O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6728 |
Resumo: | By definition Traumatic brain injury (TBI) is a common involvement in all societies and covers the entire set of processes that alone or in combination can damage the brain. In children and adolescents TBI is an interruption in their normal development, with estimates ranging from 200 to 500 cases per 100,000 per year. Most cases are characterized as mild, with few long-term consequences, however, a significant portion of young people suffer more serious injuries. Furthermore, Juvenile TBI is the major cause of death and disability in children and adolescents. An important factor is that the sports and youth have always been closely related. In this sense, it called attention traumas, especially for young people during practice of contact sports such as martial arts, football, ice hockey, baseball. Because of the wide variety of conditions associated with TBI, there is considerable interest in the development and subsequent application of biochemical markers that relate the severity of brain damage with the development of neurological problems such as memory and learning deficits. In this context, this study aimed, at first, to see if the young animals subjected to TBI had cognitive impairment fifteen days after the injury and whether creatine supplementation has protective effect by changing the activity of CK enzyme, modulating the expression of AMPK protein, CREB, p-CREB and BDNF involved in cognitive impairment and histological damage generated by TBI To this end the present study used young male Wistar rats at 35 days of life subjected to TBI or subjected to all processes except TBI were divided into four groups which were randomized to receive the Cr supplementation (300 mg / kg po) was suspended in 0.5% CMC or vehicle (CMC) twice daily for a period of 14 days. it was shown that animals on submitted to TBI showed a reduction in cognitive functions evaluated by 15 days object recognition task after TBI. The animals that received creatine supplementation did not have their compromised functions. Our biochemical data revealed that the activity of the enzyme creatine kinase was increased fifteen days after trauma, in the same period the TBI did not alter the expression of AMPK however creatine supplementation increased its expression, suggesting a connection between CK and AMPK after TBI, since the creatine supplementation was effective in raising the activity of CK while increased expression of AMPK also caused a significant increase in the ratio of CREB and p-CREB in animals that were supplemented. We also note the participation of BDNF, whose expression this increases the animals submitted to the TBI and were supplemented with creatine, protection evidenced by creatine in lesion volume induced by the TBI. |
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O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovensThe effect of creatine supplementation on cognitive deficit induced by traumatic brain injury in juvenile ratsTraumatismo cranioencefálico por percussão de fluidoRatos jovensMemória de reconhecimentoDéficit cognitivoSuplementação com creatinaTraumatic brain injury for percussion fluid (FPI)Juvenile ratsRecognition memoryCognitive deficitCreatine supplementationCNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICABy definition Traumatic brain injury (TBI) is a common involvement in all societies and covers the entire set of processes that alone or in combination can damage the brain. In children and adolescents TBI is an interruption in their normal development, with estimates ranging from 200 to 500 cases per 100,000 per year. Most cases are characterized as mild, with few long-term consequences, however, a significant portion of young people suffer more serious injuries. Furthermore, Juvenile TBI is the major cause of death and disability in children and adolescents. An important factor is that the sports and youth have always been closely related. In this sense, it called attention traumas, especially for young people during practice of contact sports such as martial arts, football, ice hockey, baseball. Because of the wide variety of conditions associated with TBI, there is considerable interest in the development and subsequent application of biochemical markers that relate the severity of brain damage with the development of neurological problems such as memory and learning deficits. In this context, this study aimed, at first, to see if the young animals subjected to TBI had cognitive impairment fifteen days after the injury and whether creatine supplementation has protective effect by changing the activity of CK enzyme, modulating the expression of AMPK protein, CREB, p-CREB and BDNF involved in cognitive impairment and histological damage generated by TBI To this end the present study used young male Wistar rats at 35 days of life subjected to TBI or subjected to all processes except TBI were divided into four groups which were randomized to receive the Cr supplementation (300 mg / kg po) was suspended in 0.5% CMC or vehicle (CMC) twice daily for a period of 14 days. it was shown that animals on submitted to TBI showed a reduction in cognitive functions evaluated by 15 days object recognition task after TBI. The animals that received creatine supplementation did not have their compromised functions. Our biochemical data revealed that the activity of the enzyme creatine kinase was increased fifteen days after trauma, in the same period the TBI did not alter the expression of AMPK however creatine supplementation increased its expression, suggesting a connection between CK and AMPK after TBI, since the creatine supplementation was effective in raising the activity of CK while increased expression of AMPK also caused a significant increase in the ratio of CREB and p-CREB in animals that were supplemented. We also note the participation of BDNF, whose expression this increases the animals submitted to the TBI and were supplemented with creatine, protection evidenced by creatine in lesion volume induced by the TBI.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorPor definição o Traumatismo Cranioencefálico (TCE) é um acometimento comum em todas as sociedades e abrange todo o conjunto de processos que sozinhos ou em combinação podem danificar o encéfalo. Em crianças e adolescentes o TCE representa uma interrupção em seu desenvolvimento normal, com estimativas que variam de 200 a 500 casos para cada 100.000 ao ano. A maioria dos casos é caracterizada como leve, com poucas consequências a longo prazo, entretanto, uma porção significativa de jovens sofrerá ferimentos mais graves. Além disso, o TCE juvenil é a principal causa de morte e incapacidade em crianças e adolescente. Neste sentido, chama-se a atenção traumas sofridos, principalmente por jovens durante pratica de esportes de contato como as artes marciais, o futebol americano, hóquei no gelo, baseball. Devido a grande variedade de condições associadas ao TCE, há um considerável interesse no desenvolvimento e posterior aplicação de marcadores bioquímicos que relacionem a gravidade do dano cerebral com o desenvolvimento de problemas neurológicos, como déficits de memória e aprendizado. Neste contexto, o presente trabalho teve como objetivo, verificar se o se os animais jovens submetidos ao TCE apresentavam déficit cognitivo quinze dias após a lesão e se a suplementação com creatina possui efeito protetor, alterando a atividade da enzima CK, modulando a expressão das proteínas AMPK, CREB, p-CREB e BDNF envolvidas no déficit cognitivo e no dano histológico gerado pelo TCE. Para tal o presente estudo utilizou ratos Wistar jovens machos aos 35 dia de vida submetidos ao TCE ou submetido a todos os processos exceto o TCE, foram divididos em quatro grupos onde foram aleatoriamente separados, para receber a suplementação com Cr (300 mg / kg, p.o), suspensa em CMC a 0,5% ou veículo (CMC) duas vezes ao dia por um período de 14 dias. Foi evidenciado que os animais submetidos ao TCE apresentaram uma redução nas funções cognitivas avaliadas pela tarefa de reconhecimento de objeto 15 dias após o TCE. Já os animais que receberam a suplementação com creatina não tiveram suas funções comprometidas. Nossos dados bioquímicos revelaram que a atividade da enzima creatina quinase estava aumentada quinze dias pós-trauma, no mesmo período o TCE não alterou a expressão de AMPK porem a suplementação com creatina aumentou sua expressão, sugerindo uma conexão entre CK e AMPK após o TCE, uma vez que a suplementação com creatina foi efetiva em elevar a atividade da CK ao mesmo tempo que elevou a expressão de AMPK, também causou um aumento significativo na razão entre CREB e p-CREB nos animais que foram suplementados. Observamos também a participação do BDNF, cuja expressão esta aumenta nos animais que foram submetidos ao TCE e foram suplementados com creatina, na proteção evidenciada pela creatina no volume da lesão induzida pelo TCE.Universidade Federal de Santa MariaBREducação FísicaUFSMPrograma de Pós-Graduação em Educação FísicaRoyes, Luiz Fernando Freirehttp://lattes.cnpq.br/0543081555633400Souza, Mauren Assis dehttp://lattes.cnpq.br/1571752069730472Brescian, Guilherme Bergamaschihttp://lattes.cnpq.br/1247251997170368Busanello, Guilherme Lago2016-02-152016-02-152015-07-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBUSANELLO, Guilherme Lago. The effect of creatine supplementation on cognitive deficit induced by traumatic brain injury in juvenile rats. 2015. 84 f. Dissertação (Mestrado em Educação Física) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/6728porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-31T17:03:56Zoai:repositorio.ufsm.br:1/6728Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-31T17:03:56Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens The effect of creatine supplementation on cognitive deficit induced by traumatic brain injury in juvenile rats |
title |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
spellingShingle |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens Busanello, Guilherme Lago Traumatismo cranioencefálico por percussão de fluido Ratos jovens Memória de reconhecimento Déficit cognitivo Suplementação com creatina Traumatic brain injury for percussion fluid (FPI) Juvenile rats Recognition memory Cognitive deficit Creatine supplementation CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA |
title_short |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
title_full |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
title_fullStr |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
title_full_unstemmed |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
title_sort |
O efeito da suplementação com creatina sobre o deficit cognitivo induzido pelo traumatismo cranioencefálico em ratos jovens |
author |
Busanello, Guilherme Lago |
author_facet |
Busanello, Guilherme Lago |
author_role |
author |
dc.contributor.none.fl_str_mv |
Royes, Luiz Fernando Freire http://lattes.cnpq.br/0543081555633400 Souza, Mauren Assis de http://lattes.cnpq.br/1571752069730472 Brescian, Guilherme Bergamaschi http://lattes.cnpq.br/1247251997170368 |
dc.contributor.author.fl_str_mv |
Busanello, Guilherme Lago |
dc.subject.por.fl_str_mv |
Traumatismo cranioencefálico por percussão de fluido Ratos jovens Memória de reconhecimento Déficit cognitivo Suplementação com creatina Traumatic brain injury for percussion fluid (FPI) Juvenile rats Recognition memory Cognitive deficit Creatine supplementation CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA |
topic |
Traumatismo cranioencefálico por percussão de fluido Ratos jovens Memória de reconhecimento Déficit cognitivo Suplementação com creatina Traumatic brain injury for percussion fluid (FPI) Juvenile rats Recognition memory Cognitive deficit Creatine supplementation CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA |
description |
By definition Traumatic brain injury (TBI) is a common involvement in all societies and covers the entire set of processes that alone or in combination can damage the brain. In children and adolescents TBI is an interruption in their normal development, with estimates ranging from 200 to 500 cases per 100,000 per year. Most cases are characterized as mild, with few long-term consequences, however, a significant portion of young people suffer more serious injuries. Furthermore, Juvenile TBI is the major cause of death and disability in children and adolescents. An important factor is that the sports and youth have always been closely related. In this sense, it called attention traumas, especially for young people during practice of contact sports such as martial arts, football, ice hockey, baseball. Because of the wide variety of conditions associated with TBI, there is considerable interest in the development and subsequent application of biochemical markers that relate the severity of brain damage with the development of neurological problems such as memory and learning deficits. In this context, this study aimed, at first, to see if the young animals subjected to TBI had cognitive impairment fifteen days after the injury and whether creatine supplementation has protective effect by changing the activity of CK enzyme, modulating the expression of AMPK protein, CREB, p-CREB and BDNF involved in cognitive impairment and histological damage generated by TBI To this end the present study used young male Wistar rats at 35 days of life subjected to TBI or subjected to all processes except TBI were divided into four groups which were randomized to receive the Cr supplementation (300 mg / kg po) was suspended in 0.5% CMC or vehicle (CMC) twice daily for a period of 14 days. it was shown that animals on submitted to TBI showed a reduction in cognitive functions evaluated by 15 days object recognition task after TBI. The animals that received creatine supplementation did not have their compromised functions. Our biochemical data revealed that the activity of the enzyme creatine kinase was increased fifteen days after trauma, in the same period the TBI did not alter the expression of AMPK however creatine supplementation increased its expression, suggesting a connection between CK and AMPK after TBI, since the creatine supplementation was effective in raising the activity of CK while increased expression of AMPK also caused a significant increase in the ratio of CREB and p-CREB in animals that were supplemented. We also note the participation of BDNF, whose expression this increases the animals submitted to the TBI and were supplemented with creatine, protection evidenced by creatine in lesion volume induced by the TBI. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-03 2016-02-15 2016-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BUSANELLO, Guilherme Lago. The effect of creatine supplementation on cognitive deficit induced by traumatic brain injury in juvenile rats. 2015. 84 f. Dissertação (Mestrado em Educação Física) - Universidade Federal de Santa Maria, Santa Maria, 2015. http://repositorio.ufsm.br/handle/1/6728 |
identifier_str_mv |
BUSANELLO, Guilherme Lago. The effect of creatine supplementation on cognitive deficit induced by traumatic brain injury in juvenile rats. 2015. 84 f. Dissertação (Mestrado em Educação Física) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/6728 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Educação Física UFSM Programa de Pós-Graduação em Educação Física |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Educação Física UFSM Programa de Pós-Graduação em Educação Física |
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reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
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Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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