avaliar o efeito do transporte simulado de oócitos para a produção de embriões bovinos. Complexos cumulus-oócitos (CCO’s, n=1586) obtidos de ovários de matadouro foram submetidos ao transporte simulado em meio de MIV TCM+HEPES por diferentes períodos. No Experimento I (7 repetições), a MIV dos oócitos, efetuada em TCM 199, em estufa com 5% de CO2 em ar, durante 24h (grupo Controle, n=208), foi comparada à MIV em TCM+HEPES, em banho-maria a 39ºC, durante 6h, seguida de MIV complementar por 18h, como no grupo Controle. No experimento II (11 repetições), os oócitos foram distribuídos aleatoriamente em 4 tratamentos. No grupo Controle, os oócitos (n=296) foram maturados por 24h em TCM-199 modificado, acrescido de HEPES, rFHS-h, Soro de Vaca em Estro em Estro (SVE) e piruvato, em estufa a 39ºC, com 5% de CO2 em ar e umidade saturada. Os oócitos submetidos ao transporte simulado, permaneceram por 6 (T6, n=286), 12 (T12, n=294) e 18h (T18, n=301) em meio de MIV TCM+HEPES, em banho-maria a 39ºC, com os mesmos componentes usados no grupo Controle, exceto o bicarbonato. Encerrado cada um dos períodos de transporte, os oócitos foram transferidos para placas com meio de MIV até completarem 24h em estufa. Nos experimentos I e II, após a maturação, os oócitos foram fecundados em FERT-TALP com heparina e espermatozóides (1 x 106/mL) selecionados por migração ascendente. Os possíveis zigotos foram cultivados em SOF por 8 dias. No experimento I, as taxas de clivagem e de blastocistos em D7 e D9 foram similares. No experimento II, as taxas de desenvolvimento embrionário em D7 foram semelhantes para o grupo controle, T6 e T12, com uma redução observada no grupo T18 quando comparada aos grupo Controle e T12 (P<0,05). Em D9, houve uma produção mais baixa de blastocistos expandidos mais eclodidos no grupo T18 (P<0,05), mas não houve diferença na taxa de eclosão. O numero médio de células dos blastocistos eclodidos foi semelhante nos grupos Controle (136), T6 (125,5) e T12 (126,8). Esses resultados indicam a possibilidade de transportar oócitos bovinos em meio de MIV TCM+HEPES, sem controle de atmosfera gasosa, a 39ºC, por até 12h. Esta técnica oferece uma alternativa prática e eficiente para o transporte de oócitos bovinos para a PIV.Universidade Federal de Santa MariaBrasilMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaCentro de Ciências RuraisSilva, Carlos Antonio Mondinohttp://lattes.cnpq.br/8811861305924545Rubin, Mara Iolanda BatistellaMezzalira, AlceuLeivas, Fábio Gallas2022-11-10T22:03:19Z2022-11-10T22:03:19Z2002-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/26837porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-11-10T22:03:19Zoai:repositorio.ufsm.br:1/26837Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-11-10T22:03:19Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa Transport of bovine oocytes in maturation medium without a controlled gaseous atmosphere |
title |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
spellingShingle |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa Leivas, Fábio Gallas Bovinos Oócito PIV OPU Transporte Bovine Oocytes IVP Transport CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
title_full |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
title_fullStr |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
title_full_unstemmed |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
title_sort |
Transporte de oócitos bovinos em meio de maturação sem controle de atmosfera gasosa |
author |
Leivas, Fábio Gallas |
author_facet |
Leivas, Fábio Gallas |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Carlos Antonio Mondino http://lattes.cnpq.br/8811861305924545 Rubin, Mara Iolanda Batistella Mezzalira, Alceu |
dc.contributor.author.fl_str_mv |
Leivas, Fábio Gallas |
dc.subject.por.fl_str_mv |
Bovinos Oócito PIV OPU Transporte Bovine Oocytes IVP Transport CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Bovinos Oócito PIV OPU Transporte Bovine Oocytes IVP Transport CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Two experiments were designed to evaluate the effect of a simulated transport of oocytes to be used in bovine embryo production. Cumulus-oocytes complex -COCs - (n=1586) obtained from slaughterhouse ovaries were submitted to IVM in medium TCM+HEPES for differents periods. In experiment I (7 repetitions) IVM of oocytes in TCM-199 performed in incubator with 5% CO2 in air during 24h (Control Group, n=208) was compared with IVM in TCM+HEPES in a 39oC water bath during 6h followed by complementar IVM for 18h under the same conditions of Control Group. In experiment II (11 repetitions), oocytes were randomly distributed in four treatments. In the Control Group, oocytes (n=296) were matured for 24h in modified TCM-199 plus Bicarbonate, HEPES, rFSH-h, Estrus Cow Serum (ECS), and Piruvate at 39oC in incubator with 5% CO2 and saturated humidity. Oocytes exposed to a simulated transport remained for 6 (T6, n=286), 12 (T12, n=294) and 18h (T18, n=301) in maturation medium containing TCM + HEPES, in a 39oC water bath, with the same components used in the Control Group, except for bicarbonate. After completion of each transport period, oocytes were transferred to plates with maturation medium to reach 24h in incubator. In Experiments I and II, after maturation, oocytes were fertilized in FERT-TALP with heparin using spermatozoa (1 x 106/mL) sorted by Swim-Up. Thereafter, possible zygotes were cultured in SOF for 8 days. In Experiment I, cleavage and blastocyst rates in D7 and D9 were similar. In experiment II, embryonic development rates at D7 were similar for Control, T6 and T12 groups, with a reduction (P<0,05) observed for group T18 when compared with Control and T12 groups. At D9, there was a lower production of expanded plus hatched blastocysts in T18 (P<0,05), without difference in hatched rate. The average number of cells from hatched blastocysts was similar in Control (136), T6 (125,5) and T12 (126,8) groups. These results indicate the possibility of transporting bovine oocytes in maturation medium containing TCM + HEPES, with no controlled gaseous atmosphere environment, at 39oC, for up to 12h. This technique offers a practical and efficient alternative for the transport of bovine oocytes for IVP. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-02-01 2022-11-10T22:03:19Z 2022-11-10T22:03:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26837 |
url |
http://repositorio.ufsm.br/handle/1/26837 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922054148456448 |