Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18201 |
Resumo: | Exposition to toxic compounds is becoming more frequent. This fact is of relevant since xenobiotics cause an imbalance in the body homeostasis mainly inducing oxidative stress. In addition, they can alter enzyme activities and gene expression, especially of those related to the antioxidant system and the inflammatory response, which can be used as an indicator of toxicity. Among these compounds vinylcyclohexene (VCH), a byproduct of plastic and derivatives production, and methylmercury (MeHg), mainly found in fish, stand out. We are constantly exposed to a wide range of xenobiotics and it is necessary to study the effects of exposure to toxic agents concomitantly. Thus, this work aims to study the isolated and interactive effects of MeHg and VCH on the oxidative stress and gene modulation in Drosophila melanogaster, since these have similarities in their mechanisms of toxicity. We used different concentrations of MeHg (0 a 400 μM) and VCH (0 a 10 mM) to make survival curves. The highest concentrations of the compounds that did not alter fly survival were used in the interactive effect study. Reactive species production and glutathione S-transferase (GST) and acetylcholinesterase (AChE) enzymes activities were evaluated in the head and in the body of the flies one and three days after individual and concomitant exposure to VCH and MeHg. Changes in gene expression related to redox state and inflammatory cell responses were evaluated after three days of exposure in both tissues. There was a decrease in reactive species production after one day of exposure to MeHg in the head of the flies, in contrast, all treatments increased the reactive species production in the body of the flies after one day of exposure and only the coexposition of the compounds induced an increase in this parameter after three days. Regarding to GST, after one day of exposure to VCH there was an increased the activity of this enzyme whereas exposure to MeHg induced this increased after three days of exposure in the head of the flies. In the body, all treatments increased GST activity after one day of exposure to compounds and after three days, only exposure to MeHg increased the activity of this enzyme. The treatments did not alter AChE activity in the head of the flies. There was an increase in Relish gene expression after the exposure to MeHg in the body of the flies. Therefore, the exposure to VCH and MeHg induced oxidative stress and activation of the inflammatory response. We emphasize that biochemical and molecular tests were able to detect changes even when the mortality rate of the flies was not affected. In most tests we failed to detect an increase in toxic effects after co-exposure, which may be due to the fact that the two compounds tested, despite similarities in the mechanisms of toxicity, have different toxicity targets. |
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Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecularSimultaneous exposure to vinilcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analysisEstresse oxidativoResposta inflamatóriaXenobióticoModelo alternativoOxidative stressInflammatory responseXenobioticAlternative modelCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAExposition to toxic compounds is becoming more frequent. This fact is of relevant since xenobiotics cause an imbalance in the body homeostasis mainly inducing oxidative stress. In addition, they can alter enzyme activities and gene expression, especially of those related to the antioxidant system and the inflammatory response, which can be used as an indicator of toxicity. Among these compounds vinylcyclohexene (VCH), a byproduct of plastic and derivatives production, and methylmercury (MeHg), mainly found in fish, stand out. We are constantly exposed to a wide range of xenobiotics and it is necessary to study the effects of exposure to toxic agents concomitantly. Thus, this work aims to study the isolated and interactive effects of MeHg and VCH on the oxidative stress and gene modulation in Drosophila melanogaster, since these have similarities in their mechanisms of toxicity. We used different concentrations of MeHg (0 a 400 μM) and VCH (0 a 10 mM) to make survival curves. The highest concentrations of the compounds that did not alter fly survival were used in the interactive effect study. Reactive species production and glutathione S-transferase (GST) and acetylcholinesterase (AChE) enzymes activities were evaluated in the head and in the body of the flies one and three days after individual and concomitant exposure to VCH and MeHg. Changes in gene expression related to redox state and inflammatory cell responses were evaluated after three days of exposure in both tissues. There was a decrease in reactive species production after one day of exposure to MeHg in the head of the flies, in contrast, all treatments increased the reactive species production in the body of the flies after one day of exposure and only the coexposition of the compounds induced an increase in this parameter after three days. Regarding to GST, after one day of exposure to VCH there was an increased the activity of this enzyme whereas exposure to MeHg induced this increased after three days of exposure in the head of the flies. In the body, all treatments increased GST activity after one day of exposure to compounds and after three days, only exposure to MeHg increased the activity of this enzyme. The treatments did not alter AChE activity in the head of the flies. There was an increase in Relish gene expression after the exposure to MeHg in the body of the flies. Therefore, the exposure to VCH and MeHg induced oxidative stress and activation of the inflammatory response. We emphasize that biochemical and molecular tests were able to detect changes even when the mortality rate of the flies was not affected. In most tests we failed to detect an increase in toxic effects after co-exposure, which may be due to the fact that the two compounds tested, despite similarities in the mechanisms of toxicity, have different toxicity targets.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA exposição a compostos tóxicos é cada vez mais frequente. Este fato é relevante, visto que xenobióticos causam um desequilíbrio na homeostase do organismo, principalmente, induzindo o estresse oxidativo. Além disso, podem alterar a atividade de enzimas e a expressão gênica, principalmente relacionada ao sistema antioxidante e a resposta inflamatória, o que pode ser usado como um indicador precoce de toxicidade. Dentre estes compostos destacam-se o vinilciclohexeno (VCH), um subproduto da produção de plástico e derivados, e o metilmercúrio (MeHg), uma forma orgânica do Hg, encontrada principalmente nos peixes. Como estamos constantemente expostos a uma ampla variedade de xenobióticos, torna-se necessário estudar os efeitos da exposição a dois ou mais agentes tóxicos concomitantemente. Deste modo, este trabalho visa estudar os efeitos isolados e da interação do MeHg e do VCH frente ao estresse oxidativo e modulação gênica em Drosophila melanogaster, uma vez que estes compostos apresentam semelhanças nos seus mecanismos de toxicidade. Para construir curvas de sobrevivência as moscas foram expostas a diferentes concentrações de MeHg (0 a 400 μM) e de VCH (0 a 10 mM). As maiores concentrações dos compostos que não alteraram a sobrevivência das moscas foram utilizadas no estudo do efeito da coexposição. A produção de espécies reativas (ER) e a atividade das enzimas glutationa S-transferase (GST) e acetilcolinesterase (AChE) foram avaliadas na cabeça e no corpo das moscas após um e três dias de exposição individual e concomitante ao VCH e ao MeHg. As modificações na expressão de genes relacionados à manutenção do estado redox e resposta inflamatória das células foram avaliadas após três dias de exposição em ambos os tecidos. Houve uma diminuição na produção de ER após um dia de exposição ao MeHg na cabeça das moscas, em contraste, todos os tratamentos aumentaram a produção de ER no corpo das moscas após um dia de exposição e somente a coexposição dos compostos induziu um aumento neste parâmetro após três dias. Quanto a GST, na cabeça das moscas, após um dia de exposição ao VCH houve um aumentou na atividade desta enzima ao passo que a exposição ao MeHg induziu este aumento após três dias de exposição. No corpo, todos os tratamentos aumentaram a atividade da GST após um dia de exposição aos compostos e, após três dias, somente a exposição ao MeHg aumentou a atividade desta enzima. Os tratamentos não alteraram a atividade da AChE na cabeça das moscas Ocorreu um aumento na expressão do gene Relish, na exposição ao MeHg no corpo das moscas. Sendo assim, a exposição ao VCH e ao MeHg induziu estresse oxidativo e ativação da resposta inflamatória. Destacamos que os testes bioquímicos e moleculares foram capazes de detectar alterações mesmo quando a taxa de mortalidade das moscas não foi afetada. Na maioria dos testes não conseguimos detectar um aumento nos efeitos tóxicos após a coexposição, o que pode incorrer do fato que os dois compostos testados, apesar das semelhanças nos mecanismos de toxicidade, têm como principais alvos órgãos diferentes.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasRocha, João Batista Teixeira dahttp://lattes.cnpq.br/3935055744673018Segatto, Ana Lúcia Anversahttp://lattes.cnpq.br/9643054385703621Dalla Corte, Cristiane Lenzhttp://lattes.cnpq.br/5296284169605317Wallau, Gabriel da Luzhttp://lattes.cnpq.br/3895703957304351Piccoli, Bruna Candia2019-09-11T21:51:46Z2019-09-11T21:51:46Z2017-02-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/18201porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-08-25T15:22:26Zoai:repositorio.ufsm.br:1/18201Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-25T15:22:26Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular Simultaneous exposure to vinilcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analysis |
title |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
spellingShingle |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular Piccoli, Bruna Candia Estresse oxidativo Resposta inflamatória Xenobiótico Modelo alternativo Oxidative stress Inflammatory response Xenobiotic Alternative model CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
title_full |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
title_fullStr |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
title_full_unstemmed |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
title_sort |
Exposição simultânea ao vinilciclohexeno e ao metilmercúrio em Drosophila melanogaster: análise bioquímica e molecular |
author |
Piccoli, Bruna Candia |
author_facet |
Piccoli, Bruna Candia |
author_role |
author |
dc.contributor.none.fl_str_mv |
Rocha, João Batista Teixeira da http://lattes.cnpq.br/3935055744673018 Segatto, Ana Lúcia Anversa http://lattes.cnpq.br/9643054385703621 Dalla Corte, Cristiane Lenz http://lattes.cnpq.br/5296284169605317 Wallau, Gabriel da Luz http://lattes.cnpq.br/3895703957304351 |
dc.contributor.author.fl_str_mv |
Piccoli, Bruna Candia |
dc.subject.por.fl_str_mv |
Estresse oxidativo Resposta inflamatória Xenobiótico Modelo alternativo Oxidative stress Inflammatory response Xenobiotic Alternative model CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Estresse oxidativo Resposta inflamatória Xenobiótico Modelo alternativo Oxidative stress Inflammatory response Xenobiotic Alternative model CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Exposition to toxic compounds is becoming more frequent. This fact is of relevant since xenobiotics cause an imbalance in the body homeostasis mainly inducing oxidative stress. In addition, they can alter enzyme activities and gene expression, especially of those related to the antioxidant system and the inflammatory response, which can be used as an indicator of toxicity. Among these compounds vinylcyclohexene (VCH), a byproduct of plastic and derivatives production, and methylmercury (MeHg), mainly found in fish, stand out. We are constantly exposed to a wide range of xenobiotics and it is necessary to study the effects of exposure to toxic agents concomitantly. Thus, this work aims to study the isolated and interactive effects of MeHg and VCH on the oxidative stress and gene modulation in Drosophila melanogaster, since these have similarities in their mechanisms of toxicity. We used different concentrations of MeHg (0 a 400 μM) and VCH (0 a 10 mM) to make survival curves. The highest concentrations of the compounds that did not alter fly survival were used in the interactive effect study. Reactive species production and glutathione S-transferase (GST) and acetylcholinesterase (AChE) enzymes activities were evaluated in the head and in the body of the flies one and three days after individual and concomitant exposure to VCH and MeHg. Changes in gene expression related to redox state and inflammatory cell responses were evaluated after three days of exposure in both tissues. There was a decrease in reactive species production after one day of exposure to MeHg in the head of the flies, in contrast, all treatments increased the reactive species production in the body of the flies after one day of exposure and only the coexposition of the compounds induced an increase in this parameter after three days. Regarding to GST, after one day of exposure to VCH there was an increased the activity of this enzyme whereas exposure to MeHg induced this increased after three days of exposure in the head of the flies. In the body, all treatments increased GST activity after one day of exposure to compounds and after three days, only exposure to MeHg increased the activity of this enzyme. The treatments did not alter AChE activity in the head of the flies. There was an increase in Relish gene expression after the exposure to MeHg in the body of the flies. Therefore, the exposure to VCH and MeHg induced oxidative stress and activation of the inflammatory response. We emphasize that biochemical and molecular tests were able to detect changes even when the mortality rate of the flies was not affected. In most tests we failed to detect an increase in toxic effects after co-exposure, which may be due to the fact that the two compounds tested, despite similarities in the mechanisms of toxicity, have different toxicity targets. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-13 2019-09-11T21:51:46Z 2019-09-11T21:51:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18201 |
url |
http://repositorio.ufsm.br/handle/1/18201 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922093098860544 |