O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/19216 |
Resumo: | Considered one of the most common neurological diseases, stroke has a high morbidity and mortality, and corresponds to 85% of disease cases. Several factors are involved in disease etiology, such as changes in vascular permeability, oxidative stress, inflammation, apoptosis and DNA damage. Among the main consequences are cognitive and motor deficits, depending on the level and time of injury. The single nucleotide polymorphism Ala16Val (SNP) is a genetic mutation of the antioxidant enzyme superoxide dismutase and is associated with the risk factors of metabolic and vascular diseases, such as stroke. Brain derived neurotrophic (BDNF) is a neurotrofin that, among various functions, is associated with tissue regeneration after cerebrovascular lesions. Acetylcholine (ACh) is a neurotransmitter related to mnemonic processes, learning and motor functions. Thus, this study was to investigate the relationship between the polymorphism Ala16Val with oxidative, inflammatory, apoptotics and DNA damage markers, as well, with levels of BDNF, ACh, memory and functional capacity of chronic stroke patients. Fourthy-eight ischemic stroke patients with and fifthy healthy controls were subjected to questionnaires, laboratory tests and clinical trials. The results showed a higher proportion of the VV genotype in stroke patients compared to healthy individuals. VV genotype patients also showed higher levels of 2 -7 ′ ′ diclorofluorescein diacetate (DCFH-DA), nitrite/nitrate (NOX), tumor necrosis factor-α (TNF-α), acetylcholinesterase (AChE), caspase 8 (CASP 8), caspase 3 (CASP 3) and PicoGreen (PG), as well as decreased levels of BDNF and ACh. Similarly, in cognitive tests (working memory and total memory) and tests (Berg Balance scale and the senior fitness test-STF), VV genotype patients had worse results when compared with the other genotypes (stroke and control groups). In this way, the results suggest a relationship of polymorphism Ala16Val with oxidative/nitrosatve and inflammatory routes, culminated in activation of the apoptotic cascade and DNA damage, especially for VV genotype patients. Moreover, VV patients showed worst results in cognitive and motor tests, as well as low BDNF and ACh levels. Thus, is plausible to suggest that VV patients with present a worse performance in relation to cognitive and motor function, as well as increase in oxidative and inflammatory parameters. Our findings also related clinical changes reduced levels of BDNF and ACh, suggesting that knowledge of Mn-SOD polymorphism is important to evaluate the molecular mechanisms associated with neurovascular lesion and offer a individualized treatment for these patients. |
id |
UFSM_c3c920f288651ea9d3b2baf26694764a |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/19216 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2019-12-23T13:49:18Z2019-12-23T13:49:18Z2019-08-17http://repositorio.ufsm.br/handle/1/19216Considered one of the most common neurological diseases, stroke has a high morbidity and mortality, and corresponds to 85% of disease cases. Several factors are involved in disease etiology, such as changes in vascular permeability, oxidative stress, inflammation, apoptosis and DNA damage. Among the main consequences are cognitive and motor deficits, depending on the level and time of injury. The single nucleotide polymorphism Ala16Val (SNP) is a genetic mutation of the antioxidant enzyme superoxide dismutase and is associated with the risk factors of metabolic and vascular diseases, such as stroke. Brain derived neurotrophic (BDNF) is a neurotrofin that, among various functions, is associated with tissue regeneration after cerebrovascular lesions. Acetylcholine (ACh) is a neurotransmitter related to mnemonic processes, learning and motor functions. Thus, this study was to investigate the relationship between the polymorphism Ala16Val with oxidative, inflammatory, apoptotics and DNA damage markers, as well, with levels of BDNF, ACh, memory and functional capacity of chronic stroke patients. Fourthy-eight ischemic stroke patients with and fifthy healthy controls were subjected to questionnaires, laboratory tests and clinical trials. The results showed a higher proportion of the VV genotype in stroke patients compared to healthy individuals. VV genotype patients also showed higher levels of 2 -7 ′ ′ diclorofluorescein diacetate (DCFH-DA), nitrite/nitrate (NOX), tumor necrosis factor-α (TNF-α), acetylcholinesterase (AChE), caspase 8 (CASP 8), caspase 3 (CASP 3) and PicoGreen (PG), as well as decreased levels of BDNF and ACh. Similarly, in cognitive tests (working memory and total memory) and tests (Berg Balance scale and the senior fitness test-STF), VV genotype patients had worse results when compared with the other genotypes (stroke and control groups). In this way, the results suggest a relationship of polymorphism Ala16Val with oxidative/nitrosatve and inflammatory routes, culminated in activation of the apoptotic cascade and DNA damage, especially for VV genotype patients. Moreover, VV patients showed worst results in cognitive and motor tests, as well as low BDNF and ACh levels. Thus, is plausible to suggest that VV patients with present a worse performance in relation to cognitive and motor function, as well as increase in oxidative and inflammatory parameters. Our findings also related clinical changes reduced levels of BDNF and ACh, suggesting that knowledge of Mn-SOD polymorphism is important to evaluate the molecular mechanisms associated with neurovascular lesion and offer a individualized treatment for these patients.Considerada uma das doenças neurológicas mais frequentes, o acidente vascular encefálico (AVE) apresenta uma alta taxa de morbidade e mortalidade, sendo que o AVE isquêmico corresponde a 85% dos casos. Diversos fatores estão envolvidos na etiologia da doença, como alterações na permeabilidade vascular, estresse oxidativo, inflamação, apoptose e dano ao DNA. Entre outras consequências estão os déficits cognitivos e motores, dependendo da magnitude e tempo da lesão. O polimorfismo de nucleotídeo único MnSOD Ala16Val (SNP) é uma mutação genética da enzima antioxidante superóxido dismutase e está associado a fatores de risco de doenças metabólicas e vasculares, como o AVE. O fator neurotrófico derivado do encéfalo (BDNF) é uma neurotrofina que, entre várias funções, está associada a regeneração tecidual após lesões vasculares cerebrais. A acetilcolina (ACh) é um neurotransmissor excitatório relacionado com processos mnemônicos, aprendizado e funções motoras. Desta forma, foi objetivo deste estudo investigar a relação entre o polimorfismo da Ala16Val MnSOD com os marcadores oxidativos, inflamatórios, apoptóticos e de dano ao DNA, bem como, com os níveis de BDNF, ACh e atividade da AChE, memória e a capacidade funcional de pacientes após-AVE crônico isquêmico (AVEi) crônico. Quarenta e oito pacientes com AVE e 50 controles saudáveis foram submetidos a questionários, exames laboratoriais e testes clínicos. Os resultados mostraram uma maior proporção do genótipo VV nos pacientes após-AVE em comparação com indivíduos saudáveis. Os pacientes com genótipo VV também apresentaram níveis mais elevados de 2′-7′ diacetato de diclorofluoresceina (DCFH-DA), nitrito/nitrato (NOX), fator de necrose tumoral-α (TNF-α), acetilcolinesterase (AChE), caspase 8 (CASP 8), caspase 3 (CASP 3) e PicoGreen (PG), bem como, níveis diminuídos de BDNF e ACh. Similarmente, nos testes cognitivos (memória de trabalho e memória total) e nos testes motores (Escala de Equilíbrio de Berg e o teste fitness sênior - STF), os pacientes com genótipo VV tiveram piores resultados quando comparados com os outros genótipos (grupo AVE e controles). Desta forma, os resultados sugerem uma relação do polimorfismo da MnSOD Ala16Val com as rotas oxidativa/nitrosativa e inflamatória, culminado na ativação da cascata apoptótica e dano a DNA, especialmente para aqueles pacientes com genótipo VV. Além disso, os pacientes (VV) mostraram os piores resultados nos testes cognitivos e motores, assim como, níveis reduzidos de BDNF, ACh e atividade da AChE. Assim, é plausível propor que pacientes com AVEi com genótipo VV apresentam um pior desempenho em relaçõ à função cognitiva e motora, assim como, aumento nos parâmetros oxidativos e inflamatórios. Nossos achados também relacionaram as alterações clínicas aos níveis reduzidos de BDNF e ACh, sugerindo que o conhecimento da existência do polimorfismo é importante para avaliar os mecanismos moleculares associados à lesão neurovascular e oferecer um tratamento individualizado para esses pacientes.porUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAcidente vascular encefálicoEstresse oxidativoInflamaçãoApoptoseDano ao DNAMemóriaFuncionalidadeStrokeOxidative stressInflammationApoptosisDNA damageMemoryFuncionalityCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAO papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínicoThe role of the Ala16Val Mn-SOD polymorphism in chronic stroke: a clinical studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisFighera, Michele Rechiahttp://lattes.cnpq.br/8583392747509231Nascimento, Patrícia Severo dohttp://lattes.cnpq.br/6497514350134284Chitolina, Maria Rosahttp://lattes.cnpq.br/4401319386725357Werner, Maria Fernanda de Paulahttp://lattes.cnpq.br/9053828782958389Barcelos, Rômulo Pillonhttp://lattes.cnpq.br/8887253904142575http://lattes.cnpq.br/5874522737974765Pascotini, Eduardo Tanuri2008000000026008306e5eb-747a-45a0-a023-955603a45dcdaa43a8e8-f120-412e-bb6e-6ff57242c3bd9a9a61b1-bfc2-4d41-b8c7-ce9a4491aaa085b5eba4-d225-48ee-95e3-c53d77dff5540a4c111f-44be-48ed-90bc-198a54d49e04e0fc3677-8ca3-4730-96a1-8ec2ef0bf3c8reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGBT_2019_PASCOTINI_EDUARDO.pdfTES_PPGBT_2019_PASCOTINI_EDUARDO.pdfTese de Doutoradoapplication/pdf2652819http://repositorio.ufsm.br/bitstream/1/19216/1/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf503cfe1f77539aa311b487e410d2ff6eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/19216/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/19216/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTTES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.txtTES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.txtExtracted texttext/plain197082http://repositorio.ufsm.br/bitstream/1/19216/4/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.txt7bf56c2b0eb28bdc23af11af42387734MD54THUMBNAILTES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.jpgTES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.jpgIM Thumbnailimage/jpeg4923http://repositorio.ufsm.br/bitstream/1/19216/5/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.jpgee0314c1e10778b82c37da03f4d1a6d0MD551/192162019-12-24 03:02:26.94oai:repositorio.ufsm.br:1/19216TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU2FudGEgTWFyaWEgKFVGU00pIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHLDtG5pY28gZQplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVGU00gcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbwpwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVUZTTSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU00Kb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlw7pkbyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQcOHw4NPIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ8ONTklPIE9VCkFQT0lPIERFIFVNQSBBR8OKTkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTsODTyBTRUpBIEEgVUZTTQosIFZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNNIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKQpkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcwpjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKBiblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-12-24T06:02:26Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
dc.title.alternative.eng.fl_str_mv |
The role of the Ala16Val Mn-SOD polymorphism in chronic stroke: a clinical study |
title |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
spellingShingle |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico Pascotini, Eduardo Tanuri Acidente vascular encefálico Estresse oxidativo Inflamação Apoptose Dano ao DNA Memória Funcionalidade Stroke Oxidative stress Inflammation Apoptosis DNA damage Memory Funcionality CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
title_full |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
title_fullStr |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
title_full_unstemmed |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
title_sort |
O papel do polimorfismo Ala16Val da Mn-SOD no acidente vascular encefálico crônico: um estudo clínico |
author |
Pascotini, Eduardo Tanuri |
author_facet |
Pascotini, Eduardo Tanuri |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fighera, Michele Rechia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8583392747509231 |
dc.contributor.referee1.fl_str_mv |
Nascimento, Patrícia Severo do |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6497514350134284 |
dc.contributor.referee2.fl_str_mv |
Chitolina, Maria Rosa |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4401319386725357 |
dc.contributor.referee3.fl_str_mv |
Werner, Maria Fernanda de Paula |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9053828782958389 |
dc.contributor.referee4.fl_str_mv |
Barcelos, Rômulo Pillon |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/8887253904142575 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5874522737974765 |
dc.contributor.author.fl_str_mv |
Pascotini, Eduardo Tanuri |
contributor_str_mv |
Fighera, Michele Rechia Nascimento, Patrícia Severo do Chitolina, Maria Rosa Werner, Maria Fernanda de Paula Barcelos, Rômulo Pillon |
dc.subject.por.fl_str_mv |
Acidente vascular encefálico Estresse oxidativo Inflamação Apoptose Dano ao DNA Memória Funcionalidade |
topic |
Acidente vascular encefálico Estresse oxidativo Inflamação Apoptose Dano ao DNA Memória Funcionalidade Stroke Oxidative stress Inflammation Apoptosis DNA damage Memory Funcionality CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Stroke Oxidative stress Inflammation Apoptosis DNA damage Memory Funcionality |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Considered one of the most common neurological diseases, stroke has a high morbidity and mortality, and corresponds to 85% of disease cases. Several factors are involved in disease etiology, such as changes in vascular permeability, oxidative stress, inflammation, apoptosis and DNA damage. Among the main consequences are cognitive and motor deficits, depending on the level and time of injury. The single nucleotide polymorphism Ala16Val (SNP) is a genetic mutation of the antioxidant enzyme superoxide dismutase and is associated with the risk factors of metabolic and vascular diseases, such as stroke. Brain derived neurotrophic (BDNF) is a neurotrofin that, among various functions, is associated with tissue regeneration after cerebrovascular lesions. Acetylcholine (ACh) is a neurotransmitter related to mnemonic processes, learning and motor functions. Thus, this study was to investigate the relationship between the polymorphism Ala16Val with oxidative, inflammatory, apoptotics and DNA damage markers, as well, with levels of BDNF, ACh, memory and functional capacity of chronic stroke patients. Fourthy-eight ischemic stroke patients with and fifthy healthy controls were subjected to questionnaires, laboratory tests and clinical trials. The results showed a higher proportion of the VV genotype in stroke patients compared to healthy individuals. VV genotype patients also showed higher levels of 2 -7 ′ ′ diclorofluorescein diacetate (DCFH-DA), nitrite/nitrate (NOX), tumor necrosis factor-α (TNF-α), acetylcholinesterase (AChE), caspase 8 (CASP 8), caspase 3 (CASP 3) and PicoGreen (PG), as well as decreased levels of BDNF and ACh. Similarly, in cognitive tests (working memory and total memory) and tests (Berg Balance scale and the senior fitness test-STF), VV genotype patients had worse results when compared with the other genotypes (stroke and control groups). In this way, the results suggest a relationship of polymorphism Ala16Val with oxidative/nitrosatve and inflammatory routes, culminated in activation of the apoptotic cascade and DNA damage, especially for VV genotype patients. Moreover, VV patients showed worst results in cognitive and motor tests, as well as low BDNF and ACh levels. Thus, is plausible to suggest that VV patients with present a worse performance in relation to cognitive and motor function, as well as increase in oxidative and inflammatory parameters. Our findings also related clinical changes reduced levels of BDNF and ACh, suggesting that knowledge of Mn-SOD polymorphism is important to evaluate the molecular mechanisms associated with neurovascular lesion and offer a individualized treatment for these patients. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-12-23T13:49:18Z |
dc.date.available.fl_str_mv |
2019-12-23T13:49:18Z |
dc.date.issued.fl_str_mv |
2019-08-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/19216 |
url |
http://repositorio.ufsm.br/handle/1/19216 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
8306e5eb-747a-45a0-a023-955603a45dcd aa43a8e8-f120-412e-bb6e-6ff57242c3bd 9a9a61b1-bfc2-4d41-b8c7-ce9a4491aaa0 85b5eba4-d225-48ee-95e3-c53d77dff554 0a4c111f-44be-48ed-90bc-198a54d49e04 e0fc3677-8ca3-4730-96a1-8ec2ef0bf3c8 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/19216/1/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf http://repositorio.ufsm.br/bitstream/1/19216/2/license_rdf http://repositorio.ufsm.br/bitstream/1/19216/3/license.txt http://repositorio.ufsm.br/bitstream/1/19216/4/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.txt http://repositorio.ufsm.br/bitstream/1/19216/5/TES_PPGBT_2019_PASCOTINI_EDUARDO.pdf.jpg |
bitstream.checksum.fl_str_mv |
503cfe1f77539aa311b487e410d2ff6e 4460e5956bc1d1639be9ae6146a50347 2f0571ecee68693bd5cd3f17c1e075df 7bf56c2b0eb28bdc23af11af42387734 ee0314c1e10778b82c37da03f4d1a6d0 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1801485329736138752 |