Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/16712 |
Resumo: | During ageing, the cell repair system, including adaptative response to oxidative stress and unfolded protein degradation. In last decades, the increasing life expectancy, added to genetic and ambiental factors, has increasing neurodegenerative diseases incidence, as Alzheimer’s Disease (AD). Amyloid plaques in brain formed by amyloid-β (Aβ) protein aggregation mainly characterize AD. Antioxidant compounds from natural sources have demonstrated beneficial effects, mainly related to aging. In this way, this study aims to evaluate in vivo effects of chronic treatment with the ethanolic extract of Rosmarinus officinalis (eeRo) on aging and Paullinia cupana (GEE) on AD models in the Caernorhabditis elegans nematode, as well as to elucidate its mechanisms. For this, the wild-type strain and transgenic strains with βA expression were used as DA models. Behavioral assays, resistance to different types of stress, longevity, quantification of reactive oxygen species (ROS) and levels of the βA protein and silencing of genes involved in the stress response were performed. The data demonstrated that eeRo treatment decreases ROS levels and increases longevity and stress resistance, in a daf-16, hsf-1 and skn-1 dependent way. Regarding GEE, data demonstrated that the treatment reduced βA levels, delaying its toxic effects through the activation of HSF-1. Thus, in this study, we demonstrated compounds capable of reinforcing the cellular repair systems, offering new alternatives against aging and, related to GEE, prevention against the protein aggregation observed in AD. |
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Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegansEvaluation of natural compounds in the nematode Caernorhabditis elegans aging and Alzheimer disease modelsEnvelhecimentoDoenças neurodegenerativasAlzheimerβ-amilóideCaenorhabditis elegansAgeingNeurodegenerative diseasesAmilóide-βCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICADuring ageing, the cell repair system, including adaptative response to oxidative stress and unfolded protein degradation. In last decades, the increasing life expectancy, added to genetic and ambiental factors, has increasing neurodegenerative diseases incidence, as Alzheimer’s Disease (AD). Amyloid plaques in brain formed by amyloid-β (Aβ) protein aggregation mainly characterize AD. Antioxidant compounds from natural sources have demonstrated beneficial effects, mainly related to aging. In this way, this study aims to evaluate in vivo effects of chronic treatment with the ethanolic extract of Rosmarinus officinalis (eeRo) on aging and Paullinia cupana (GEE) on AD models in the Caernorhabditis elegans nematode, as well as to elucidate its mechanisms. For this, the wild-type strain and transgenic strains with βA expression were used as DA models. Behavioral assays, resistance to different types of stress, longevity, quantification of reactive oxygen species (ROS) and levels of the βA protein and silencing of genes involved in the stress response were performed. The data demonstrated that eeRo treatment decreases ROS levels and increases longevity and stress resistance, in a daf-16, hsf-1 and skn-1 dependent way. Regarding GEE, data demonstrated that the treatment reduced βA levels, delaying its toxic effects through the activation of HSF-1. Thus, in this study, we demonstrated compounds capable of reinforcing the cellular repair systems, offering new alternatives against aging and, related to GEE, prevention against the protein aggregation observed in AD.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESDurante o envelhecimento, os sistemas de reparo, incluindo a resposta adaptativa ao estresse oxidativo e a degradação de proteínas danificadas, diminuem. Nas últimas décadas, o aumento contínuo na expectativa de vida na população, somado a fatores genéticos e ambientais, tem aumentado significativamente a incidência de doenças relacionadas ao envelhecimento, em especial as neurodegenerativas, como a Doença de Alzheimer (DA). A neuropatologia da DA é caracterizada principalmente pela presença de placas amiloides no cérebro formadas pela agregação da proteína β-amiloide (βA). Compostos antioxidantes provindos de fontes naturais têm demostrados diversos efeitos benéficos, principalmente relacionados ao envelhecimento. Além disso, vias de sinalização que regulam a expressão gênica em resposta ao estresse têm sido alvos na pesquisa por genes envolvidos na longevidade. Desta forma, este trabalho tem como objetivos avaliar os efeitos in vivo do tratamento crônico com o extrato etanólico de Rosmarinus officinalis (eeRo) no envelhecimento e de Paullinia cupana (GEE) em modelos de DA no nematódeo Caernorhabditis elegans, bem como elucidar seus mecanismos. Para isso foi utilizada a cepa selvagem e cepas transgênicas com expressão da βA como modelos de DA. Foram realizados ensaios comportamentais, de resistência a diferentes tipos de estresse, longevidade, quantificação de espécies reativas de oxigênio (ERO) e dos níveis da proteína βA e silenciamento de genes envolvidos na resposta ao estresse. Os dados demonstraram que o tratamento com eeRo diminui os níveis de ERO e aumenta a longevidade e a resistência ao estresse, de forma dependente dos fatores de transcrição daf-16, hsf-1 e skn-1. Em relação ao GEE, os dados demonstraram que o tratamento reduziu os níveis da βA, retardando seus efeitos tóxicos através da ativação da HSF-1. Assim, neste estudo foram demonstrados compostos capazes de reforçar os sistemas de reparo celular, oferecendo novas alternativas contra o envelhecimento e, ainda, no caso do GEE, prevenção contra a agregação proteica observada na DA.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasSoares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092Monserrat, José Maríahttp://lattes.cnpq.br/8106459529828166Ávila, Daiana Silva dehttp://lattes.cnpq.br/4355211015887363Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Schuch, André Passagliahttp://lattes.cnpq.br/4932611269622766Zamberlan, Daniele Coradini2019-05-31T15:22:55Z2019-05-31T15:22:55Z2018-09-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/16712porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-06-01T06:02:09Zoai:repositorio.ufsm.br:1/16712Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-06-01T06:02:09Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans Evaluation of natural compounds in the nematode Caernorhabditis elegans aging and Alzheimer disease models |
title |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
spellingShingle |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans Zamberlan, Daniele Coradini Envelhecimento Doenças neurodegenerativas Alzheimer β-amilóide Caenorhabditis elegans Ageing Neurodegenerative diseases Amilóide-β CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
title_full |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
title_fullStr |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
title_full_unstemmed |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
title_sort |
Efeito de compostos naturais no envelhecimento e em modelos de doença de Alzheimer no nematódeo Caernorhabditis elegans |
author |
Zamberlan, Daniele Coradini |
author_facet |
Zamberlan, Daniele Coradini |
author_role |
author |
dc.contributor.none.fl_str_mv |
Soares, Félix Alexandre Antunes http://lattes.cnpq.br/8752453650114092 Monserrat, José María http://lattes.cnpq.br/8106459529828166 Ávila, Daiana Silva de http://lattes.cnpq.br/4355211015887363 Fachinetto, Roselei http://lattes.cnpq.br/7203076675431306 Schuch, André Passaglia http://lattes.cnpq.br/4932611269622766 |
dc.contributor.author.fl_str_mv |
Zamberlan, Daniele Coradini |
dc.subject.por.fl_str_mv |
Envelhecimento Doenças neurodegenerativas Alzheimer β-amilóide Caenorhabditis elegans Ageing Neurodegenerative diseases Amilóide-β CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Envelhecimento Doenças neurodegenerativas Alzheimer β-amilóide Caenorhabditis elegans Ageing Neurodegenerative diseases Amilóide-β CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
During ageing, the cell repair system, including adaptative response to oxidative stress and unfolded protein degradation. In last decades, the increasing life expectancy, added to genetic and ambiental factors, has increasing neurodegenerative diseases incidence, as Alzheimer’s Disease (AD). Amyloid plaques in brain formed by amyloid-β (Aβ) protein aggregation mainly characterize AD. Antioxidant compounds from natural sources have demonstrated beneficial effects, mainly related to aging. In this way, this study aims to evaluate in vivo effects of chronic treatment with the ethanolic extract of Rosmarinus officinalis (eeRo) on aging and Paullinia cupana (GEE) on AD models in the Caernorhabditis elegans nematode, as well as to elucidate its mechanisms. For this, the wild-type strain and transgenic strains with βA expression were used as DA models. Behavioral assays, resistance to different types of stress, longevity, quantification of reactive oxygen species (ROS) and levels of the βA protein and silencing of genes involved in the stress response were performed. The data demonstrated that eeRo treatment decreases ROS levels and increases longevity and stress resistance, in a daf-16, hsf-1 and skn-1 dependent way. Regarding GEE, data demonstrated that the treatment reduced βA levels, delaying its toxic effects through the activation of HSF-1. Thus, in this study, we demonstrated compounds capable of reinforcing the cellular repair systems, offering new alternatives against aging and, related to GEE, prevention against the protein aggregation observed in AD. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-05 2019-05-31T15:22:55Z 2019-05-31T15:22:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/16712 |
url |
http://repositorio.ufsm.br/handle/1/16712 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922057910747136 |