Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/0013000001g5z |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11250 |
Resumo: | Type 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes. |
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Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratosEvaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic ratsAlecrimBatataColinesterasesDiabetesLinfócitosCholinesterasesDiabetesLymphocytesPotatoRosemaryCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAType 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO diabetes tipo 1 (DT1) é uma doença caracterizada pela incapacidade do indivíduo de produzir o hormônio insulina devido à destruição das células-β pancreáticas pelas células imune do próprio organismo. Ainda não existe uma terapia 100 % eficaz para tratar os sintomas do DT1. Os tratamentos atuais envolvem monitoramento constante dos níveis de glicose do sangue e injeções periódicas de insulina. Os processos inflamatórios estão associados com o desenvolvimento e a progressão do DT1 e, por isso, novas terapias envolvendo fármacos com propriedades anti-inflamatórias têm sido alvo crescente de interesse no meio científico. Os polifenois são uma classe de compostos heterocíclicos dos quais fazem parte as antocianinas e o ácido rosmarínico, conhecidos por suas propriedades antioxidantes e anti-inflamatórias. Neste trabalho, estudamos os efeitos de dois extratos ricos em polifenois batata cozida e alecrim - e de um concentrado de antocianinas em um modelo de ratos com diabetes tipo 1 induzido por estreptozotocina. Os extratos foram administrados por gavagem durante 30 dias. Após, os ratos foram eutanasiados e verificou-se os efeitos do tratamento sobre a atividade da acetilcolinesterase (AChE) de linfócitos e da butirilcolinesterase (BuChE) de soro, devido à importância dessas enzimas como biomarcadores de inflamação. As atividades de ambas as enzimas mostraram-se aumentadas nos ratos diabéticos em relação aos controles. O extrato de casca de batata cozida e as antocianinas concentradas não alteraram significativamente a atividade da AChE em linfócitos, provavelmente devido à sua baixa biodisponibilidade, enquanto o extrato de alecrim foi um potente inibidor dessa enzima. Nenhum dos tratamentos surtiu efeito sobre a atividade da BuChE de soro. Estes resultados reforçam a hipótese inflamatória na patologia do DT1 e apresentam o extrato de alecrim como uma possível terapia secundária para o tratamento de doenças associadas à inflamação, como o diabetes tipo 1.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaSchetinger, Maria Rosa Chitolinahttp://lattes.cnpq.br/4401319386725357Rosemberg, Denis Broockhttp://lattes.cnpq.br/7713953979203056Oliveira, Sara Marchesan dehttp://lattes.cnpq.br/6574555059806902Dutra, Eduardo José Machado2016-10-132016-10-132014-12-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfDUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/11250ark:/26339/0013000001g5zporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-05-02T19:31:10Zoai:repositorio.ufsm.br:1/11250Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:18:32.887608Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats |
title |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
spellingShingle |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos Dutra, Eduardo José Machado Alecrim Batata Colinesterases Diabetes Linfócitos Cholinesterases Diabetes Lymphocytes Potato Rosemary CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
title_full |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
title_fullStr |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
title_full_unstemmed |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
title_sort |
Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos |
author |
Dutra, Eduardo José Machado |
author_facet |
Dutra, Eduardo José Machado |
author_role |
author |
dc.contributor.none.fl_str_mv |
Schetinger, Maria Rosa Chitolina http://lattes.cnpq.br/4401319386725357 Rosemberg, Denis Broock http://lattes.cnpq.br/7713953979203056 Oliveira, Sara Marchesan de http://lattes.cnpq.br/6574555059806902 |
dc.contributor.author.fl_str_mv |
Dutra, Eduardo José Machado |
dc.subject.por.fl_str_mv |
Alecrim Batata Colinesterases Diabetes Linfócitos Cholinesterases Diabetes Lymphocytes Potato Rosemary CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Alecrim Batata Colinesterases Diabetes Linfócitos Cholinesterases Diabetes Lymphocytes Potato Rosemary CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Type 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-19 2016-10-13 2016-10-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
DUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/11250 |
dc.identifier.dark.fl_str_mv |
ark:/26339/0013000001g5z |
identifier_str_mv |
DUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. ark:/26339/0013000001g5z |
url |
http://repositorio.ufsm.br/handle/1/11250 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1814439710622220288 |