Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos

Detalhes bibliográficos
Autor(a) principal: Giacometi, Marjorie de
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/20748
Resumo: Brazil has the third largest herd of horses in the world, with 5 million heads, generating approximately US $ 7.5 billion per year and consequently using direct. For this reason, it is important to maintain the health and well being of these animals. However, parasitic diseases in horses delay their development and may lead the animal to death, causing serious economic losses. The anti-helminth resistance installed in several herds is a serious problem in veterinary medicine, since antiparasitic treatments do not have the expected effect on these infections or cause high toxicity in the animal. The fungus Pleurotus spp., known worldwide as “oyster mushroom”, an edible mushroom, has over 1000 species already described. It is known that this fungus is capable of producing bioactive compounds that help to combat pathologies and improve the well-being of people, in addition to being considered a nematophagous fungus capable of producing toxins effective in the control of various parasites. The object of this study was to evaluate the activity of different species of Pleurotus spp. in vitro on naturally infected equine cyathostomes eggs. In search of this, the aqueous extract of Pleurotus ostreatus, Pleurotus florida and Pleurotus djamor were used. Eggs were recovered and incubated (27 ° C) along with extracts at increasing concentrations of 0.62%, 1.25%, 2.5%, 5%, 10% and a negative control group with distilled water. The reading was done in 48h in inverted microscope. All concentrations showed effects on hatchability. It was observed that P. florida was more effective than the other Pleurotus, inhibiting hatchability by up to 92.19% at the highest concentration (10%), with LC50 of 2.13% (± 0.12), while inhibition P. ostreatus was 55.46%, coLC50 7.44% (± 0.09), while P. djamor 23.67% at the same concentration. The use of this fungus against cyathostomines shows great potential as a biological control of the nematode.
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spelling Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinosIn vitro activity of Pleurotus ostreatus, Pleurotus florida and Pleurotus djamor in equine cyathostomines eggsEquinosCiatostomíneosPleurotus sppControle biológicoHorsesCyathostomesBiologic controlCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIABrazil has the third largest herd of horses in the world, with 5 million heads, generating approximately US $ 7.5 billion per year and consequently using direct. For this reason, it is important to maintain the health and well being of these animals. However, parasitic diseases in horses delay their development and may lead the animal to death, causing serious economic losses. The anti-helminth resistance installed in several herds is a serious problem in veterinary medicine, since antiparasitic treatments do not have the expected effect on these infections or cause high toxicity in the animal. The fungus Pleurotus spp., known worldwide as “oyster mushroom”, an edible mushroom, has over 1000 species already described. It is known that this fungus is capable of producing bioactive compounds that help to combat pathologies and improve the well-being of people, in addition to being considered a nematophagous fungus capable of producing toxins effective in the control of various parasites. The object of this study was to evaluate the activity of different species of Pleurotus spp. in vitro on naturally infected equine cyathostomes eggs. In search of this, the aqueous extract of Pleurotus ostreatus, Pleurotus florida and Pleurotus djamor were used. Eggs were recovered and incubated (27 ° C) along with extracts at increasing concentrations of 0.62%, 1.25%, 2.5%, 5%, 10% and a negative control group with distilled water. The reading was done in 48h in inverted microscope. All concentrations showed effects on hatchability. It was observed that P. florida was more effective than the other Pleurotus, inhibiting hatchability by up to 92.19% at the highest concentration (10%), with LC50 of 2.13% (± 0.12), while inhibition P. ostreatus was 55.46%, coLC50 7.44% (± 0.09), while P. djamor 23.67% at the same concentration. The use of this fungus against cyathostomines shows great potential as a biological control of the nematode.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqO Brasil possui o terceiro maior rebanho de equinos do mundo, contando com 5 milhões de cabeça, gerando aproximadamente 7,5 bilhões por ano e consequentemente empregos diretos. Por esse motivo é importante manter a saúde e bem estar desses animais. No entanto, as doenças parasitárias em equinos atrasam seu desenvolvimento podendo levar o animal a óbito, ocasionando sérias perdas econômicas. A resistência antihelmíntica instalada em diversos rebanhos é um sério problema na medicina veterinária, uma vez que os tratamentos antiparasitários não surtem efeito esperado nessas infecções ou causam alta toxidade no animal. O gênero Pleurotus, é um fungo conhecido mundialmente como shimeji, um cogumelo comestível que possui mais de 1000 espécies descritas. Sabe-se que esse fungo é capaz de produzir compostos bioativos que auxiliam no combate a patologias e melhoram o bem estar de pessoas, além de ser considerado um fungo nematófago capaz de produzir toxinas eficazes no controle de diversos parasitos. O objetivo deste estudo foi avaliar a atividade de três espécies de Pleurotus in vitro sobre ovos de ciatostomíneos de equinos naturalmente infectados. Para esse experimento, foram realizados extratos aquosos das espécies Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor. Os ovos de ciatostomíneos foram recuperados e incubados (27º C) juntamente com os extratos, nas concentrações crescentes de 0,62%, 1,25%, 2,5%, 5%, 10%. O grupo controle negativo foi realizado somente com água destilada. A leitura foi realizada após 48h em microscópio invertido. Todas as concentrações apresentaram efeitos na eclodibilidade. Observou-se que o P. florida foi mais efetivo que os demais Pleurotus, inibindo a eclodibilidade em até 92,19% na maior concentração (10%), com CL50 de 2,13% (±0,12), já a inibição do P. ostreatus foi de 55,46%, com CL50 7,44% (±0,09), enquanto o P. djamor 23,67% na mesma concentração. A utilização desse fungo em ovos de ciatostomíneos in vitro demonstrou atividade ovicida sendo necessário maiores estudos a fim de verificar o seu potencial para uso em controle biológico desse nematoide.Universidade Federal de Santa MariaBrasilMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaCentro de Ciências RuraisMonteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Baldissera, Matheus DellaméaOurique, Aline FerreiraGiacometi, Marjorie de2021-04-30T18:39:52Z2021-04-30T18:39:52Z2020-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/20748porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-05-01T06:02:12Zoai:repositorio.ufsm.br:1/20748Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-05-01T06:02:12Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
In vitro activity of Pleurotus ostreatus, Pleurotus florida and Pleurotus djamor in equine cyathostomines eggs
title Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
spellingShingle Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
Giacometi, Marjorie de
Equinos
Ciatostomíneos
Pleurotus spp
Controle biológico
Horses
Cyathostomes
Biologic control
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
title_full Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
title_fullStr Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
title_full_unstemmed Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
title_sort Atividade in vitro de Pleurotus ostreatus, Pleurotus florida e Pleurotus djamor em ovos de ciatostomíneos de equinos
author Giacometi, Marjorie de
author_facet Giacometi, Marjorie de
author_role author
dc.contributor.none.fl_str_mv Monteiro, Silvia Gonzalez
http://lattes.cnpq.br/3762606653182779
Baldissera, Matheus Dellaméa
Ourique, Aline Ferreira
dc.contributor.author.fl_str_mv Giacometi, Marjorie de
dc.subject.por.fl_str_mv Equinos
Ciatostomíneos
Pleurotus spp
Controle biológico
Horses
Cyathostomes
Biologic control
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Equinos
Ciatostomíneos
Pleurotus spp
Controle biológico
Horses
Cyathostomes
Biologic control
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description Brazil has the third largest herd of horses in the world, with 5 million heads, generating approximately US $ 7.5 billion per year and consequently using direct. For this reason, it is important to maintain the health and well being of these animals. However, parasitic diseases in horses delay their development and may lead the animal to death, causing serious economic losses. The anti-helminth resistance installed in several herds is a serious problem in veterinary medicine, since antiparasitic treatments do not have the expected effect on these infections or cause high toxicity in the animal. The fungus Pleurotus spp., known worldwide as “oyster mushroom”, an edible mushroom, has over 1000 species already described. It is known that this fungus is capable of producing bioactive compounds that help to combat pathologies and improve the well-being of people, in addition to being considered a nematophagous fungus capable of producing toxins effective in the control of various parasites. The object of this study was to evaluate the activity of different species of Pleurotus spp. in vitro on naturally infected equine cyathostomes eggs. In search of this, the aqueous extract of Pleurotus ostreatus, Pleurotus florida and Pleurotus djamor were used. Eggs were recovered and incubated (27 ° C) along with extracts at increasing concentrations of 0.62%, 1.25%, 2.5%, 5%, 10% and a negative control group with distilled water. The reading was done in 48h in inverted microscope. All concentrations showed effects on hatchability. It was observed that P. florida was more effective than the other Pleurotus, inhibiting hatchability by up to 92.19% at the highest concentration (10%), with LC50 of 2.13% (± 0.12), while inhibition P. ostreatus was 55.46%, coLC50 7.44% (± 0.09), while P. djamor 23.67% at the same concentration. The use of this fungus against cyathostomines shows great potential as a biological control of the nematode.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-05
2021-04-30T18:39:52Z
2021-04-30T18:39:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/20748
url http://repositorio.ufsm.br/handle/1/20748
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
Centro de Ciências Rurais
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Medicina Veterinária
UFSM
Programa de Pós-Graduação em Medicina Veterinária
Centro de Ciências Rurais
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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