Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores

Detalhes bibliográficos
Autor(a) principal: Oliveira, Rodrigo Arruda de
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000fdwf
Texto Completo: http://repositorio.ufsm.br/handle/1/10066
Resumo: During the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results.
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spelling Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetoresPregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectantsEqüinoSêmen congeladoDimetilformamidaGlicerolCriouloEquineFrozen semenDimethylformamidGlycerolCriolloCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADuring the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results.Conselho Nacional de Desenvolvimento Científico e TecnológicoDurante a estação reprodutiva natural de 2005 e 2006 do hemisfério sul, 104 éguas Crioulas foram utilizadas para avaliar a fertilidade do sêmen congelado de cinco garanhões Crioulos. Como diluente usou-se FR4 (Nutricell, Brasil) com 5% de glicerol (GLI) ou 5% de dimetilformamida (DMF) como crioprotetores. O sêmen foi envasado em palhetas de 0,5mL (100x106 espermatozóides/palheta). Examinou-se as éguas em dias alternados por ultra-sonografia transretal para detecção de folículos ≥30mm de diâmetro, quando recebiam hCG (2500UI i.v.). Apos 24h eram examinadas diariamente no Experimento I e a cada 6h no Experimento II. As inseminações com sêmen congelado eram realizadas no fundo do corno ipsislateralis ao folículo dominante. Nas controle, com sêmen fresco no corpo uterino. Utilizou-se um ciclo estral/animal. O diagnóstico de gestação foi conduzido por ultra-sonografia no 15o dia pós-ovulação. No experimento I, a taxa de prenhez foi 11,9% (5/42) e 62,5% (20/32), respectivamente para DMF e sêmen fresco (P<0,0001). No experimento II, a taxa de prenhez foi 40% (4/10), 10% (1/10) e 70% (7/10), respectivamente para DMF, GLI (P>0,05) e sêmen fresco (P<0,0001). Demonstrou-se que o GLI e a DMF podem ser utilizados como crioprotetores para o congelamento do sêmen de garanhões Crioulos. Neste contexto, a DMF apresentou melhores resultados.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaSilva, Carlos Antonio Mondinohttp://lattes.cnpq.br/8811861305924545Neves, Adriana Pireshttp://lattes.cnpq.br/9029302789722486Brass, Karin Ericahttp://lattes.cnpq.br/7502192989660220Oliveira, Rodrigo Arruda de2017-03-022017-03-022007-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfOLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007.http://repositorio.ufsm.br/handle/1/10066ark:/26339/001300000fdwfporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-01-25T18:56:20Zoai:repositorio.ufsm.br:1/10066Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-01-25T18:56:20Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants
title Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
spellingShingle Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
Oliveira, Rodrigo Arruda de
Eqüino
Sêmen congelado
Dimetilformamida
Glicerol
Crioulo
Equine
Frozen semen
Dimethylformamid
Glycerol
Criollo
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
title_full Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
title_fullStr Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
title_full_unstemmed Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
title_sort Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
author Oliveira, Rodrigo Arruda de
author_facet Oliveira, Rodrigo Arruda de
author_role author
dc.contributor.none.fl_str_mv Silva, Carlos Antonio Mondino
http://lattes.cnpq.br/8811861305924545
Neves, Adriana Pires
http://lattes.cnpq.br/9029302789722486
Brass, Karin Erica
http://lattes.cnpq.br/7502192989660220
dc.contributor.author.fl_str_mv Oliveira, Rodrigo Arruda de
dc.subject.por.fl_str_mv Eqüino
Sêmen congelado
Dimetilformamida
Glicerol
Crioulo
Equine
Frozen semen
Dimethylformamid
Glycerol
Criollo
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Eqüino
Sêmen congelado
Dimetilformamida
Glicerol
Crioulo
Equine
Frozen semen
Dimethylformamid
Glycerol
Criollo
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description During the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-27
2017-03-02
2017-03-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007.
http://repositorio.ufsm.br/handle/1/10066
dc.identifier.dark.fl_str_mv ark:/26339/001300000fdwf
identifier_str_mv OLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007.
ark:/26339/001300000fdwf
url http://repositorio.ufsm.br/handle/1/10066
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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