Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10030 |
Resumo: | The present study describes the evaluation of the effect of supplementation with selenium and vitamin E on the hemogram and protein and oxidative profiles of lambs experimentally infected with Haemonchus contortus (HC). Thirty female lambs were divided into four groups as follows: G1 (n = 10): infected animals; G2 (n =10): infected and supplemented; G3 (n = 5): control; G4 (n = 5): non-infected and supplemented. Groups 1 and 2 received 500 HC larvae (L3) orally for a period of 20 days, with 2-day intervals between doses. Supplementation in groups 2 and 4 was performed on day zero by injecting 0.1 mg kg-1 of sodium selenite (1.67%) and 2,000 IU vitamin E through the intramuscular (IM) route. Vitamin E alone was injected once again on day 30. In addition to blood collections for performing the hemograms, the numbers of eggs per grams of feces (EPG) were also determined. Considering the number of erythrocytes, volume of globular proteins, hemoglobin level, total leukocytes and lymphocytes, the main differences were observed when the parasitized groups were compared to the supplemented, non-parasitized group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their erythrogram when they are parasitized with HC. The leukogram showed higher segmented neutrophil numbers in the supplemented groups. On the other hand, vitamin E and selenium increased hematological parameters in animals not infected with HC. In considering the levels of total proteins, albumin, beta and gamma globulins, the main differences were observed when the parasitized groups were compared to the supplemented, non-infected group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their blood protein levels when they are parasitized with HC. The non-parasitized, supplemented animals sustained better glutathione peroxidase (GSH-Px) values up to the 60th day of the experiment. No oxidative stress state took place since an increase in GSH-Px levels exerted a compensatory effect when thiobarbituric acid reactive substances (TBARS) levels rose. |
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2009-02-122009-02-122008-12-18NICOLODI, Paula Rocha Sampaio Juchem. Effect of selenium and vitamin E on the hemogram, proteinogram and metabolism oxidative of lambs experimentally infected with Haemonchus contortus. 2008. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/10030The present study describes the evaluation of the effect of supplementation with selenium and vitamin E on the hemogram and protein and oxidative profiles of lambs experimentally infected with Haemonchus contortus (HC). Thirty female lambs were divided into four groups as follows: G1 (n = 10): infected animals; G2 (n =10): infected and supplemented; G3 (n = 5): control; G4 (n = 5): non-infected and supplemented. Groups 1 and 2 received 500 HC larvae (L3) orally for a period of 20 days, with 2-day intervals between doses. Supplementation in groups 2 and 4 was performed on day zero by injecting 0.1 mg kg-1 of sodium selenite (1.67%) and 2,000 IU vitamin E through the intramuscular (IM) route. Vitamin E alone was injected once again on day 30. In addition to blood collections for performing the hemograms, the numbers of eggs per grams of feces (EPG) were also determined. Considering the number of erythrocytes, volume of globular proteins, hemoglobin level, total leukocytes and lymphocytes, the main differences were observed when the parasitized groups were compared to the supplemented, non-parasitized group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their erythrogram when they are parasitized with HC. The leukogram showed higher segmented neutrophil numbers in the supplemented groups. On the other hand, vitamin E and selenium increased hematological parameters in animals not infected with HC. In considering the levels of total proteins, albumin, beta and gamma globulins, the main differences were observed when the parasitized groups were compared to the supplemented, non-infected group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their blood protein levels when they are parasitized with HC. The non-parasitized, supplemented animals sustained better glutathione peroxidase (GSH-Px) values up to the 60th day of the experiment. No oxidative stress state took place since an increase in GSH-Px levels exerted a compensatory effect when thiobarbituric acid reactive substances (TBARS) levels rose.O presente estudo descreve a avaliação da influência da suplementação com selênio e vitamina E sobre o hemograma, perfil protéico e oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus (HC). Trinta cordeiros, fêmeas, foram distribuídos em quatro grupos, sendo: G1 (n = 10): animais infectados; G2 (n =10): infectados e suplementados; G3 (n = 5): controle; G4 (n = 5): não infectados e suplementados. Os grupos 1 e 2 receberam 500 larvas de HC (L3), via oral, por um período de 20 dias, com intervalo de dois dias entre as doses. A suplementação nos grupos 2 e 4 foi realizada no dia zero com 0,1mg kg-1 de Selenito de sódio (1,67%) e com 2.000 UI de vitamina E por via intramuscular (IM). Somente a vitamina E foi reaplicada no dia 30. Além das coletas de sangue para a realização do hemograma, foram determinados números de ovos por grama de fezes (OPG). Em relação ao número de hemácias, volume globular, hemoglobina, leucócitos totais e linfócitos, as principais diferenças foram observadas quando os grupos parasitados foram comparados com o grupo somente suplementado, este, mantendo valores mais elevados. Conclui-se que não há influência da suplementação dos cordeiros com vitamina E e selênio sob os parâmetros de eritrograma quando estes se encontram parasitados por HC. No leucograma pôde-se observar valores mais elevados de neutrófilos segmentados nos grupos suplementados. No entanto, a vitamina E e o selênio aumentaram parâmetros hematológicos em animais não infectados com HC. Em relação aos valores de proteínas totais, albumina, betaglobulina, gamaglobulina, as principais diferenças foram observadas quando os grupos parasitados foram comparados com o grupo somente suplementado, este, mantendo valores mais elevados.Conclui-se que não há influência da suplementação dos cordeiros com vitamina E e selênio sob os parâmetros protéicos quando estes se encontram parasitados por HC. Os animais suplementados e não parasitados mantiveram melhores valores de Glutationaperoxidase (GSH-Px) até o 60º dia de experimento. Não houve situação de estresse oxidativo visto que, quando aumentou os teores de TBARS houve um efeito compensatório gerado pelo aumento da GSH-Px.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaOvinosHaemonchus contortusHemogramaProteinogramaMetabolismo oxidativoSheepHaemonchus contortusHemogramProteinogramMetabolism oxidativeCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAEfeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortusEffect of selenium and vitamin E on the hemogram, proteinogram and metabolism oxidative of lambs experimentally infected with Haemonchus contortusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLeal, Marta Lizandra do Rêgohttp://lattes.cnpq.br/6859797230596402Lopes, Sonia Teresinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Sangioni, Luis Antoniohttp://lattes.cnpq.br/8056805667740451http://lattes.cnpq.br/6109484081894055Nicolodi, Paula Rocha Sampaio Juchem500500000007400500300500500ac987a2c-3a2a-4ac9-a240-719530d84a377c5cd2e2-b45d-4ea1-a0f6-94b8fcd19b3a82215a5f-66fa-4814-aac6-5385ebd0c8aa47b3fb7c-79e3-4fce-8565-4d0c754e0310info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPAULAROCHASAMPAIOJUCHEMNICOLODI.pdfapplication/pdf343415http://repositorio.ufsm.br/bitstream/1/10030/1/PAULAROCHASAMPAIOJUCHEMNICOLODI.pdfeade5059346d3db83691640e6f767f3cMD51TEXTPAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.txtPAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.txtExtracted texttext/plain119517http://repositorio.ufsm.br/bitstream/1/10030/2/PAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.txta0f9e451475daee8a769e0e0cffd9ec4MD52THUMBNAILPAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.jpgPAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.jpgIM Thumbnailimage/jpeg4992http://repositorio.ufsm.br/bitstream/1/10030/3/PAULAROCHASAMPAIOJUCHEMNICOLODI.pdf.jpgd6824525f90691f9663e6fb64687883fMD531/100302022-01-10 14:54:36.306oai:repositorio.ufsm.br:1/10030Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-10T17:54:36Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
dc.title.alternative.eng.fl_str_mv |
Effect of selenium and vitamin E on the hemogram, proteinogram and metabolism oxidative of lambs experimentally infected with Haemonchus contortus |
title |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
spellingShingle |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus Nicolodi, Paula Rocha Sampaio Juchem Ovinos Haemonchus contortus Hemograma Proteinograma Metabolismo oxidativo Sheep Haemonchus contortus Hemogram Proteinogram Metabolism oxidative CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
title_full |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
title_fullStr |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
title_full_unstemmed |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
title_sort |
Efeito do selênio e vitamina E sobre o hemograma, proteínograma e metabolismo oxidativo de cordeiros infectados experimentalmente pelo Haemonchus contortus |
author |
Nicolodi, Paula Rocha Sampaio Juchem |
author_facet |
Nicolodi, Paula Rocha Sampaio Juchem |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Leal, Marta Lizandra do Rêgo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6859797230596402 |
dc.contributor.referee1.fl_str_mv |
Lopes, Sonia Teresinha dos Anjos |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8059723754130756 |
dc.contributor.referee2.fl_str_mv |
Sangioni, Luis Antonio |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8056805667740451 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6109484081894055 |
dc.contributor.author.fl_str_mv |
Nicolodi, Paula Rocha Sampaio Juchem |
contributor_str_mv |
Leal, Marta Lizandra do Rêgo Lopes, Sonia Teresinha dos Anjos Sangioni, Luis Antonio |
dc.subject.por.fl_str_mv |
Ovinos Haemonchus contortus Hemograma Proteinograma Metabolismo oxidativo |
topic |
Ovinos Haemonchus contortus Hemograma Proteinograma Metabolismo oxidativo Sheep Haemonchus contortus Hemogram Proteinogram Metabolism oxidative CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Sheep Haemonchus contortus Hemogram Proteinogram Metabolism oxidative |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The present study describes the evaluation of the effect of supplementation with selenium and vitamin E on the hemogram and protein and oxidative profiles of lambs experimentally infected with Haemonchus contortus (HC). Thirty female lambs were divided into four groups as follows: G1 (n = 10): infected animals; G2 (n =10): infected and supplemented; G3 (n = 5): control; G4 (n = 5): non-infected and supplemented. Groups 1 and 2 received 500 HC larvae (L3) orally for a period of 20 days, with 2-day intervals between doses. Supplementation in groups 2 and 4 was performed on day zero by injecting 0.1 mg kg-1 of sodium selenite (1.67%) and 2,000 IU vitamin E through the intramuscular (IM) route. Vitamin E alone was injected once again on day 30. In addition to blood collections for performing the hemograms, the numbers of eggs per grams of feces (EPG) were also determined. Considering the number of erythrocytes, volume of globular proteins, hemoglobin level, total leukocytes and lymphocytes, the main differences were observed when the parasitized groups were compared to the supplemented, non-parasitized group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their erythrogram when they are parasitized with HC. The leukogram showed higher segmented neutrophil numbers in the supplemented groups. On the other hand, vitamin E and selenium increased hematological parameters in animals not infected with HC. In considering the levels of total proteins, albumin, beta and gamma globulins, the main differences were observed when the parasitized groups were compared to the supplemented, non-infected group, the latter exhibiting higher values. It is concluded that supplementation of lambs with vitamin E and selenium has no influence on their blood protein levels when they are parasitized with HC. The non-parasitized, supplemented animals sustained better glutathione peroxidase (GSH-Px) values up to the 60th day of the experiment. No oxidative stress state took place since an increase in GSH-Px levels exerted a compensatory effect when thiobarbituric acid reactive substances (TBARS) levels rose. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-12-18 |
dc.date.accessioned.fl_str_mv |
2009-02-12 |
dc.date.available.fl_str_mv |
2009-02-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NICOLODI, Paula Rocha Sampaio Juchem. Effect of selenium and vitamin E on the hemogram, proteinogram and metabolism oxidative of lambs experimentally infected with Haemonchus contortus. 2008. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10030 |
identifier_str_mv |
NICOLODI, Paula Rocha Sampaio Juchem. Effect of selenium and vitamin E on the hemogram, proteinogram and metabolism oxidative of lambs experimentally infected with Haemonchus contortus. 2008. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
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http://repositorio.ufsm.br/handle/1/10030 |
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por |
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Universidade Federal de Santa Maria |
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UFSM |
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BR |
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Medicina Veterinária |
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Universidade Federal de Santa Maria |
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