Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000fdwf |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10066 |
Resumo: | During the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results. |
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Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetoresPregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectantsEqüinoSêmen congeladoDimetilformamidaGlicerolCriouloEquineFrozen semenDimethylformamidGlycerolCriolloCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADuring the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results.Conselho Nacional de Desenvolvimento Científico e TecnológicoDurante a estação reprodutiva natural de 2005 e 2006 do hemisfério sul, 104 éguas Crioulas foram utilizadas para avaliar a fertilidade do sêmen congelado de cinco garanhões Crioulos. Como diluente usou-se FR4 (Nutricell, Brasil) com 5% de glicerol (GLI) ou 5% de dimetilformamida (DMF) como crioprotetores. O sêmen foi envasado em palhetas de 0,5mL (100x106 espermatozóides/palheta). Examinou-se as éguas em dias alternados por ultra-sonografia transretal para detecção de folículos ≥30mm de diâmetro, quando recebiam hCG (2500UI i.v.). Apos 24h eram examinadas diariamente no Experimento I e a cada 6h no Experimento II. As inseminações com sêmen congelado eram realizadas no fundo do corno ipsislateralis ao folículo dominante. Nas controle, com sêmen fresco no corpo uterino. Utilizou-se um ciclo estral/animal. O diagnóstico de gestação foi conduzido por ultra-sonografia no 15o dia pós-ovulação. No experimento I, a taxa de prenhez foi 11,9% (5/42) e 62,5% (20/32), respectivamente para DMF e sêmen fresco (P<0,0001). No experimento II, a taxa de prenhez foi 40% (4/10), 10% (1/10) e 70% (7/10), respectivamente para DMF, GLI (P>0,05) e sêmen fresco (P<0,0001). Demonstrou-se que o GLI e a DMF podem ser utilizados como crioprotetores para o congelamento do sêmen de garanhões Crioulos. Neste contexto, a DMF apresentou melhores resultados.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaSilva, Carlos Antonio Mondinohttp://lattes.cnpq.br/8811861305924545Neves, Adriana Pireshttp://lattes.cnpq.br/9029302789722486Brass, Karin Ericahttp://lattes.cnpq.br/7502192989660220Oliveira, Rodrigo Arruda de2017-03-022017-03-022007-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfOLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007.http://repositorio.ufsm.br/handle/1/10066ark:/26339/001300000fdwfporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-01-25T18:56:20Zoai:repositorio.ufsm.br:1/10066Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-01-25T18:56:20Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants |
title |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
spellingShingle |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores Oliveira, Rodrigo Arruda de Eqüino Sêmen congelado Dimetilformamida Glicerol Crioulo Equine Frozen semen Dimethylformamid Glycerol Criollo CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
title_full |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
title_fullStr |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
title_full_unstemmed |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
title_sort |
Índice de prenhez com sêmen congelado de garanhões crioulos usando glicerol ou dimetilformamida como crioprotetores |
author |
Oliveira, Rodrigo Arruda de |
author_facet |
Oliveira, Rodrigo Arruda de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Carlos Antonio Mondino http://lattes.cnpq.br/8811861305924545 Neves, Adriana Pires http://lattes.cnpq.br/9029302789722486 Brass, Karin Erica http://lattes.cnpq.br/7502192989660220 |
dc.contributor.author.fl_str_mv |
Oliveira, Rodrigo Arruda de |
dc.subject.por.fl_str_mv |
Eqüino Sêmen congelado Dimetilformamida Glicerol Crioulo Equine Frozen semen Dimethylformamid Glycerol Criollo CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Eqüino Sêmen congelado Dimetilformamida Glicerol Crioulo Equine Frozen semen Dimethylformamid Glycerol Criollo CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
During the 2005 and 2006 southern hemisphere natural breeding season, 104 Criollo mares were used to evaluate deep frozen semen fertility from 5 Criollo stallions. FR4 was used as extender (Nutricell, Brazil) with 5% glycerol (GLY) or 5% dimethylformamid (DMF) as cryoprotectants. Semen was loaded in 0.5mL straws (100x106 spermatozoa/straw). Mares were examined every other day by ultrasonography until detection of ≥30mm diameter follicles, when hCG was injected (2500UI i.v.). After 24 hours, mares were examined daily in Experiment I, and every 6 hours, in experiment II. Insemination with frozen semen was performed deep in the uterine horn ipsilateralis to the dominant follicle. Controls were inseminated with fresh semen in the body of the uterus. Only one estrus period per mare was used. Pregnancy diagnosis through ultrasonography was performed on the 15th day postovulation. In experiment I, pregnancy rates were 11.9% (5/42) and 62.5% (20/32), respectively for DMF and fresh semen (P<0.0001). In experiment II, pregnancy rates were 40% (4/10), 10% (1/10) and 70% (7/10), respectively for DMF, GLY (P>0.05) and fresh semen (P<0.0001). It was showed that GLY and DMF can be used for frozen of Criollo Stallion s semen. In this context DMF brought better results. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-02-27 2017-03-02 2017-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007. http://repositorio.ufsm.br/handle/1/10066 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000fdwf |
identifier_str_mv |
OLIVEIRA, Rodrigo Arruda de. Pregnancy rates using frozen semen of criollo stallion s with glycerol or dimethylformamid as cryoprotectants. 2007. 47 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2007. ark:/26339/001300000fdwf |
url |
http://repositorio.ufsm.br/handle/1/10066 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1821325909291958272 |