Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)

Detalhes bibliográficos
Autor(a) principal: Toni, Cândida
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000s82q
Texto Completo: http://repositorio.ufsm.br/handle/1/11132
Resumo: The intensive use and/or inadequate management of pesticides have led to contamination of the aquatic ecosystem, reaching the biota living there, including the fish. Pesticides can affect toxicological parameters these animals, harming their survival. In this way, the aim this study has to evaluate biochemistry changes in carps (Cyprinus carpio) after exposure to a commercial formulation of the fungicide tebuconazole (Folicur®). At first, we performed a test of acute toxicity to determine the LC50 (96h) of tebuconazole for C. carpio. The animals were exposed to concentrations 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L for 96 hours. After this period, were anesthetized and euthanized. Using the whole-body method, we investigated the levels of TBARS, protein carbonyls, activities of enzymes GST, CAT, SOD and AChE, levels of non-enzymatic antioxidants GSH and AsA and metabolic parameters, among which, lactate, glycogen, glucose, ammonia, amino acids and protein. Secondly, fish were exposed to the fungicide during seven days, both in field conditions (rice paddy field) as a laboratory. The concentrations used in the laboratory (33.5 and 36.2 mg/L) were close to the concentration used for rice farming (31.9 mg/L). After the experimental periods, we sampled brain, liver and muscle of fish. The biochemical parameters analyzed were as follows: TBARS levels in brain, liver and muscle, protein carbonyl in liver, enzyme activities CAT and GST in liver and AChE in brain and muscle. In the first experiment was obtained as LC50 (96h) of tebuconazole for C. carpio concentration 2.37 mg/L. The body levels of TBARS showed to be high at all concentrations tested. In contrast, the enzymes GST, CAT and SOD showed reduction in their activities. The levels of GSH and AsA were also decreased after the same period. Regarding the activity of AChE and the levels of protein carbonyls, there were no statistically significant changes. Among the metabolic parameters, there was an increase in the levels of glycogen and glucose concentration 1.5 mg/L, whereas protein levels were reduced at concentrations 2.0 and 2.5 mg/L. In the second experiment, we observed increased levels of TBARS in brain only in the field condition, while in liver and muscle this increase was observed both in field and laboratory. Likewise, the levels of protein carbonyls increased in both experimental conditions. The activity of AChE in brain was increased only in the field condition, whereas in the laboratory no significant change was observed. The activities of CAT, GST and AChE in muscle showed no significant change in both experimental conditions. The results of this research, considering the two experiments show that this fungicide causes disorders in antioxidants and metabolic parameters in fish exposed, indicating the occurrence of oxidative stress, which can compromise their survival in the wild. The evaluation of TBARS levels and protein carbonyl in the liver of carp can be used as a biomarker of exposure to tebuconazole.
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spelling Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)Biochemical response in carp (Cyprinus carpio) exposed to fungicide tebuconazole (folicur® 200 ec)Cyprinus carpioEstresse oxidativoTebuconazoleToxicidadeCyprinus carpioOxidative stressTebuconazoleToxicityCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAThe intensive use and/or inadequate management of pesticides have led to contamination of the aquatic ecosystem, reaching the biota living there, including the fish. Pesticides can affect toxicological parameters these animals, harming their survival. In this way, the aim this study has to evaluate biochemistry changes in carps (Cyprinus carpio) after exposure to a commercial formulation of the fungicide tebuconazole (Folicur®). At first, we performed a test of acute toxicity to determine the LC50 (96h) of tebuconazole for C. carpio. The animals were exposed to concentrations 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L for 96 hours. After this period, were anesthetized and euthanized. Using the whole-body method, we investigated the levels of TBARS, protein carbonyls, activities of enzymes GST, CAT, SOD and AChE, levels of non-enzymatic antioxidants GSH and AsA and metabolic parameters, among which, lactate, glycogen, glucose, ammonia, amino acids and protein. Secondly, fish were exposed to the fungicide during seven days, both in field conditions (rice paddy field) as a laboratory. The concentrations used in the laboratory (33.5 and 36.2 mg/L) were close to the concentration used for rice farming (31.9 mg/L). After the experimental periods, we sampled brain, liver and muscle of fish. The biochemical parameters analyzed were as follows: TBARS levels in brain, liver and muscle, protein carbonyl in liver, enzyme activities CAT and GST in liver and AChE in brain and muscle. In the first experiment was obtained as LC50 (96h) of tebuconazole for C. carpio concentration 2.37 mg/L. The body levels of TBARS showed to be high at all concentrations tested. In contrast, the enzymes GST, CAT and SOD showed reduction in their activities. The levels of GSH and AsA were also decreased after the same period. Regarding the activity of AChE and the levels of protein carbonyls, there were no statistically significant changes. Among the metabolic parameters, there was an increase in the levels of glycogen and glucose concentration 1.5 mg/L, whereas protein levels were reduced at concentrations 2.0 and 2.5 mg/L. In the second experiment, we observed increased levels of TBARS in brain only in the field condition, while in liver and muscle this increase was observed both in field and laboratory. Likewise, the levels of protein carbonyls increased in both experimental conditions. The activity of AChE in brain was increased only in the field condition, whereas in the laboratory no significant change was observed. The activities of CAT, GST and AChE in muscle showed no significant change in both experimental conditions. The results of this research, considering the two experiments show that this fungicide causes disorders in antioxidants and metabolic parameters in fish exposed, indicating the occurrence of oxidative stress, which can compromise their survival in the wild. The evaluation of TBARS levels and protein carbonyl in the liver of carp can be used as a biomarker of exposure to tebuconazole.Conselho Nacional de Desenvolvimento Científico e TecnológicoO intenso uso e/ou manejo inadequado de pesticidas têm levado à contaminação do ecossistema aquático, atingindo a biota ali existente, incluindo os peixes. Pesticidas podem afetar parâmetros toxicológicos desses animais, prejudicando sua sobrevivência. Neste sentido, o objetivo deste trabalho foi avaliar alterações bioquímicas em carpas (Cyprinus carpio) após exposição a uma formulação comercial do fungicida tebuconazole (Folicur®). Num primeiro momento, foi realizado um teste de toxicidade aguda a fim de determinar a CL50 (96h) do tebuconazole para C. carpio. Os animais foram expostos às concentrações 1,0, 1,5, 2,0, 2,5 e 3,0 mg/L, durante 96 horas. Após esse período, foram anestesiados e eutanasiados. Utilizando o método whole-body, foram investigados os níveis de TBARS, proteína carbonil, atividades das enzimas GST, CAT, SOD e AChE, níveis dos antioxidantes não enzimáticos GSH e AsA e parâmetros metabólicos, dentre os quais, lactato, glicogênio, glicose, amônia, aminoácidos e proteína. Num segundo momento, os peixes foram expostos ao fungicida durante sete dias, tanto em condição de campo (lavoura de arroz irrigado) como de laboratório. As concentrações utilizadas em laboratório (33,5 e 36,2 μg/L) foram próximas à concentração utilizada na lavoura de arroz (31,9 μg/L). Decorridos os períodos experimentais, foram amostrados cérebro, fígado e músculo dos peixes. Os parâmetros bioquímicos analisados foram os seguintes: níveis de TBARS em cérebro, fígado e músculo; proteína carbonil em fígado; atividades das enzimas CAT e GST em fígado e da AChE em cérebro e músculo. No primeiro experimento obteve-se como CL50 (96h) do tebuconazole para C. carpio a concentração 2,37 mg/L. Os níveis corporais de TBARS mostraram-se elevados em todas as concentrações testadas. Ao contrário, as enzimas GST, CAT e SOD mostraram redução em suas atividades. Os níveis de GSH e AsA também se mostraram diminuídos após o mesmo período. Em relação à atividade da AChE e aos níveis de proteína carbonil, não foram observadas alterações estatisticamente significativas. Dentre os parâmetros metabólicos, houve aumento nos níveis de glicogênio e glicose na concentração 1,5 mg/L, enquanto que os níveis de proteína foram reduzidos nas concentrações 2,0 e 2,5 mg/L. No segundo experimento, observou-se aumento nos níveis de TBARS em cérebro somente na condição de campo, enquanto que em fígado e músculo esse aumento foi verificado tanto em campo quanto em laboratório. Da mesma forma, os níveis de proteína carbonil aumentaram em ambas as condições experimentais. A atividade da AChE em cérebro se mostrou aumentada somente em condição de campo, enquanto que em laboratório nenhuma alteração significativa foi observada. As atividades da CAT, GST e AChE em músculo não apresentaram mudanças significativas em ambas as condições experimentais. Os resultados da presente investigação, considerando os dois experimentos realizados, mostram que esse fungicida provoca desordens em parâmetros antioxidantes e metabólicos nos peixes expostos, indicando a ocorrência de estresse oxidativo, o que pode comprometer sua sobrevivência no meio natural. A avaliação dos níveis de TBARS e de proteína carbonil em fígado de carpas pode ser utilizada como biomarcador de exposição ao tebuconazole.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaLoro, Vania Luciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7Morsch, Vera Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6Radünz Neto, Joaohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783033E5Toni, Cândida2017-04-202017-04-202010-07-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfTONI, Cândida. Biochemical response in carp (Cyprinus carpio) exposed to fungicide tebuconazole (folicur® 200 ec). 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/11132ark:/26339/001300000s82qporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-25T15:09:40Zoai:repositorio.ufsm.br:1/11132Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T15:09:40Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
Biochemical response in carp (Cyprinus carpio) exposed to fungicide tebuconazole (folicur® 200 ec)
title Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
spellingShingle Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
Toni, Cândida
Cyprinus carpio
Estresse oxidativo
Tebuconazole
Toxicidade
Cyprinus carpio
Oxidative stress
Tebuconazole
Toxicity
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
title_full Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
title_fullStr Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
title_full_unstemmed Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
title_sort Respostas bioquímicas em carpas (Cyprinus carpio) expostas ao fungicida tebuconazole (folicur® 200 ec)
author Toni, Cândida
author_facet Toni, Cândida
author_role author
dc.contributor.none.fl_str_mv Loro, Vania Lucia
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7
Morsch, Vera Maria
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6
Radünz Neto, Joao
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783033E5
dc.contributor.author.fl_str_mv Toni, Cândida
dc.subject.por.fl_str_mv Cyprinus carpio
Estresse oxidativo
Tebuconazole
Toxicidade
Cyprinus carpio
Oxidative stress
Tebuconazole
Toxicity
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Cyprinus carpio
Estresse oxidativo
Tebuconazole
Toxicidade
Cyprinus carpio
Oxidative stress
Tebuconazole
Toxicity
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description The intensive use and/or inadequate management of pesticides have led to contamination of the aquatic ecosystem, reaching the biota living there, including the fish. Pesticides can affect toxicological parameters these animals, harming their survival. In this way, the aim this study has to evaluate biochemistry changes in carps (Cyprinus carpio) after exposure to a commercial formulation of the fungicide tebuconazole (Folicur®). At first, we performed a test of acute toxicity to determine the LC50 (96h) of tebuconazole for C. carpio. The animals were exposed to concentrations 1.0, 1.5, 2.0, 2.5 and 3.0 mg/L for 96 hours. After this period, were anesthetized and euthanized. Using the whole-body method, we investigated the levels of TBARS, protein carbonyls, activities of enzymes GST, CAT, SOD and AChE, levels of non-enzymatic antioxidants GSH and AsA and metabolic parameters, among which, lactate, glycogen, glucose, ammonia, amino acids and protein. Secondly, fish were exposed to the fungicide during seven days, both in field conditions (rice paddy field) as a laboratory. The concentrations used in the laboratory (33.5 and 36.2 mg/L) were close to the concentration used for rice farming (31.9 mg/L). After the experimental periods, we sampled brain, liver and muscle of fish. The biochemical parameters analyzed were as follows: TBARS levels in brain, liver and muscle, protein carbonyl in liver, enzyme activities CAT and GST in liver and AChE in brain and muscle. In the first experiment was obtained as LC50 (96h) of tebuconazole for C. carpio concentration 2.37 mg/L. The body levels of TBARS showed to be high at all concentrations tested. In contrast, the enzymes GST, CAT and SOD showed reduction in their activities. The levels of GSH and AsA were also decreased after the same period. Regarding the activity of AChE and the levels of protein carbonyls, there were no statistically significant changes. Among the metabolic parameters, there was an increase in the levels of glycogen and glucose concentration 1.5 mg/L, whereas protein levels were reduced at concentrations 2.0 and 2.5 mg/L. In the second experiment, we observed increased levels of TBARS in brain only in the field condition, while in liver and muscle this increase was observed both in field and laboratory. Likewise, the levels of protein carbonyls increased in both experimental conditions. The activity of AChE in brain was increased only in the field condition, whereas in the laboratory no significant change was observed. The activities of CAT, GST and AChE in muscle showed no significant change in both experimental conditions. The results of this research, considering the two experiments show that this fungicide causes disorders in antioxidants and metabolic parameters in fish exposed, indicating the occurrence of oxidative stress, which can compromise their survival in the wild. The evaluation of TBARS levels and protein carbonyl in the liver of carp can be used as a biomarker of exposure to tebuconazole.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-22
2017-04-20
2017-04-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv TONI, Cândida. Biochemical response in carp (Cyprinus carpio) exposed to fungicide tebuconazole (folicur® 200 ec). 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.
http://repositorio.ufsm.br/handle/1/11132
dc.identifier.dark.fl_str_mv ark:/26339/001300000s82q
identifier_str_mv TONI, Cândida. Biochemical response in carp (Cyprinus carpio) exposed to fungicide tebuconazole (folicur® 200 ec). 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.
ark:/26339/001300000s82q
url http://repositorio.ufsm.br/handle/1/11132
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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