Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6171 |
Resumo: | The aim of this study was to evaluate a formulation of methylene blue (MB) with ethanol to antimicrobial photodynamic therapy (aPDT), used as an adjunct to mechanical treatment of experimental periodontitis induced in rats. Forty rats were randomized into five groups: without periodontitis (CN); and periodontitis without treatment (CP); periodontitis underwent three types of treatment: scaling and root planing and irrigation with saline (RAR); scaling and root planing and aPDT with MB diluted in water to 0.01% (aPDT/water); scaling and root planing and aPDT with MB diluted in 20% ethanol (aPDT/ethanol). Periodontitis was induced by placing ligature on the lower right first molar for seven days. Then, the ligatures were removed and the treatments applied. The animals were euthanized seven days after the treatment. The primary outcome was bone loss in the furcation region of the first molar evaluated histometrically. The secondary outcome was the number of osteoclasts immunohistochemically marked for tartrate-resistant acid phosphatase (TRAP). Differences between groups were assessed by One Way ANOVA and post hoc Bonferroni. Thirty-seven rats were subjected to analysis. All three treatment groups showed statistically lower bone loss and smaller number of TRAP positive osteoclasts compared to the untreated group (CP) (P <0.05). No difference was observed for the outcomes between treatment groups (P> 0.05), and between treatments and the negative control (P> 0.05). Bone loss and osteoclastic activity were not changed by aPDT regardless of the inclusion of ethanol in methylene blue formulation when compared to RAR. Therefore, the aPDT did not result in additional benefit when used as adjunctive method to scaling and root planning, considering the parameters evaluated in this study. |
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2016-03-072016-03-072015-08-26CAMPAGNOLO, Cibele Bruno. PHOTODYNAMIC THERAPY WITH METHYLENE BLUE AND ETHANOL: ALVEOLAR BONE LOSS AND IMMUNE RESPONSE IN RATS WITH PERIODONTITIS. 2015. 89 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/6171The aim of this study was to evaluate a formulation of methylene blue (MB) with ethanol to antimicrobial photodynamic therapy (aPDT), used as an adjunct to mechanical treatment of experimental periodontitis induced in rats. Forty rats were randomized into five groups: without periodontitis (CN); and periodontitis without treatment (CP); periodontitis underwent three types of treatment: scaling and root planing and irrigation with saline (RAR); scaling and root planing and aPDT with MB diluted in water to 0.01% (aPDT/water); scaling and root planing and aPDT with MB diluted in 20% ethanol (aPDT/ethanol). Periodontitis was induced by placing ligature on the lower right first molar for seven days. Then, the ligatures were removed and the treatments applied. The animals were euthanized seven days after the treatment. The primary outcome was bone loss in the furcation region of the first molar evaluated histometrically. The secondary outcome was the number of osteoclasts immunohistochemically marked for tartrate-resistant acid phosphatase (TRAP). Differences between groups were assessed by One Way ANOVA and post hoc Bonferroni. Thirty-seven rats were subjected to analysis. All three treatment groups showed statistically lower bone loss and smaller number of TRAP positive osteoclasts compared to the untreated group (CP) (P <0.05). No difference was observed for the outcomes between treatment groups (P> 0.05), and between treatments and the negative control (P> 0.05). Bone loss and osteoclastic activity were not changed by aPDT regardless of the inclusion of ethanol in methylene blue formulation when compared to RAR. Therefore, the aPDT did not result in additional benefit when used as adjunctive method to scaling and root planning, considering the parameters evaluated in this study.O objetivo do presente estudo foi avaliar uma formulação de azul de metileno (AM) com etanol para terapia fotodinâmica antimicrobiana (TFDa), utilizada como coadjuvante ao tratamento mecânico da periodontite experimental induzida em ratos. Quarenta ratos foram randomizados em cinco grupos: sem periodontite (CN); com periodontite e sem tratamento (CP); com periodontite submetidos a três tipos de tratamento: raspagem e alisamento radicular e irrigação com solução salina (RAR); raspagem e alisamento radicular e TFDa com AM diluído em água a 0,01% (TFD/água); raspagem e alisamento radicular e TFDa com AM diluído em etanol 20% (TFDa/etanol). A periodontite foi induzida pela colocação de ligadura no primeiro molar inferior direito por sete dias. A seguir, as ligaduras foram removidas e os tratamentos aplicados. Os animais foram eutanasiados sete dias após o tratamento. O desfecho primário foi a perda óssea na região de furca do primeiro molar avaliada histometricamente. O desfecho secundário foi o número de osteoclastos imuno-histoquímicamente marcados para fosfatase ácida tartaratoresistente (TRAP). Diferenças entre grupos foram avaliadas por One Way Anova e post hoc Bonferroni. Trinta e sete ratos foram submetidos às análises. Os três grupos de tratamento demonstraram estatisticamente menor perda óssea e menor número de osteoclastos TRAP-positivos, quando comparados ao grupo sem tratamento (CP) (P<0.05). Nenhuma diferença foi verificada para os desfechos entre os grupos de tratamento (P>0,05) e entre os tratamentos e o controle negativo (P>0,05). A perda óssea e atividade osteoclástica não foram alteradas pela TFDa independentemente da inclusão do etanol na formulação do azul de metileno quando comparada a RAR. Portanto, a TFDa não resultou em benefício adicional quando utlizada como método coadjuvante à raspagem e alisamento radicular, considerando os parâmetros avaliados neste estudo.Fundação de Amparo a Pesquisa no Estado do Rio Grande do Sulapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências OdontológicasUFSMBROdontologiaAgentes fotossensibilizantesDoenças periodontaisFotoquimioterapiaOsteoclastosOsteoclastsPeriodontal diseasesPhotosensitizing agentsPhotochemotherapyCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIATerapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontitePhotodynamic therapy with methylene blue and ethanol: alveolar bone loss and immune response in rats with periodontitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisKantorski, Karla Zaninihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731767U6Antoniazzi, Raquel Pippihttp://lattes.cnpq.br/2824180535322900Kommers, Glaucia Denisehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792786U6http://lattes.cnpq.br/6918563924267772Campagnolo, Cibele Bruno4002000000004003003003005001c306d10-e9a7-491c-a4fc-2addf75ee15ca5cc14f0-1342-43ed-a41a-066d21e89213fb597ea5-d23c-4c9f-8ef9-0d33946a77cefcf99dd8-7b07-4f8d-a467-353811148502info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCAMPAGNOLO, CIBELE BRUNO.pdfapplication/pdf1581055http://repositorio.ufsm.br/bitstream/1/6171/1/CAMPAGNOLO%2c%20CIBELE%20BRUNO.pdff43909f8d0fb7ab0ceaeda5753a004beMD51TEXTCAMPAGNOLO, CIBELE BRUNO.pdf.txtCAMPAGNOLO, CIBELE BRUNO.pdf.txtExtracted texttext/plain174912http://repositorio.ufsm.br/bitstream/1/6171/2/CAMPAGNOLO%2c%20CIBELE%20BRUNO.pdf.txt0274199dfd1a7dde73cc69778bdcc70eMD52THUMBNAILCAMPAGNOLO, CIBELE BRUNO.pdf.jpgCAMPAGNOLO, CIBELE BRUNO.pdf.jpgIM Thumbnailimage/jpeg5012http://repositorio.ufsm.br/bitstream/1/6171/3/CAMPAGNOLO%2c%20CIBELE%20BRUNO.pdf.jpg62e6a50f979242f5447986228c4bacf7MD531/61712022-08-18 12:05:15.873oai:repositorio.ufsm.br:1/6171Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-18T15:05:15Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
dc.title.alternative.eng.fl_str_mv |
Photodynamic therapy with methylene blue and ethanol: alveolar bone loss and immune response in rats with periodontitis |
title |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
spellingShingle |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite Campagnolo, Cibele Bruno Agentes fotossensibilizantes Doenças periodontais Fotoquimioterapia Osteoclastos Osteoclasts Periodontal diseases Photosensitizing agents Photochemotherapy CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
title_full |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
title_fullStr |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
title_full_unstemmed |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
title_sort |
Terapia fotodinâmica com azul de metileno e etanol: perda óssea alveolar e resposta imune em ratos com periodontite |
author |
Campagnolo, Cibele Bruno |
author_facet |
Campagnolo, Cibele Bruno |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Kantorski, Karla Zanini |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4731767U6 |
dc.contributor.referee1.fl_str_mv |
Antoniazzi, Raquel Pippi |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2824180535322900 |
dc.contributor.referee2.fl_str_mv |
Kommers, Glaucia Denise |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792786U6 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6918563924267772 |
dc.contributor.author.fl_str_mv |
Campagnolo, Cibele Bruno |
contributor_str_mv |
Kantorski, Karla Zanini Antoniazzi, Raquel Pippi Kommers, Glaucia Denise |
dc.subject.por.fl_str_mv |
Agentes fotossensibilizantes Doenças periodontais Fotoquimioterapia Osteoclastos |
topic |
Agentes fotossensibilizantes Doenças periodontais Fotoquimioterapia Osteoclastos Osteoclasts Periodontal diseases Photosensitizing agents Photochemotherapy CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Osteoclasts Periodontal diseases Photosensitizing agents Photochemotherapy |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The aim of this study was to evaluate a formulation of methylene blue (MB) with ethanol to antimicrobial photodynamic therapy (aPDT), used as an adjunct to mechanical treatment of experimental periodontitis induced in rats. Forty rats were randomized into five groups: without periodontitis (CN); and periodontitis without treatment (CP); periodontitis underwent three types of treatment: scaling and root planing and irrigation with saline (RAR); scaling and root planing and aPDT with MB diluted in water to 0.01% (aPDT/water); scaling and root planing and aPDT with MB diluted in 20% ethanol (aPDT/ethanol). Periodontitis was induced by placing ligature on the lower right first molar for seven days. Then, the ligatures were removed and the treatments applied. The animals were euthanized seven days after the treatment. The primary outcome was bone loss in the furcation region of the first molar evaluated histometrically. The secondary outcome was the number of osteoclasts immunohistochemically marked for tartrate-resistant acid phosphatase (TRAP). Differences between groups were assessed by One Way ANOVA and post hoc Bonferroni. Thirty-seven rats were subjected to analysis. All three treatment groups showed statistically lower bone loss and smaller number of TRAP positive osteoclasts compared to the untreated group (CP) (P <0.05). No difference was observed for the outcomes between treatment groups (P> 0.05), and between treatments and the negative control (P> 0.05). Bone loss and osteoclastic activity were not changed by aPDT regardless of the inclusion of ethanol in methylene blue formulation when compared to RAR. Therefore, the aPDT did not result in additional benefit when used as adjunctive method to scaling and root planning, considering the parameters evaluated in this study. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-08-26 |
dc.date.accessioned.fl_str_mv |
2016-03-07 |
dc.date.available.fl_str_mv |
2016-03-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMPAGNOLO, Cibele Bruno. PHOTODYNAMIC THERAPY WITH METHYLENE BLUE AND ETHANOL: ALVEOLAR BONE LOSS AND IMMUNE RESPONSE IN RATS WITH PERIODONTITIS. 2015. 89 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/6171 |
identifier_str_mv |
CAMPAGNOLO, Cibele Bruno. PHOTODYNAMIC THERAPY WITH METHYLENE BLUE AND ETHANOL: ALVEOLAR BONE LOSS AND IMMUNE RESPONSE IN RATS WITH PERIODONTITIS. 2015. 89 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
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http://repositorio.ufsm.br/handle/1/6171 |
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por |
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