Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas

Detalhes bibliográficos
Autor(a) principal: Zdanski, Scheila Fernanda Rockembach
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000vh19
Texto Completo: http://repositorio.ufsm.br/handle/1/5709
Resumo: The sausage is a product of high consumption, but due to its manufacturing process technology, it becomes susceptible to microbiological contamination. To ensure the life of the sausage and their microbiological safety, means of minimizing contamination levels, limiting or preventing microbial growth. Therefore, the objective of this study was to add antimicrobials in the formulation and post-heat treatment, and evaluate their effects on growth of mesophilic aerobic bacteria, lactic acid bacteria and Listeria monocytogenes in sausages vacuum packed, stored at 5 º C during storage of 35 days. We evaluated four treatments: (T1-2.5% solution of sodium lactate in the formulation and immersion in 200 mg / l of nisin solution, T2 - 2.5% solution of sodium lactate in the formulation and immersion 200 mg / l of nisin solution followed by 2.5% solution of lactic acid, T3 - 2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution; T4-2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution followed by 2.5% lactic acid solution) and a control (no antimicrobial was added in formulation and post-thermal processing). There were microbiological (lactic acid bacteria, aerobic mesophilic, and Listeria monocytogenes) and physico-chemical (pH). At the end of 35 days storage there was a synergy between antimicrobials used in the formulation and post-heat treatment, reducing the total count of mesophilic and lactic acid bacteria, as compared to control, and the (T4), who used the formulating a mixture of potassium lactate and sodium diacetate, with the subsequent nisin followed by immersion in lactic acid more effective. The treatments that were used nisin (T1 e T3), after the heat treatment, were not effective in controlling aerobic mesophilic and lactic acid bacteria. Listeria monocytogenes was identified in all control samples during the 35 days of storage. Thus, the synergistic action of antibiotics added to the formulation, and post-thermal processing are obstacles that had a potential to control the growth of mesophilic aerobic bacteria and lactic acid bacteria and especially able to inhibit the growth of Listeria monocytogenes in vacuum-packed sausages and stored at 5 ° C for 35 days of storage.
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spelling Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichasOrganic acids and their salts and nisin in control of lactic acid bacteria, listeria monocytogenes and mesophilic aerobic sausage.SalsichaÁcidos orgânicos e seus saisNisinaSausageOrganic acids and their saltsDiacetateNisinCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSThe sausage is a product of high consumption, but due to its manufacturing process technology, it becomes susceptible to microbiological contamination. To ensure the life of the sausage and their microbiological safety, means of minimizing contamination levels, limiting or preventing microbial growth. Therefore, the objective of this study was to add antimicrobials in the formulation and post-heat treatment, and evaluate their effects on growth of mesophilic aerobic bacteria, lactic acid bacteria and Listeria monocytogenes in sausages vacuum packed, stored at 5 º C during storage of 35 days. We evaluated four treatments: (T1-2.5% solution of sodium lactate in the formulation and immersion in 200 mg / l of nisin solution, T2 - 2.5% solution of sodium lactate in the formulation and immersion 200 mg / l of nisin solution followed by 2.5% solution of lactic acid, T3 - 2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution; T4-2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution followed by 2.5% lactic acid solution) and a control (no antimicrobial was added in formulation and post-thermal processing). There were microbiological (lactic acid bacteria, aerobic mesophilic, and Listeria monocytogenes) and physico-chemical (pH). At the end of 35 days storage there was a synergy between antimicrobials used in the formulation and post-heat treatment, reducing the total count of mesophilic and lactic acid bacteria, as compared to control, and the (T4), who used the formulating a mixture of potassium lactate and sodium diacetate, with the subsequent nisin followed by immersion in lactic acid more effective. The treatments that were used nisin (T1 e T3), after the heat treatment, were not effective in controlling aerobic mesophilic and lactic acid bacteria. Listeria monocytogenes was identified in all control samples during the 35 days of storage. Thus, the synergistic action of antibiotics added to the formulation, and post-thermal processing are obstacles that had a potential to control the growth of mesophilic aerobic bacteria and lactic acid bacteria and especially able to inhibit the growth of Listeria monocytogenes in vacuum-packed sausages and stored at 5 ° C for 35 days of storage.A salsicha é um produto de elevado consumo, porém devido ao seu processo tecnológico de fabricação, torna-se susceptível a contaminação microbiológica. Garantir a vida útil da salsicha e a sua segurança microbiológica, implica em minimizar níveis de contaminação, limitando ou impedindo o crescimento microbiano. Portanto, o objetivo deste trabalho foi adicionar antimicrobianos na formulação e no pós-tratamento térmico, e avaliar os efeitos no desenvolvimento das bactérias aeróbias mesófilas, láticas e da Listeria monocytogenes em salsichas embaladas a vácuo, armazenadas a temperatura de 5ºC durante o período de armazenamento de 35 dias. Avaliaram-se quatro tratamentos: (T1- 2,5% de solução de lactato de sódio na formulação e imersão em 200 mg/l de solução de nisina;T2 - 2,5% de solução de lactato de sódio na formulação e imersão em 200 mg/l de solução de nisina seguida de 2,5% de solução de ácido lático;T3 - 2,5% de solução de lactato de potássio e diacetato de sódio na formulação e imersão em 200 mg/l de solução de nisina;T4- 2,5% de solução de lactato de potássio e diacetato de sódio na formulação e imersão em 200 mg/l de solução de nisina seguida de 2,5% de solução de ácido lático) e um controle (não foi adicionado antimicrobianos na formulação e no pós-processamento térmico).Realizaram-se análises microbiológicas (bactérias láticas, aeróbias mesófilas e Listeria monocytogenes) e físico-química (pH). Ao final de 35 dias de armazenamento verificou-se uma sinergia entre os antimicrobianos utilizados na formulação e no pós-tratamento térmico, reduzindo a contagem total de microrganismos mesófilos e bactérias láticas, em relação ao controle, sendo o (T4), que utilizou na formulação a mistura de lactato de potássio e diacetato de sódio, com a posterior imersão em nisina seguida de ácido lático o mais eficaz. Os tratamentos em que foram utilizados a nisina (T1 e T3), no pós-tratamento térmico, não foram eficazes no controle de bactérias aeróbias mesófilas e láticas. A Listeria monocytogenes, foi identificada em todas as amostras controle, durante os 35 dias de armazenamento. Porém, os antimicrobianos adicionados na formulação e no pós-tratamento térmico foram eficazes para prevenir o crescimento do patógeno, com exceção do T1. Desta forma a ação sinergistica de antimicrobianos adicionados na formulação, e no pós-processamento térmico são obstáculos que apresentaram um potencial no controle do crescimento de bactérias aeróbias mesófilas e bactérias láticas e principalmente capazes de inibir o crescimento de Listeria monocytogenes em salsichas embaladas a vácuo e armazenadas a 5ºC por 35 dias de armazenamento.Universidade Federal de Santa MariaBRCiência e Tecnologia dos AlimentosUFSMPrograma de Pós-Graduação em Ciência e Tecnologia dos AlimentosFries, Leadir Lucy Martinshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781614A3Cichoski, Alexandre Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794168U5Sawitzki, Maristela Cortezhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708067H6Zdanski, Scheila Fernanda Rockembach2012-08-272012-08-272011-08-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfZDANSKI, Scheila Fernanda Rockembach. ORGANIC ACIDS AND THEIR SALTS AND NISIN IN CONTROL OF LACTIC ACID BACTERIA, listeria monocytogenes AND MESOPHILIC AEROBIC SAUSAGE.. 2011. 63 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/5709ark:/26339/001300000vh19porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-03-04T18:58:22Zoai:repositorio.ufsm.br:1/5709Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-03-04T18:58:22Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
Organic acids and their salts and nisin in control of lactic acid bacteria, listeria monocytogenes and mesophilic aerobic sausage.
title Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
spellingShingle Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
Zdanski, Scheila Fernanda Rockembach
Salsicha
Ácidos orgânicos e seus sais
Nisina
Sausage
Organic acids and their salts
Diacetate
Nisin
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
title_full Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
title_fullStr Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
title_full_unstemmed Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
title_sort Ácidos orgânicos e seus sais e nisina no controle de bactérias láticas, aeróbias mesófilas e listeria monocytogenes em salsichas
author Zdanski, Scheila Fernanda Rockembach
author_facet Zdanski, Scheila Fernanda Rockembach
author_role author
dc.contributor.none.fl_str_mv Fries, Leadir Lucy Martins
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781614A3
Cichoski, Alexandre José
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794168U5
Sawitzki, Maristela Cortez
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708067H6
dc.contributor.author.fl_str_mv Zdanski, Scheila Fernanda Rockembach
dc.subject.por.fl_str_mv Salsicha
Ácidos orgânicos e seus sais
Nisina
Sausage
Organic acids and their salts
Diacetate
Nisin
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
topic Salsicha
Ácidos orgânicos e seus sais
Nisina
Sausage
Organic acids and their salts
Diacetate
Nisin
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description The sausage is a product of high consumption, but due to its manufacturing process technology, it becomes susceptible to microbiological contamination. To ensure the life of the sausage and their microbiological safety, means of minimizing contamination levels, limiting or preventing microbial growth. Therefore, the objective of this study was to add antimicrobials in the formulation and post-heat treatment, and evaluate their effects on growth of mesophilic aerobic bacteria, lactic acid bacteria and Listeria monocytogenes in sausages vacuum packed, stored at 5 º C during storage of 35 days. We evaluated four treatments: (T1-2.5% solution of sodium lactate in the formulation and immersion in 200 mg / l of nisin solution, T2 - 2.5% solution of sodium lactate in the formulation and immersion 200 mg / l of nisin solution followed by 2.5% solution of lactic acid, T3 - 2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution; T4-2.5% solution of potassium lactate and sodium diacetate in formulation and immersion in 200 mg / l of nisin solution followed by 2.5% lactic acid solution) and a control (no antimicrobial was added in formulation and post-thermal processing). There were microbiological (lactic acid bacteria, aerobic mesophilic, and Listeria monocytogenes) and physico-chemical (pH). At the end of 35 days storage there was a synergy between antimicrobials used in the formulation and post-heat treatment, reducing the total count of mesophilic and lactic acid bacteria, as compared to control, and the (T4), who used the formulating a mixture of potassium lactate and sodium diacetate, with the subsequent nisin followed by immersion in lactic acid more effective. The treatments that were used nisin (T1 e T3), after the heat treatment, were not effective in controlling aerobic mesophilic and lactic acid bacteria. Listeria monocytogenes was identified in all control samples during the 35 days of storage. Thus, the synergistic action of antibiotics added to the formulation, and post-thermal processing are obstacles that had a potential to control the growth of mesophilic aerobic bacteria and lactic acid bacteria and especially able to inhibit the growth of Listeria monocytogenes in vacuum-packed sausages and stored at 5 ° C for 35 days of storage.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-29
2012-08-27
2012-08-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv ZDANSKI, Scheila Fernanda Rockembach. ORGANIC ACIDS AND THEIR SALTS AND NISIN IN CONTROL OF LACTIC ACID BACTERIA, listeria monocytogenes AND MESOPHILIC AEROBIC SAUSAGE.. 2011. 63 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2011.
http://repositorio.ufsm.br/handle/1/5709
dc.identifier.dark.fl_str_mv ark:/26339/001300000vh19
identifier_str_mv ZDANSKI, Scheila Fernanda Rockembach. ORGANIC ACIDS AND THEIR SALTS AND NISIN IN CONTROL OF LACTIC ACID BACTERIA, listeria monocytogenes AND MESOPHILIC AEROBIC SAUSAGE.. 2011. 63 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2011.
ark:/26339/001300000vh19
url http://repositorio.ufsm.br/handle/1/5709
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Ciência e Tecnologia dos Alimentos
UFSM
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Ciência e Tecnologia dos Alimentos
UFSM
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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