Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos

Detalhes bibliográficos
Autor(a) principal: Dutra, Eduardo José Machado
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/0013000001g5z
Texto Completo: http://repositorio.ufsm.br/handle/1/11250
Resumo: Type 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes.
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spelling Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratosEvaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic ratsAlecrimBatataColinesterasesDiabetesLinfócitosCholinesterasesDiabetesLymphocytesPotatoRosemaryCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAType 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO diabetes tipo 1 (DT1) é uma doença caracterizada pela incapacidade do indivíduo de produzir o hormônio insulina devido à destruição das células-β pancreáticas pelas células imune do próprio organismo. Ainda não existe uma terapia 100 % eficaz para tratar os sintomas do DT1. Os tratamentos atuais envolvem monitoramento constante dos níveis de glicose do sangue e injeções periódicas de insulina. Os processos inflamatórios estão associados com o desenvolvimento e a progressão do DT1 e, por isso, novas terapias envolvendo fármacos com propriedades anti-inflamatórias têm sido alvo crescente de interesse no meio científico. Os polifenois são uma classe de compostos heterocíclicos dos quais fazem parte as antocianinas e o ácido rosmarínico, conhecidos por suas propriedades antioxidantes e anti-inflamatórias. Neste trabalho, estudamos os efeitos de dois extratos ricos em polifenois batata cozida e alecrim - e de um concentrado de antocianinas em um modelo de ratos com diabetes tipo 1 induzido por estreptozotocina. Os extratos foram administrados por gavagem durante 30 dias. Após, os ratos foram eutanasiados e verificou-se os efeitos do tratamento sobre a atividade da acetilcolinesterase (AChE) de linfócitos e da butirilcolinesterase (BuChE) de soro, devido à importância dessas enzimas como biomarcadores de inflamação. As atividades de ambas as enzimas mostraram-se aumentadas nos ratos diabéticos em relação aos controles. O extrato de casca de batata cozida e as antocianinas concentradas não alteraram significativamente a atividade da AChE em linfócitos, provavelmente devido à sua baixa biodisponibilidade, enquanto o extrato de alecrim foi um potente inibidor dessa enzima. Nenhum dos tratamentos surtiu efeito sobre a atividade da BuChE de soro. Estes resultados reforçam a hipótese inflamatória na patologia do DT1 e apresentam o extrato de alecrim como uma possível terapia secundária para o tratamento de doenças associadas à inflamação, como o diabetes tipo 1.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaSchetinger, Maria Rosa Chitolinahttp://lattes.cnpq.br/4401319386725357Rosemberg, Denis Broockhttp://lattes.cnpq.br/7713953979203056Oliveira, Sara Marchesan dehttp://lattes.cnpq.br/6574555059806902Dutra, Eduardo José Machado2016-10-132016-10-132014-12-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfDUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/11250ark:/26339/0013000001g5zporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-05-02T19:31:10Zoai:repositorio.ufsm.br:1/11250Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:18:32.887608Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats
title Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
spellingShingle Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
Dutra, Eduardo José Machado
Alecrim
Batata
Colinesterases
Diabetes
Linfócitos
Cholinesterases
Diabetes
Lymphocytes
Potato
Rosemary
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
title_full Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
title_fullStr Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
title_full_unstemmed Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
title_sort Avaliação do efeito de extratos de batata e alecrim sobre as colinesterases em um modelo de diabetes tipo 1 em ratos
author Dutra, Eduardo José Machado
author_facet Dutra, Eduardo José Machado
author_role author
dc.contributor.none.fl_str_mv Schetinger, Maria Rosa Chitolina
http://lattes.cnpq.br/4401319386725357
Rosemberg, Denis Broock
http://lattes.cnpq.br/7713953979203056
Oliveira, Sara Marchesan de
http://lattes.cnpq.br/6574555059806902
dc.contributor.author.fl_str_mv Dutra, Eduardo José Machado
dc.subject.por.fl_str_mv Alecrim
Batata
Colinesterases
Diabetes
Linfócitos
Cholinesterases
Diabetes
Lymphocytes
Potato
Rosemary
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Alecrim
Batata
Colinesterases
Diabetes
Linfócitos
Cholinesterases
Diabetes
Lymphocytes
Potato
Rosemary
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Type 1 diabetes (T1D) is a disease characterized by the individual's inability to produce the hormone insulin due to destruction of pancreatic β-cells by the immune cells of the organism itself. So far, there is no 100% effective therapy for treating the symptoms of T1D. Current treatments involve constant monitoring of blood glucose levels and regular insulin injections. Inflammatory processes are associated with the development and progression of T1D, and so new therapies involving drugs with anti-inflammatory properties have been the subject of increasing interest in the scientific community. Polyphenols are a class of heterocyclic compounds that include anthocyanins and rosmarinic acid, both known for their antioxidant and anti-inflammatory properties. We studied the effects of two polyphenol rich extracts (cooked potato and rosemary) and a concentrate of anthocyanins in a model of type 1 streptozotocin-induced diabetic rats. The extracts were administered by gavage for 30 days. After this period, the rats were euthanized and we assessed acetylcholinesterase (AChE) activity in lymphocytes and serum butyrylcholinesterase (BuChE) activity, because of the importance of these enzymes as biomarkers of inflammation. The activities of both enzymes were shown to be increased in diabetic rats compared to controls. The cooked potato peel extract and concentrated anthocyanins did not altered AChE activity in lymphocytes, probably due to low bioavailability, while rosemary extract was a potent inhibitor and reverted the activity to normal levels. None of the treatments had any effect on serum BuChE activity. These results support the involvement of inflammatory processes on TD1 pathology and present the rosemary extracts as a promising alternative for future phytochemical therapies for the treatment of inflammatory-related diseases, such as type 1 diabetes.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-19
2016-10-13
2016-10-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv DUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
http://repositorio.ufsm.br/handle/1/11250
dc.identifier.dark.fl_str_mv ark:/26339/0013000001g5z
identifier_str_mv DUTRA, Eduardo José Machado. Evaluation of the effects of potato and rosemary extracts on cholinesterases of type-1 diabetic rats. 2014. 60 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
ark:/26339/0013000001g5z
url http://repositorio.ufsm.br/handle/1/11250
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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