Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans

Detalhes bibliográficos
Autor(a) principal: Machado, Marina Lopes
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/28350
Resumo: Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways.
id UFSM_b1885359148cb27a67ee121cf31c5423
oai_identifier_str oai:repositorio.ufsm.br:1/28350
network_acronym_str UFSM
network_name_str Biblioteca Digital de Teses e Dissertações do UFSM
repository_id_str
spelling 2023-03-23T15:14:09Z2023-03-23T15:14:09Z2016-02-25http://repositorio.ufsm.br/handle/1/28350Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways.Ilex paraguariensis é uma planta amplamente consumida no sul do Brasil, Uruguai e Argentina, e bem caracterizada por suas propriedades antioxidantes e estimulantes, e pode ser usada como um modulador dos depósitos de gordura para o controle da obesidade, um problema mundial. Portanto, o efeito do consumo crônico de Ilex paraguariensis no metabolismo de gordura foi investigado usando o nematódeo Caenorhabditis elegans. Primeiramente, foi realizado a identificação e quantificação por DAD-HPLC, dos constituintes do extrato aquoso de Ilex paraguariensis. Posteriormente, os nematódeos foram tratados com 1mg/mL de I. paraguariensis a partir do estágio L1 até a vida adulta. A concentração utilizada do extrato foi escolhida baseada em suas propriedades antioxidantes contra juglone, um gerador endógeno de espécies reativas de oxigênio, uma vez que estas estão aumentadas no tecido adiposo de pessoas obesas. O acumulo de lipídeos, o gasto total de energia e alguns parâmetros comportamentais foram analisados. Ilex paraguariensis diminuiu a fluorescência do corante BODIPY em 63,36% comparado ao controle sem afetar os comportamentos relacionados ao balanço energético. O gasto energético total da cepa selvagem N2, das mutantes nhr-49 e ador-1 foi quantificado através do consumo de oxigênio, Ilex paraguariensis aumentou o consumo de O2 apenas na cepa selvagem. Em conclusão, Ilex paraguariensis diminuiu os depósitos de lipídeos e aumentou o consumo de oxigênio na cepa selvagem, de uma forma dependente das vias NHR-49 e ADOR-1.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMetabolismo de gorduraLipídeosProdutos naturaisConsumo de oxigênioErva-mateFat metabolismLipidsNatural productsOxygen consumptionYerba mateCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegansEvaluation of the effect of chronic exposition to Ilex paraguariensis aqueous extract on the behavior and lipid profile in Caenorhabditis elegansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSoares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092Oliveira, Sara Marchesan deWagner, Carolinehttp://lattes.cnpq.br/0382013676927848Machado, Marina Lopes2008001000096006006006006003aef53b7-b173-4c56-a944-bed2de5e5dda121a3386-f146-43dc-9b04-c78a9b47d7d4ae9ccccd-6c23-46a1-abda-fa450ef31e4f75ee513b-7658-4252-ad23-0af0d13b7c8ereponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGBT_2016_ MACHADO_MARINA.pdfDIS_PPGBT_2016_ MACHADO_MARINA.pdfDissertação de mestradoapplication/pdf1153954http://repositorio.ufsm.br/bitstream/1/28350/1/DIS_PPGBT_2016_%20MACHADO_MARINA.pdfad58c9b0ef3620ee59e979b1ed96330eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/28350/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/28350/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/283502023-03-23 12:14:09.269oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-03-23T15:14:09Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
dc.title.alternative.eng.fl_str_mv Evaluation of the effect of chronic exposition to Ilex paraguariensis aqueous extract on the behavior and lipid profile in Caenorhabditis elegans
title Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
spellingShingle Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
Machado, Marina Lopes
Metabolismo de gordura
Lipídeos
Produtos naturais
Consumo de oxigênio
Erva-mate
Fat metabolism
Lipids
Natural products
Oxygen consumption
Yerba mate
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
title_full Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
title_fullStr Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
title_full_unstemmed Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
title_sort Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
author Machado, Marina Lopes
author_facet Machado, Marina Lopes
author_role author
dc.contributor.advisor1.fl_str_mv Soares, Félix Alexandre Antunes
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8752453650114092
dc.contributor.referee1.fl_str_mv Oliveira, Sara Marchesan de
dc.contributor.referee2.fl_str_mv Wagner, Caroline
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0382013676927848
dc.contributor.author.fl_str_mv Machado, Marina Lopes
contributor_str_mv Soares, Félix Alexandre Antunes
Oliveira, Sara Marchesan de
Wagner, Caroline
dc.subject.por.fl_str_mv Metabolismo de gordura
Lipídeos
Produtos naturais
Consumo de oxigênio
Erva-mate
topic Metabolismo de gordura
Lipídeos
Produtos naturais
Consumo de oxigênio
Erva-mate
Fat metabolism
Lipids
Natural products
Oxygen consumption
Yerba mate
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.eng.fl_str_mv Fat metabolism
Lipids
Natural products
Oxygen consumption
Yerba mate
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways.
publishDate 2016
dc.date.issued.fl_str_mv 2016-02-25
dc.date.accessioned.fl_str_mv 2023-03-23T15:14:09Z
dc.date.available.fl_str_mv 2023-03-23T15:14:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/28350
url http://repositorio.ufsm.br/handle/1/28350
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 200800100009
dc.relation.confidence.fl_str_mv 600
600
600
600
600
dc.relation.authority.fl_str_mv 3aef53b7-b173-4c56-a944-bed2de5e5dda
121a3386-f146-43dc-9b04-c78a9b47d7d4
ae9ccccd-6c23-46a1-abda-fa450ef31e4f
75ee513b-7658-4252-ad23-0af0d13b7c8e
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Bioquímica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Biblioteca Digital de Teses e Dissertações do UFSM
collection Biblioteca Digital de Teses e Dissertações do UFSM
bitstream.url.fl_str_mv http://repositorio.ufsm.br/bitstream/1/28350/1/DIS_PPGBT_2016_%20MACHADO_MARINA.pdf
http://repositorio.ufsm.br/bitstream/1/28350/2/license_rdf
http://repositorio.ufsm.br/bitstream/1/28350/3/license.txt
bitstream.checksum.fl_str_mv ad58c9b0ef3620ee59e979b1ed96330e
4460e5956bc1d1639be9ae6146a50347
2f0571ecee68693bd5cd3f17c1e075df
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
_version_ 1801485138647842816

Registros relacionados